Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology
Article Title: Upregulation of airway smooth muscle calcium-sensing receptor by low-molecular-weight hyaluronan
doi: 10.1152/ajplung.00429.2019
Figure Lengend Snippet: A: concentrations of hyaluronan (HA) in bronchoalveolar lavage fluid (BALF) at 1 and 24 h postexposure to Br2 (600 ppm for 30 min), measured by ELISA. Individual values and means ± 1 SE; number of mice for each condition as follows: air = 8; 1 h and 24 h postexposure to Br2 = 9 each. One-way analysis of variance was performed followed by the Tukey t test. B, left: agar gel electrophoresis of Yabro (a form of high-molecular-weight hyaluronan; 3 mg/mL) before and following exposure to Br2. Lane 1: HA Mega-HA Ladder (Hyalose); lane 2: select-HA Hiladder; lane 3: Yabro; lane 4: Yabro exposed to Br2 (400 ppm for 30 min) and stored at 4°C for 1 or 24 h. B, right: agar gel electrophoresis of concentrated BALF from air and Br2-exposed mice. Lane 1: select-HA Hiladder; Lane 2: select-HA LoLadder. The different lanes show the appearance of BALF at the indicated times postexposure. For the last lane, BALF was obtained at 24 h postexposure to Br2 (600 ppm/30 min) and treated with hyaluronidase, which degrades HA. Characteristic blot, which was repeated five times. C: human airway smooth muscle cells (hASMC) membrane potential (Vm) measurements 24 h postincubation with 150 μg/mL LMW-HA generated as described in B. Individual values and means ± 1 SE; n = 20 each; Student’s t test. D: measurement of [Ca2+]i [as the ratio of unbound Fura2 (340 nm) to bound Fura2 (380 nm)] of hASMC loaded with Fura2 following the addition of LMW-HA or saline (indicated by the arrow). The initial fast increase in [Ca2+]i peak was followed by a decrease to a lower steady-state value that lasted as long as LMW-HA was present in the recording chamber. Individual values and means ± 1 SE; n = 8 for each condition.
Article Snippet: Lane 1 : HA Mega-HA Ladder (Hyalose); lane 2 : select-HA Hiladder; lane 3 : Yabro; lane 4 : Yabro exposed to Br 2 (400 ppm for 30 min) and stored at 4°C for 1 or 24 h. B , right : agar gel electrophoresis of concentrated BALF from air and Br 2 -exposed mice.
Techniques: Enzyme-linked Immunosorbent Assay, Nucleic Acid Electrophoresis, Molecular Weight, Generated
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![A: concentrations of hyaluronan (HA) in bronchoalveolar lavage fluid (BALF) at 1 and 24 h postexposure to Br2 (600 ppm for 30 min), measured by ELISA. Individual values and means ± 1 SE; number of mice for each condition as follows: air = 8; 1 h and 24 h postexposure to Br2 = 9 each. One-way analysis of variance was performed followed by the Tukey t test. B, left: agar gel electrophoresis of Yabro (a form of high-molecular-weight hyaluronan; 3 mg/mL) before and following exposure to Br2. Lane 1: HA <t>Mega-HA</t> <t>Ladder</t> <t>(Hyalose);</t> lane 2: select-HA Hiladder; lane 3: Yabro; lane 4: Yabro exposed to Br2 (400 ppm for 30 min) and stored at 4°C for 1 or 24 h. B, right: agar gel electrophoresis of concentrated BALF from air and Br2-exposed mice. Lane 1: select-HA Hiladder; Lane 2: select-HA LoLadder. The different lanes show the appearance of BALF at the indicated times postexposure. For the last lane, BALF was obtained at 24 h postexposure to Br2 (600 ppm/30 min) and treated with hyaluronidase, which degrades HA. Characteristic blot, which was repeated five times. C: human airway smooth muscle cells (hASMC) membrane potential (Vm) measurements 24 h postincubation with 150 μg/mL LMW-HA generated as described in B. Individual values and means ± 1 SE; n = 20 each; Student’s t test. D: measurement of [Ca2+]i [as the ratio of unbound Fura2 (340 nm) to bound Fura2 (380 nm)] of hASMC loaded with Fura2 following the addition of LMW-HA or saline (indicated by the arrow). The initial fast increase in [Ca2+]i peak was followed by a decrease to a lower steady-state value that lasted as long as LMW-HA was present in the recording chamber. Individual values and means ± 1 SE; n = 8 for each condition.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_9432/pmc07099432/pmc07099432__zh50022077760006.jpg)