vegf c elisa kit  (Boster Bio)


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    Boster Bio vegf c elisa kit
    Vegf C Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf c elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf c elisa kit - by Bioz Stars, 2022-10
    94/100 stars

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    Boster Bio human vegf c elisa kit
    USP12-induced angiogenesis in breast cancer. A The relative expression of VEGF-A and <t>VEGF-C</t> in the supernatant of MDA-MB-231 cells overexpressing USP12 was detected by <t>ELISA.</t> B The relative expression of VEGF-A and VEGF-C in the supernatant of MDA-MB-231 cells with USP12 knockdown was detected by ELISA. C , D Migration of HUVECs treated with supernatant from MDA-MB-231 cells with USP12 overexpression ( C ) and USP12 knockdown ( D ). Representative images are shown in the left panel. The quantitative results are shown in the right panel. E , F . Tube formation assay of HUVECs treated with supernatant from MDA-MB-231 cells with USP12 overexpression ( E ) and USP12 knockdown ( F ). Representative images are shown in the left panel. The quantitative results are shown in the right panel. The experiments were repeated three times. ** p
    Human Vegf C Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human vegf c elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human vegf c elisa kit - by Bioz Stars, 2022-10
    94/100 stars
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    USP12-induced angiogenesis in breast cancer. A The relative expression of VEGF-A and VEGF-C in the supernatant of MDA-MB-231 cells overexpressing USP12 was detected by ELISA. B The relative expression of VEGF-A and VEGF-C in the supernatant of MDA-MB-231 cells with USP12 knockdown was detected by ELISA. C , D Migration of HUVECs treated with supernatant from MDA-MB-231 cells with USP12 overexpression ( C ) and USP12 knockdown ( D ). Representative images are shown in the left panel. The quantitative results are shown in the right panel. E , F . Tube formation assay of HUVECs treated with supernatant from MDA-MB-231 cells with USP12 overexpression ( E ) and USP12 knockdown ( F ). Representative images are shown in the left panel. The quantitative results are shown in the right panel. The experiments were repeated three times. ** p

    Journal: Cell Death & Disease

    Article Title: USP12 promotes breast cancer angiogenesis by maintaining midkine stability

    doi: 10.1038/s41419-021-04102-y

    Figure Lengend Snippet: USP12-induced angiogenesis in breast cancer. A The relative expression of VEGF-A and VEGF-C in the supernatant of MDA-MB-231 cells overexpressing USP12 was detected by ELISA. B The relative expression of VEGF-A and VEGF-C in the supernatant of MDA-MB-231 cells with USP12 knockdown was detected by ELISA. C , D Migration of HUVECs treated with supernatant from MDA-MB-231 cells with USP12 overexpression ( C ) and USP12 knockdown ( D ). Representative images are shown in the left panel. The quantitative results are shown in the right panel. E , F . Tube formation assay of HUVECs treated with supernatant from MDA-MB-231 cells with USP12 overexpression ( E ) and USP12 knockdown ( F ). Representative images are shown in the left panel. The quantitative results are shown in the right panel. The experiments were repeated three times. ** p

    Article Snippet: Matrigel matrix (356234, Corning), the human VEGF-A ELISA kit (EKO588-96, Boster) and the human VEGF-C ELISA KIT (EKO539-96, Boster) were also used.

    Techniques: Expressing, Multiple Displacement Amplification, Enzyme-linked Immunosorbent Assay, Migration, Over Expression, Tube Formation Assay

    Vascular endothelial growth factor C (VEGF-C) is involved in nuclear factors of activated T cells 5 (NFAT5)-driven progression of metastatic breast cancers. (A) In vitro tube formation of human umbilical vein endothelial cells (HUVECs) after co-culture for indicated time with MDA-MB-231 cells either mocked transfected or transfected with indicated siRNAs or miRNA mimic (×100). (B - D) ELISA using the serum samples for comparison of VEGF-C levels in healthy volunteers, patients with benign lesions, and patients with breast cancers (B) , in patients with breast cancers in various stages (C) , and in patients with non-metastatic and metastatic breast cancers (D) .

    Journal: Breast Cancer Research : BCR

    Article Title: Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis

    doi: 10.1186/s13058-014-0454-2

    Figure Lengend Snippet: Vascular endothelial growth factor C (VEGF-C) is involved in nuclear factors of activated T cells 5 (NFAT5)-driven progression of metastatic breast cancers. (A) In vitro tube formation of human umbilical vein endothelial cells (HUVECs) after co-culture for indicated time with MDA-MB-231 cells either mocked transfected or transfected with indicated siRNAs or miRNA mimic (×100). (B - D) ELISA using the serum samples for comparison of VEGF-C levels in healthy volunteers, patients with benign lesions, and patients with breast cancers (B) , in patients with breast cancers in various stages (C) , and in patients with non-metastatic and metastatic breast cancers (D) .

    Article Snippet: ELISA assay VEGF-C protein in culture medium or patient peripheral blood samples was measured using a human VEGF-C ELISA kit (Boster, Wuhan, China).

    Techniques: In Vitro, Co-Culture Assay, Multiple Displacement Amplification, Transfection, Enzyme-linked Immunosorbent Assay

    S100A4 and vascular endothelial growth factor C (VEGF-C) are transcriptionally activated by nuclear factors of activated T cells 5 (NFAT5). (A) qRT-PCR assays of the indicated transcripts in breast cancer cell lines. Relative levels compared with MCF-10A cells were plotted. (B) Western blotting analysis (left) and ELISA quantification (right) of cellular S100A4 and secreted VEGF-C, respectively, in the indicated cell lines. (C) RT-PCR (left), western blotting (S100A4) and ELISA (secreted VEGF-C) assays for indicated gene expression. (D , E) RT-PCR (D) , western blotting and ELISA (E) assays for gene expression and after transfection of MDA-MB-231 cells with indicated siRNAs. (F) Chromatin immunoprecipitation (ChIP) assay using the lysates of indicated breast cancer cells. All data are representative photographs or represent the mean ± SD of three replicates. * P

    Journal: Breast Cancer Research : BCR

    Article Title: Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis

    doi: 10.1186/s13058-014-0454-2

    Figure Lengend Snippet: S100A4 and vascular endothelial growth factor C (VEGF-C) are transcriptionally activated by nuclear factors of activated T cells 5 (NFAT5). (A) qRT-PCR assays of the indicated transcripts in breast cancer cell lines. Relative levels compared with MCF-10A cells were plotted. (B) Western blotting analysis (left) and ELISA quantification (right) of cellular S100A4 and secreted VEGF-C, respectively, in the indicated cell lines. (C) RT-PCR (left), western blotting (S100A4) and ELISA (secreted VEGF-C) assays for indicated gene expression. (D , E) RT-PCR (D) , western blotting and ELISA (E) assays for gene expression and after transfection of MDA-MB-231 cells with indicated siRNAs. (F) Chromatin immunoprecipitation (ChIP) assay using the lysates of indicated breast cancer cells. All data are representative photographs or represent the mean ± SD of three replicates. * P

    Article Snippet: ELISA assay VEGF-C protein in culture medium or patient peripheral blood samples was measured using a human VEGF-C ELISA kit (Boster, Wuhan, China).

    Techniques: Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Transfection, Multiple Displacement Amplification, Chromatin Immunoprecipitation

    LPS treatment enhances VEGF-C expression in colorectal cancer cells ( A – C ) The mRNA of TLR4, VEGF-C and VEGFR3 in the mock, LPS-stimulated (1 μg/ml) sw480 and Hct116 colorectal cells by real-time PCR. ( D ) The protein expression of VEGF-C from the mock, LPS-stimulated sw480 and Hct116 colorectal cells by ELISA. ( E ) VEGF-C mRNA expression in the mock, LPS-stimulated (1 μg/ml) sw480 and Hct116 cells by agarose gel electrophoresis. ( F ) The protein expression of VEGF-C from the mock, LPS-stimulated sw480 and Hct116 colorectal cells by western blot. Error bars represent mean ± SEM, representative of three experiments, * p

    Journal: Oncotarget

    Article Title: Lipopolysaccharide increases the release of VEGF-C that enhances cell motility and promotes lymphangiogenesis and lymphatic metastasis through the TLR4- NF-κB/JNK pathways in colorectal cancer

    doi: 10.18632/oncotarget.12449

    Figure Lengend Snippet: LPS treatment enhances VEGF-C expression in colorectal cancer cells ( A – C ) The mRNA of TLR4, VEGF-C and VEGFR3 in the mock, LPS-stimulated (1 μg/ml) sw480 and Hct116 colorectal cells by real-time PCR. ( D ) The protein expression of VEGF-C from the mock, LPS-stimulated sw480 and Hct116 colorectal cells by ELISA. ( E ) VEGF-C mRNA expression in the mock, LPS-stimulated (1 μg/ml) sw480 and Hct116 cells by agarose gel electrophoresis. ( F ) The protein expression of VEGF-C from the mock, LPS-stimulated sw480 and Hct116 colorectal cells by western blot. Error bars represent mean ± SEM, representative of three experiments, * p

    Article Snippet: Human VEGF-C ELISA Kit was purchased from Boster Biological Technology (Wuhan, China).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Agarose Gel Electrophoresis, Western Blot