Journal: PLoS ONE

Article Title: Quercetin Inhibits IL-1β-Induced Inflammation, Hyaluronan Production and Adipogenesis in Orbital Fibroblasts from Graves' Orbitopathy

doi: 10.1371/journal.pone.0026261

Figure Lengend Snippet: (A) Hyaluronan in media of confluent orbital fibroblast cultures from GO (n = 3) and normal (n = 3) individuals pretreated with 0, 10, 50 or 100 µM quercetin for 24 h before IL-1β stimulation (10 ng/ml, 16 h). (B) The effect of quercetin on hyaluronan production in GO orbital fibroblasts (n = 3) stimulated with IL-1β (10 ng/ml, 16 h) or TNF-α (10 ng/ml, 16 h). Triplicate measurements were averaged, and the data are expressed as mean values ± SD. * P <0.05 vs. cells stimulated with IL-1β or TNF-α alone.

Article Snippet: Annexin V-FITC apoptosis detection kit was purchased from BD Biosciences (Franklin Lakes, NJ).A hyaluronan (hyaluronic acid) enzyme-linked immunosorbent assay (ELISA) kit was purchased from Echelon Biosciences (Salt Lake, UT, USA).

Techniques:

Journal: Mediators of Inflammation

Article Title: On-the-Scene Hyaluronan and Syndecan-1 Serum Concentrations and Outcome after Cardiac Arrest and Resuscitation

doi: 10.1155/2019/8071619

Figure Lengend Snippet: Serum levels of hyaluronan within 48 h after out-of-hospital cardiac arrest. Significant increase of hyaluronan levels within 48 h after resuscitation; bars represent mean values with error bars representing ±1 SE. MANOVA analysis for repeated measurements; p = 0.043.

Article Snippet: Hyaluronan concentrations were quantified by enzyme-linked immunosorbent assay (Hyaluronan Enzyme-Linked Immunosorbent Assay Kit (HA-ELISA) K-1200; Echelon Biosciences Inc., Salt Lake City, UT, USA).

Techniques:

Journal: Mediators of Inflammation

Article Title: On-the-Scene Hyaluronan and Syndecan-1 Serum Concentrations and Outcome after Cardiac Arrest and Resuscitation

doi: 10.1155/2019/8071619

Figure Lengend Snippet: Plots of correlation between hyaluronan levels and 30-day survival 0 h-48 h after cardiac arrest. Significant correlation of hyaluronan levels and survival 0 h-24 h after ROSC; bars represent mean values with error bars representing ±1 SE. MANOVA analysis of variances and the post hoc ∗ Mann-Whitney U test; p ≤ 0.05; survivors n = 9, nonsurvivors n = 6.

Article Snippet: Hyaluronan concentrations were quantified by enzyme-linked immunosorbent assay (Hyaluronan Enzyme-Linked Immunosorbent Assay Kit (HA-ELISA) K-1200; Echelon Biosciences Inc., Salt Lake City, UT, USA).

Techniques: MANN-WHITNEY

Journal: Mediators of Inflammation

Article Title: On-the-Scene Hyaluronan and Syndecan-1 Serum Concentrations and Outcome after Cardiac Arrest and Resuscitation

doi: 10.1155/2019/8071619

Figure Lengend Snippet: Plots of correlation between hyaluronan concentrations and MOF score 48 h after cardiac arrest. Significant correlation between hyaluronan release, time, and multiple organ failure 0 h-48 h after ROSC; bars represent mean values with error bars representing ±1 SE. ANOVA analysis of variances and the post hoc Mann-Whitney rank sum test; p ≤ 0.05; MOF ≤ 6: n = 9, MOF > 6: n = 4.

Article Snippet: Hyaluronan concentrations were quantified by enzyme-linked immunosorbent assay (Hyaluronan Enzyme-Linked Immunosorbent Assay Kit (HA-ELISA) K-1200; Echelon Biosciences Inc., Salt Lake City, UT, USA).

Techniques: MANN-WHITNEY

Journal: bioRxiv

Article Title: Endogenous production of hyaluronan, PRG4, and cytokines is sensitive to cyclic loading in synoviocytes

doi: 10.1101/2022.04.20.488903

Figure Lengend Snippet: A) Percent difference hyaluronan concentration (ng/mL) when compared to 0% strain. B) Integrated density of hyaluronan staining (green, Alexa Fluor-488) normalized to fluorescent intensity of actin staining (red, Phalloidin-565). C) Representative immunofluorescent images of DAPI, hyaluronan, and F-actin stains and a composite image of all strain groups. Dots represent individual samples and significant differences between groups are shown with brackets. Scale bar = 50 µm.

Article Snippet: Samples were then dialyzed against deionized water overnight at room temperature before measuring hyaluronan content in triplicate with a commercially available competitive ELISA-like assay (#K-1200, Echelon Biosciences, Salt Lake City, UT) read using a multiplate reader (Biotek Synergy H4).

Techniques: Concentration Assay, Staining

Journal: bioRxiv

Article Title: Endogenous production of hyaluronan, PRG4, and cytokines is sensitive to cyclic loading in synoviocytes

doi: 10.1101/2022.04.20.488903

Figure Lengend Snippet: A) sPLS-DA to predict strain group from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. B) sPLSR to predict hyaluronan concentration group from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. C) sPLSR to predict PRG4 concentration from gene expression data, along with corresponding loadings plots, showing the contributions of each gene for each component. sPLS plots are shown in the X representative space of data along 2 components.

Article Snippet: Samples were then dialyzed against deionized water overnight at room temperature before measuring hyaluronan content in triplicate with a commercially available competitive ELISA-like assay (#K-1200, Echelon Biosciences, Salt Lake City, UT) read using a multiplate reader (Biotek Synergy H4).

Techniques: Expressing, Concentration Assay

Journal: bioRxiv

Article Title: Endogenous production of hyaluronan, PRG4, and cytokines is sensitive to cyclic loading in synoviocytes

doi: 10.1101/2022.04.20.488903

Figure Lengend Snippet: Q 2 total values of each component for A) gene expression predicting hyaluronan concentration, B) gene expression predicting PRG4 concentration, C) cytokine concentration predicting hyaluronan concentration, and D) cytokine concentration prediction PRG4 concentration.

Article Snippet: Samples were then dialyzed against deionized water overnight at room temperature before measuring hyaluronan content in triplicate with a commercially available competitive ELISA-like assay (#K-1200, Echelon Biosciences, Salt Lake City, UT) read using a multiplate reader (Biotek Synergy H4).

Techniques: Expressing, Concentration Assay

Journal: bioRxiv

Article Title: Endogenous production of hyaluronan, PRG4, and cytokines is sensitive to cyclic loading in synoviocytes

doi: 10.1101/2022.04.20.488903

Figure Lengend Snippet: A) sPLS-DA to predict strain group from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. B) sPLSR to predict hyaluronan concentration group from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. C) sPLSR to predict PRG4 concentration from cytokine concentration data, along with corresponding loadings plots, showing the contributions of each cytokine for each component. sPLS plots are shown in the X representative space of data along 2 components.

Article Snippet: Samples were then dialyzed against deionized water overnight at room temperature before measuring hyaluronan content in triplicate with a commercially available competitive ELISA-like assay (#K-1200, Echelon Biosciences, Salt Lake City, UT) read using a multiplate reader (Biotek Synergy H4).

Techniques: Concentration Assay