Article Title: A Solution to Antifolate Resistance in Group B Streptococcus: Untargeted Metabolomics Identifies Human Milk Oligosaccharide-Induced Perturbations That Result in Potentiation of Trimethoprim
Figure Lengend Snippet: Workflow and pathway analysis using global, untargeted metabolomics data analysis. (A) Overview of global, untargeted metabolomic workflow. (B) Global output of identified metabolites from RPLC and HILIC methods and subsequent filtering for significance according to a P value of ≤0.05 and fold change of ≥|2|. (C) Table output of metabolic pathway enrichment analysis. The number of total metabolites in the pathway, the number of hits, and the P value were calculated using MetaboAnalyst 4.0. CoA, coenzyme A. (D) Metabolomic pathway analysis visualization. Shown is a graphical representation analysis using the statistically significant metabolite compounds ( P ≤ 0.05; fold change, ≥|2|) annotated from RPLC and HILIC analyses. Matched pathways were arranged by P values (from pathway enrichment analysis) on the y axis, and pathway impact values (from pathway topology analysis) are shown on the x axis; node color is based on pathway P value, and node radius is determined based on pathway impact values; individual nodes represent individual pathways.
Article Snippet: Metabolite extracts were analyzed using reverse-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC), followed by subsequent mass spectrometry analysis using a high-resolution Q-Exactive high-fidelity (HF) hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) equipped with a Vanquish ultrahigh-performance liquid chromatography (UHPLC) binary system and autosampler (Thermo Fisher Scientific, Bremen, Germany).
Techniques: Hydrophilic Interaction Liquid Chromatography