hydroxymethylated loci genomic dna  (Qiagen)


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    Qiagen hydroxymethylated loci genomic dna
    MspI and HpaII isoschizomer can distinguish between 5-hmC and 5-ghmC at the internal cytosine residue. Cleavage specificity is shown for MspI and HpaII on FAM-end-labeled oligonucleotide duplex with internal CG being symmetrically <t>hydroxymethylated</t> or hemihydroxymethylated in the presence or absence of β-GT. Complete cleavage products are labeled as 24 and 19 nt, respectively, on a non-denaturing acrylamide gel for double-hydroxymethylated ( upper panel ) and denaturing acrylamide gel for hemi-hydroxymethylated <t>DNA</t> ( lower panel ). The arrow at the right indicates small amounts of 24-nt-long product.
    Hydroxymethylated Loci Genomic Dna, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hydroxymethylated loci genomic dna/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hydroxymethylated loci genomic dna - by Bioz Stars, 2021-06
    86/100 stars

    Images

    1) Product Images from "Tissue-specific Distribution and Dynamic Changes of 5-Hydroxymethylcytosine in Mammalian Genomes *"

    Article Title: Tissue-specific Distribution and Dynamic Changes of 5-Hydroxymethylcytosine in Mammalian Genomes *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.217083

    MspI and HpaII isoschizomer can distinguish between 5-hmC and 5-ghmC at the internal cytosine residue. Cleavage specificity is shown for MspI and HpaII on FAM-end-labeled oligonucleotide duplex with internal CG being symmetrically hydroxymethylated or hemihydroxymethylated in the presence or absence of β-GT. Complete cleavage products are labeled as 24 and 19 nt, respectively, on a non-denaturing acrylamide gel for double-hydroxymethylated ( upper panel ) and denaturing acrylamide gel for hemi-hydroxymethylated DNA ( lower panel ). The arrow at the right indicates small amounts of 24-nt-long product.
    Figure Legend Snippet: MspI and HpaII isoschizomer can distinguish between 5-hmC and 5-ghmC at the internal cytosine residue. Cleavage specificity is shown for MspI and HpaII on FAM-end-labeled oligonucleotide duplex with internal CG being symmetrically hydroxymethylated or hemihydroxymethylated in the presence or absence of β-GT. Complete cleavage products are labeled as 24 and 19 nt, respectively, on a non-denaturing acrylamide gel for double-hydroxymethylated ( upper panel ) and denaturing acrylamide gel for hemi-hydroxymethylated DNA ( lower panel ). The arrow at the right indicates small amounts of 24-nt-long product.

    Techniques Used: Labeling, Acrylamide Gel Assay

    Related Articles

    other:

    Article Title: Tissue-specific Distribution and Dynamic Changes of 5-Hydroxymethylcytosine in Mammalian Genomes *
    Article Snippet: Screen for Genomic DNA for Hydroxymethylated Loci Genomic DNA was extracted from E14 ES cells and embryoid bodies using the Qiagen DNeasy Blood and Tissue kit.

    Purification:

    Article Title: Tissue-specific Distribution and Dynamic Changes of 5-Hydroxymethylcytosine in Mammalian Genomes *
    Article Snippet: .. All colonies were picked, grown to 5-ml cultures, purified with Qiagen miniprep kit, and sequenced at the NEB sequencing facility. .. Sequences were then aligned to the appropriate genome with the NCBI Blast software.

    Sequencing:

    Article Title: Tissue-specific Distribution and Dynamic Changes of 5-Hydroxymethylcytosine in Mammalian Genomes *
    Article Snippet: .. All colonies were picked, grown to 5-ml cultures, purified with Qiagen miniprep kit, and sequenced at the NEB sequencing facility. .. Sequences were then aligned to the appropriate genome with the NCBI Blast software.

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  • 86
    Qiagen hydroxymethylated loci genomic dna
    MspI and HpaII isoschizomer can distinguish between 5-hmC and 5-ghmC at the internal cytosine residue. Cleavage specificity is shown for MspI and HpaII on FAM-end-labeled oligonucleotide duplex with internal CG being symmetrically <t>hydroxymethylated</t> or hemihydroxymethylated in the presence or absence of β-GT. Complete cleavage products are labeled as 24 and 19 nt, respectively, on a non-denaturing acrylamide gel for double-hydroxymethylated ( upper panel ) and denaturing acrylamide gel for hemi-hydroxymethylated <t>DNA</t> ( lower panel ). The arrow at the right indicates small amounts of 24-nt-long product.
    Hydroxymethylated Loci Genomic Dna, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hydroxymethylated loci genomic dna/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hydroxymethylated loci genomic dna - by Bioz Stars, 2021-06
    86/100 stars
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    MspI and HpaII isoschizomer can distinguish between 5-hmC and 5-ghmC at the internal cytosine residue. Cleavage specificity is shown for MspI and HpaII on FAM-end-labeled oligonucleotide duplex with internal CG being symmetrically hydroxymethylated or hemihydroxymethylated in the presence or absence of β-GT. Complete cleavage products are labeled as 24 and 19 nt, respectively, on a non-denaturing acrylamide gel for double-hydroxymethylated ( upper panel ) and denaturing acrylamide gel for hemi-hydroxymethylated DNA ( lower panel ). The arrow at the right indicates small amounts of 24-nt-long product.

    Journal: The Journal of Biological Chemistry

    Article Title: Tissue-specific Distribution and Dynamic Changes of 5-Hydroxymethylcytosine in Mammalian Genomes *

    doi: 10.1074/jbc.M110.217083

    Figure Lengend Snippet: MspI and HpaII isoschizomer can distinguish between 5-hmC and 5-ghmC at the internal cytosine residue. Cleavage specificity is shown for MspI and HpaII on FAM-end-labeled oligonucleotide duplex with internal CG being symmetrically hydroxymethylated or hemihydroxymethylated in the presence or absence of β-GT. Complete cleavage products are labeled as 24 and 19 nt, respectively, on a non-denaturing acrylamide gel for double-hydroxymethylated ( upper panel ) and denaturing acrylamide gel for hemi-hydroxymethylated DNA ( lower panel ). The arrow at the right indicates small amounts of 24-nt-long product.

    Article Snippet: Screen for Genomic DNA for Hydroxymethylated Loci Genomic DNA was extracted from E14 ES cells and embryoid bodies using the Qiagen DNeasy Blood and Tissue kit.

    Techniques: Labeling, Acrylamide Gel Assay