Human Tnfsf11 Rankl Elisa Kit Picokine, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Bioengineering the ameloblastoma tumour to study its effect on bone nodule formation"
Article Title: Bioengineering the ameloblastoma tumour to study its effect on bone nodule formation
Journal: Scientific Reports
Figure Legend Snippet: Characterisation of ameloblastoma cells in 3D culture. Spheroid formation of ( a) AM-1 cells and ( b) AM-3 cells in 3D tumouroids at day 7, red = Phalloidin, blue = DAPI, 20 × Magnification Scale Bar = 50 μm. ( c) Histology H E staining of plexiform patient samples, ( d) 3D AM-1 tumouroids, e follicular patient samples, and (f) 3D AM-3 tumouroids. Similar anastomosing cords and branches were highlighted ( c, d) 10 × Magnification Scale Bar = 100 μm. The formation of the odontogenic islands was highlighted ( e,f) . ( g) CellTiter-Glo 3D Viability-Assay of AM-1, AM-3 and MG-63 cells in 3D tumouroids. Distance of invasion (μm) AM-1, AM-3 and MG-63 cells from the tumour mass to the surrounding stroma within the 3D tumouroids. ( h) Human MMP-2 ELISA. Expression of MMP2. ( i) Human MMP-9 ELISA. Expression of MMP9. ( j) Expression of TNFRSF11A (RANK). Human RANKL ELISA. One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.
Techniques Used: Staining, Viability Assay, Enzyme-linked Immunosorbent Assay, Expressing
Figure Legend Snippet: Introduction of ameloblastoma tumour mass to the 3D bone stroma prior to day 9 completely inhibits bone nodule formation. (a) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 6 inhibits bone nodule formation. Images taken at day 6, 9 and 21, 4 × Magnification, scale bar = 100 μm. ALPL expression. TNFSF11 (RANKL) Expression. ( b) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 9 restricts bone formation by limiting bone nodule number and bone nodule surface area. (c) RT2 Profiler PCR Array was conducted to screen osteogenesis gene of osteoblasts in the 3D bone stroma model and in AM-3 tumour mass introduced 3D bone stroma model at day 8. The AM-3 tumour mass was introduced at day 6 of 3D bone stroma model. Volcano plot shows under-expressed, unchanged and over-expressed genes. The table represents > 3.5-fold under-expressed gene. Horizontal line p-value threshold (0.05). One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.
Techniques Used: Expressing, Polymerase Chain Reaction