immunosorbent assay elisa kit  (Echelon Biosciences)


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    Echelon Biosciences immunosorbent assay elisa kit
    Immunosorbent Assay Elisa Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    immunosorbent assay elisa kit  (Echelon Biosciences)


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    Echelon Biosciences immunosorbent assay elisa kit
    Immunosorbent Assay Elisa Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hyaluronan hyaluronic acid enzyme  (Echelon Biosciences)


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    Echelon Biosciences hyaluronan hyaluronic acid enzyme
    (A) <t>Hyaluronan</t> in media of confluent orbital fibroblast cultures from GO (n = 3) and normal (n = 3) individuals pretreated with 0, 10, 50 or 100 µM quercetin for 24 h before IL-1β stimulation (10 ng/ml, 16 h). (B) The effect of quercetin on hyaluronan production in GO orbital fibroblasts (n = 3) stimulated with IL-1β (10 ng/ml, 16 h) or TNF-α (10 ng/ml, 16 h). Triplicate measurements were averaged, and the data are expressed as mean values ± SD. * P <0.05 vs. cells stimulated with IL-1β or TNF-α alone.
    Hyaluronan Hyaluronic Acid Enzyme, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Quercetin Inhibits IL-1β-Induced Inflammation, Hyaluronan Production and Adipogenesis in Orbital Fibroblasts from Graves' Orbitopathy"

    Article Title: Quercetin Inhibits IL-1β-Induced Inflammation, Hyaluronan Production and Adipogenesis in Orbital Fibroblasts from Graves' Orbitopathy

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0026261

    (A) Hyaluronan in media of confluent orbital fibroblast cultures from GO (n = 3) and normal (n = 3) individuals pretreated with 0, 10, 50 or 100 µM quercetin for 24 h before IL-1β stimulation (10 ng/ml, 16 h). (B) The effect of quercetin on hyaluronan production in GO orbital fibroblasts (n = 3) stimulated with IL-1β (10 ng/ml, 16 h) or TNF-α (10 ng/ml, 16 h). Triplicate measurements were averaged, and the data are expressed as mean values ± SD. * P <0.05 vs. cells stimulated with IL-1β or TNF-α alone.
    Figure Legend Snippet: (A) Hyaluronan in media of confluent orbital fibroblast cultures from GO (n = 3) and normal (n = 3) individuals pretreated with 0, 10, 50 or 100 µM quercetin for 24 h before IL-1β stimulation (10 ng/ml, 16 h). (B) The effect of quercetin on hyaluronan production in GO orbital fibroblasts (n = 3) stimulated with IL-1β (10 ng/ml, 16 h) or TNF-α (10 ng/ml, 16 h). Triplicate measurements were averaged, and the data are expressed as mean values ± SD. * P <0.05 vs. cells stimulated with IL-1β or TNF-α alone.

    Techniques Used:

    elisa kit  (Echelon Biosciences)


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    Echelon Biosciences elisa kit
    Elisa Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    elisa kit - by Bioz Stars, 2023-02
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    immunosorbent assay elisa  (Echelon Biosciences)


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    Echelon Biosciences immunosorbent assay elisa
    Immunosorbent Assay Elisa, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunosorbent assay elisa/product/Echelon Biosciences
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    immunosorbent assay elisa - by Bioz Stars, 2023-02
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    hyaluronan concentrations  (Echelon Biosciences)


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    Echelon Biosciences hyaluronan concentrations
    Serum levels of <t>hyaluronan</t> within 48 h after out-of-hospital cardiac arrest. Significant increase of hyaluronan levels within 48 h after resuscitation; bars represent mean values with error bars representing ±1 SE. MANOVA analysis for repeated measurements; p = 0.043.
    Hyaluronan Concentrations, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "On-the-Scene Hyaluronan and Syndecan-1 Serum Concentrations and Outcome after Cardiac Arrest and Resuscitation"

    Article Title: On-the-Scene Hyaluronan and Syndecan-1 Serum Concentrations and Outcome after Cardiac Arrest and Resuscitation

    Journal: Mediators of Inflammation

    doi: 10.1155/2019/8071619

    Serum levels of hyaluronan within 48 h after out-of-hospital cardiac arrest. Significant increase of hyaluronan levels within 48 h after resuscitation; bars represent mean values with error bars representing ±1 SE. MANOVA analysis for repeated measurements; p = 0.043.
    Figure Legend Snippet: Serum levels of hyaluronan within 48 h after out-of-hospital cardiac arrest. Significant increase of hyaluronan levels within 48 h after resuscitation; bars represent mean values with error bars representing ±1 SE. MANOVA analysis for repeated measurements; p = 0.043.

    Techniques Used:

    Plots of correlation between hyaluronan levels and 30-day survival 0 h-48 h after cardiac arrest. Significant correlation of hyaluronan levels and survival 0 h-24 h after ROSC; bars represent mean values with error bars representing ±1 SE. MANOVA analysis of variances and the post hoc ∗ Mann-Whitney U test; p ≤ 0.05; survivors n = 9, nonsurvivors n = 6.
    Figure Legend Snippet: Plots of correlation between hyaluronan levels and 30-day survival 0 h-48 h after cardiac arrest. Significant correlation of hyaluronan levels and survival 0 h-24 h after ROSC; bars represent mean values with error bars representing ±1 SE. MANOVA analysis of variances and the post hoc ∗ Mann-Whitney U test; p ≤ 0.05; survivors n = 9, nonsurvivors n = 6.

    Techniques Used: MANN-WHITNEY

    Plots of correlation between hyaluronan concentrations and MOF score 48 h after cardiac arrest. Significant correlation between hyaluronan release, time, and multiple organ failure 0 h-48 h after ROSC; bars represent mean values with error bars representing ±1 SE. ANOVA analysis of variances and the post hoc Mann-Whitney rank sum test; p ≤ 0.05; MOF ≤ 6: n = 9, MOF > 6: n = 4.
    Figure Legend Snippet: Plots of correlation between hyaluronan concentrations and MOF score 48 h after cardiac arrest. Significant correlation between hyaluronan release, time, and multiple organ failure 0 h-48 h after ROSC; bars represent mean values with error bars representing ±1 SE. ANOVA analysis of variances and the post hoc Mann-Whitney rank sum test; p ≤ 0.05; MOF ≤ 6: n = 9, MOF > 6: n = 4.

    Techniques Used: MANN-WHITNEY

    immunosorbent assay  (Echelon Biosciences)


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    Echelon Biosciences immunosorbent assay
    Immunosorbent Assay, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    immunosorbent assay - by Bioz Stars, 2023-02
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    hyaluronan  (Echelon Biosciences)


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    Echelon Biosciences hyaluronan
    Hyaluronan, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hyaluronan - by Bioz Stars, 2023-02
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    hyaluronic acid  (Echelon Biosciences)


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    Echelon Biosciences hyaluronic acid
    Hyaluronic Acid, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hyaluronan content  (Echelon Biosciences)


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    Echelon Biosciences hyaluronan content
    A) Percent difference <t>hyaluronan</t> concentration (ng/mL) when compared to 0% strain. B) Integrated density of hyaluronan staining (green, Alexa Fluor-488) normalized to fluorescent intensity of actin staining (red, Phalloidin-565). C) Representative immunofluorescent images of DAPI, hyaluronan, and F-actin stains and a composite image of all strain groups. Dots represent individual samples and significant differences between groups are shown with brackets. Scale bar = 50 µm.
    Hyaluronan Content, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Endogenous production of hyaluronan, PRG4, and cytokines is sensitive to cyclic loading in synoviocytes"

    Article Title: Endogenous production of hyaluronan, PRG4, and cytokines is sensitive to cyclic loading in synoviocytes

    Journal: bioRxiv

    doi: 10.1101/2022.04.20.488903

    A) Percent difference hyaluronan concentration (ng/mL) when compared to 0% strain. B) Integrated density of hyaluronan staining (green, Alexa Fluor-488) normalized to fluorescent intensity of actin staining (red, Phalloidin-565). C) Representative immunofluorescent images of DAPI, hyaluronan, and F-actin stains and a composite image of all strain groups. Dots represent individual samples and significant differences between groups are shown with brackets. Scale bar = 50 µm.
    Figure Legend Snippet: A) Percent difference hyaluronan concentration (ng/mL) when compared to 0% strain. B) Integrated density of hyaluronan staining (green, Alexa Fluor-488) normalized to fluorescent intensity of actin staining (red, Phalloidin-565). C) Representative immunofluorescent images of DAPI, hyaluronan, and F-actin stains and a composite image of all strain groups. Dots represent individual samples and significant differences between groups are shown with brackets. Scale bar = 50 µm.

    Techniques Used: Concentration Assay, Staining

    A) sPLS-DA to predict strain group from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. B) sPLSR to predict hyaluronan concentration group from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. C) sPLSR to predict PRG4 concentration from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. sPLS plots are shown in the X representative space of data along 2 components.
    Figure Legend Snippet: A) sPLS-DA to predict strain group from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. B) sPLSR to predict hyaluronan concentration group from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. C) sPLSR to predict PRG4 concentration from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. sPLS plots are shown in the X representative space of data along 2 components.

    Techniques Used: Expressing, Concentration Assay

    Q 2 total values of each component for A) gene expression predicting hyaluronan concentration, B) gene expression predicting PRG4 concentration, C) cytokine concentration predicting hyaluronan concentration, and D) cytokine concentration prediction PRG4 concentration.
    Figure Legend Snippet: Q 2 total values of each component for A) gene expression predicting hyaluronan concentration, B) gene expression predicting PRG4 concentration, C) cytokine concentration predicting hyaluronan concentration, and D) cytokine concentration prediction PRG4 concentration.

    Techniques Used: Expressing, Concentration Assay

    A) sPLS-DA to predict strain group from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. B) sPLSR to predict hyaluronan concentration group from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. C) sPLSR to predict PRG4 concentration from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. sPLS plots are shown in the X representative space of data along 2 components.
    Figure Legend Snippet: A) sPLS-DA to predict strain group from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. B) sPLSR to predict hyaluronan concentration group from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. C) sPLSR to predict PRG4 concentration from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. sPLS plots are shown in the X representative space of data along 2 components.

    Techniques Used: Concentration Assay

    peritoneal fluid  (Echelon Biosciences)


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    Echelon Biosciences peritoneal fluid
    Peritoneal Fluid, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Echelon Biosciences immunosorbent assay elisa kit
    Immunosorbent Assay Elisa Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) <t>Hyaluronan</t> in media of confluent orbital fibroblast cultures from GO (n = 3) and normal (n = 3) individuals pretreated with 0, 10, 50 or 100 µM quercetin for 24 h before IL-1β stimulation (10 ng/ml, 16 h). (B) The effect of quercetin on hyaluronan production in GO orbital fibroblasts (n = 3) stimulated with IL-1β (10 ng/ml, 16 h) or TNF-α (10 ng/ml, 16 h). Triplicate measurements were averaged, and the data are expressed as mean values ± SD. * P <0.05 vs. cells stimulated with IL-1β or TNF-α alone.
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    (A) <t>Hyaluronan</t> in media of confluent orbital fibroblast cultures from GO (n = 3) and normal (n = 3) individuals pretreated with 0, 10, 50 or 100 µM quercetin for 24 h before IL-1β stimulation (10 ng/ml, 16 h). (B) The effect of quercetin on hyaluronan production in GO orbital fibroblasts (n = 3) stimulated with IL-1β (10 ng/ml, 16 h) or TNF-α (10 ng/ml, 16 h). Triplicate measurements were averaged, and the data are expressed as mean values ± SD. * P <0.05 vs. cells stimulated with IL-1β or TNF-α alone.
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    (A) <t>Hyaluronan</t> in media of confluent orbital fibroblast cultures from GO (n = 3) and normal (n = 3) individuals pretreated with 0, 10, 50 or 100 µM quercetin for 24 h before IL-1β stimulation (10 ng/ml, 16 h). (B) The effect of quercetin on hyaluronan production in GO orbital fibroblasts (n = 3) stimulated with IL-1β (10 ng/ml, 16 h) or TNF-α (10 ng/ml, 16 h). Triplicate measurements were averaged, and the data are expressed as mean values ± SD. * P <0.05 vs. cells stimulated with IL-1β or TNF-α alone.
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    Serum levels of <t>hyaluronan</t> within 48 h after out-of-hospital cardiac arrest. Significant increase of hyaluronan levels within 48 h after resuscitation; bars represent mean values with error bars representing ±1 SE. MANOVA analysis for repeated measurements; p = 0.043.
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    Serum levels of <t>hyaluronan</t> within 48 h after out-of-hospital cardiac arrest. Significant increase of hyaluronan levels within 48 h after resuscitation; bars represent mean values with error bars representing ±1 SE. MANOVA analysis for repeated measurements; p = 0.043.
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    Echelon Biosciences hyaluronan
    Serum levels of <t>hyaluronan</t> within 48 h after out-of-hospital cardiac arrest. Significant increase of hyaluronan levels within 48 h after resuscitation; bars represent mean values with error bars representing ±1 SE. MANOVA analysis for repeated measurements; p = 0.043.
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    Serum levels of <t>hyaluronan</t> within 48 h after out-of-hospital cardiac arrest. Significant increase of hyaluronan levels within 48 h after resuscitation; bars represent mean values with error bars representing ±1 SE. MANOVA analysis for repeated measurements; p = 0.043.
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    Echelon Biosciences hyaluronan content
    A) Percent difference <t>hyaluronan</t> concentration (ng/mL) when compared to 0% strain. B) Integrated density of hyaluronan staining (green, Alexa Fluor-488) normalized to fluorescent intensity of actin staining (red, Phalloidin-565). C) Representative immunofluorescent images of DAPI, hyaluronan, and F-actin stains and a composite image of all strain groups. Dots represent individual samples and significant differences between groups are shown with brackets. Scale bar = 50 µm.
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    A) Percent difference <t>hyaluronan</t> concentration (ng/mL) when compared to 0% strain. B) Integrated density of hyaluronan staining (green, Alexa Fluor-488) normalized to fluorescent intensity of actin staining (red, Phalloidin-565). C) Representative immunofluorescent images of DAPI, hyaluronan, and F-actin stains and a composite image of all strain groups. Dots represent individual samples and significant differences between groups are shown with brackets. Scale bar = 50 µm.
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    (A) Hyaluronan in media of confluent orbital fibroblast cultures from GO (n = 3) and normal (n = 3) individuals pretreated with 0, 10, 50 or 100 µM quercetin for 24 h before IL-1β stimulation (10 ng/ml, 16 h). (B) The effect of quercetin on hyaluronan production in GO orbital fibroblasts (n = 3) stimulated with IL-1β (10 ng/ml, 16 h) or TNF-α (10 ng/ml, 16 h). Triplicate measurements were averaged, and the data are expressed as mean values ± SD. * P <0.05 vs. cells stimulated with IL-1β or TNF-α alone.

    Journal: PLoS ONE

    Article Title: Quercetin Inhibits IL-1β-Induced Inflammation, Hyaluronan Production and Adipogenesis in Orbital Fibroblasts from Graves' Orbitopathy

    doi: 10.1371/journal.pone.0026261

    Figure Lengend Snippet: (A) Hyaluronan in media of confluent orbital fibroblast cultures from GO (n = 3) and normal (n = 3) individuals pretreated with 0, 10, 50 or 100 µM quercetin for 24 h before IL-1β stimulation (10 ng/ml, 16 h). (B) The effect of quercetin on hyaluronan production in GO orbital fibroblasts (n = 3) stimulated with IL-1β (10 ng/ml, 16 h) or TNF-α (10 ng/ml, 16 h). Triplicate measurements were averaged, and the data are expressed as mean values ± SD. * P <0.05 vs. cells stimulated with IL-1β or TNF-α alone.

    Article Snippet: Annexin V-FITC apoptosis detection kit was purchased from BD Biosciences (Franklin Lakes, NJ).A hyaluronan (hyaluronic acid) enzyme-linked immunosorbent assay (ELISA) kit was purchased from Echelon Biosciences (Salt Lake, UT, USA).

    Techniques:

    Serum levels of hyaluronan within 48 h after out-of-hospital cardiac arrest. Significant increase of hyaluronan levels within 48 h after resuscitation; bars represent mean values with error bars representing ±1 SE. MANOVA analysis for repeated measurements; p = 0.043.

    Journal: Mediators of Inflammation

    Article Title: On-the-Scene Hyaluronan and Syndecan-1 Serum Concentrations and Outcome after Cardiac Arrest and Resuscitation

    doi: 10.1155/2019/8071619

    Figure Lengend Snippet: Serum levels of hyaluronan within 48 h after out-of-hospital cardiac arrest. Significant increase of hyaluronan levels within 48 h after resuscitation; bars represent mean values with error bars representing ±1 SE. MANOVA analysis for repeated measurements; p = 0.043.

    Article Snippet: Hyaluronan concentrations were quantified by enzyme-linked immunosorbent assay (Hyaluronan Enzyme-Linked Immunosorbent Assay Kit (HA-ELISA) K-1200; Echelon Biosciences Inc., Salt Lake City, UT, USA).

    Techniques:

    Plots of correlation between hyaluronan levels and 30-day survival 0 h-48 h after cardiac arrest. Significant correlation of hyaluronan levels and survival 0 h-24 h after ROSC; bars represent mean values with error bars representing ±1 SE. MANOVA analysis of variances and the post hoc ∗ Mann-Whitney U test; p ≤ 0.05; survivors n = 9, nonsurvivors n = 6.

    Journal: Mediators of Inflammation

    Article Title: On-the-Scene Hyaluronan and Syndecan-1 Serum Concentrations and Outcome after Cardiac Arrest and Resuscitation

    doi: 10.1155/2019/8071619

    Figure Lengend Snippet: Plots of correlation between hyaluronan levels and 30-day survival 0 h-48 h after cardiac arrest. Significant correlation of hyaluronan levels and survival 0 h-24 h after ROSC; bars represent mean values with error bars representing ±1 SE. MANOVA analysis of variances and the post hoc ∗ Mann-Whitney U test; p ≤ 0.05; survivors n = 9, nonsurvivors n = 6.

    Article Snippet: Hyaluronan concentrations were quantified by enzyme-linked immunosorbent assay (Hyaluronan Enzyme-Linked Immunosorbent Assay Kit (HA-ELISA) K-1200; Echelon Biosciences Inc., Salt Lake City, UT, USA).

    Techniques: MANN-WHITNEY

    Plots of correlation between hyaluronan concentrations and MOF score 48 h after cardiac arrest. Significant correlation between hyaluronan release, time, and multiple organ failure 0 h-48 h after ROSC; bars represent mean values with error bars representing ±1 SE. ANOVA analysis of variances and the post hoc Mann-Whitney rank sum test; p ≤ 0.05; MOF ≤ 6: n = 9, MOF > 6: n = 4.

    Journal: Mediators of Inflammation

    Article Title: On-the-Scene Hyaluronan and Syndecan-1 Serum Concentrations and Outcome after Cardiac Arrest and Resuscitation

    doi: 10.1155/2019/8071619

    Figure Lengend Snippet: Plots of correlation between hyaluronan concentrations and MOF score 48 h after cardiac arrest. Significant correlation between hyaluronan release, time, and multiple organ failure 0 h-48 h after ROSC; bars represent mean values with error bars representing ±1 SE. ANOVA analysis of variances and the post hoc Mann-Whitney rank sum test; p ≤ 0.05; MOF ≤ 6: n = 9, MOF > 6: n = 4.

    Article Snippet: Hyaluronan concentrations were quantified by enzyme-linked immunosorbent assay (Hyaluronan Enzyme-Linked Immunosorbent Assay Kit (HA-ELISA) K-1200; Echelon Biosciences Inc., Salt Lake City, UT, USA).

    Techniques: MANN-WHITNEY

    A) Percent difference hyaluronan concentration (ng/mL) when compared to 0% strain. B) Integrated density of hyaluronan staining (green, Alexa Fluor-488) normalized to fluorescent intensity of actin staining (red, Phalloidin-565). C) Representative immunofluorescent images of DAPI, hyaluronan, and F-actin stains and a composite image of all strain groups. Dots represent individual samples and significant differences between groups are shown with brackets. Scale bar = 50 µm.

    Journal: bioRxiv

    Article Title: Endogenous production of hyaluronan, PRG4, and cytokines is sensitive to cyclic loading in synoviocytes

    doi: 10.1101/2022.04.20.488903

    Figure Lengend Snippet: A) Percent difference hyaluronan concentration (ng/mL) when compared to 0% strain. B) Integrated density of hyaluronan staining (green, Alexa Fluor-488) normalized to fluorescent intensity of actin staining (red, Phalloidin-565). C) Representative immunofluorescent images of DAPI, hyaluronan, and F-actin stains and a composite image of all strain groups. Dots represent individual samples and significant differences between groups are shown with brackets. Scale bar = 50 µm.

    Article Snippet: Samples were then dialyzed against deionized water overnight at room temperature before measuring hyaluronan content in triplicate with a commercially available competitive ELISA-like assay (#K-1200, Echelon Biosciences, Salt Lake City, UT) read using a multiplate reader (Biotek Synergy H4).

    Techniques: Concentration Assay, Staining

    A) sPLS-DA to predict strain group from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. B) sPLSR to predict hyaluronan concentration group from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. C) sPLSR to predict PRG4 concentration from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. sPLS plots are shown in the X representative space of data along 2 components.

    Journal: bioRxiv

    Article Title: Endogenous production of hyaluronan, PRG4, and cytokines is sensitive to cyclic loading in synoviocytes

    doi: 10.1101/2022.04.20.488903

    Figure Lengend Snippet: A) sPLS-DA to predict strain group from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. B) sPLSR to predict hyaluronan concentration group from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. C) sPLSR to predict PRG4 concentration from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. sPLS plots are shown in the X representative space of data along 2 components.

    Article Snippet: Samples were then dialyzed against deionized water overnight at room temperature before measuring hyaluronan content in triplicate with a commercially available competitive ELISA-like assay (#K-1200, Echelon Biosciences, Salt Lake City, UT) read using a multiplate reader (Biotek Synergy H4).

    Techniques: Expressing, Concentration Assay

    Q 2 total values of each component for A) gene expression predicting hyaluronan concentration, B) gene expression predicting PRG4 concentration, C) cytokine concentration predicting hyaluronan concentration, and D) cytokine concentration prediction PRG4 concentration.

    Journal: bioRxiv

    Article Title: Endogenous production of hyaluronan, PRG4, and cytokines is sensitive to cyclic loading in synoviocytes

    doi: 10.1101/2022.04.20.488903

    Figure Lengend Snippet: Q 2 total values of each component for A) gene expression predicting hyaluronan concentration, B) gene expression predicting PRG4 concentration, C) cytokine concentration predicting hyaluronan concentration, and D) cytokine concentration prediction PRG4 concentration.

    Article Snippet: Samples were then dialyzed against deionized water overnight at room temperature before measuring hyaluronan content in triplicate with a commercially available competitive ELISA-like assay (#K-1200, Echelon Biosciences, Salt Lake City, UT) read using a multiplate reader (Biotek Synergy H4).

    Techniques: Expressing, Concentration Assay

    A) sPLS-DA to predict strain group from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. B) sPLSR to predict hyaluronan concentration group from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. C) sPLSR to predict PRG4 concentration from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. sPLS plots are shown in the X representative space of data along 2 components.

    Journal: bioRxiv

    Article Title: Endogenous production of hyaluronan, PRG4, and cytokines is sensitive to cyclic loading in synoviocytes

    doi: 10.1101/2022.04.20.488903

    Figure Lengend Snippet: A) sPLS-DA to predict strain group from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. B) sPLSR to predict hyaluronan concentration group from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. C) sPLSR to predict PRG4 concentration from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. sPLS plots are shown in the X representative space of data along 2 components.

    Article Snippet: Samples were then dialyzed against deionized water overnight at room temperature before measuring hyaluronan content in triplicate with a commercially available competitive ELISA-like assay (#K-1200, Echelon Biosciences, Salt Lake City, UT) read using a multiplate reader (Biotek Synergy H4).

    Techniques: Concentration Assay