Journal: Toxins

Article Title: Chimeric Peptides from Californiconus californicus and Heterodontus francisci with Antigen-Binding Capacity: A Conotoxin Scaffold to Create Non-Natural Antibodies (NoNaBodies)

doi: 10.3390/toxins15040269

Figure Lengend Snippet: Three-dimensional in vitro angiogenesis assay based on collagen gel-embedded endothelial cell spheroids. Human umbilical vein endothelial cell (HUVEC) spheroids treated with VEGF 165 , anti-VEGF 165 VNAR P98Y, and the chimeric protein cal_P98Y. ( a ) HUVEC spheroid without treatment (control basal medium (BM), p < 0.01 (***)). ( b ) Spheroid stimulated with VEGF 165 (EC + VEGF 165 ). ( c ) Positive control, spheroids stimulated with VEGF 165 and treated with VNAR P98Y (EC + VEGF 165 + VNAR P98Y). ( d ) Endothelial cell spheroids stimulated with VEGF 165 and treated with cal14.1a (EC + VEGF 165 + cal14.1a) p < 0.01 (***). ( e ) Endothelial cell spheroids stimulated with VEGF 165 and treated with cal_P98Y (EC + VEGF 165 + cal_P98Y). ( f ) A plot of cumulative sprout lengths (CSL) of capillary-like sprouts measured after 24 h of incubation. Angiogenesis sprouts were significantly ( p < 0.001 (****)) inhibited when the spheroids were treated with VNAR P98Y and isolated chimeric protein cal_P98Y.

Article Snippet: Human umbilical vein endothelial cells (HUVEC) were cultured in endothelial cell growth medium (ECGM; Cell Applications, Inc., San Diego, CA, USA) and incubated at 37 °C with 5% CO 2 .

Techniques: In Vitro, Angiogenesis Assay, Positive Control, Incubation, Isolation

Journal: Foods

Article Title: Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits

doi: 10.3390/foods12071505

Figure Lengend Snippet: Inhibitory capacity of the blackberry extracts (BB) (5, 10 and 20 mg/mL) and quercetin 25 μM (Q) of intracellular malondialdehyde (MDA) generation as a marker of lipid oxidation induced by TBH. C− are standard HUVEC cells. C+ are HUVEC cells in contact with TBH but without treatment. Results expressed as the average nmol MDA/mL ± SD (n = 6). Groups with different letters are significantly different ( p < 0.05).

Article Snippet: HUVEC cell lines were obtained from Cell Applications Inc. (San Diego, CA, USA).

Techniques: Marker

Journal: Pharmaceutics

Article Title: Mesenchymal Stem/Stromal Cells in Skeletal Muscle Are Pro-Angiogenic, and the Effect Is Potentiated by Erythropoietin

doi: 10.3390/pharmaceutics15041049

Figure Lengend Snippet: Proliferation of cultured mMSCs by Erythropoietin (Epo). ( a ) Left, Epo-R (green) expression in mMSCs. Nuclei were stained with DAPI (blue). NC, negative control. Bar = 50 µm. Right, western blot analysis. Epo stimulated the phosphorylation of Akt and STAT3 in the mMSCs. Epo-R, erythropoietin receptor. ( b ) Efficacy of Epo stimulation on the propagation of HUVECs, skMCs, and mMSCs. N = 3 to 6. # p < 0.01. HUVECs, human umbilical cord vein endothelial cells.

Article Snippet: Rat skeletal muscle cells (skMCs) and human umbilical cord vein endothelial cells (HUVECs) were purchased from Cell Applications Inc. (San Diego, CA, USA).

Techniques: Cell Culture, Expressing, Staining, Negative Control, Western Blot