human vegf  (Millipore)


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    Name:
    VEGF human
    Description:
    VEGF vascular endothelial growth factor signals through the three receptors fms like tyrosine kinase flt 1 KDR gene product the murine homolog of KDR is the flk 1 gene product and the flt4 gene product Recombinant human VEGF is a 38 2kDa disulfide linked homodimeric protein consisting of two 165 amino acid polypeptide chains
    Catalog Number:
    srp3182
    Price:
    None
    Applications:
    VEGF human has been used as a chemoattractant for HUVECs (human umbilical vein endothelial cells) migration assay.
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    Structured Review

    Millipore human vegf
    VEGF human
    VEGF vascular endothelial growth factor signals through the three receptors fms like tyrosine kinase flt 1 KDR gene product the murine homolog of KDR is the flk 1 gene product and the flt4 gene product Recombinant human VEGF is a 38 2kDa disulfide linked homodimeric protein consisting of two 165 amino acid polypeptide chains
    https://www.bioz.com/result/human vegf/product/Millipore
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    human vegf - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Microenvironment Changes (in pH) Affect VEGF Alternative Splicing"

    Article Title: Microenvironment Changes (in pH) Affect VEGF Alternative Splicing

    Journal: Cancer Microenvironment

    doi: 10.1007/s12307-008-0013-4

    a SR proteins involved in the control of the VEGF splicing pattern. Most SR proteins were up-regulated in acidic conditions both after 6 and 8 h of exposure. b Expression of the SR protein SRp20, in RL95, increased significantly ( p
    Figure Legend Snippet: a SR proteins involved in the control of the VEGF splicing pattern. Most SR proteins were up-regulated in acidic conditions both after 6 and 8 h of exposure. b Expression of the SR protein SRp20, in RL95, increased significantly ( p

    Techniques Used: Expressing

    VEGF isoforms expression pattern by RL95 cells in response to changes in the microenvironment. By real time RT-PCR ( a ) and ELISA ( b), we can see an increase in VEGF production in acidic and hypoxic (mimicked by CoCl 2 ) conditions. A shift in the VEGF isoforms splicing pattern is more evident at pH 5.5 where VEGF121 expression is significantly different from all other isoforms ( p
    Figure Legend Snippet: VEGF isoforms expression pattern by RL95 cells in response to changes in the microenvironment. By real time RT-PCR ( a ) and ELISA ( b), we can see an increase in VEGF production in acidic and hypoxic (mimicked by CoCl 2 ) conditions. A shift in the VEGF isoforms splicing pattern is more evident at pH 5.5 where VEGF121 expression is significantly different from all other isoforms ( p

    Techniques Used: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    2) Product Images from "Differential Healing After Sirolimus, Paclitaxel and Bare Metal Stent Placement in Combination with PPARγ Agonists: Requirement for mTOR/Akt2 in PPARγ Activation"

    Article Title: Differential Healing After Sirolimus, Paclitaxel and Bare Metal Stent Placement in Combination with PPARγ Agonists: Requirement for mTOR/Akt2 in PPARγ Activation

    Journal:

    doi: 10.1161/CIRCRESAHA.109.200519

    Organ culture for vascular endothelial growth factor (VEGF) from rabbit iliac arteries treated with (A) sirolimus-eluting stents (SES), (B) bare metal stents (BMS), or (C) paclitaxel-eluting stents (PES) without rosiglitazone (RSG) for 14 days (n=4 arteries
    Figure Legend Snippet: Organ culture for vascular endothelial growth factor (VEGF) from rabbit iliac arteries treated with (A) sirolimus-eluting stents (SES), (B) bare metal stents (BMS), or (C) paclitaxel-eluting stents (PES) without rosiglitazone (RSG) for 14 days (n=4 arteries

    Techniques Used: Organ Culture

    3) Product Images from "Spatiotemporal release of BMP-2 and VEGF enhances osteogenic and vasculogenic differentiation of human mesenchymal stem cells and endothelial colony-forming cells co-encapsulated in a patterned hydrogel"

    Article Title: Spatiotemporal release of BMP-2 and VEGF enhances osteogenic and vasculogenic differentiation of human mesenchymal stem cells and endothelial colony-forming cells co-encapsulated in a patterned hydrogel

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    doi: 10.1016/j.jconrel.2015.12.031

    Effect of PEG MW, LG segment length, and L/G ratio in PaLGb-Lc macromer on NG size distribution (a–c) and cumulative BSA release (d–f) in PBS with incubation time. (g) The release kinetics of BMP2 (blue circles) and VEGF (red circles) grafted to P8-I and P12-II NGs, respectively. Figure 2g also shows the release kinetics of BMP2 (blue squares) and VEGF (red squares) directly added to SPELA and GelMA gels, respectively, without NG grafting. The abbreviations P4, P8, and P12 in (a–f) represent NGs made from macromers with PEG MW=4, 8, and 12 kDa and average LG segment length of 8. The abbreviations P4-I, P4-II, and P4-III in (a–f) represent NGs with 4 kDa PEG MW with 75%, 60%, and 50% lactide in LG segments, respectively. Morphology of the dried NGs is shown in the inset SEM images in (a–c). Error bars correspond to means ± 1 SD for n=3.
    Figure Legend Snippet: Effect of PEG MW, LG segment length, and L/G ratio in PaLGb-Lc macromer on NG size distribution (a–c) and cumulative BSA release (d–f) in PBS with incubation time. (g) The release kinetics of BMP2 (blue circles) and VEGF (red circles) grafted to P8-I and P12-II NGs, respectively. Figure 2g also shows the release kinetics of BMP2 (blue squares) and VEGF (red squares) directly added to SPELA and GelMA gels, respectively, without NG grafting. The abbreviations P4, P8, and P12 in (a–f) represent NGs made from macromers with PEG MW=4, 8, and 12 kDa and average LG segment length of 8. The abbreviations P4-I, P4-II, and P4-III in (a–f) represent NGs with 4 kDa PEG MW with 75%, 60%, and 50% lactide in LG segments, respectively. Morphology of the dried NGs is shown in the inset SEM images in (a–c). Error bars correspond to means ± 1 SD for n=3.

    Techniques Used: Incubation, Next-Generation Sequencing

    4) Product Images from "Induction of Human Lung Mast Cell Apoptosis by Granule Permeabilization: A Novel Approach for Targeting Mast Cells"

    Article Title: Induction of Human Lung Mast Cell Apoptosis by Granule Permeabilization: A Novel Approach for Targeting Mast Cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2017.01645

    Treatment of human lung biopsies with mefloquine and/or siramesine reduces the levels of VEGF and IL-6. Human lung biopsies were incubated with 20 µM of mefloquine or siramesine or phosphate-buffered saline (PBS) for 6 h. Levels of (A) human VEGF and (B) IL-6 were measured in the supernatants by ELISA ( n = 3; representative of two independent experiments/two donors). Results are presented as mean ± SEM (* P
    Figure Legend Snippet: Treatment of human lung biopsies with mefloquine and/or siramesine reduces the levels of VEGF and IL-6. Human lung biopsies were incubated with 20 µM of mefloquine or siramesine or phosphate-buffered saline (PBS) for 6 h. Levels of (A) human VEGF and (B) IL-6 were measured in the supernatants by ELISA ( n = 3; representative of two independent experiments/two donors). Results are presented as mean ± SEM (* P

    Techniques Used: Incubation, Enzyme-linked Immunosorbent Assay

    5) Product Images from "Immunogene therapy of tumors with vaccine based onXenopus homologous vascular endothelial growth factor as a model antigen"

    Article Title: Immunogene therapy of tumors with vaccine based onXenopus homologous vascular endothelial growth factor as a model antigen

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.191112198

    The identification of VEGF-specific antoantibody in sera in Western blot analysis. Recombinant Xenopus VEGF, VEGF 120, 164, 188, and 165 as well as other growth factors (bFGF and PlGF) were stained with sera isolated from mice immunized with XVEGF-p and other control sera. The positive bands for VEGF can be recognized with the sera isolated from mice immunized with XVEGF-p ( A ), but negative staining from mice immunized with MVEGF-p ( B ) or other control groups. ( C ) Positive staining for bFGF and PlGF was found with the antibodies against bFGF and PlGF, respectively, as positive controls for A , but ( D ) negative staining was found with control IgG.
    Figure Legend Snippet: The identification of VEGF-specific antoantibody in sera in Western blot analysis. Recombinant Xenopus VEGF, VEGF 120, 164, 188, and 165 as well as other growth factors (bFGF and PlGF) were stained with sera isolated from mice immunized with XVEGF-p and other control sera. The positive bands for VEGF can be recognized with the sera isolated from mice immunized with XVEGF-p ( A ), but negative staining from mice immunized with MVEGF-p ( B ) or other control groups. ( C ) Positive staining for bFGF and PlGF was found with the antibodies against bFGF and PlGF, respectively, as positive controls for A , but ( D ) negative staining was found with control IgG.

    Techniques Used: Western Blot, Recombinant, Staining, Isolation, Mouse Assay, Negative Staining

    Inhibition of VEGF-mediated endothelial cell proliferation in vitro and the antitumor effect by the adoptive transfer of Igs in vivo . ( A ) Human umbilical vein endothelial cells were incubated with mouse or human VEGF (300 ng/ml) in the presence of various concentrations of immunoglobulins. Treatment with immunoglobulins from mice immunized with XVEGF-p (■) resulted in the apparent inhibition of endothelial cell proliferation, compared with mice immunized with MVEGF-p (○) or c-p (●) or with nonimmunized mice (▴). But it had no effect on bFGF-mediated endothelial cell proliferation (data not shown). ( B ) Adoptive transfer of immunoglobulins in vivo . The protective antitumor effect against Meth A cells was tested with purified immunoglobulins (25 mg/kg) from mice immunized with XVEGF-p (●), MVEGF-p (○), or c-p (■) or from nonimmunized mice (▴). Results are expressed as means ± SEM. Asterisks (*) indicate a significant difference in tumor volume ( P
    Figure Legend Snippet: Inhibition of VEGF-mediated endothelial cell proliferation in vitro and the antitumor effect by the adoptive transfer of Igs in vivo . ( A ) Human umbilical vein endothelial cells were incubated with mouse or human VEGF (300 ng/ml) in the presence of various concentrations of immunoglobulins. Treatment with immunoglobulins from mice immunized with XVEGF-p (■) resulted in the apparent inhibition of endothelial cell proliferation, compared with mice immunized with MVEGF-p (○) or c-p (●) or with nonimmunized mice (▴). But it had no effect on bFGF-mediated endothelial cell proliferation (data not shown). ( B ) Adoptive transfer of immunoglobulins in vivo . The protective antitumor effect against Meth A cells was tested with purified immunoglobulins (25 mg/kg) from mice immunized with XVEGF-p (●), MVEGF-p (○), or c-p (■) or from nonimmunized mice (▴). Results are expressed as means ± SEM. Asterisks (*) indicate a significant difference in tumor volume ( P

    Techniques Used: Inhibition, In Vitro, Adoptive Transfer Assay, In Vivo, Incubation, Mouse Assay, Purification

    6) Product Images from "Material and regenerative properties of an osteon-mimetic cortical bone-like scaffold"

    Article Title: Material and regenerative properties of an osteon-mimetic cortical bone-like scaffold

    Journal: Regenerative Biomaterials

    doi: 10.1093/rb/rbz008

    DNA Content ( a ), ALP activity ( b ), calcium content ( c ), mRNA expression level of osteogenic markers Runx2 ( d ), OC ( e ) and Col I ( f ) for the cortical scaffolds with hMSC/BMP2-NGs in the lumen and MSC+EFCF/VEGF-NGs on the outer surface and cultured in the perfusion bioreactor. Groups included cortical scaffolds with cells and with growth factors in static culture (G1, blue curve), scaffolds with cells but without growth factors in perfusion culture (G2, light blue curve), and scaffolds with cells and with growth factors in perfusion culture as the experimental group (G3, red). A star in the figures indicates a statistically significant difference between G3 and other groups. Error bars correspond to means ± 1 SD for n = 3
    Figure Legend Snippet: DNA Content ( a ), ALP activity ( b ), calcium content ( c ), mRNA expression level of osteogenic markers Runx2 ( d ), OC ( e ) and Col I ( f ) for the cortical scaffolds with hMSC/BMP2-NGs in the lumen and MSC+EFCF/VEGF-NGs on the outer surface and cultured in the perfusion bioreactor. Groups included cortical scaffolds with cells and with growth factors in static culture (G1, blue curve), scaffolds with cells but without growth factors in perfusion culture (G2, light blue curve), and scaffolds with cells and with growth factors in perfusion culture as the experimental group (G3, red). A star in the figures indicates a statistically significant difference between G3 and other groups. Error bars correspond to means ± 1 SD for n = 3

    Techniques Used: ALP Assay, Activity Assay, Expressing, Next-Generation Sequencing, Cell Culture

    The mRNA expression level of vasculogenic markers VE-cadherin ( a ), vWF ( b ) and CD31 ( c ), Western-blot bands ( d ) and expression level ( e ) of CD31 protein for the cortical scaffolds with hMSC/BMP2-NGs in the lumen and MSC+EFCF/VEGF-NGs on the outer surface and cultured in the perfusion bioreactor. Groups included cortical scaffolds with cells and with growth factors in static culture (G1, blue curve), scaffolds with cells but without growth factors in perfusion culture (G2, light blue curve), and scaffolds with cells and with growth factors in perfusion culture as the experimental group (G3, red). A star in the figures indicates a statistically significant difference between G3 and other groups. Error bars correspond to means ± 1 SD for n = 3
    Figure Legend Snippet: The mRNA expression level of vasculogenic markers VE-cadherin ( a ), vWF ( b ) and CD31 ( c ), Western-blot bands ( d ) and expression level ( e ) of CD31 protein for the cortical scaffolds with hMSC/BMP2-NGs in the lumen and MSC+EFCF/VEGF-NGs on the outer surface and cultured in the perfusion bioreactor. Groups included cortical scaffolds with cells and with growth factors in static culture (G1, blue curve), scaffolds with cells but without growth factors in perfusion culture (G2, light blue curve), and scaffolds with cells and with growth factors in perfusion culture as the experimental group (G3, red). A star in the figures indicates a statistically significant difference between G3 and other groups. Error bars correspond to means ± 1 SD for n = 3

    Techniques Used: Expressing, Western Blot, Next-Generation Sequencing, Cell Culture

    7) Product Images from "The anti-angiogenic and cytotoxic effects of the boswellic acid analog BA145 are potentiated by autophagy inhibitors"

    Article Title: The anti-angiogenic and cytotoxic effects of the boswellic acid analog BA145 are potentiated by autophagy inhibitors

    Journal: Molecular Cancer

    doi: 10.1186/1476-4598-14-6

    Autophagy inhibition potentiates the anti-angiogenic effects of BA145 in vivo. (A) Effect of BA145 and CQ on VEGF induced angiogenesis. Six weeks old C57BL/6 J mice were injected with Matrigel containing BA145 (50 mg/kg), CQ (50 mg/kg), or both BA145 and CQ along with VEGF (150 ng) into the ventral area (6 mice per group). Matrigel plugs were monitored 10 days later. (B) Matrigel plugs were fixed, sectioned, and stained with H E. (C) Neovascularization of Matrigel plugs were quantified with Drabkin’s reagent using a spectrophometer. (D) Effect of BA145 and CQ on tumor growth in vivo. PC-3 M-luc2 cells implanted into NOD.SCID mice were treated with vehicle, BA145 (75 mg/kg), BA145 (75 mg/kg) plus CQ (50 mg/kg), positive control flutamide (25 mg/kg), and CQ (50 mg/kg) on alternate days for 28 days. Tumor volumes are reported as mean ± SE. (E) Representative pictures of excised tumors and their mass. (F) Western blot analysis of the indicated proteins in tumor tissues. β actin was used as loading control. Columns, mean; bars, SD; with ***p
    Figure Legend Snippet: Autophagy inhibition potentiates the anti-angiogenic effects of BA145 in vivo. (A) Effect of BA145 and CQ on VEGF induced angiogenesis. Six weeks old C57BL/6 J mice were injected with Matrigel containing BA145 (50 mg/kg), CQ (50 mg/kg), or both BA145 and CQ along with VEGF (150 ng) into the ventral area (6 mice per group). Matrigel plugs were monitored 10 days later. (B) Matrigel plugs were fixed, sectioned, and stained with H E. (C) Neovascularization of Matrigel plugs were quantified with Drabkin’s reagent using a spectrophometer. (D) Effect of BA145 and CQ on tumor growth in vivo. PC-3 M-luc2 cells implanted into NOD.SCID mice were treated with vehicle, BA145 (75 mg/kg), BA145 (75 mg/kg) plus CQ (50 mg/kg), positive control flutamide (25 mg/kg), and CQ (50 mg/kg) on alternate days for 28 days. Tumor volumes are reported as mean ± SE. (E) Representative pictures of excised tumors and their mass. (F) Western blot analysis of the indicated proteins in tumor tissues. β actin was used as loading control. Columns, mean; bars, SD; with ***p

    Techniques Used: Inhibition, In Vivo, Mouse Assay, Injection, Staining, Positive Control, Western Blot

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Microenvironment Changes (in pH) Affect VEGF Alternative Splicing
    Article Snippet: .. ELISA, Protein Extraction and Western Blotting Culture supernatants from RL95 in different conditions were collected and used to measure human VEGF by ELISA (Oncogene Research Products) under conditions described by the supplier. ..

    Protein Extraction:

    Article Title: Microenvironment Changes (in pH) Affect VEGF Alternative Splicing
    Article Snippet: .. ELISA, Protein Extraction and Western Blotting Culture supernatants from RL95 in different conditions were collected and used to measure human VEGF by ELISA (Oncogene Research Products) under conditions described by the supplier. ..

    other:

    Article Title: Lysosomal Pathways and Autophagy Distinctively Control Endothelial Cell Behavior to Affect Tumor Vasculature
    Article Snippet: Methyl cellulose (M6385), VEGF-A (SRP-3182), N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) (D5642), paraformaldehyde (P6148), DMSO (472301), Leupeptin (L-2884) and chloroquine diphosphate salt (C6628) were from Sigma-Aldrich (Bornem, Belgium).

    Expressing:

    Article Title: AAVrh.10-mediated Genetic Delivery of Bevacizumab to the Pleura to Provide Local Anti-VEGF to Suppress Growth of Metastatic Lung Tumors
    Article Snippet: .. Supernatants were concentrated by passage through Ultracel YM-10 centrifugal filters (Millipore, Billerica, MA) and evaluated for the expression of anti-human VEGF-A antibody by Western analysis under reducing conditions, using a peroxidase-conjugated sheep anti-mouse IgG secondary antibody (SIGMA, Saint Louis, MO) and enhanced chemiluminescence (ECL) reagent (GE Healthcare Life Sciences, Piscataway, NJ). .. The specificity of the AAVrh.10-expressed anti-VEGF antibody for human VEGF was determined by Western analysis with human VEGF-A121 and VEGF-A165 or with mouse VEGF-A120 and VEGF-A164 as the target antigens and infected cell supernatants as the primary antibody.

    Western Blot:

    Article Title: AAVrh.10-mediated Genetic Delivery of Bevacizumab to the Pleura to Provide Local Anti-VEGF to Suppress Growth of Metastatic Lung Tumors
    Article Snippet: .. Supernatants were concentrated by passage through Ultracel YM-10 centrifugal filters (Millipore, Billerica, MA) and evaluated for the expression of anti-human VEGF-A antibody by Western analysis under reducing conditions, using a peroxidase-conjugated sheep anti-mouse IgG secondary antibody (SIGMA, Saint Louis, MO) and enhanced chemiluminescence (ECL) reagent (GE Healthcare Life Sciences, Piscataway, NJ). .. The specificity of the AAVrh.10-expressed anti-VEGF antibody for human VEGF was determined by Western analysis with human VEGF-A121 and VEGF-A165 or with mouse VEGF-A120 and VEGF-A164 as the target antigens and infected cell supernatants as the primary antibody.

    Article Title: Differential Healing After Sirolimus, Paclitaxel and Bare Metal Stent Placement in Combination with PPARγ Agonists: Requirement for mTOR/Akt2 in PPARγ Activation
    Article Snippet: .. Paclitaxel and human VEGF were purchased from Sigma Antibodies used for Western blot were anti-phospho-Ser 2448 mTOR, anti-mTOR, anti-phospho-Thr 421/Ser424 p70 S6k Kinase, anti-p70 S6 Kinase, anti-phospho-Ser-65 4E-BP-1, anti-4E-BP-1, anti-phospho-Ser-473-Akt, anti-Akt all purchased from Cell Signaling (Beverly, MA). .. Anti β-actin was purchased from Abcam (Cambridge, MA).

    Article Title: Microenvironment Changes (in pH) Affect VEGF Alternative Splicing
    Article Snippet: .. ELISA, Protein Extraction and Western Blotting Culture supernatants from RL95 in different conditions were collected and used to measure human VEGF by ELISA (Oncogene Research Products) under conditions described by the supplier. ..

    Recombinant:

    Article Title: Protein Phosphotyrosine Phosphatase 1B (PTP1B) in Calpain-dependent Feedback Regulation of Vascular Endothelial Growth Factor Receptor (VEGFR2) in Endothelial Cells
    Article Snippet: .. Treatments were applied directly on the wound bed in PBS at total volume of 5 μl with or without recombinant human VEGF (200 ng) , ALLN (20 nmol), and PTP1B inhibitor (50 nmol, Calbiochem). .. Five micrograms of HA-tagged PTP1B and human calpain 1 and calpain 2 (2.5 μg each) plasmids were prepared with a lipid-based in vivo transfection kit and intracutaneously injected near the wound bed.

    Inhibition:

    Article Title: Adipokines and Vascular Endothelial Growth Factor in Normal Human Breast Tissue in Vivo – Correlations and Attenuation by Dietary Flaxseed
    Article Snippet: .. For the inhibition assays co-cultures were treated with anti-human leptin antibody (Genway, USA), anti-human VEGF antibody (Calbiochem, USA), or normal IgG from the same species (R & D system, USA) at 0.1 μg/ml and 1 μg/ml in presence or absence of 10−9 M estradiol. ..

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  • 99
    Millipore anti human vegf antibody
    Inhibition of <t>VEGF</t> decreased <t>leptin</t> secretion in co-culture of MCF7 and adipocytes. Co-culture of ER+ breast cancer cells, MCF7, and freshly isolated human adipocytes, were exposed to; a . Anti-VEGF antibody at 0.1 μg/ml and 1 μg/ml. Extracellular levels of leptin were analyzed after 48 h. *, p
    Anti Human Vegf Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human vegf antibody/product/Millipore
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    anti human vegf antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore vascular endothelial growth factor vegf
    Effect of high calorie environment towards oxidative stress and pro-inflammatory status in rat strains. After the completion of feeding experiment, blood was collected; plasma was separated and estimated the levels of pro-inflammatory cytokines. ( A ) Interleukin 6; IL-6. ( B ) Tumor necrosis factor alpha; TNFα. ( C ) Interleukin 1 beta; IL-1β. ( D ) Macrophage inflammatory protein 1 alpha; MIP-1α. ( E ) Vascular endothelial growth factor; <t>VEGF.</t> ( F ) <t>IFN</t> gamma inducible protein 10; IP-10. ( G ) Monocyte chemotactic protein 1; MCP1 and anti -inflammatory cytokines such as ( H ) Interleukin 4; IL-4 and ( I ) Interleukin 10; IL-10. ( J ) TBARS assay was performed on liver tissue lysate to study oxidative stress. Data was presented as mean ± SEM (n = 6 per group). Diets: control; HF, high fat; HS, high sucrose; HFS, high fat sucrose. TBARS, Thio Barbituricacid Reactive Substances. *P
    Vascular Endothelial Growth Factor Vegf, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular endothelial growth factor vegf/product/Millipore
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    vascular endothelial growth factor vegf - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Inhibition of VEGF decreased leptin secretion in co-culture of MCF7 and adipocytes. Co-culture of ER+ breast cancer cells, MCF7, and freshly isolated human adipocytes, were exposed to; a . Anti-VEGF antibody at 0.1 μg/ml and 1 μg/ml. Extracellular levels of leptin were analyzed after 48 h. *, p

    Journal: Journal of Mammary Gland Biology and Neoplasia

    Article Title: Adipokines and Vascular Endothelial Growth Factor in Normal Human Breast Tissue in Vivo – Correlations and Attenuation by Dietary Flaxseed

    doi: 10.1007/s10911-016-9352-9

    Figure Lengend Snippet: Inhibition of VEGF decreased leptin secretion in co-culture of MCF7 and adipocytes. Co-culture of ER+ breast cancer cells, MCF7, and freshly isolated human adipocytes, were exposed to; a . Anti-VEGF antibody at 0.1 μg/ml and 1 μg/ml. Extracellular levels of leptin were analyzed after 48 h. *, p

    Article Snippet: For the inhibition assays co-cultures were treated with anti-human leptin antibody (Genway, USA), anti-human VEGF antibody (Calbiochem, USA), or normal IgG from the same species (R & D system, USA) at 0.1 μg/ml and 1 μg/ml in presence or absence of 10−9 M estradiol.

    Techniques: Inhibition, Co-Culture Assay, Isolation

    Co-culture with adipocytes enhanced the effects of estradiol on leptin and VEGF secretion. A. MCF7 cells, freshly isolated human adipocytes or co-culture of MCF7 and adipocytes, were cultured with or without estradiol (10 −9 M) for 48 h. Extracellular levels of VEGF were measured in the conditioned medium with ELISA. ***, p

    Journal: Journal of Mammary Gland Biology and Neoplasia

    Article Title: Adipokines and Vascular Endothelial Growth Factor in Normal Human Breast Tissue in Vivo – Correlations and Attenuation by Dietary Flaxseed

    doi: 10.1007/s10911-016-9352-9

    Figure Lengend Snippet: Co-culture with adipocytes enhanced the effects of estradiol on leptin and VEGF secretion. A. MCF7 cells, freshly isolated human adipocytes or co-culture of MCF7 and adipocytes, were cultured with or without estradiol (10 −9 M) for 48 h. Extracellular levels of VEGF were measured in the conditioned medium with ELISA. ***, p

    Article Snippet: For the inhibition assays co-cultures were treated with anti-human leptin antibody (Genway, USA), anti-human VEGF antibody (Calbiochem, USA), or normal IgG from the same species (R & D system, USA) at 0.1 μg/ml and 1 μg/ml in presence or absence of 10−9 M estradiol.

    Techniques: Co-Culture Assay, Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

    VEGF correlated with leptin and the ratio of leptin : adiponectin in normal human breast tissue. Microdialysis was used for sampling of extracellular VEGF, leptin, and adiponectin in vivo in normal breast tissue (extracellular breast) and the abdominal subcutaneous (s.c.) fat (extracellular fat), in pre-and postmenopausal women. Filled circles represent women investigated in the luteal phase of the menstrual cycle and open circles women investigated in the follicular phase of the menstrual cycle. Open squares represent postmenopausal women. a . Extracellular VEGF levels correlated positively with extracellular leptin levels in normal breast tissue, r = 0.6; p

    Journal: Journal of Mammary Gland Biology and Neoplasia

    Article Title: Adipokines and Vascular Endothelial Growth Factor in Normal Human Breast Tissue in Vivo – Correlations and Attenuation by Dietary Flaxseed

    doi: 10.1007/s10911-016-9352-9

    Figure Lengend Snippet: VEGF correlated with leptin and the ratio of leptin : adiponectin in normal human breast tissue. Microdialysis was used for sampling of extracellular VEGF, leptin, and adiponectin in vivo in normal breast tissue (extracellular breast) and the abdominal subcutaneous (s.c.) fat (extracellular fat), in pre-and postmenopausal women. Filled circles represent women investigated in the luteal phase of the menstrual cycle and open circles women investigated in the follicular phase of the menstrual cycle. Open squares represent postmenopausal women. a . Extracellular VEGF levels correlated positively with extracellular leptin levels in normal breast tissue, r = 0.6; p

    Article Snippet: For the inhibition assays co-cultures were treated with anti-human leptin antibody (Genway, USA), anti-human VEGF antibody (Calbiochem, USA), or normal IgG from the same species (R & D system, USA) at 0.1 μg/ml and 1 μg/ml in presence or absence of 10−9 M estradiol.

    Techniques: Sampling, In Vivo

    a SR proteins involved in the control of the VEGF splicing pattern. Most SR proteins were up-regulated in acidic conditions both after 6 and 8 h of exposure. b Expression of the SR protein SRp20, in RL95, increased significantly ( p

    Journal: Cancer Microenvironment

    Article Title: Microenvironment Changes (in pH) Affect VEGF Alternative Splicing

    doi: 10.1007/s12307-008-0013-4

    Figure Lengend Snippet: a SR proteins involved in the control of the VEGF splicing pattern. Most SR proteins were up-regulated in acidic conditions both after 6 and 8 h of exposure. b Expression of the SR protein SRp20, in RL95, increased significantly ( p

    Article Snippet: ELISA, Protein Extraction and Western Blotting Culture supernatants from RL95 in different conditions were collected and used to measure human VEGF by ELISA (Oncogene Research Products) under conditions described by the supplier.

    Techniques: Expressing

    VEGF isoforms expression pattern by RL95 cells in response to changes in the microenvironment. By real time RT-PCR ( a ) and ELISA ( b), we can see an increase in VEGF production in acidic and hypoxic (mimicked by CoCl 2 ) conditions. A shift in the VEGF isoforms splicing pattern is more evident at pH 5.5 where VEGF121 expression is significantly different from all other isoforms ( p

    Journal: Cancer Microenvironment

    Article Title: Microenvironment Changes (in pH) Affect VEGF Alternative Splicing

    doi: 10.1007/s12307-008-0013-4

    Figure Lengend Snippet: VEGF isoforms expression pattern by RL95 cells in response to changes in the microenvironment. By real time RT-PCR ( a ) and ELISA ( b), we can see an increase in VEGF production in acidic and hypoxic (mimicked by CoCl 2 ) conditions. A shift in the VEGF isoforms splicing pattern is more evident at pH 5.5 where VEGF121 expression is significantly different from all other isoforms ( p

    Article Snippet: ELISA, Protein Extraction and Western Blotting Culture supernatants from RL95 in different conditions were collected and used to measure human VEGF by ELISA (Oncogene Research Products) under conditions described by the supplier.

    Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    Effect of high calorie environment towards oxidative stress and pro-inflammatory status in rat strains. After the completion of feeding experiment, blood was collected; plasma was separated and estimated the levels of pro-inflammatory cytokines. ( A ) Interleukin 6; IL-6. ( B ) Tumor necrosis factor alpha; TNFα. ( C ) Interleukin 1 beta; IL-1β. ( D ) Macrophage inflammatory protein 1 alpha; MIP-1α. ( E ) Vascular endothelial growth factor; VEGF. ( F ) IFN gamma inducible protein 10; IP-10. ( G ) Monocyte chemotactic protein 1; MCP1 and anti -inflammatory cytokines such as ( H ) Interleukin 4; IL-4 and ( I ) Interleukin 10; IL-10. ( J ) TBARS assay was performed on liver tissue lysate to study oxidative stress. Data was presented as mean ± SEM (n = 6 per group). Diets: control; HF, high fat; HS, high sucrose; HFS, high fat sucrose. TBARS, Thio Barbituricacid Reactive Substances. *P

    Journal: Scientific Reports

    Article Title: Differential response of rat strains to obesogenic diets underlines the importance of genetic makeup of an individual towards obesity

    doi: 10.1038/s41598-017-09149-6

    Figure Lengend Snippet: Effect of high calorie environment towards oxidative stress and pro-inflammatory status in rat strains. After the completion of feeding experiment, blood was collected; plasma was separated and estimated the levels of pro-inflammatory cytokines. ( A ) Interleukin 6; IL-6. ( B ) Tumor necrosis factor alpha; TNFα. ( C ) Interleukin 1 beta; IL-1β. ( D ) Macrophage inflammatory protein 1 alpha; MIP-1α. ( E ) Vascular endothelial growth factor; VEGF. ( F ) IFN gamma inducible protein 10; IP-10. ( G ) Monocyte chemotactic protein 1; MCP1 and anti -inflammatory cytokines such as ( H ) Interleukin 4; IL-4 and ( I ) Interleukin 10; IL-10. ( J ) TBARS assay was performed on liver tissue lysate to study oxidative stress. Data was presented as mean ± SEM (n = 6 per group). Diets: control; HF, high fat; HS, high sucrose; HFS, high fat sucrose. TBARS, Thio Barbituricacid Reactive Substances. *P

    Article Snippet: Rat Cytokine/Chemokine Profile The adipocytokines such as leptin, macrophage inflammatory protein-1 alpha (MIP-1α, CCL3), interleukin-4 (IL-4), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), macrophage chemo attractant protein-1 (MCP-1, CCL2), IFN-gamma-inducible protein 10 (IP- 10, CXCL10), vascular endothelial growth factor (VEGF), and tumor necrosis factor-alpha (TNF-α) were estimated both in circulation as well as in visceral adipose tissue by Milliplex Rat cytokine immunoassay kit (Millipore, USA) according to manufacturer’s instructions.

    Techniques: TBARS Assay

    In utero exposure to UFP induces systemic and intrauterine inflammation. Concentrations of pro-inflammatory cytokine: interleukin-β (IL-β), IL-6 and tumor necrosis factor-α (TNF-α). The anti-inflammatory cytokines IL-10 and IL-4. Chemokines: neutrophil chemoattractant (KC), macrophage inflammatory protein 2 (MIP-2) and monocyte chemoattractant protein 1 (MCP-1). Vascular endothelial growth factor (VEGF) ware measured with multiplex magnetic beads in a ) dam serum collected, b ) dam lung, c ) placenta and d ) fetus total protein. n = 4/group. Data are expressed as mean ± SEM. * p

    Journal: Particle and Fibre Toxicology

    Article Title: In utero exposure to ultrafine particles promotes placental stress-induced programming of renin-angiotensin system-related elements in the offspring results in altered blood pressure in adult mice

    doi: 10.1186/s12989-019-0289-1

    Figure Lengend Snippet: In utero exposure to UFP induces systemic and intrauterine inflammation. Concentrations of pro-inflammatory cytokine: interleukin-β (IL-β), IL-6 and tumor necrosis factor-α (TNF-α). The anti-inflammatory cytokines IL-10 and IL-4. Chemokines: neutrophil chemoattractant (KC), macrophage inflammatory protein 2 (MIP-2) and monocyte chemoattractant protein 1 (MCP-1). Vascular endothelial growth factor (VEGF) ware measured with multiplex magnetic beads in a ) dam serum collected, b ) dam lung, c ) placenta and d ) fetus total protein. n = 4/group. Data are expressed as mean ± SEM. * p

    Article Snippet: Analysis of cytokines and chemokines The pro-inflammatory cytokines interleukin-β (IL-β), IL-6 and tumor necrosis factor-α (TNF-α), the anti-inflammatory cytokines IL-10 and IL-4, chemokines including neutrophil chemoattractant (KC), macrophage inflammatory protein 2 (MIP-2) and monocyte chemoattractant protein 1 (MCP-1), and vascular endothelial growth factor (VEGF) were measured in the dam sera and the total protein of the dam lungs, placentas and fetuses using an MCYTOMAG-70 K Milliplex MAP Mouse Cytokine/Chemokine magnetic bead panel (EMD Millipore, Billerica, MA, USA) following the manufacturer’s instructions.

    Techniques: In Utero, Multiplex Assay, Magnetic Beads