Structured Review

Cell Applications Inc huvecs
SEM of the luminal surface of HUA and <t>sDHUAs</t> seeded with <t>HUVECs.</t> ( A , B ) HUA, ( C – F ) sDHUA, 204 W, 4 h, ( G – J ) sDHUA, 204 W, 8 h, ( K – N ) sDHUA, 285 W, 2 h, ( O – R ) sDHUA, 285 W, 4 h. ( A , C , G , K , O ) Magnification: 2000×, Scale bar = 10 μm; ( B , D , H , L , P ) Magnification: 10,000×, Scale bar = 1 μm; ( E , I , M , Q ) Magnification: 800×, Scale bar = 10 μm; ( F , J , N , R ) Magnification: 2000×, Scale bar = 10 μm.
Huvecs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/huvecs/product/Cell Applications Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
huvecs - by Bioz Stars, 2022-07
86/100 stars

Images

1) Product Images from "Sonication-Assisted Method for Decellularization of Human Umbilical Artery for Small-Caliber Vascular Tissue Engineering"

Article Title: Sonication-Assisted Method for Decellularization of Human Umbilical Artery for Small-Caliber Vascular Tissue Engineering

Journal: Polymers

doi: 10.3390/polym13111699

SEM of the luminal surface of HUA and sDHUAs seeded with HUVECs. ( A , B ) HUA, ( C – F ) sDHUA, 204 W, 4 h, ( G – J ) sDHUA, 204 W, 8 h, ( K – N ) sDHUA, 285 W, 2 h, ( O – R ) sDHUA, 285 W, 4 h. ( A , C , G , K , O ) Magnification: 2000×, Scale bar = 10 μm; ( B , D , H , L , P ) Magnification: 10,000×, Scale bar = 1 μm; ( E , I , M , Q ) Magnification: 800×, Scale bar = 10 μm; ( F , J , N , R ) Magnification: 2000×, Scale bar = 10 μm.
Figure Legend Snippet: SEM of the luminal surface of HUA and sDHUAs seeded with HUVECs. ( A , B ) HUA, ( C – F ) sDHUA, 204 W, 4 h, ( G – J ) sDHUA, 204 W, 8 h, ( K – N ) sDHUA, 285 W, 2 h, ( O – R ) sDHUA, 285 W, 4 h. ( A , C , G , K , O ) Magnification: 2000×, Scale bar = 10 μm; ( B , D , H , L , P ) Magnification: 10,000×, Scale bar = 1 μm; ( E , I , M , Q ) Magnification: 800×, Scale bar = 10 μm; ( F , J , N , R ) Magnification: 2000×, Scale bar = 10 μm.

Techniques Used:

2) Product Images from "Manipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells"

Article Title: Manipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells

Journal: Cell Research

doi: 10.1038/cr.2014.59

Successful engraftment of generated hPSC-derived EPs to form functional vessels in vivo . (A) Schematic of our finalized approach for generation of hPSC-derived EPs (VEC + CD31 + CD34 + CD14 − ). This is comprised of phase 1 and 2 in only 6 days, and sequential application of the following molecules: BMP4/GSK-3βI in phase 1 and VEGF-A/DAPT in phase 2. (B) Comparison of cell numbers of hPSCs before differentiation, generated EPs (day 6), and VEC + CD31 + ECs (day 14) after phase 3 expansion using our differentiation protocol. (C) Matrigel (MG) plugs with hPSC-derived EPs subcutaneously transplanted into NOD/SCID mice. In vivo live images of capillary vessel formation generated by the hPSC-derived VEC-EGFP + EPs 2 weeks after transplantation. BF, bright field. Scale bars, 100 μm (bottom) and 200 μm (top). (D) The MG plug with hPSC-derived EPs were harvested 4 weeks after transplantation (top), fixed in 2% paraformaldehyde (bottom, left), and subjected to fluorescent whole-mount immunostaining. The bottom-right panel shows anastomosis (arrowhead) between the neovessel formed by transplanted EGFP + EPs and a host vessel of NOD/SCID mice expressing murine VE-cadherin (red). Scale bars, 500 μm (top) and 200 μm (bottom, right). (E) Fluorescent immunostaining of cryosections of the MG plug in D shows anastomoses (arrowheads) between the neovessel formed by transplanted EGFP + EPs and host capillary vessels of NOD/SCID mice expressing murine VE-cadherin (red). Scale bar, 20 μm. (F) The neovessels formed by transplanted EGFP + EPs in a Matrigel plug with functional anastomosis (arrowhead) to native vessels were labeled with lectin (GS-IB 4 , blue). Scale bar, 20 μm. (G) In vivo live images of EGFP + EP-derived capillary vessels 3 months after transplantation. Black arrowheads indicate host angiogenic sprouting capillary vessels, whereas white arrowheads indicate neovessels formed by transplanted EPs in connection with host sprouting vessels. Scale bars, 100 μm. (H) The transplanted cell numbers of EPs (VEC + CD31 + CD34 + CD14 − ) sorted on day 6, ECs (VEC + CD31 + ) sorted on day 14 or VEC-EGFP + HUVECs were positively correlated with quantitative total tube area in in vivo Matrigel plugs. EPs have a three- to fivefold efficacy of quantitative vascular tube formation in vivo , compared with ECs or HUVECs. * P
Figure Legend Snippet: Successful engraftment of generated hPSC-derived EPs to form functional vessels in vivo . (A) Schematic of our finalized approach for generation of hPSC-derived EPs (VEC + CD31 + CD34 + CD14 − ). This is comprised of phase 1 and 2 in only 6 days, and sequential application of the following molecules: BMP4/GSK-3βI in phase 1 and VEGF-A/DAPT in phase 2. (B) Comparison of cell numbers of hPSCs before differentiation, generated EPs (day 6), and VEC + CD31 + ECs (day 14) after phase 3 expansion using our differentiation protocol. (C) Matrigel (MG) plugs with hPSC-derived EPs subcutaneously transplanted into NOD/SCID mice. In vivo live images of capillary vessel formation generated by the hPSC-derived VEC-EGFP + EPs 2 weeks after transplantation. BF, bright field. Scale bars, 100 μm (bottom) and 200 μm (top). (D) The MG plug with hPSC-derived EPs were harvested 4 weeks after transplantation (top), fixed in 2% paraformaldehyde (bottom, left), and subjected to fluorescent whole-mount immunostaining. The bottom-right panel shows anastomosis (arrowhead) between the neovessel formed by transplanted EGFP + EPs and a host vessel of NOD/SCID mice expressing murine VE-cadherin (red). Scale bars, 500 μm (top) and 200 μm (bottom, right). (E) Fluorescent immunostaining of cryosections of the MG plug in D shows anastomoses (arrowheads) between the neovessel formed by transplanted EGFP + EPs and host capillary vessels of NOD/SCID mice expressing murine VE-cadherin (red). Scale bar, 20 μm. (F) The neovessels formed by transplanted EGFP + EPs in a Matrigel plug with functional anastomosis (arrowhead) to native vessels were labeled with lectin (GS-IB 4 , blue). Scale bar, 20 μm. (G) In vivo live images of EGFP + EP-derived capillary vessels 3 months after transplantation. Black arrowheads indicate host angiogenic sprouting capillary vessels, whereas white arrowheads indicate neovessels formed by transplanted EPs in connection with host sprouting vessels. Scale bars, 100 μm. (H) The transplanted cell numbers of EPs (VEC + CD31 + CD34 + CD14 − ) sorted on day 6, ECs (VEC + CD31 + ) sorted on day 14 or VEC-EGFP + HUVECs were positively correlated with quantitative total tube area in in vivo Matrigel plugs. EPs have a three- to fivefold efficacy of quantitative vascular tube formation in vivo , compared with ECs or HUVECs. * P

Techniques Used: Generated, Derivative Assay, Functional Assay, In Vivo, Mouse Assay, Transplantation Assay, Immunostaining, Expressing, Labeling

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Cell Applications Inc hdl huvecs
    <t>HDL</t> attenuates dextrose-induced inhibition of ERK activation. <t>HUVECs</t> were incubated with increasing dextrose concentrations (5.7–60 mM) for 15 min ( a ), or HDL (5–120 µg/mL) ( b ), *p
    Hdl Huvecs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hdl huvecs/product/Cell Applications Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hdl huvecs - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

    86
    Cell Applications Inc huvecs
    Successful engraftment of generated <t>hPSC-derived</t> EPs to form functional vessels in vivo . (A) Schematic of our finalized approach for generation of hPSC-derived EPs (VEC + CD31 + CD34 + CD14 − ). This is comprised of phase 1 and 2 in only 6 days, and sequential application of the following molecules: BMP4/GSK-3βI in phase 1 and VEGF-A/DAPT in phase 2. (B) Comparison of cell numbers of hPSCs before differentiation, generated EPs (day 6), and VEC + CD31 + ECs (day 14) after phase 3 expansion using our differentiation protocol. (C) Matrigel (MG) plugs with hPSC-derived EPs subcutaneously transplanted into NOD/SCID mice. In vivo live images of capillary vessel formation generated by the hPSC-derived VEC-EGFP + EPs 2 weeks after transplantation. BF, bright field. Scale bars, 100 μm (bottom) and 200 μm (top). (D) The MG plug with hPSC-derived EPs were harvested 4 weeks after transplantation (top), fixed in 2% paraformaldehyde (bottom, left), and subjected to fluorescent whole-mount immunostaining. The bottom-right panel shows anastomosis (arrowhead) between the neovessel formed by transplanted EGFP + EPs and a host vessel of NOD/SCID mice expressing murine VE-cadherin (red). Scale bars, 500 μm (top) and 200 μm (bottom, right). (E) Fluorescent immunostaining of cryosections of the MG plug in D shows anastomoses (arrowheads) between the neovessel formed by transplanted EGFP + EPs and host capillary vessels of NOD/SCID mice expressing murine VE-cadherin (red). Scale bar, 20 μm. (F) The neovessels formed by transplanted EGFP + EPs in a Matrigel plug with functional anastomosis (arrowhead) to native vessels were labeled with lectin (GS-IB 4 , blue). Scale bar, 20 μm. (G) In vivo live images of EGFP + EP-derived capillary vessels 3 months after transplantation. Black arrowheads indicate host angiogenic sprouting capillary vessels, whereas white arrowheads indicate neovessels formed by transplanted EPs in connection with host sprouting vessels. Scale bars, 100 μm. (H) The transplanted cell numbers of EPs (VEC + CD31 + CD34 + CD14 − ) sorted on day 6, ECs (VEC + CD31 + ) sorted on day 14 or VEC-EGFP + <t>HUVECs</t> were positively correlated with quantitative total tube area in in vivo Matrigel plugs. EPs have a three- to fivefold efficacy of quantitative vascular tube formation in vivo , compared with ECs or HUVECs. * P
    Huvecs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/huvecs/product/Cell Applications Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    huvecs - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

    86
    Cell Applications Inc shear stress experiments huvecs
    Successful engraftment of generated <t>hPSC-derived</t> EPs to form functional vessels in vivo . (A) Schematic of our finalized approach for generation of hPSC-derived EPs (VEC + CD31 + CD34 + CD14 − ). This is comprised of phase 1 and 2 in only 6 days, and sequential application of the following molecules: BMP4/GSK-3βI in phase 1 and VEGF-A/DAPT in phase 2. (B) Comparison of cell numbers of hPSCs before differentiation, generated EPs (day 6), and VEC + CD31 + ECs (day 14) after phase 3 expansion using our differentiation protocol. (C) Matrigel (MG) plugs with hPSC-derived EPs subcutaneously transplanted into NOD/SCID mice. In vivo live images of capillary vessel formation generated by the hPSC-derived VEC-EGFP + EPs 2 weeks after transplantation. BF, bright field. Scale bars, 100 μm (bottom) and 200 μm (top). (D) The MG plug with hPSC-derived EPs were harvested 4 weeks after transplantation (top), fixed in 2% paraformaldehyde (bottom, left), and subjected to fluorescent whole-mount immunostaining. The bottom-right panel shows anastomosis (arrowhead) between the neovessel formed by transplanted EGFP + EPs and a host vessel of NOD/SCID mice expressing murine VE-cadherin (red). Scale bars, 500 μm (top) and 200 μm (bottom, right). (E) Fluorescent immunostaining of cryosections of the MG plug in D shows anastomoses (arrowheads) between the neovessel formed by transplanted EGFP + EPs and host capillary vessels of NOD/SCID mice expressing murine VE-cadherin (red). Scale bar, 20 μm. (F) The neovessels formed by transplanted EGFP + EPs in a Matrigel plug with functional anastomosis (arrowhead) to native vessels were labeled with lectin (GS-IB 4 , blue). Scale bar, 20 μm. (G) In vivo live images of EGFP + EP-derived capillary vessels 3 months after transplantation. Black arrowheads indicate host angiogenic sprouting capillary vessels, whereas white arrowheads indicate neovessels formed by transplanted EPs in connection with host sprouting vessels. Scale bars, 100 μm. (H) The transplanted cell numbers of EPs (VEC + CD31 + CD34 + CD14 − ) sorted on day 6, ECs (VEC + CD31 + ) sorted on day 14 or VEC-EGFP + <t>HUVECs</t> were positively correlated with quantitative total tube area in in vivo Matrigel plugs. EPs have a three- to fivefold efficacy of quantitative vascular tube formation in vivo , compared with ECs or HUVECs. * P
    Shear Stress Experiments Huvecs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shear stress experiments huvecs/product/Cell Applications Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    shear stress experiments huvecs - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

    86
    Cell Applications Inc human umbilical venous endothelial cells huvecs
    Successful engraftment of generated <t>hPSC-derived</t> EPs to form functional vessels in vivo . (A) Schematic of our finalized approach for generation of hPSC-derived EPs (VEC + CD31 + CD34 + CD14 − ). This is comprised of phase 1 and 2 in only 6 days, and sequential application of the following molecules: BMP4/GSK-3βI in phase 1 and VEGF-A/DAPT in phase 2. (B) Comparison of cell numbers of hPSCs before differentiation, generated EPs (day 6), and VEC + CD31 + ECs (day 14) after phase 3 expansion using our differentiation protocol. (C) Matrigel (MG) plugs with hPSC-derived EPs subcutaneously transplanted into NOD/SCID mice. In vivo live images of capillary vessel formation generated by the hPSC-derived VEC-EGFP + EPs 2 weeks after transplantation. BF, bright field. Scale bars, 100 μm (bottom) and 200 μm (top). (D) The MG plug with hPSC-derived EPs were harvested 4 weeks after transplantation (top), fixed in 2% paraformaldehyde (bottom, left), and subjected to fluorescent whole-mount immunostaining. The bottom-right panel shows anastomosis (arrowhead) between the neovessel formed by transplanted EGFP + EPs and a host vessel of NOD/SCID mice expressing murine VE-cadherin (red). Scale bars, 500 μm (top) and 200 μm (bottom, right). (E) Fluorescent immunostaining of cryosections of the MG plug in D shows anastomoses (arrowheads) between the neovessel formed by transplanted EGFP + EPs and host capillary vessels of NOD/SCID mice expressing murine VE-cadherin (red). Scale bar, 20 μm. (F) The neovessels formed by transplanted EGFP + EPs in a Matrigel plug with functional anastomosis (arrowhead) to native vessels were labeled with lectin (GS-IB 4 , blue). Scale bar, 20 μm. (G) In vivo live images of EGFP + EP-derived capillary vessels 3 months after transplantation. Black arrowheads indicate host angiogenic sprouting capillary vessels, whereas white arrowheads indicate neovessels formed by transplanted EPs in connection with host sprouting vessels. Scale bars, 100 μm. (H) The transplanted cell numbers of EPs (VEC + CD31 + CD34 + CD14 − ) sorted on day 6, ECs (VEC + CD31 + ) sorted on day 14 or VEC-EGFP + <t>HUVECs</t> were positively correlated with quantitative total tube area in in vivo Matrigel plugs. EPs have a three- to fivefold efficacy of quantitative vascular tube formation in vivo , compared with ECs or HUVECs. * P
    Human Umbilical Venous Endothelial Cells Huvecs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human umbilical venous endothelial cells huvecs/product/Cell Applications Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human umbilical venous endothelial cells huvecs - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    HDL attenuates dextrose-induced inhibition of ERK activation. HUVECs were incubated with increasing dextrose concentrations (5.7–60 mM) for 15 min ( a ), or HDL (5–120 µg/mL) ( b ), *p

    Journal: Cardiovascular Diabetology

    Article Title: High-density lipoproteins attenuate high glucose-impaired endothelial cell signaling and functions: potential implications for improved vascular repair in diabetes

    doi: 10.1186/s12933-017-0605-8

    Figure Lengend Snippet: HDL attenuates dextrose-induced inhibition of ERK activation. HUVECs were incubated with increasing dextrose concentrations (5.7–60 mM) for 15 min ( a ), or HDL (5–120 µg/mL) ( b ), *p

    Article Snippet: Cell isolation, culture and preparation of HDL HUVECs (Cell Applications Inc., San Diego, CA, USA) were maintained in MCDB (Molecular, Cellular, and Developmental Biology) medium containing 15% fetal bovine serum (FBS, lipoprotein deficient), 0.009% heparin, 0.015% endothelial cell growth supplement (Cat.

    Techniques: Inhibition, Activation Assay, Incubation

    HDL attenuates dextrose-induced inhibition of p38 activation. HUVECs were incubated with increasing dextrose concentrations (5.7–60 mM) for 15 min ( a ) or HDL (5–120 µg/mL) ( b ), *p

    Journal: Cardiovascular Diabetology

    Article Title: High-density lipoproteins attenuate high glucose-impaired endothelial cell signaling and functions: potential implications for improved vascular repair in diabetes

    doi: 10.1186/s12933-017-0605-8

    Figure Lengend Snippet: HDL attenuates dextrose-induced inhibition of p38 activation. HUVECs were incubated with increasing dextrose concentrations (5.7–60 mM) for 15 min ( a ) or HDL (5–120 µg/mL) ( b ), *p

    Article Snippet: Cell isolation, culture and preparation of HDL HUVECs (Cell Applications Inc., San Diego, CA, USA) were maintained in MCDB (Molecular, Cellular, and Developmental Biology) medium containing 15% fetal bovine serum (FBS, lipoprotein deficient), 0.009% heparin, 0.015% endothelial cell growth supplement (Cat.

    Techniques: Inhibition, Activation Assay, Incubation

    HDL rescues dextrose-induced inhibition of HUVEC migration. HUVEC migration was determined using transwell membranes. HUVECs were seeded on the upper chamber, serum-deprived for 12 h, then the transwells were placed into the lower chamber containing increasing dextrose concentrations (5.7–40 mM) and/or HDL (80 μg/mL) and incubated overnight. The cells that migrated through the membrane were fixed and stained by DAPI. Upper panels are representative images of membranes of migrated cells. Data shown are the mean ± SEM of results performed in triplicate from 3 independent experiments. # p

    Journal: Cardiovascular Diabetology

    Article Title: High-density lipoproteins attenuate high glucose-impaired endothelial cell signaling and functions: potential implications for improved vascular repair in diabetes

    doi: 10.1186/s12933-017-0605-8

    Figure Lengend Snippet: HDL rescues dextrose-induced inhibition of HUVEC migration. HUVEC migration was determined using transwell membranes. HUVECs were seeded on the upper chamber, serum-deprived for 12 h, then the transwells were placed into the lower chamber containing increasing dextrose concentrations (5.7–40 mM) and/or HDL (80 μg/mL) and incubated overnight. The cells that migrated through the membrane were fixed and stained by DAPI. Upper panels are representative images of membranes of migrated cells. Data shown are the mean ± SEM of results performed in triplicate from 3 independent experiments. # p

    Article Snippet: Cell isolation, culture and preparation of HDL HUVECs (Cell Applications Inc., San Diego, CA, USA) were maintained in MCDB (Molecular, Cellular, and Developmental Biology) medium containing 15% fetal bovine serum (FBS, lipoprotein deficient), 0.009% heparin, 0.015% endothelial cell growth supplement (Cat.

    Techniques: Inhibition, Migration, Incubation, Staining

    HDL attenuates dextrose-induced inhibition of HUVEC proliferation. a Cell proliferation was determined after cells were incubated with dextrose (5.7, 20 and 40 mM) or dextrose plus HDL (80 µg/mL) for 96 h using BrdU incorporation. 2.5% FBS (control) and 15% FBS (positive control) treatments were also included. b Protein levels of PCNA were determined in HUVEC lysates incubated with dextrose (5.7, 20 and 40 mM) or dextrose plus HDL (80 µg/mL) for 72 h using Western blotting. β-Actin was used as the protein loading control. Data shown are the mean ± SEM of results from three independent experiments. # p

    Journal: Cardiovascular Diabetology

    Article Title: High-density lipoproteins attenuate high glucose-impaired endothelial cell signaling and functions: potential implications for improved vascular repair in diabetes

    doi: 10.1186/s12933-017-0605-8

    Figure Lengend Snippet: HDL attenuates dextrose-induced inhibition of HUVEC proliferation. a Cell proliferation was determined after cells were incubated with dextrose (5.7, 20 and 40 mM) or dextrose plus HDL (80 µg/mL) for 96 h using BrdU incorporation. 2.5% FBS (control) and 15% FBS (positive control) treatments were also included. b Protein levels of PCNA were determined in HUVEC lysates incubated with dextrose (5.7, 20 and 40 mM) or dextrose plus HDL (80 µg/mL) for 72 h using Western blotting. β-Actin was used as the protein loading control. Data shown are the mean ± SEM of results from three independent experiments. # p

    Article Snippet: Cell isolation, culture and preparation of HDL HUVECs (Cell Applications Inc., San Diego, CA, USA) were maintained in MCDB (Molecular, Cellular, and Developmental Biology) medium containing 15% fetal bovine serum (FBS, lipoprotein deficient), 0.009% heparin, 0.015% endothelial cell growth supplement (Cat.

    Techniques: Inhibition, Incubation, BrdU Incorporation Assay, Positive Control, Western Blot

    HDL mitigates the inhibition of Akt phosphorylation by dextrose. HUVECs were incubated with increasing dextrose concentrations (5.7–60 mM) for 15 min ( a ) or HDL (5–120 µg/mL) ( b ), *p

    Journal: Cardiovascular Diabetology

    Article Title: High-density lipoproteins attenuate high glucose-impaired endothelial cell signaling and functions: potential implications for improved vascular repair in diabetes

    doi: 10.1186/s12933-017-0605-8

    Figure Lengend Snippet: HDL mitigates the inhibition of Akt phosphorylation by dextrose. HUVECs were incubated with increasing dextrose concentrations (5.7–60 mM) for 15 min ( a ) or HDL (5–120 µg/mL) ( b ), *p

    Article Snippet: Cell isolation, culture and preparation of HDL HUVECs (Cell Applications Inc., San Diego, CA, USA) were maintained in MCDB (Molecular, Cellular, and Developmental Biology) medium containing 15% fetal bovine serum (FBS, lipoprotein deficient), 0.009% heparin, 0.015% endothelial cell growth supplement (Cat.

    Techniques: Inhibition, Incubation

    Successful engraftment of generated hPSC-derived EPs to form functional vessels in vivo . (A) Schematic of our finalized approach for generation of hPSC-derived EPs (VEC + CD31 + CD34 + CD14 − ). This is comprised of phase 1 and 2 in only 6 days, and sequential application of the following molecules: BMP4/GSK-3βI in phase 1 and VEGF-A/DAPT in phase 2. (B) Comparison of cell numbers of hPSCs before differentiation, generated EPs (day 6), and VEC + CD31 + ECs (day 14) after phase 3 expansion using our differentiation protocol. (C) Matrigel (MG) plugs with hPSC-derived EPs subcutaneously transplanted into NOD/SCID mice. In vivo live images of capillary vessel formation generated by the hPSC-derived VEC-EGFP + EPs 2 weeks after transplantation. BF, bright field. Scale bars, 100 μm (bottom) and 200 μm (top). (D) The MG plug with hPSC-derived EPs were harvested 4 weeks after transplantation (top), fixed in 2% paraformaldehyde (bottom, left), and subjected to fluorescent whole-mount immunostaining. The bottom-right panel shows anastomosis (arrowhead) between the neovessel formed by transplanted EGFP + EPs and a host vessel of NOD/SCID mice expressing murine VE-cadherin (red). Scale bars, 500 μm (top) and 200 μm (bottom, right). (E) Fluorescent immunostaining of cryosections of the MG plug in D shows anastomoses (arrowheads) between the neovessel formed by transplanted EGFP + EPs and host capillary vessels of NOD/SCID mice expressing murine VE-cadherin (red). Scale bar, 20 μm. (F) The neovessels formed by transplanted EGFP + EPs in a Matrigel plug with functional anastomosis (arrowhead) to native vessels were labeled with lectin (GS-IB 4 , blue). Scale bar, 20 μm. (G) In vivo live images of EGFP + EP-derived capillary vessels 3 months after transplantation. Black arrowheads indicate host angiogenic sprouting capillary vessels, whereas white arrowheads indicate neovessels formed by transplanted EPs in connection with host sprouting vessels. Scale bars, 100 μm. (H) The transplanted cell numbers of EPs (VEC + CD31 + CD34 + CD14 − ) sorted on day 6, ECs (VEC + CD31 + ) sorted on day 14 or VEC-EGFP + HUVECs were positively correlated with quantitative total tube area in in vivo Matrigel plugs. EPs have a three- to fivefold efficacy of quantitative vascular tube formation in vivo , compared with ECs or HUVECs. * P

    Journal: Cell Research

    Article Title: Manipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells

    doi: 10.1038/cr.2014.59

    Figure Lengend Snippet: Successful engraftment of generated hPSC-derived EPs to form functional vessels in vivo . (A) Schematic of our finalized approach for generation of hPSC-derived EPs (VEC + CD31 + CD34 + CD14 − ). This is comprised of phase 1 and 2 in only 6 days, and sequential application of the following molecules: BMP4/GSK-3βI in phase 1 and VEGF-A/DAPT in phase 2. (B) Comparison of cell numbers of hPSCs before differentiation, generated EPs (day 6), and VEC + CD31 + ECs (day 14) after phase 3 expansion using our differentiation protocol. (C) Matrigel (MG) plugs with hPSC-derived EPs subcutaneously transplanted into NOD/SCID mice. In vivo live images of capillary vessel formation generated by the hPSC-derived VEC-EGFP + EPs 2 weeks after transplantation. BF, bright field. Scale bars, 100 μm (bottom) and 200 μm (top). (D) The MG plug with hPSC-derived EPs were harvested 4 weeks after transplantation (top), fixed in 2% paraformaldehyde (bottom, left), and subjected to fluorescent whole-mount immunostaining. The bottom-right panel shows anastomosis (arrowhead) between the neovessel formed by transplanted EGFP + EPs and a host vessel of NOD/SCID mice expressing murine VE-cadherin (red). Scale bars, 500 μm (top) and 200 μm (bottom, right). (E) Fluorescent immunostaining of cryosections of the MG plug in D shows anastomoses (arrowheads) between the neovessel formed by transplanted EGFP + EPs and host capillary vessels of NOD/SCID mice expressing murine VE-cadherin (red). Scale bar, 20 μm. (F) The neovessels formed by transplanted EGFP + EPs in a Matrigel plug with functional anastomosis (arrowhead) to native vessels were labeled with lectin (GS-IB 4 , blue). Scale bar, 20 μm. (G) In vivo live images of EGFP + EP-derived capillary vessels 3 months after transplantation. Black arrowheads indicate host angiogenic sprouting capillary vessels, whereas white arrowheads indicate neovessels formed by transplanted EPs in connection with host sprouting vessels. Scale bars, 100 μm. (H) The transplanted cell numbers of EPs (VEC + CD31 + CD34 + CD14 − ) sorted on day 6, ECs (VEC + CD31 + ) sorted on day 14 or VEC-EGFP + HUVECs were positively correlated with quantitative total tube area in in vivo Matrigel plugs. EPs have a three- to fivefold efficacy of quantitative vascular tube formation in vivo , compared with ECs or HUVECs. * P

    Article Snippet: In vitro vascular tube formation assay 50 μl Matrigel per well was placed into wells of a 96-well plate and incubated at 37 °C for 1 h. 1.5 × 104 of either hPSC-derived endothelial lineage cells sorted by FACS, HUVECs (Cell Applications Inc) or HUCBCs (Harvard University) were suspended in 100 μl of EBM-2 supplemented with 5% FBS and 50 ng/ml VEGF, and then seeded onto the Matrigel plugs.

    Techniques: Generated, Derivative Assay, Functional Assay, In Vivo, Mouse Assay, Transplantation Assay, Immunostaining, Expressing, Labeling