human tgf β2 picokine elisa kit  (Boster Bio)


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    Boster Bio human tgf β2 picokine elisa kit
    hUC-MSCs and hPSC-EMSCs exhibit different gene expression profile by RNA-seq analysis. A) Global gene expression profile analysis (hPSC-EMSCs (n = 3) Vs. hUC-MSCs (n = 3)) by RNA-seq indicated that hUC-MSCs and hPSC-EMSCs had different gene expression profiles from each other; B) Pearson's correlation analysis of hUC-MSCs and hPSC-EMSCs indicated that EMSCs derived from hPSCs was closely related to the EMSCs derived from hESCs and were distinct from hUC-MSCs. The correlation plot was generated using an online platform ( http://www.bioinformatics.com.cn/ ); C) Comparison between hUC-MSCs and hPSC-EMSCs by MA plot indicated that 1212 genes were differentially upregulated and 716 genes were differentially downregulated in hPSC-EMSCs compared to hUC-MSCs (DEGs, Log 2 Fold Change ≤ -1 or ≥ 1, P adj ≤ 0.05 or > 0.05); D) Heatmap analysis showing the differentially expressed genes associated with ECM, immunomodulation, neuroactivation and axon guidance between hUC-MSCs and hPSC-EMSCs; E) Gene ontology analysis of secretory factors (GO: 0032940) indicated that genes associated with ERK1 and ERK2 cascade were differentially expressed in hUC-MSCs and hPSC-EMSCs; F) <t>ELISA</t> analysis of β-NGF, <t>TGF-β</t> 2 , PDGF-AA and CCL5 in the CM derived from hPSC-EMSCs and hUC-MSCs. Three different EMSC-CM (from H7, H9 and iPSCs) and three UC-CM (from 009, 011 and 013) were used for the analysis. *** and **** represent p < 0.001 and 0.0001 respectively by Student T-test.
    Human Tgf β2 Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tgf β2 picokine elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human tgf β2 picokine elisa kit - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Human pluripotent stem cell-derived ectomesenchymal stromal cells promote more robust functional recovery than umbilical cord-derived mesenchymal stromal cells after hypoxic-ischaemic brain damage"

    Article Title: Human pluripotent stem cell-derived ectomesenchymal stromal cells promote more robust functional recovery than umbilical cord-derived mesenchymal stromal cells after hypoxic-ischaemic brain damage

    Journal: Theranostics

    doi: 10.7150/thno.57234

    hUC-MSCs and hPSC-EMSCs exhibit different gene expression profile by RNA-seq analysis. A) Global gene expression profile analysis (hPSC-EMSCs (n = 3) Vs. hUC-MSCs (n = 3)) by RNA-seq indicated that hUC-MSCs and hPSC-EMSCs had different gene expression profiles from each other; B) Pearson's correlation analysis of hUC-MSCs and hPSC-EMSCs indicated that EMSCs derived from hPSCs was closely related to the EMSCs derived from hESCs and were distinct from hUC-MSCs. The correlation plot was generated using an online platform ( http://www.bioinformatics.com.cn/ ); C) Comparison between hUC-MSCs and hPSC-EMSCs by MA plot indicated that 1212 genes were differentially upregulated and 716 genes were differentially downregulated in hPSC-EMSCs compared to hUC-MSCs (DEGs, Log 2 Fold Change ≤ -1 or ≥ 1, P adj ≤ 0.05 or > 0.05); D) Heatmap analysis showing the differentially expressed genes associated with ECM, immunomodulation, neuroactivation and axon guidance between hUC-MSCs and hPSC-EMSCs; E) Gene ontology analysis of secretory factors (GO: 0032940) indicated that genes associated with ERK1 and ERK2 cascade were differentially expressed in hUC-MSCs and hPSC-EMSCs; F) ELISA analysis of β-NGF, TGF-β 2 , PDGF-AA and CCL5 in the CM derived from hPSC-EMSCs and hUC-MSCs. Three different EMSC-CM (from H7, H9 and iPSCs) and three UC-CM (from 009, 011 and 013) were used for the analysis. *** and **** represent p < 0.001 and 0.0001 respectively by Student T-test.
    Figure Legend Snippet: hUC-MSCs and hPSC-EMSCs exhibit different gene expression profile by RNA-seq analysis. A) Global gene expression profile analysis (hPSC-EMSCs (n = 3) Vs. hUC-MSCs (n = 3)) by RNA-seq indicated that hUC-MSCs and hPSC-EMSCs had different gene expression profiles from each other; B) Pearson's correlation analysis of hUC-MSCs and hPSC-EMSCs indicated that EMSCs derived from hPSCs was closely related to the EMSCs derived from hESCs and were distinct from hUC-MSCs. The correlation plot was generated using an online platform ( http://www.bioinformatics.com.cn/ ); C) Comparison between hUC-MSCs and hPSC-EMSCs by MA plot indicated that 1212 genes were differentially upregulated and 716 genes were differentially downregulated in hPSC-EMSCs compared to hUC-MSCs (DEGs, Log 2 Fold Change ≤ -1 or ≥ 1, P adj ≤ 0.05 or > 0.05); D) Heatmap analysis showing the differentially expressed genes associated with ECM, immunomodulation, neuroactivation and axon guidance between hUC-MSCs and hPSC-EMSCs; E) Gene ontology analysis of secretory factors (GO: 0032940) indicated that genes associated with ERK1 and ERK2 cascade were differentially expressed in hUC-MSCs and hPSC-EMSCs; F) ELISA analysis of β-NGF, TGF-β 2 , PDGF-AA and CCL5 in the CM derived from hPSC-EMSCs and hUC-MSCs. Three different EMSC-CM (from H7, H9 and iPSCs) and three UC-CM (from 009, 011 and 013) were used for the analysis. *** and **** represent p < 0.001 and 0.0001 respectively by Student T-test.

    Techniques Used: Expressing, RNA Sequencing Assay, Derivative Assay, Generated, Enzyme-linked Immunosorbent Assay

    human tgf β2 picokine elisa kit  (Boster Bio)


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  • 94

    Structured Review

    Boster Bio human tgf β2 picokine elisa kit
    hUC-MSCs and hPSC-EMSCs exhibit different gene expression profile by RNA-seq analysis. A) Global gene expression profile analysis (hPSC-EMSCs (n = 3) Vs. hUC-MSCs (n = 3)) by RNA-seq indicated that hUC-MSCs and hPSC-EMSCs had different gene expression profiles from each other; B) Pearson's correlation analysis of hUC-MSCs and hPSC-EMSCs indicated that EMSCs derived from hPSCs was closely related to the EMSCs derived from hESCs and were distinct from hUC-MSCs. The correlation plot was generated using an online platform ( http://www.bioinformatics.com.cn/ ); C) Comparison between hUC-MSCs and hPSC-EMSCs by MA plot indicated that 1212 genes were differentially upregulated and 716 genes were differentially downregulated in hPSC-EMSCs compared to hUC-MSCs (DEGs, Log 2 Fold Change ≤ -1 or ≥ 1, P adj ≤ 0.05 or > 0.05); D) Heatmap analysis showing the differentially expressed genes associated with ECM, immunomodulation, neuroactivation and axon guidance between hUC-MSCs and hPSC-EMSCs; E) Gene ontology analysis of secretory factors (GO: 0032940) indicated that genes associated with ERK1 and ERK2 cascade were differentially expressed in hUC-MSCs and hPSC-EMSCs; F) <t>ELISA</t> analysis of β-NGF, <t>TGF-β</t> 2 , PDGF-AA and CCL5 in the CM derived from hPSC-EMSCs and hUC-MSCs. Three different EMSC-CM (from H7, H9 and iPSCs) and three UC-CM (from 009, 011 and 013) were used for the analysis. *** and **** represent p < 0.001 and 0.0001 respectively by Student T-test.
    Human Tgf β2 Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tgf β2 picokine elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human tgf β2 picokine elisa kit - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Human pluripotent stem cell-derived ectomesenchymal stromal cells promote more robust functional recovery than umbilical cord-derived mesenchymal stromal cells after hypoxic-ischaemic brain damage"

    Article Title: Human pluripotent stem cell-derived ectomesenchymal stromal cells promote more robust functional recovery than umbilical cord-derived mesenchymal stromal cells after hypoxic-ischaemic brain damage

    Journal: Theranostics

    doi: 10.7150/thno.57234

    hUC-MSCs and hPSC-EMSCs exhibit different gene expression profile by RNA-seq analysis. A) Global gene expression profile analysis (hPSC-EMSCs (n = 3) Vs. hUC-MSCs (n = 3)) by RNA-seq indicated that hUC-MSCs and hPSC-EMSCs had different gene expression profiles from each other; B) Pearson's correlation analysis of hUC-MSCs and hPSC-EMSCs indicated that EMSCs derived from hPSCs was closely related to the EMSCs derived from hESCs and were distinct from hUC-MSCs. The correlation plot was generated using an online platform ( http://www.bioinformatics.com.cn/ ); C) Comparison between hUC-MSCs and hPSC-EMSCs by MA plot indicated that 1212 genes were differentially upregulated and 716 genes were differentially downregulated in hPSC-EMSCs compared to hUC-MSCs (DEGs, Log 2 Fold Change ≤ -1 or ≥ 1, P adj ≤ 0.05 or > 0.05); D) Heatmap analysis showing the differentially expressed genes associated with ECM, immunomodulation, neuroactivation and axon guidance between hUC-MSCs and hPSC-EMSCs; E) Gene ontology analysis of secretory factors (GO: 0032940) indicated that genes associated with ERK1 and ERK2 cascade were differentially expressed in hUC-MSCs and hPSC-EMSCs; F) ELISA analysis of β-NGF, TGF-β 2 , PDGF-AA and CCL5 in the CM derived from hPSC-EMSCs and hUC-MSCs. Three different EMSC-CM (from H7, H9 and iPSCs) and three UC-CM (from 009, 011 and 013) were used for the analysis. *** and **** represent p < 0.001 and 0.0001 respectively by Student T-test.
    Figure Legend Snippet: hUC-MSCs and hPSC-EMSCs exhibit different gene expression profile by RNA-seq analysis. A) Global gene expression profile analysis (hPSC-EMSCs (n = 3) Vs. hUC-MSCs (n = 3)) by RNA-seq indicated that hUC-MSCs and hPSC-EMSCs had different gene expression profiles from each other; B) Pearson's correlation analysis of hUC-MSCs and hPSC-EMSCs indicated that EMSCs derived from hPSCs was closely related to the EMSCs derived from hESCs and were distinct from hUC-MSCs. The correlation plot was generated using an online platform ( http://www.bioinformatics.com.cn/ ); C) Comparison between hUC-MSCs and hPSC-EMSCs by MA plot indicated that 1212 genes were differentially upregulated and 716 genes were differentially downregulated in hPSC-EMSCs compared to hUC-MSCs (DEGs, Log 2 Fold Change ≤ -1 or ≥ 1, P adj ≤ 0.05 or > 0.05); D) Heatmap analysis showing the differentially expressed genes associated with ECM, immunomodulation, neuroactivation and axon guidance between hUC-MSCs and hPSC-EMSCs; E) Gene ontology analysis of secretory factors (GO: 0032940) indicated that genes associated with ERK1 and ERK2 cascade were differentially expressed in hUC-MSCs and hPSC-EMSCs; F) ELISA analysis of β-NGF, TGF-β 2 , PDGF-AA and CCL5 in the CM derived from hPSC-EMSCs and hUC-MSCs. Three different EMSC-CM (from H7, H9 and iPSCs) and three UC-CM (from 009, 011 and 013) were used for the analysis. *** and **** represent p < 0.001 and 0.0001 respectively by Student T-test.

    Techniques Used: Expressing, RNA Sequencing Assay, Derivative Assay, Generated, Enzyme-linked Immunosorbent Assay

    human tgf β2 elisa assay  (Boster Bio)


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    Boster Bio human tgf β2 elisa assay
    Human Tgf β2 Elisa Assay, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tgf β2 elisa assay/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human tgf β2 elisa assay - by Bioz Stars, 2023-01
    94/100 stars

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    il 2 kit  (Boster Bio)


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    Boster Bio il 2 kit
    Rg1’s Effect upon Inflammatory Cytokine Secretion by BMSCs in Aged Rats. The BMSCs were collected on day 5 of passage 3. (A) Interleukin (IL)-6, <t>(B)</t> <t>IL-2,</t> and (C) tumor necrosis factor (TNF)-α. a: comparison with the control group ( p < 0.05); b: comparison with the D-gal-administration group ( p < 0.05). Each error bar represents the standard deviation (SD).
    Il 2 Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 2 kit/product/Boster Bio
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 2 kit - by Bioz Stars, 2023-01
    85/100 stars

    Images

    1) Product Images from "The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model"

    Article Title: The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model

    Journal: BMC Complementary and Alternative Medicine

    doi: 10.1186/s12906-015-0642-3

    Rg1’s Effect upon Inflammatory Cytokine Secretion by BMSCs in Aged Rats. The BMSCs were collected on day 5 of passage 3. (A) Interleukin (IL)-6, (B) IL-2, and (C) tumor necrosis factor (TNF)-α. a: comparison with the control group ( p < 0.05); b: comparison with the D-gal-administration group ( p < 0.05). Each error bar represents the standard deviation (SD).
    Figure Legend Snippet: Rg1’s Effect upon Inflammatory Cytokine Secretion by BMSCs in Aged Rats. The BMSCs were collected on day 5 of passage 3. (A) Interleukin (IL)-6, (B) IL-2, and (C) tumor necrosis factor (TNF)-α. a: comparison with the control group ( p < 0.05); b: comparison with the D-gal-administration group ( p < 0.05). Each error bar represents the standard deviation (SD).

    Techniques Used: Standard Deviation

    tgf b1  (Boster Bio)


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    Boster Bio tgf b1
    Tgf B1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tgf b1/product/Boster Bio
    Average 91 stars, based on 1 article reviews
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    tgf b1 - by Bioz Stars, 2023-01
    91/100 stars

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    human tgfbeta  (Boster Bio)


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    Boster Bio human tgfbeta
    Human Tgfbeta, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tgfbeta/product/Boster Bio
    Average 94 stars, based on 1 article reviews
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    human tgfbeta - by Bioz Stars, 2023-01
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    Boster Bio human tgf β2 picokine elisa kit
    hUC-MSCs and hPSC-EMSCs exhibit different gene expression profile by RNA-seq analysis. A) Global gene expression profile analysis (hPSC-EMSCs (n = 3) Vs. hUC-MSCs (n = 3)) by RNA-seq indicated that hUC-MSCs and hPSC-EMSCs had different gene expression profiles from each other; B) Pearson's correlation analysis of hUC-MSCs and hPSC-EMSCs indicated that EMSCs derived from hPSCs was closely related to the EMSCs derived from hESCs and were distinct from hUC-MSCs. The correlation plot was generated using an online platform ( http://www.bioinformatics.com.cn/ ); C) Comparison between hUC-MSCs and hPSC-EMSCs by MA plot indicated that 1212 genes were differentially upregulated and 716 genes were differentially downregulated in hPSC-EMSCs compared to hUC-MSCs (DEGs, Log 2 Fold Change ≤ -1 or ≥ 1, P adj ≤ 0.05 or > 0.05); D) Heatmap analysis showing the differentially expressed genes associated with ECM, immunomodulation, neuroactivation and axon guidance between hUC-MSCs and hPSC-EMSCs; E) Gene ontology analysis of secretory factors (GO: 0032940) indicated that genes associated with ERK1 and ERK2 cascade were differentially expressed in hUC-MSCs and hPSC-EMSCs; F) <t>ELISA</t> analysis of β-NGF, <t>TGF-β</t> 2 , PDGF-AA and CCL5 in the CM derived from hPSC-EMSCs and hUC-MSCs. Three different EMSC-CM (from H7, H9 and iPSCs) and three UC-CM (from 009, 011 and 013) were used for the analysis. *** and **** represent p < 0.001 and 0.0001 respectively by Student T-test.
    Human Tgf β2 Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tgf β2 picokine elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human tgf β2 picokine elisa kit - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    94
    Boster Bio human tgf β2 elisa assay
    hUC-MSCs and hPSC-EMSCs exhibit different gene expression profile by RNA-seq analysis. A) Global gene expression profile analysis (hPSC-EMSCs (n = 3) Vs. hUC-MSCs (n = 3)) by RNA-seq indicated that hUC-MSCs and hPSC-EMSCs had different gene expression profiles from each other; B) Pearson's correlation analysis of hUC-MSCs and hPSC-EMSCs indicated that EMSCs derived from hPSCs was closely related to the EMSCs derived from hESCs and were distinct from hUC-MSCs. The correlation plot was generated using an online platform ( http://www.bioinformatics.com.cn/ ); C) Comparison between hUC-MSCs and hPSC-EMSCs by MA plot indicated that 1212 genes were differentially upregulated and 716 genes were differentially downregulated in hPSC-EMSCs compared to hUC-MSCs (DEGs, Log 2 Fold Change ≤ -1 or ≥ 1, P adj ≤ 0.05 or > 0.05); D) Heatmap analysis showing the differentially expressed genes associated with ECM, immunomodulation, neuroactivation and axon guidance between hUC-MSCs and hPSC-EMSCs; E) Gene ontology analysis of secretory factors (GO: 0032940) indicated that genes associated with ERK1 and ERK2 cascade were differentially expressed in hUC-MSCs and hPSC-EMSCs; F) <t>ELISA</t> analysis of β-NGF, <t>TGF-β</t> 2 , PDGF-AA and CCL5 in the CM derived from hPSC-EMSCs and hUC-MSCs. Three different EMSC-CM (from H7, H9 and iPSCs) and three UC-CM (from 009, 011 and 013) were used for the analysis. *** and **** represent p < 0.001 and 0.0001 respectively by Student T-test.
    Human Tgf β2 Elisa Assay, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tgf β2 elisa assay/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human tgf β2 elisa assay - by Bioz Stars, 2023-01
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    Boster Bio il 2 kit
    Rg1’s Effect upon Inflammatory Cytokine Secretion by BMSCs in Aged Rats. The BMSCs were collected on day 5 of passage 3. (A) Interleukin (IL)-6, <t>(B)</t> <t>IL-2,</t> and (C) tumor necrosis factor (TNF)-α. a: comparison with the control group ( p < 0.05); b: comparison with the D-gal-administration group ( p < 0.05). Each error bar represents the standard deviation (SD).
    Il 2 Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 2 kit/product/Boster Bio
    Average 85 stars, based on 1 article reviews
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    il 2 kit - by Bioz Stars, 2023-01
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    Boster Bio tgf b1
    Rg1’s Effect upon Inflammatory Cytokine Secretion by BMSCs in Aged Rats. The BMSCs were collected on day 5 of passage 3. (A) Interleukin (IL)-6, <t>(B)</t> <t>IL-2,</t> and (C) tumor necrosis factor (TNF)-α. a: comparison with the control group ( p < 0.05); b: comparison with the D-gal-administration group ( p < 0.05). Each error bar represents the standard deviation (SD).
    Tgf B1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tgf b1/product/Boster Bio
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    Boster Bio human tgfbeta
    Rg1’s Effect upon Inflammatory Cytokine Secretion by BMSCs in Aged Rats. The BMSCs were collected on day 5 of passage 3. (A) Interleukin (IL)-6, <t>(B)</t> <t>IL-2,</t> and (C) tumor necrosis factor (TNF)-α. a: comparison with the control group ( p < 0.05); b: comparison with the D-gal-administration group ( p < 0.05). Each error bar represents the standard deviation (SD).
    Human Tgfbeta, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tgfbeta/product/Boster Bio
    Average 94 stars, based on 1 article reviews
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    human tgfbeta - by Bioz Stars, 2023-01
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    Image Search Results


    hUC-MSCs and hPSC-EMSCs exhibit different gene expression profile by RNA-seq analysis. A) Global gene expression profile analysis (hPSC-EMSCs (n = 3) Vs. hUC-MSCs (n = 3)) by RNA-seq indicated that hUC-MSCs and hPSC-EMSCs had different gene expression profiles from each other; B) Pearson's correlation analysis of hUC-MSCs and hPSC-EMSCs indicated that EMSCs derived from hPSCs was closely related to the EMSCs derived from hESCs and were distinct from hUC-MSCs. The correlation plot was generated using an online platform ( http://www.bioinformatics.com.cn/ ); C) Comparison between hUC-MSCs and hPSC-EMSCs by MA plot indicated that 1212 genes were differentially upregulated and 716 genes were differentially downregulated in hPSC-EMSCs compared to hUC-MSCs (DEGs, Log 2 Fold Change ≤ -1 or ≥ 1, P adj ≤ 0.05 or > 0.05); D) Heatmap analysis showing the differentially expressed genes associated with ECM, immunomodulation, neuroactivation and axon guidance between hUC-MSCs and hPSC-EMSCs; E) Gene ontology analysis of secretory factors (GO: 0032940) indicated that genes associated with ERK1 and ERK2 cascade were differentially expressed in hUC-MSCs and hPSC-EMSCs; F) ELISA analysis of β-NGF, TGF-β 2 , PDGF-AA and CCL5 in the CM derived from hPSC-EMSCs and hUC-MSCs. Three different EMSC-CM (from H7, H9 and iPSCs) and three UC-CM (from 009, 011 and 013) were used for the analysis. *** and **** represent p < 0.001 and 0.0001 respectively by Student T-test.

    Journal: Theranostics

    Article Title: Human pluripotent stem cell-derived ectomesenchymal stromal cells promote more robust functional recovery than umbilical cord-derived mesenchymal stromal cells after hypoxic-ischaemic brain damage

    doi: 10.7150/thno.57234

    Figure Lengend Snippet: hUC-MSCs and hPSC-EMSCs exhibit different gene expression profile by RNA-seq analysis. A) Global gene expression profile analysis (hPSC-EMSCs (n = 3) Vs. hUC-MSCs (n = 3)) by RNA-seq indicated that hUC-MSCs and hPSC-EMSCs had different gene expression profiles from each other; B) Pearson's correlation analysis of hUC-MSCs and hPSC-EMSCs indicated that EMSCs derived from hPSCs was closely related to the EMSCs derived from hESCs and were distinct from hUC-MSCs. The correlation plot was generated using an online platform ( http://www.bioinformatics.com.cn/ ); C) Comparison between hUC-MSCs and hPSC-EMSCs by MA plot indicated that 1212 genes were differentially upregulated and 716 genes were differentially downregulated in hPSC-EMSCs compared to hUC-MSCs (DEGs, Log 2 Fold Change ≤ -1 or ≥ 1, P adj ≤ 0.05 or > 0.05); D) Heatmap analysis showing the differentially expressed genes associated with ECM, immunomodulation, neuroactivation and axon guidance between hUC-MSCs and hPSC-EMSCs; E) Gene ontology analysis of secretory factors (GO: 0032940) indicated that genes associated with ERK1 and ERK2 cascade were differentially expressed in hUC-MSCs and hPSC-EMSCs; F) ELISA analysis of β-NGF, TGF-β 2 , PDGF-AA and CCL5 in the CM derived from hPSC-EMSCs and hUC-MSCs. Three different EMSC-CM (from H7, H9 and iPSCs) and three UC-CM (from 009, 011 and 013) were used for the analysis. *** and **** represent p < 0.001 and 0.0001 respectively by Student T-test.

    Article Snippet: Enzyme-linked immunosorbent assays (ELISAs) were performed in accordance with the protocols outlined in the Human β Nerve Growth Factor PicoKine™ ELISA Kit, Human TGF-β2 PicoKine™ ELISA Kit, Human PDGF-AA PicoKine™ ELISA Kit and Human Rantes (CCL5) PicoKine TM ELISA Kit (Boster Bio, Pleasanton, CA, USA).

    Techniques: Expressing, RNA Sequencing Assay, Derivative Assay, Generated, Enzyme-linked Immunosorbent Assay

    Rg1’s Effect upon Inflammatory Cytokine Secretion by BMSCs in Aged Rats. The BMSCs were collected on day 5 of passage 3. (A) Interleukin (IL)-6, (B) IL-2, and (C) tumor necrosis factor (TNF)-α. a: comparison with the control group ( p < 0.05); b: comparison with the D-gal-administration group ( p < 0.05). Each error bar represents the standard deviation (SD).

    Journal: BMC Complementary and Alternative Medicine

    Article Title: The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model

    doi: 10.1186/s12906-015-0642-3

    Figure Lengend Snippet: Rg1’s Effect upon Inflammatory Cytokine Secretion by BMSCs in Aged Rats. The BMSCs were collected on day 5 of passage 3. (A) Interleukin (IL)-6, (B) IL-2, and (C) tumor necrosis factor (TNF)-α. a: comparison with the control group ( p < 0.05); b: comparison with the D-gal-administration group ( p < 0.05). Each error bar represents the standard deviation (SD).

    Article Snippet: The IL-2 kit, IL-6 kit, and goat anti-rabbit secondary antibody were purchased from Wuhan Boster Bio-engineering Co., Ltd. (Wuhan, China).

    Techniques: Standard Deviation