hybrid ltq orbitrap elite mass spectrometer  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher hybrid ltq orbitrap elite mass spectrometer
    Hybrid Ltq Orbitrap Elite Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid ltq orbitrap elite mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hybrid ltq orbitrap elite mass spectrometer - by Bioz Stars, 2020-03
    99/100 stars

    Images

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Proteolytic Surface-Shaving and Serotype-Dependent Expression of SPI-1 Invasion Proteins in Salmonella enterica Subspecies enterica
    Article Snippet: Samples were analyzed using a nano-LC system (Tempo™, nano MDLC, Applied Biosystems/Eksigent) with a PicoSlide nano-electrospray (nano-ESI, 3 column set-up) ion source (New Objective, Woburn, MA) coupled to a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, San Jose, CA). .. The sample slug was then transferred to a one of the three PicoChip columns (C18-AQ, 3 μm, 120 Å, 105 mm, New Objective) at a flow rate of 400 nL/min using a NanoEasy n-LC II (Thermo Scientific) HPLC.

    Flow Cytometry:

    Article Title: Predegenerated Schwann cells–a novel prospect for cell therapy for glaucoma: neuroprotection, neuroregeneration and neuroplasticity
    Article Snippet: The trypsinized samples were purified with C18 silica microspin columns (The Nest Group, Southborough, MA, USA) and analyzed on a hybrid LTQ Orbitrap Elite Mass Spectrometer (Thermo Scientific, Rockford, IL, USA) coupled to an Easy nLCII (liquid chromatography) nanoflow system (Thermo Scientific, Rockford, IL, USA) via a nanoelectrospray ion source (Thermo Scientific, Rockford, IL, USA). .. Peptides were eluted the analytical column with a gradient of 60 min of buffer B ranging from 5% to 35% at a constant flow rate of 300 nl/min followed by a 10 min gradient from 35% to 80% of solvent B.

    Article Title: Proteolytic Surface-Shaving and Serotype-Dependent Expression of SPI-1 Invasion Proteins in Salmonella enterica Subspecies enterica
    Article Snippet: Samples were analyzed using a nano-LC system (Tempo™, nano MDLC, Applied Biosystems/Eksigent) with a PicoSlide nano-electrospray (nano-ESI, 3 column set-up) ion source (New Objective, Woburn, MA) coupled to a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, San Jose, CA). .. The sample slug was then transferred to a one of the three PicoChip columns (C18-AQ, 3 μm, 120 Å, 105 mm, New Objective) at a flow rate of 400 nL/min using a NanoEasy n-LC II (Thermo Scientific) HPLC.

    Article Title: High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies
    Article Snippet: Samples were injected via auto-sampler to a BEH µC18 column (Waters Corp) at a flow rate of 1 µL/min using a NanoAcquity UPLC (Waters Corp). .. Samples were analyzed on-line via nanospray ionization into a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher Scientific).

    Article Title: Molecular Imaging of Endometriosis Tissues using Desorption Electrospray Ionization Mass Spectrometry
    Article Snippet: .. DESI-MS imaging was performed in the negative ion mode from m/z 100–1500, using a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Scientific, San Jose, CA) at 60,000 resolving power (at m/z 200) using dimethylformamide:acetonitrile 1:3 (v/v) at a flow rate of 1.4 µL/min unless otherwise stated. ..

    Liquid Chromatography:

    Article Title: Predegenerated Schwann cells–a novel prospect for cell therapy for glaucoma: neuroprotection, neuroregeneration and neuroplasticity
    Article Snippet: .. The trypsinized samples were purified with C18 silica microspin columns (The Nest Group, Southborough, MA, USA) and analyzed on a hybrid LTQ Orbitrap Elite Mass Spectrometer (Thermo Scientific, Rockford, IL, USA) coupled to an Easy nLCII (liquid chromatography) nanoflow system (Thermo Scientific, Rockford, IL, USA) via a nanoelectrospray ion source (Thermo Scientific, Rockford, IL, USA). .. The following solvents were used for liquid chromatography separation of the digested samples: solvent A (0.1% formic acid, 98% water, 2% acetonitrile), and solvent B (0.1% formic acid, 98% acetonitrile, 2% water).

    Sample Prep:

    Article Title: Induction of the Immunoproteasome Subunit Lmp7 Links Proteostasis and Immunity in α-Synuclein Aggregation Disorders
    Article Snippet: Paragraph title: Sample Preparation and LC-MS/MS Analysis ... Peptide digests were analyzed on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled with a NanoLC Ultra (Eksigent Technologies).

    Chromatography:

    Article Title: Proteolytic Surface-Shaving and Serotype-Dependent Expression of SPI-1 Invasion Proteins in Salmonella enterica Subspecies enterica
    Article Snippet: Paragraph title: Nano-Liquid Chromatography-Tandem Mass Spectrometry (nano-LC-MS/MS) ... Samples were analyzed using a nano-LC system (Tempo™, nano MDLC, Applied Biosystems/Eksigent) with a PicoSlide nano-electrospray (nano-ESI, 3 column set-up) ion source (New Objective, Woburn, MA) coupled to a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, San Jose, CA).

    Article Title: Induction of the Immunoproteasome Subunit Lmp7 Links Proteostasis and Immunity in α-Synuclein Aggregation Disorders
    Article Snippet: Peptide concentration was determined by UV280 before they were separated by high-pH reverse phage chromatography (Acquity UPLC H-Class instrument, Waters) to 72 fractions. .. Peptide digests were analyzed on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled with a NanoLC Ultra (Eksigent Technologies).

    Article Title: Unbiased Proteomics of Early Lewy Body Formation Model Implicates Active Microtubule Affinity-Regulating Kinases (MARKs) in Synucleinopathies
    Article Snippet: The 2% SDS-soluble fractions of PBS- or α-synuclein PFF-treated neurons were separated on a 10% Bis-tris gel and subjected to tandem liquid chromatography-mass spectrometry (LC-MS/MS) analysis as described previously ( ). .. Tryptic digests were analyzed by LC-MS/MS on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermo Fisher Scientific) coupled with a nanoLC Ultra (Eksigent).

    Positive Control:

    Article Title: Predegenerated Schwann cells–a novel prospect for cell therapy for glaucoma: neuroprotection, neuroregeneration and neuroplasticity
    Article Snippet: The trypsinized samples were purified with C18 silica microspin columns (The Nest Group, Southborough, MA, USA) and analyzed on a hybrid LTQ Orbitrap Elite Mass Spectrometer (Thermo Scientific, Rockford, IL, USA) coupled to an Easy nLCII (liquid chromatography) nanoflow system (Thermo Scientific, Rockford, IL, USA) via a nanoelectrospray ion source (Thermo Scientific, Rockford, IL, USA). .. As a positive control for the MS analysis, a solution of 1.0 and 0.1 ng/ml human brain derived neurotrophic factor (BDNF, PeproTech EC Ltd, London, UK) and 1.0 and 0.1 ng/ml rat ciliary neurotrophic factor (CNTF, PeproTech EC Ltd, London, UK) in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12- (DMEM-F12, Sigma, St. Louis, MO, USA) was used.

    Isolation:

    Article Title: Loss of PIKfyve in platelets causes a lysosomal disease leading to inflammation and thrombosis in mice
    Article Snippet: Activated platelets were centrifuged at 13,000g for 10 minutes at 4°C and the supernates were isolated from the pellets. .. Tryptic digests were analyzed by LC-MS/MS on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermofisher Scientific San Jose, CA) coupled with a nano LC Ultra (Eksigent).

    BIA-KA:

    Article Title: Loss of PIKfyve in platelets causes a lysosomal disease leading to inflammation and thrombosis in mice
    Article Snippet: The concentrations of proteins from the platelet supernates were measured using the BCA protein assay (Pierce). .. Tryptic digests were analyzed by LC-MS/MS on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermofisher Scientific San Jose, CA) coupled with a nano LC Ultra (Eksigent).

    Blocking Assay:

    Article Title: Predegenerated Schwann cells–a novel prospect for cell therapy for glaucoma: neuroprotection, neuroregeneration and neuroplasticity
    Article Snippet: Iodoacetamide 100 mM (Sigma, St. Louis, MO, USA) was used for blocking cysteine residues at RT in the dark for 30 minutes; thereafter, the samples were incubated with trypsin (Promega V5113, Madison,WI, USA) with an enzyme to substrate ratio of 1:20 (w/w) at 37 °C overnight. .. The trypsinized samples were purified with C18 silica microspin columns (The Nest Group, Southborough, MA, USA) and analyzed on a hybrid LTQ Orbitrap Elite Mass Spectrometer (Thermo Scientific, Rockford, IL, USA) coupled to an Easy nLCII (liquid chromatography) nanoflow system (Thermo Scientific, Rockford, IL, USA) via a nanoelectrospray ion source (Thermo Scientific, Rockford, IL, USA).

    Purification:

    Article Title: Predegenerated Schwann cells–a novel prospect for cell therapy for glaucoma: neuroprotection, neuroregeneration and neuroplasticity
    Article Snippet: .. The trypsinized samples were purified with C18 silica microspin columns (The Nest Group, Southborough, MA, USA) and analyzed on a hybrid LTQ Orbitrap Elite Mass Spectrometer (Thermo Scientific, Rockford, IL, USA) coupled to an Easy nLCII (liquid chromatography) nanoflow system (Thermo Scientific, Rockford, IL, USA) via a nanoelectrospray ion source (Thermo Scientific, Rockford, IL, USA). .. The following solvents were used for liquid chromatography separation of the digested samples: solvent A (0.1% formic acid, 98% water, 2% acetonitrile), and solvent B (0.1% formic acid, 98% acetonitrile, 2% water).

    Article Title: High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies
    Article Snippet: Glycosylation site occupancy determination 40 µg purified WT MICA, MICA GEM variants, WT HER2 or HER GEM variants were reduced with 10 mM dithiothreitol (37°C for one hour, Sigma, St. Louis, MO), alkylated with 20 mM iodoacetic acid (Sigma, 20 minutes at ambient temperature), deglycosylated with PNGaseF (Sigma, 1U, 37°C, 18 hours) then digested with either trypsin, chymotrypsin, glutamyl endopeptidase from S. aureus , or Aspariginyl peptidase from P. fragi in 50 mM ammonium bicarbonate pH 8, overnight at 37°C (0.2 µg or 2 µg enzyme, Roche). .. Samples were analyzed on-line via nanospray ionization into a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher Scientific).

    Article Title: Nε-fatty acylation of multiple membrane-associated proteins by Shigella IcsB effector to modulate host function
    Article Snippet: To identify the exact lipid modification, the above purified RhoA was subjected to trypsin digestion as described previously . .. Peptides eluted from the capillary column were applied directly onto a hybrid LTQ-Orbitrap Elite mass spectrometer by electrospray (Thermo Fisher Scientific) for mass spectrometry (MS) and MS/MS analyses.

    Concentration Assay:

    Article Title: Predegenerated Schwann cells–a novel prospect for cell therapy for glaucoma: neuroprotection, neuroregeneration and neuroplasticity
    Article Snippet: Tris (2-carboxyethyl) phosphine hydrochloride at a final concentration of 5 mM (Sigma, St. Louis, MO, USA) was used for the reduction of disulfide bonds at 37 °C for 30 minutes. .. The trypsinized samples were purified with C18 silica microspin columns (The Nest Group, Southborough, MA, USA) and analyzed on a hybrid LTQ Orbitrap Elite Mass Spectrometer (Thermo Scientific, Rockford, IL, USA) coupled to an Easy nLCII (liquid chromatography) nanoflow system (Thermo Scientific, Rockford, IL, USA) via a nanoelectrospray ion source (Thermo Scientific, Rockford, IL, USA).

    Article Title: Induction of the Immunoproteasome Subunit Lmp7 Links Proteostasis and Immunity in α-Synuclein Aggregation Disorders
    Article Snippet: Peptide concentration was determined by UV280 before they were separated by high-pH reverse phage chromatography (Acquity UPLC H-Class instrument, Waters) to 72 fractions. .. Peptide digests were analyzed on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled with a NanoLC Ultra (Eksigent Technologies).

    Incubation:

    Article Title: Predegenerated Schwann cells–a novel prospect for cell therapy for glaucoma: neuroprotection, neuroregeneration and neuroplasticity
    Article Snippet: Iodoacetamide 100 mM (Sigma, St. Louis, MO, USA) was used for blocking cysteine residues at RT in the dark for 30 minutes; thereafter, the samples were incubated with trypsin (Promega V5113, Madison,WI, USA) with an enzyme to substrate ratio of 1:20 (w/w) at 37 °C overnight. .. The trypsinized samples were purified with C18 silica microspin columns (The Nest Group, Southborough, MA, USA) and analyzed on a hybrid LTQ Orbitrap Elite Mass Spectrometer (Thermo Scientific, Rockford, IL, USA) coupled to an Easy nLCII (liquid chromatography) nanoflow system (Thermo Scientific, Rockford, IL, USA) via a nanoelectrospray ion source (Thermo Scientific, Rockford, IL, USA).

    Imaging:

    Article Title: Cardiolipins are biomarkers of mitochondria-rich thyroid oncocytic tumors
    Article Snippet: .. DESI-MS imaging was performed in the negative ion mode from m/z 100–1500, using a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Scientific, San Jose, CA). ..

    Article Title: Molecular Imaging of Endometriosis Tissues using Desorption Electrospray Ionization Mass Spectrometry
    Article Snippet: .. DESI-MS imaging was performed in the negative ion mode from m/z 100–1500, using a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Scientific, San Jose, CA) at 60,000 resolving power (at m/z 200) using dimethylformamide:acetonitrile 1:3 (v/v) at a flow rate of 1.4 µL/min unless otherwise stated. ..

    Article Title: Detection of Metastatic Breast and Thyroid Cancer in Lymph Nodes by Desorption Electrospray Ionization Mass Spectrometry Imaging
    Article Snippet: Paragraph title: DESI-MS imaging ... DESI-MSI was performed in the negative ion mode from m/z 100-1500, using a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Scientific, San Jose, CA).

    Mass Spectrometry:

    Article Title: Predegenerated Schwann cells–a novel prospect for cell therapy for glaucoma: neuroprotection, neuroregeneration and neuroplasticity
    Article Snippet: .. The trypsinized samples were purified with C18 silica microspin columns (The Nest Group, Southborough, MA, USA) and analyzed on a hybrid LTQ Orbitrap Elite Mass Spectrometer (Thermo Scientific, Rockford, IL, USA) coupled to an Easy nLCII (liquid chromatography) nanoflow system (Thermo Scientific, Rockford, IL, USA) via a nanoelectrospray ion source (Thermo Scientific, Rockford, IL, USA). .. The following solvents were used for liquid chromatography separation of the digested samples: solvent A (0.1% formic acid, 98% water, 2% acetonitrile), and solvent B (0.1% formic acid, 98% acetonitrile, 2% water).

    Article Title: Proteomic Analyses of Intracellular Salmonella enterica Serovar Typhimurium Reveal Extensive Bacterial Adaptations to Infected Host Epithelial Cells
    Article Snippet: .. A hybrid LTQ-Orbitrap Elite mass spectrometer (equipped with an EASY-nLC 1000 system; Thermo Scientific) was used for proteomic and metabolomic analyses. .. The capillary column (75 μm by 150 mm) with a laser-pulled electrospray tip (model P-2000; Sutter Instruments) was home-packed with 4-μm-diameter, 100-Å Magic C18AQ silica-based particles (Michrom BioResources Inc., Auburn, CA).

    Article Title: Ambient Ionization and FAIMS Mass Spectrometry for Enhanced Imaging of Multiply Charged Molecular Ions in Biological Tissues
    Article Snippet: .. Protein species were analyzed by top-down collision induced dissociation (CID) tandem MS, performed on a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Scientific, San Jose, CA) at 120 000 resolving power at m/z ) and compared with protein amino acid sequences that have been previously observed within the tissue types analyzed. ..

    Article Title: Proteolytic Surface-Shaving and Serotype-Dependent Expression of SPI-1 Invasion Proteins in Salmonella enterica Subspecies enterica
    Article Snippet: .. Samples were analyzed using a nano-LC system (Tempo™, nano MDLC, Applied Biosystems/Eksigent) with a PicoSlide nano-electrospray (nano-ESI, 3 column set-up) ion source (New Objective, Woburn, MA) coupled to a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, San Jose, CA). ..

    Article Title: Ndfip-mediated degradation of Jak1 tunes cytokine signalling to limit expansion of CD4+ effector T cells
    Article Snippet: .. LC-MS/MS Tryptic digests were analysed by LC-MS/MS on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermofisher Scientific) coupled with a nanoLC Ultra (Eksigent). .. Peptides were separated by reverse phase (RP)-HPLC on a nanocapillary column, 75 μM ID × 15 cm Reprosil-pur 3 μM (Dr Maisch, Germany) in a Nanoflex chip system (Eksigent).

    Article Title: Induction of the Immunoproteasome Subunit Lmp7 Links Proteostasis and Immunity in α-Synuclein Aggregation Disorders
    Article Snippet: .. Peptide digests were analyzed on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled with a NanoLC Ultra (Eksigent Technologies). ..

    Article Title: Loss of PIKfyve in platelets causes a lysosomal disease leading to inflammation and thrombosis in mice
    Article Snippet: .. Tryptic digests were analyzed by LC-MS/MS on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermofisher Scientific San Jose, CA) coupled with a nano LC Ultra (Eksigent). .. Peptides were separated by reverse phase (RP)-HPLC on a nanocapillary column, 75 µm id × 15 cm Reprosil-pur 3uM (Dr. Maisch, Germany) in a Nanoflex chip system (Eksigent).

    Article Title: The Kringle-like Domain Facilitates Post-endoplasmic Reticulum Changes to Premelanosome Protein (PMEL) Oligomerization and Disulfide Bond Configuration and Promotes Amyloid Formation *
    Article Snippet: .. Tryptic digests were analyzed by LC-MS/MS on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermo Fisher Scientific) coupled with a nanoLC Ultra (Eksigent Technologies Inc., Dublin, CA). .. Peptides were separated by reverse phase-HPLC on a nanocapillary column (75 μm × 15 cm Reprosil-pur 3 μm, 120 Å (Dr. Maisch, Ammerbuch, Germany)) in a Nanoflex chip system (Eksigent Technologies).

    Article Title: Cardiolipins are biomarkers of mitochondria-rich thyroid oncocytic tumors
    Article Snippet: .. DESI-MS imaging was performed in the negative ion mode from m/z 100–1500, using a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Scientific, San Jose, CA). ..

    Article Title: High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies
    Article Snippet: .. Samples were analyzed on-line via nanospray ionization into a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher Scientific). ..

    Article Title: Nε-fatty acylation of multiple membrane-associated proteins by Shigella IcsB effector to modulate host function
    Article Snippet: .. Peptides eluted from the capillary column were applied directly onto a hybrid LTQ-Orbitrap Elite mass spectrometer by electrospray (Thermo Fisher Scientific) for mass spectrometry (MS) and MS/MS analyses. ..

    Article Title: Unbiased Proteomics of Early Lewy Body Formation Model Implicates Active Microtubule Affinity-Regulating Kinases (MARKs) in Synucleinopathies
    Article Snippet: .. Tryptic digests were analyzed by LC-MS/MS on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermo Fisher Scientific) coupled with a nanoLC Ultra (Eksigent). ..

    Article Title: Calpain-mediated tau fragmentation is altered in Alzheimer’s disease progression
    Article Snippet: .. Samples were analyzed via nanospray ionization into a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher). ..

    Article Title: Molecular Imaging of Endometriosis Tissues using Desorption Electrospray Ionization Mass Spectrometry
    Article Snippet: .. DESI-MS imaging was performed in the negative ion mode from m/z 100–1500, using a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Scientific, San Jose, CA) at 60,000 resolving power (at m/z 200) using dimethylformamide:acetonitrile 1:3 (v/v) at a flow rate of 1.4 µL/min unless otherwise stated. ..

    Article Title: Detection of Metastatic Breast and Thyroid Cancer in Lymph Nodes by Desorption Electrospray Ionization Mass Spectrometry Imaging
    Article Snippet: .. DESI-MSI was performed in the negative ion mode from m/z 100-1500, using a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Scientific, San Jose, CA). ..

    Tandem Mass Spectroscopy:

    Article Title: Nε-fatty acylation of multiple membrane-associated proteins by Shigella IcsB effector to modulate host function
    Article Snippet: .. Peptides eluted from the capillary column were applied directly onto a hybrid LTQ-Orbitrap Elite mass spectrometer by electrospray (Thermo Fisher Scientific) for mass spectrometry (MS) and MS/MS analyses. ..

    Article Title: Calpain-mediated tau fragmentation is altered in Alzheimer’s disease progression
    Article Snippet: Samples were analyzed via nanospray ionization into a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher). .. MS/MS data was analyzed using the Mascot algorithm (Matrix Sciences).

    Modification:

    Article Title: Predegenerated Schwann cells–a novel prospect for cell therapy for glaucoma: neuroprotection, neuroregeneration and neuroplasticity
    Article Snippet: The trypsinized samples were purified with C18 silica microspin columns (The Nest Group, Southborough, MA, USA) and analyzed on a hybrid LTQ Orbitrap Elite Mass Spectrometer (Thermo Scientific, Rockford, IL, USA) coupled to an Easy nLCII (liquid chromatography) nanoflow system (Thermo Scientific, Rockford, IL, USA) via a nanoelectrospray ion source (Thermo Scientific, Rockford, IL, USA). .. As a positive control for the MS analysis, a solution of 1.0 and 0.1 ng/ml human brain derived neurotrophic factor (BDNF, PeproTech EC Ltd, London, UK) and 1.0 and 0.1 ng/ml rat ciliary neurotrophic factor (CNTF, PeproTech EC Ltd, London, UK) in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12- (DMEM-F12, Sigma, St. Louis, MO, USA) was used.

    Article Title: Nε-fatty acylation of multiple membrane-associated proteins by Shigella IcsB effector to modulate host function
    Article Snippet: Paragraph title: Mass spectrometry analyses of RhoA modification by IcsB. ... Peptides eluted from the capillary column were applied directly onto a hybrid LTQ-Orbitrap Elite mass spectrometer by electrospray (Thermo Fisher Scientific) for mass spectrometry (MS) and MS/MS analyses.

    Injection:

    Article Title: High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies
    Article Snippet: Samples were injected via auto-sampler to a BEH µC18 column (Waters Corp) at a flow rate of 1 µL/min using a NanoAcquity UPLC (Waters Corp). .. Samples were analyzed on-line via nanospray ionization into a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher Scientific).

    Article Title: Calpain-mediated tau fragmentation is altered in Alzheimer’s disease progression
    Article Snippet: Pooled extractions were reconstituted in 2% acetonitrile: 0.1% formic acid and injected onto a 75 µm × 100 mm column (BEH, 1.7 micron, Waters Corp) using a NanoAcquity UPLC (Waters Corp). .. Samples were analyzed via nanospray ionization into a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher).

    Chromatin Immunoprecipitation:

    Article Title: Ndfip-mediated degradation of Jak1 tunes cytokine signalling to limit expansion of CD4+ effector T cells
    Article Snippet: LC-MS/MS Tryptic digests were analysed by LC-MS/MS on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermofisher Scientific) coupled with a nanoLC Ultra (Eksigent). .. Peptides were separated by reverse phase (RP)-HPLC on a nanocapillary column, 75 μM ID × 15 cm Reprosil-pur 3 μM (Dr Maisch, Germany) in a Nanoflex chip system (Eksigent).

    Article Title: Loss of PIKfyve in platelets causes a lysosomal disease leading to inflammation and thrombosis in mice
    Article Snippet: Tryptic digests were analyzed by LC-MS/MS on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermofisher Scientific San Jose, CA) coupled with a nano LC Ultra (Eksigent). .. Peptides were separated by reverse phase (RP)-HPLC on a nanocapillary column, 75 µm id × 15 cm Reprosil-pur 3uM (Dr. Maisch, Germany) in a Nanoflex chip system (Eksigent).

    Article Title: The Kringle-like Domain Facilitates Post-endoplasmic Reticulum Changes to Premelanosome Protein (PMEL) Oligomerization and Disulfide Bond Configuration and Promotes Amyloid Formation *
    Article Snippet: Tryptic digests were analyzed by LC-MS/MS on a hybrid LTQ Orbitrap Elite mass spectrometer (Thermo Fisher Scientific) coupled with a nanoLC Ultra (Eksigent Technologies Inc., Dublin, CA). .. Peptides were separated by reverse phase-HPLC on a nanocapillary column (75 μm × 15 cm Reprosil-pur 3 μm, 120 Å (Dr. Maisch, Ammerbuch, Germany)) in a Nanoflex chip system (Eksigent Technologies).

    SDS Page:

    Article Title: Calpain-mediated tau fragmentation is altered in Alzheimer’s disease progression
    Article Snippet: SDS-PAGE bands were excised, washed in 50 mM ammonium bicarbonate in 50∶50 acetonitrile:water for 20 min, dehydrated with acetonitrile and trypsin-digested overnight (Promega) at 37 °C. .. Samples were analyzed via nanospray ionization into a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher).

    Derivative Assay:

    Article Title: Predegenerated Schwann cells–a novel prospect for cell therapy for glaucoma: neuroprotection, neuroregeneration and neuroplasticity
    Article Snippet: The trypsinized samples were purified with C18 silica microspin columns (The Nest Group, Southborough, MA, USA) and analyzed on a hybrid LTQ Orbitrap Elite Mass Spectrometer (Thermo Scientific, Rockford, IL, USA) coupled to an Easy nLCII (liquid chromatography) nanoflow system (Thermo Scientific, Rockford, IL, USA) via a nanoelectrospray ion source (Thermo Scientific, Rockford, IL, USA). .. As a positive control for the MS analysis, a solution of 1.0 and 0.1 ng/ml human brain derived neurotrophic factor (BDNF, PeproTech EC Ltd, London, UK) and 1.0 and 0.1 ng/ml rat ciliary neurotrophic factor (CNTF, PeproTech EC Ltd, London, UK) in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12- (DMEM-F12, Sigma, St. Louis, MO, USA) was used.

    Software:

    Article Title: Ambient Ionization and FAIMS Mass Spectrometry for Enhanced Imaging of Multiply Charged Molecular Ions in Biological Tissues
    Article Snippet: The data was deconvoluted using the Xtract function within Xcalibur software using a S/N threshold of 3 to obtain monoisotopic masses of protein species detected during experiments. .. Protein species were analyzed by top-down collision induced dissociation (CID) tandem MS, performed on a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Scientific, San Jose, CA) at 120 000 resolving power at m/z ) and compared with protein amino acid sequences that have been previously observed within the tissue types analyzed.

    Article Title: High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies
    Article Snippet: Samples were analyzed on-line via nanospray ionization into a hybrid LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher Scientific). .. Byologic software (Protein Metrics) was used to quantify sites of N-linked glycosylation as evidenced by the presence of asparaginyl deamidation.

    Article Title: Nε-fatty acylation of multiple membrane-associated proteins by Shigella IcsB effector to modulate host function
    Article Snippet: The protein charge envelope was averaged across the corresponding protein elution peaks and deconvoluted into the uncharged form using the BioAnalyst software provided by the manufacturer. .. Peptides eluted from the capillary column were applied directly onto a hybrid LTQ-Orbitrap Elite mass spectrometer by electrospray (Thermo Fisher Scientific) for mass spectrometry (MS) and MS/MS analyses.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher hybrid ion trap orbitrap mass spectrometer
    (a) Optical image of an H E-stained longitudinal histological section of retrocerebral complex of P. americana , showing distinct neuroendocrine regions and surrounding tissues. Abbreviations: CA, corpus allatum; CCg, glandular lobe of corpus cardiacum; CCs, storage lobe of corpus cardiacum; fg, foregut epithelium and visceral muscle tissue; rn, recurrent nerve; tr, trachea. Scale bar: 0.75 mm. Positive-ion mode high mass resolution <t>Orbitrap</t> MALDI-MS spectra acquired from a single 20 μm pixel after background subtraction within (b) CCs and (c) CCg regions.
    Hybrid Ion Trap Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid ion trap orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 150 article reviews
    Price from $9.99 to $1999.99
    hybrid ion trap orbitrap mass spectrometer - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher ltq velos orbitrap elite hybrid mass spectrometer
    (a) Optical image of an H E-stained longitudinal histological section of retrocerebral complex of P. americana , showing distinct neuroendocrine regions and surrounding tissues. Abbreviations: CA, corpus allatum; CCg, glandular lobe of corpus cardiacum; CCs, storage lobe of corpus cardiacum; fg, foregut epithelium and visceral muscle tissue; rn, recurrent nerve; tr, trachea. Scale bar: 0.75 mm. Positive-ion mode high mass resolution <t>Orbitrap</t> MALDI-MS spectra acquired from a single 20 μm pixel after background subtraction within (b) CCs and (c) CCg regions.
    Ltq Velos Orbitrap Elite Hybrid Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq velos orbitrap elite hybrid mass spectrometer/product/Thermo Fisher
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ltq velos orbitrap elite hybrid mass spectrometer - by Bioz Stars, 2020-03
    92/100 stars
      Buy from Supplier

    99
    Thermo Fisher ltq orbitrap elite hybrid mass spectrometer
    (a) Optical image of an H E-stained longitudinal histological section of retrocerebral complex of P. americana , showing distinct neuroendocrine regions and surrounding tissues. Abbreviations: CA, corpus allatum; CCg, glandular lobe of corpus cardiacum; CCs, storage lobe of corpus cardiacum; fg, foregut epithelium and visceral muscle tissue; rn, recurrent nerve; tr, trachea. Scale bar: 0.75 mm. Positive-ion mode high mass resolution <t>Orbitrap</t> MALDI-MS spectra acquired from a single 20 μm pixel after background subtraction within (b) CCs and (c) CCg regions.
    Ltq Orbitrap Elite Hybrid Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap elite hybrid mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap elite hybrid mass spectrometer - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    (a) Optical image of an H E-stained longitudinal histological section of retrocerebral complex of P. americana , showing distinct neuroendocrine regions and surrounding tissues. Abbreviations: CA, corpus allatum; CCg, glandular lobe of corpus cardiacum; CCs, storage lobe of corpus cardiacum; fg, foregut epithelium and visceral muscle tissue; rn, recurrent nerve; tr, trachea. Scale bar: 0.75 mm. Positive-ion mode high mass resolution Orbitrap MALDI-MS spectra acquired from a single 20 μm pixel after background subtraction within (b) CCs and (c) CCg regions.

    Journal: Analytical Chemistry

    Article Title: Digestion-Free Analysis of Peptides from 30-year-old Formalin-Fixed, Paraffin-Embedded Tissue by Mass Spectrometry Imaging

    doi: 10.1021/acs.analchem.8b01838

    Figure Lengend Snippet: (a) Optical image of an H E-stained longitudinal histological section of retrocerebral complex of P. americana , showing distinct neuroendocrine regions and surrounding tissues. Abbreviations: CA, corpus allatum; CCg, glandular lobe of corpus cardiacum; CCs, storage lobe of corpus cardiacum; fg, foregut epithelium and visceral muscle tissue; rn, recurrent nerve; tr, trachea. Scale bar: 0.75 mm. Positive-ion mode high mass resolution Orbitrap MALDI-MS spectra acquired from a single 20 μm pixel after background subtraction within (b) CCs and (c) CCg regions.

    Article Snippet: MALDI MS Imaging Acquisition Accurate mass measurements were performed on five replicate samples in positive-ion mode in the range of m /z 500–2000 using a hybrid ion trap-Orbitrap mass spectrometer (LTQ Velos Pro Orbitrap Elite, Thermo Fisher Scientific, Bremen, Germany) coupled to an intermediate-pressure MALDI/ESI source based on a dual ion-funnel geometry (Spectroglyph LLC, Kennewick, WA, U.S.A.).

    Techniques: Staining, Mass Spectrometry

    (a) Correlation clustering of principal components 1–6 of a positive-ion mode MALDI-MSI Orbitrap data set of P. americana FFPE retrocerebral complex tissue section. (b) Mean spectrum representing selected cluster from (a) showing highest spatial correlation with tissue region based on visual comparison. Insets: associated PCA cluster image showing spatial correlation of spectrum at each pixel position, and magnified subset of spectrum for m / z 1100–2000 range. (c) Subset PCA cluster images showing localized distributions for general “on-tissue” features and three specific neuroendocrine tissue regions: CA, CCg, and CCs.

    Journal: Analytical Chemistry

    Article Title: Digestion-Free Analysis of Peptides from 30-year-old Formalin-Fixed, Paraffin-Embedded Tissue by Mass Spectrometry Imaging

    doi: 10.1021/acs.analchem.8b01838

    Figure Lengend Snippet: (a) Correlation clustering of principal components 1–6 of a positive-ion mode MALDI-MSI Orbitrap data set of P. americana FFPE retrocerebral complex tissue section. (b) Mean spectrum representing selected cluster from (a) showing highest spatial correlation with tissue region based on visual comparison. Insets: associated PCA cluster image showing spatial correlation of spectrum at each pixel position, and magnified subset of spectrum for m / z 1100–2000 range. (c) Subset PCA cluster images showing localized distributions for general “on-tissue” features and three specific neuroendocrine tissue regions: CA, CCg, and CCs.

    Article Snippet: MALDI MS Imaging Acquisition Accurate mass measurements were performed on five replicate samples in positive-ion mode in the range of m /z 500–2000 using a hybrid ion trap-Orbitrap mass spectrometer (LTQ Velos Pro Orbitrap Elite, Thermo Fisher Scientific, Bremen, Germany) coupled to an intermediate-pressure MALDI/ESI source based on a dual ion-funnel geometry (Spectroglyph LLC, Kennewick, WA, U.S.A.).

    Techniques: Formalin-fixed Paraffin-Embedded

    (A) The observed chromatographic peak retaining all iron-bound siderophore fragments; (B) graph using a smaller scale of the [M + H] + results, showing the Fe-isotopic pattern observed; (C) precursor mass spectrum collected in the orbitrap

    Journal: Applied and Environmental Microbiology

    Article Title: Acquisition of Iron by Alkaliphilic Bacillus Species ▿

    doi: 10.1128/AEM.01393-10

    Figure Lengend Snippet: (A) The observed chromatographic peak retaining all iron-bound siderophore fragments; (B) graph using a smaller scale of the [M + H] + results, showing the Fe-isotopic pattern observed; (C) precursor mass spectrum collected in the orbitrap

    Article Snippet: Final chromatographic separations and mass spectrometry analyses were performed on a NanoAcquity HPLC system (Waters Corp., Milford, MA) attached to a hybrid linear ion trap-orbitrap mass spectrometer (LTQ-OT; ThermoFisher, San Jose, CA).

    Techniques: