hybrid linear quadrupole ion trap orbitrap mass spectrometer  (Thermo Fisher)


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    LTQ Orbitrap XL Hybrid Ion Trap Orbitrap Mass Spectrometer
    Description:
    Perform a range of applications from protein identification to biomarker discovery de novo sequencing and metabolite profiling with confidence The Thermo Scientific LTQ Orbitrap XL Hybrid Ion Trap Orbitrap Mass Spectrometer is a Fourier Transform Mass Spectrometer FTMS based on Thermo Scientific LTQ XL linear ion trap and Orbitrap mass spectrometer technologies To enhance the flexibility of fragmentation experiments for advanced proteomics and small molecule research the instrument features an HCD collision cell
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    iqlaaegaapfadbmaok
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    Applications:
    Industrial & Applied Science|Industrial Mass Spectrometry
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    Structured Review

    Thermo Fisher hybrid linear quadrupole ion trap orbitrap mass spectrometer
    (a) CID on the <t>LTQ-Orbitrap</t> of the crosslinked homodimer synthetic peptide (Ac-QIGKGVAR) results in the same two major cleavage events observed in MALDI at the intrinsic positive charges. (b) MS 3 of the fragment without an intrinsic positive charge produces
    Perform a range of applications from protein identification to biomarker discovery de novo sequencing and metabolite profiling with confidence The Thermo Scientific LTQ Orbitrap XL Hybrid Ion Trap Orbitrap Mass Spectrometer is a Fourier Transform Mass Spectrometer FTMS based on Thermo Scientific LTQ XL linear ion trap and Orbitrap mass spectrometer technologies To enhance the flexibility of fragmentation experiments for advanced proteomics and small molecule research the instrument features an HCD collision cell
    https://www.bioz.com/result/hybrid linear quadrupole ion trap orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    hybrid linear quadrupole ion trap orbitrap mass spectrometer - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Quaternary Diamines as Mass Spectrometry Cleavable Crosslinkers for Protein Interactions"

    Article Title: Quaternary Diamines as Mass Spectrometry Cleavable Crosslinkers for Protein Interactions

    Journal: Journal of the American Society for Mass Spectrometry

    doi: 10.1007/s13361-011-0288-4

    (a) CID on the LTQ-Orbitrap of the crosslinked homodimer synthetic peptide (Ac-QIGKGVAR) results in the same two major cleavage events observed in MALDI at the intrinsic positive charges. (b) MS 3 of the fragment without an intrinsic positive charge produces
    Figure Legend Snippet: (a) CID on the LTQ-Orbitrap of the crosslinked homodimer synthetic peptide (Ac-QIGKGVAR) results in the same two major cleavage events observed in MALDI at the intrinsic positive charges. (b) MS 3 of the fragment without an intrinsic positive charge produces

    Techniques Used: Mass Spectrometry

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: 14-3-3 binding to LRRK2 is disrupted by multiple Parkinson's disease-associated mutations and regulates cytoplasmic localization
    Article Snippet: .. The HPLC system was coupled to a linear ion-trap–orbitrap hybrid mass spectrometer (LTQ-Orbitrap XL, Thermo Fisher Scientific) via a nanoelectrospray ion source (Proxeon Biosystems) fitted with a 5 cm Picotip FS360-20-10 emitter. ..

    Article Title: Quantitative Proteomic Discovery of Dynamic Epigenome Changes that Control Human Cytomegalovirus (HCMV) Infection *
    Article Snippet: .. The HPLC effluent was directly coupled to an LTQ-Orbitrap XL hybrid mass spectrometer (ThermoFisher Scientific, San Jose, CA) with a resolution of 30,000 for full MS followed by seven data-dependent and targeted MS/MS acquisitions. .. To quantify RNA levels of DOT1L, total cellular RNA was isolated using Tri-Reagent (Sigma). cDNA was generated using 1 μg of RNA with TaqMan Reverse Transcription Reagents (Roche), and equal volumes of cDNA was utilized for quantitative PCR (qPCR).

    Quantitation Assay:

    Article Title: Investigation of Age-Specific Behavioral and Proteomic Changes in an Animal Model of Chronic Ethanol Exposure
    Article Snippet: .. Mass spectrometric analysis for iTRAQ-based quantitation is carried out on a hybrid linear ion trap-Orbitrap mass spectrometer (LTQ Orbitrap XL, Thermo Fisher Scientific) ( see Note ). .. Several data analysis software packages can be used for processing of mass spectrometric data for relative protein quantitation by either spectral counting or iTRAQ.

    Mass Spectrometry:

    Article Title: O-Glycosylation Modulates Proprotein Convertase Activation of Angiopoietin-like Protein 3
    Article Snippet: .. The product of ANGPTL3 dodecapeptide with GalNAc-T2 was characterized by electrospray ionization-linear ion trap-Fourier transform mass spectrometry in an LTQ-Orbitrap XL hybrid spectrometer (Thermo-Scientific, Bremen, Germany), equipped with electron transfer dissociation (ETD) for peptide sequence analysis by MS/MS (MS2 ) with retention of glycan site-specific fragments. .. The sample was dissolved in methanol/water (1:1) containing 1% formic acid and introduced by direct infusion via a TriVersa NanoMate ESI-Chip interface (Advion BioSystems, Ithaca, NY), at a flow rate of ∼100 nl/min and 1.4 kV spray voltage.

    Article Title: Investigation of Age-Specific Behavioral and Proteomic Changes in an Animal Model of Chronic Ethanol Exposure
    Article Snippet: .. Mass spectrometric analysis for iTRAQ-based quantitation is carried out on a hybrid linear ion trap-Orbitrap mass spectrometer (LTQ Orbitrap XL, Thermo Fisher Scientific) ( see Note ). .. Several data analysis software packages can be used for processing of mass spectrometric data for relative protein quantitation by either spectral counting or iTRAQ.

    Article Title: Lectin Domains of Polypeptide GalNAc Transferases Exhibit Glycopeptide Binding Specificity *
    Article Snippet: .. Characterization of O-Glycosylation Sites Products of O -glycosylation reactions were characterized by electrospray ionization-linear ion trap-Fourier transform mass spectrometry (ESI-LIT-FT-MS) in an LTQ-Orbitrap XL hybrid spectrometer (Thermo-Scientific) equipped for both high energy collision-induced dissociation ( ) in an external collision cell , and electron transfer dissociation (ETD) ( ) for peptide sequence analysis by MS/MS (MS2 ) with retention of glycan site-specific fragments. .. Samples were dissolved in methanol/water (1:1) containing 1% formic acid and introduced by direct infusion via a TriVersa NanoMate ESI-Chip interface (Advion BioSystems) at a flow rate of ∼100 nl/min and 1.4 kV spray voltage.

    Article Title: 14-3-3 binding to LRRK2 is disrupted by multiple Parkinson's disease-associated mutations and regulates cytoplasmic localization
    Article Snippet: .. The HPLC system was coupled to a linear ion-trap–orbitrap hybrid mass spectrometer (LTQ-Orbitrap XL, Thermo Fisher Scientific) via a nanoelectrospray ion source (Proxeon Biosystems) fitted with a 5 cm Picotip FS360-20-10 emitter. ..

    Article Title: An Internal Standard-Assisted Synthesis and Degradation Proteomic Approach Reveals the Potential Linkage between VPS4B Depletion and Activation of Fatty Acid β-Oxidation in Breast Cancer Cells
    Article Snippet: .. LC-MS/MS Analysis Peptides were dissolved in 0.5% acetic acid (solvent A) and separated on a 10 cm reverse-phase PicoFrit spray tip (New Objective, Woburn, MA) packed in house with sub-2 μ m C18 resin (Prospereon Life Science, IL), using a nanoflow Xtreme simple liquid chromatography system (Microtech/CVC) coupled to a hybrid linear ion trap-Orbitrap mass spectrometer (LTQ Orbitrap, Thermo Scientific). .. Peptides were loaded onto the column with solvent A at a flow rate of 0.6 μ L/min and eluted with a 180 min linear gradient at a flow rate of 0.2 μ L/min.

    Article Title: Quantitative HPLC-MS analysis of nucleotide sugars in plant cells following off-line SPE sample preparation
    Article Snippet: .. Fragmentation experiments were performed on a linear ion trap–Orbitrap mass spectrometer (Model LTQ Orbitrap XL, Thermo Fisher Scientific). .. Establishing stable retention times for UDP-sugars on PGC Figure illustrates the effect of the electrical potential applied to the ESI source on chromatographic retention of UDP-sugars on PGC.

    Article Title: Quantitative Proteomic Discovery of Dynamic Epigenome Changes that Control Human Cytomegalovirus (HCMV) Infection *
    Article Snippet: .. The HPLC effluent was directly coupled to an LTQ-Orbitrap XL hybrid mass spectrometer (ThermoFisher Scientific, San Jose, CA) with a resolution of 30,000 for full MS followed by seven data-dependent and targeted MS/MS acquisitions. .. To quantify RNA levels of DOT1L, total cellular RNA was isolated using Tri-Reagent (Sigma). cDNA was generated using 1 μg of RNA with TaqMan Reverse Transcription Reagents (Roche), and equal volumes of cDNA was utilized for quantitative PCR (qPCR).

    Tandem Mass Spectroscopy:

    Article Title: O-Glycosylation Modulates Proprotein Convertase Activation of Angiopoietin-like Protein 3
    Article Snippet: .. The product of ANGPTL3 dodecapeptide with GalNAc-T2 was characterized by electrospray ionization-linear ion trap-Fourier transform mass spectrometry in an LTQ-Orbitrap XL hybrid spectrometer (Thermo-Scientific, Bremen, Germany), equipped with electron transfer dissociation (ETD) for peptide sequence analysis by MS/MS (MS2 ) with retention of glycan site-specific fragments. .. The sample was dissolved in methanol/water (1:1) containing 1% formic acid and introduced by direct infusion via a TriVersa NanoMate ESI-Chip interface (Advion BioSystems, Ithaca, NY), at a flow rate of ∼100 nl/min and 1.4 kV spray voltage.

    Article Title: Lectin Domains of Polypeptide GalNAc Transferases Exhibit Glycopeptide Binding Specificity *
    Article Snippet: .. Characterization of O-Glycosylation Sites Products of O -glycosylation reactions were characterized by electrospray ionization-linear ion trap-Fourier transform mass spectrometry (ESI-LIT-FT-MS) in an LTQ-Orbitrap XL hybrid spectrometer (Thermo-Scientific) equipped for both high energy collision-induced dissociation ( ) in an external collision cell , and electron transfer dissociation (ETD) ( ) for peptide sequence analysis by MS/MS (MS2 ) with retention of glycan site-specific fragments. .. Samples were dissolved in methanol/water (1:1) containing 1% formic acid and introduced by direct infusion via a TriVersa NanoMate ESI-Chip interface (Advion BioSystems) at a flow rate of ∼100 nl/min and 1.4 kV spray voltage.

    Article Title: Quantitative Proteomic Discovery of Dynamic Epigenome Changes that Control Human Cytomegalovirus (HCMV) Infection *
    Article Snippet: .. The HPLC effluent was directly coupled to an LTQ-Orbitrap XL hybrid mass spectrometer (ThermoFisher Scientific, San Jose, CA) with a resolution of 30,000 for full MS followed by seven data-dependent and targeted MS/MS acquisitions. .. To quantify RNA levels of DOT1L, total cellular RNA was isolated using Tri-Reagent (Sigma). cDNA was generated using 1 μg of RNA with TaqMan Reverse Transcription Reagents (Roche), and equal volumes of cDNA was utilized for quantitative PCR (qPCR).

    Sequencing:

    Article Title: O-Glycosylation Modulates Proprotein Convertase Activation of Angiopoietin-like Protein 3
    Article Snippet: .. The product of ANGPTL3 dodecapeptide with GalNAc-T2 was characterized by electrospray ionization-linear ion trap-Fourier transform mass spectrometry in an LTQ-Orbitrap XL hybrid spectrometer (Thermo-Scientific, Bremen, Germany), equipped with electron transfer dissociation (ETD) for peptide sequence analysis by MS/MS (MS2 ) with retention of glycan site-specific fragments. .. The sample was dissolved in methanol/water (1:1) containing 1% formic acid and introduced by direct infusion via a TriVersa NanoMate ESI-Chip interface (Advion BioSystems, Ithaca, NY), at a flow rate of ∼100 nl/min and 1.4 kV spray voltage.

    Article Title: Lectin Domains of Polypeptide GalNAc Transferases Exhibit Glycopeptide Binding Specificity *
    Article Snippet: .. Characterization of O-Glycosylation Sites Products of O -glycosylation reactions were characterized by electrospray ionization-linear ion trap-Fourier transform mass spectrometry (ESI-LIT-FT-MS) in an LTQ-Orbitrap XL hybrid spectrometer (Thermo-Scientific) equipped for both high energy collision-induced dissociation ( ) in an external collision cell , and electron transfer dissociation (ETD) ( ) for peptide sequence analysis by MS/MS (MS2 ) with retention of glycan site-specific fragments. .. Samples were dissolved in methanol/water (1:1) containing 1% formic acid and introduced by direct infusion via a TriVersa NanoMate ESI-Chip interface (Advion BioSystems) at a flow rate of ∼100 nl/min and 1.4 kV spray voltage.

    Liquid Chromatography:

    Article Title: An Internal Standard-Assisted Synthesis and Degradation Proteomic Approach Reveals the Potential Linkage between VPS4B Depletion and Activation of Fatty Acid β-Oxidation in Breast Cancer Cells
    Article Snippet: .. LC-MS/MS Analysis Peptides were dissolved in 0.5% acetic acid (solvent A) and separated on a 10 cm reverse-phase PicoFrit spray tip (New Objective, Woburn, MA) packed in house with sub-2 μ m C18 resin (Prospereon Life Science, IL), using a nanoflow Xtreme simple liquid chromatography system (Microtech/CVC) coupled to a hybrid linear ion trap-Orbitrap mass spectrometer (LTQ Orbitrap, Thermo Scientific). .. Peptides were loaded onto the column with solvent A at a flow rate of 0.6 μ L/min and eluted with a 180 min linear gradient at a flow rate of 0.2 μ L/min.

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  • 99
    Thermo Fisher hybrid linear quadrupole ion trap orbitrap mass spectrometer
    (a) CID on the <t>LTQ-Orbitrap</t> of the crosslinked homodimer synthetic peptide (Ac-QIGKGVAR) results in the same two major cleavage events observed in MALDI at the intrinsic positive charges. (b) MS 3 of the fragment without an intrinsic positive charge produces
    Hybrid Linear Quadrupole Ion Trap Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid linear quadrupole ion trap orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    hybrid linear quadrupole ion trap orbitrap mass spectrometer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher hybrid dual cell quadrupole linear ion trap orbitrap mass spectrometer
    Multi-dissociation cell installed onto a hybrid <t>QLT-Orbitrap</t> mass spectrometer. In place of the pre-existing HCD cell (A), we installed a custom ion-ion reaction vessel (multi-dissociation cell, B–D). The multi-dissociation cell (MDC) comprises
    Hybrid Dual Cell Quadrupole Linear Ion Trap Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid dual cell quadrupole linear ion trap orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hybrid dual cell quadrupole linear ion trap orbitrap mass spectrometer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher hybrid linear quadrupole ion trap mass spectrometer
    Multi-dissociation cell installed onto a hybrid <t>QLT-Orbitrap</t> mass spectrometer. In place of the pre-existing HCD cell (A), we installed a custom ion-ion reaction vessel (multi-dissociation cell, B–D). The multi-dissociation cell (MDC) comprises
    Hybrid Linear Quadrupole Ion Trap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid linear quadrupole ion trap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hybrid linear quadrupole ion trap mass spectrometer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    (a) CID on the LTQ-Orbitrap of the crosslinked homodimer synthetic peptide (Ac-QIGKGVAR) results in the same two major cleavage events observed in MALDI at the intrinsic positive charges. (b) MS 3 of the fragment without an intrinsic positive charge produces

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Quaternary Diamines as Mass Spectrometry Cleavable Crosslinkers for Protein Interactions

    doi: 10.1007/s13361-011-0288-4

    Figure Lengend Snippet: (a) CID on the LTQ-Orbitrap of the crosslinked homodimer synthetic peptide (Ac-QIGKGVAR) results in the same two major cleavage events observed in MALDI at the intrinsic positive charges. (b) MS 3 of the fragment without an intrinsic positive charge produces

    Article Snippet: The crosslinked standard peptides were analyzed with a hybrid linear quadrupole ion trap-Orbitrap mass spectrometer (ThermoFisher Scientific, Inc. model LTQ-Orbitrap XL; San Jose, CA, USA).

    Techniques: Mass Spectrometry

    Multi-dissociation cell installed onto a hybrid QLT-Orbitrap mass spectrometer. In place of the pre-existing HCD cell (A), we installed a custom ion-ion reaction vessel (multi-dissociation cell, B–D). The multi-dissociation cell (MDC) comprises

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Multipurpose Dissociation Cell for Enhanced ETD of Intact Protein Species

    doi: 10.1007/s13361-013-0622-0

    Figure Lengend Snippet: Multi-dissociation cell installed onto a hybrid QLT-Orbitrap mass spectrometer. In place of the pre-existing HCD cell (A), we installed a custom ion-ion reaction vessel (multi-dissociation cell, B–D). The multi-dissociation cell (MDC) comprises

    Article Snippet: Here, we describe a modified hybrid dual cell quadrupole linear ion trap-Orbitrap mass spectrometer, LTQ Orbitrap Velos ETD, (Thermo Fisher Scientific, Bremen Germany).

    Techniques: Mass Spectrometry

    Solid phase extraction-based strong cation exchange (A) Shown is the solution charge state distribution of 38,487 phosphorylated and unphosphorylated peptides from lysC digested yeast whole-cell extract subjected to the described SPE/SCX/IMAC enrichment protocol analyzed by LC-MS/MS on a LTQ Orbitrap Velos. (B) The peptide overlap between SCX fractions is shown as the fraction of all peptides that were identified in 1, 2, 3, or more different fractions. (C) A comparison of the solution charge states of the identified phosphopeptides from trypsin and lysC digested yeast whole cell extracts.

    Journal: Methods (San Diego, Calif.)

    Article Title: A solid phase extraction-based platform for rapid phosphoproteomic analysis

    doi: 10.1016/j.ymeth.2011.03.008

    Figure Lengend Snippet: Solid phase extraction-based strong cation exchange (A) Shown is the solution charge state distribution of 38,487 phosphorylated and unphosphorylated peptides from lysC digested yeast whole-cell extract subjected to the described SPE/SCX/IMAC enrichment protocol analyzed by LC-MS/MS on a LTQ Orbitrap Velos. (B) The peptide overlap between SCX fractions is shown as the fraction of all peptides that were identified in 1, 2, 3, or more different fractions. (C) A comparison of the solution charge states of the identified phosphopeptides from trypsin and lysC digested yeast whole cell extracts.

    Article Snippet: Peptides were detected in a hybrid dual-cell quadrupole linear ion trap – orbitrap mass spectrometer (LTQ Orbitrap Velos, ThermoFisher) using a data-dependent Top20 method, or the single-cell linear ion trap – orbitrap mass spectrometer (LTQ Orbitrap Discovery, ThermoFisher) using a Top10 method.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry