Journal: International Journal of Molecular Sciences

Article Title: Synthesis and Antibacterial Evaluation of Ciprofloxacin Congeners with Spirocyclic Amine Periphery

doi: 10.3390/ijms24020954

Figure Lengend Snippet: Antibacterial activity as minimal inhibitory concentration (MIC, µg/mL) of compounds 6a–k as well as ciprofloxacin (positive control) against the ESKAPE panel of pathogens (E1 = Enterobacter cloacae (G−), S = Staphylococcus aureus (G+), K = Klebsiella pneumoniae (G−), A = Acinetobacter baumannii (G−), P = Pseudomonas aeruginosa (G−), and E2 = Enterococcus faecalis (G+)); NT—not tested (the disk diffusion inhibition zone diameter did not warrant MIC determination). The MIC values are mean from three different assays (errors were in the range of ±5–10% of the reported values). Compound 3w from [ 18 ] is provided as a comparison. The antibacterial activity of the lead compound ( 6a ) is underscored.

Article Snippet: Testing was conducted against the following microorganisms: Enterococcus faecalis (ATCC 29812), Staphylococcus aureus (ATCC 25912), Klebsiella pneumoniae (ATCC 19882), Acinetobacter baumannii (948 ® , patient-derived strain from the Pasteur Institute own collection), Pseudomonas aeruginosa (ATCC 27853) and Enterobacter cloacae (ATCC 13047) for compounds 6a–k as well as ciprofloxacin (employed as a positive control) using the Kirby–Bauer disk diffusion test [ ] under the Standard Operating Procedure of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) [ ].

Techniques: Activity Assay, Concentration Assay, Positive Control, Diffusion-based Assay, Inhibition

Journal: Microorganisms

Article Title: Rapid Detection and Quantification of Viable Cells of Pectobacterium brasiliense Using Propidium Monoazide Combined with Real-Time PCR

doi: 10.3390/microorganisms11112808

Figure Lengend Snippet: Detection of the specificity of qPCR assays for Pectobacterium brasiliense using 48 bacterial strains.

Article Snippet: 46 , Acidovorax citrulli , ATCC 29625 , Watermelon , USA , −.

Techniques:

Journal: Microorganisms

Article Title: Circulating Bacterial DNA as Plasma Biomarkers for Lung Cancer Early Detection

doi: 10.3390/microorganisms11030582

Figure Lengend Snippet: DNA abundances of eight bacterial genera in tissues specimens of 58 lung cancer cases.

Article Snippet: Bacteria, including Azoarcus oleivorans (ATCC2411), Aeromicrobium fastidiosum (ATCC12713), Acidovorax avenae (12530), Capnocytophaga canimorsus (ATCC35979), Fusobacterium nucleatum (ATCC25586), Haemophilus influenzae (ATCC51907), Neisseria meningitidis (ATCC13077), Streptococcus pneumoniae (ATCC6303), Selenomonas noxia (ATCC43541), and Veillonella parvula (ATCC 17742), were purchased from American Type Culture Collection (ATCC, Gaithersburg, MD, USA) and cultured as previously described [ , ].

Techniques: Standard Deviation

Journal: bioRxiv

Article Title: Acidovorax temperans polarizes T17 cells and skews neutrophil maturation to promote lung adenocarcinoma development

doi: 10.1101/2022.12.20.521215

Figure Lengend Snippet: (A) Percent of cells Il17a positive per T cell subtype and treatment group. (B and C) Expression of a pan T17 cell gene signature in Il17a negative (absent) and positive (expressed) cells within the Th17 (B) and γδ T (C) clusters. (D and E) Expression of a pan T17 cell gene signature in all cells within each T cell subtype (D) and by treatment group (E). (F) Correlation of Acidovorax abundance (genus level) and the murine pan T17 gene signature expression within the TCGA LUAD dataset . (G) Kaplan-Meier curves for survival property within four human LUAD cohorts – GSE30219, GSE31210, GSE50081, and TCGA ( ; ; ; ).

Article Snippet: Acidovorax temperans ATCC 49666 was obtained from the ATCC (Manassas, VA) and cultured in Nutrient Broth (BD Biosciences) at 30°C with 200 rpm shaking.

Techniques: Expressing