human t all cell lines jurkat  (ATCC)


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    ATCC human t all cell lines jurkat
    Human T All Cell Lines Jurkat, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 98 stars, based on 1 article reviews
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    human t all cell lines jurkat - by Bioz Stars, 2022-12
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    ATCC human acute t lymphocytic leukemia cell line
    Human Acute T Lymphocytic Leukemia Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    ATCC jurkat clone e6 1
    Performance of Rapid Amplification of Integration Site without Interference by Genomic DNA contamination (RAISING). All RAISING products were visualized by electrophoresis on 2% agarose gels. Dotted lines in Sanger sequencing spectra indicate the position of transgene integration sites. a The sensitivity of RAISING was assessed by serially diluting TL-Om1 genomic DNA <t>(an</t> <t>HTLV-1-infected</t> <t>cell</t> <t>line</t> harboring a single copy of HTLV-1) with <t>Jurkat</t> genomic DNA (an HTLV-1 <t>negative</t> cell line). Even extremely diluted samples (resulting in PVL as low as 0.032) could be detected by increasing the cycles in the second PCR of RAISING (left panel). Sanger sequencing analysis of RAISING products confirmed that the same integration site was identified in every dilution (right panel). PVL proviral load. b , c Clonality analyses of infected samples using Sanger sequencing (spectra) vs. high throughput sequencing (HTS, pie charts) showed similar results. b HTLV-1 clonality analysis of an asymptomatic carrier (AC) and an adult T-cell <t>leukemia/lymphoma</t> (ATL) patient. c Bovine leukemia virus (BLV) clonality analysis of an aleukemic (AL) cow and a cow with enzootic bovine leukosis (EBL). d Successful amplification of various transgene-integrated fragments (HTLV-1; HIV-1 human immunodeficiency virus, SIV simian immunodeficiency virus, HBV hepatitis B virus, AdV adenovirus, and low-density lipoprotein receptor knock-in mice Ldlr-mLO-4 and Ldlr-mLO-5) with RAISING. e HBV and AdV integration sites. f Clonality analysis of HIV-1 and SIV-infected cells with HTS analysis. HTS read counts indicate the size of each clone. g RAISING with HTS analysis was used to identify on- and off-target effects in Ldlr-mLO-4 and Ldlr-mLO-5 knock-in mice established by genome editing technology. Chr, chromosome.
    Jurkat Clone E6 1, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 98 stars, based on 1 article reviews
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    Performance of Rapid Amplification of Integration Site without Interference by Genomic DNA contamination (RAISING). All RAISING products were visualized by electrophoresis on 2% agarose gels. Dotted lines in Sanger sequencing spectra indicate the position of transgene integration sites. a The sensitivity of RAISING was assessed by serially diluting TL-Om1 genomic DNA (an HTLV-1-infected cell line harboring a single copy of HTLV-1) with Jurkat genomic DNA (an HTLV-1 negative cell line). Even extremely diluted samples (resulting in PVL as low as 0.032) could be detected by increasing the cycles in the second PCR of RAISING (left panel). Sanger sequencing analysis of RAISING products confirmed that the same integration site was identified in every dilution (right panel). PVL proviral load. b , c Clonality analyses of infected samples using Sanger sequencing (spectra) vs. high throughput sequencing (HTS, pie charts) showed similar results. b HTLV-1 clonality analysis of an asymptomatic carrier (AC) and an adult T-cell leukemia/lymphoma (ATL) patient. c Bovine leukemia virus (BLV) clonality analysis of an aleukemic (AL) cow and a cow with enzootic bovine leukosis (EBL). d Successful amplification of various transgene-integrated fragments (HTLV-1; HIV-1 human immunodeficiency virus, SIV simian immunodeficiency virus, HBV hepatitis B virus, AdV adenovirus, and low-density lipoprotein receptor knock-in mice Ldlr-mLO-4 and Ldlr-mLO-5) with RAISING. e HBV and AdV integration sites. f Clonality analysis of HIV-1 and SIV-infected cells with HTS analysis. HTS read counts indicate the size of each clone. g RAISING with HTS analysis was used to identify on- and off-target effects in Ldlr-mLO-4 and Ldlr-mLO-5 knock-in mice established by genome editing technology. Chr, chromosome.

    Journal: Communications Biology

    Article Title: RAISING is a high-performance method for identifying random transgene integration sites

    doi: 10.1038/s42003-022-03467-w

    Figure Lengend Snippet: Performance of Rapid Amplification of Integration Site without Interference by Genomic DNA contamination (RAISING). All RAISING products were visualized by electrophoresis on 2% agarose gels. Dotted lines in Sanger sequencing spectra indicate the position of transgene integration sites. a The sensitivity of RAISING was assessed by serially diluting TL-Om1 genomic DNA (an HTLV-1-infected cell line harboring a single copy of HTLV-1) with Jurkat genomic DNA (an HTLV-1 negative cell line). Even extremely diluted samples (resulting in PVL as low as 0.032) could be detected by increasing the cycles in the second PCR of RAISING (left panel). Sanger sequencing analysis of RAISING products confirmed that the same integration site was identified in every dilution (right panel). PVL proviral load. b , c Clonality analyses of infected samples using Sanger sequencing (spectra) vs. high throughput sequencing (HTS, pie charts) showed similar results. b HTLV-1 clonality analysis of an asymptomatic carrier (AC) and an adult T-cell leukemia/lymphoma (ATL) patient. c Bovine leukemia virus (BLV) clonality analysis of an aleukemic (AL) cow and a cow with enzootic bovine leukosis (EBL). d Successful amplification of various transgene-integrated fragments (HTLV-1; HIV-1 human immunodeficiency virus, SIV simian immunodeficiency virus, HBV hepatitis B virus, AdV adenovirus, and low-density lipoprotein receptor knock-in mice Ldlr-mLO-4 and Ldlr-mLO-5) with RAISING. e HBV and AdV integration sites. f Clonality analysis of HIV-1 and SIV-infected cells with HTS analysis. HTS read counts indicate the size of each clone. g RAISING with HTS analysis was used to identify on- and off-target effects in Ldlr-mLO-4 and Ldlr-mLO-5 knock-in mice established by genome editing technology. Chr, chromosome.

    Article Snippet: The HTLV-1-infected cell lines TL-Om1, LMY2, ED, KK1, and SLB-1; the HTLV-1-negative acute T-cell leukemia cell line Jurkat (Clone E61: ATCC TIB152); the CD4+ human T-cell line PM1 (3038, NIH AIDS Reagent Program); and the cynomolgus macaque T-cell line HSC-F (JCRB1164) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin at 37 °C in a 5% CO2 atmosphere , , – .

    Techniques: Amplification, Electrophoresis, Sequencing, Infection, Polymerase Chain Reaction, Next-Generation Sequencing, Knock-In, Mouse Assay

    Longitudinal analysis of progressors to ATL and non-progressors with clonality value (Cv), HTLV-1 proviral load (PVL), and soluble IL-2 receptor (sIL-2R). a Cv (top), PVL (center), and sIL-2R (bottom) of 15 progressors (red) and 130 non-progressors (black). The time point of ATL onset or last observation visit was set to 0 year 0 month (0Y0M) for progressors or non-progressors, respectively. If multiple data existed in each period of one year, the arithmetic means were displayed. Of 15 progressors, 10 had only data at one-time point before the onset. Data used for the ROC analysis shown in b is indicated by dashed rectangles. b ROC analysis using available data from progressors ( n = 12) and non-progressors ( n = 48) in a time point between one month and one year (0Y1M–1Y0M) before either ATL onset or last observation visit (progressors, median 7.0 months ago; non-progressor, median 6.2 months ago). AUC area under the curve, CI confidence interval.

    Journal: Communications Biology

    Article Title: RAISING is a high-performance method for identifying random transgene integration sites

    doi: 10.1038/s42003-022-03467-w

    Figure Lengend Snippet: Longitudinal analysis of progressors to ATL and non-progressors with clonality value (Cv), HTLV-1 proviral load (PVL), and soluble IL-2 receptor (sIL-2R). a Cv (top), PVL (center), and sIL-2R (bottom) of 15 progressors (red) and 130 non-progressors (black). The time point of ATL onset or last observation visit was set to 0 year 0 month (0Y0M) for progressors or non-progressors, respectively. If multiple data existed in each period of one year, the arithmetic means were displayed. Of 15 progressors, 10 had only data at one-time point before the onset. Data used for the ROC analysis shown in b is indicated by dashed rectangles. b ROC analysis using available data from progressors ( n = 12) and non-progressors ( n = 48) in a time point between one month and one year (0Y1M–1Y0M) before either ATL onset or last observation visit (progressors, median 7.0 months ago; non-progressor, median 6.2 months ago). AUC area under the curve, CI confidence interval.

    Article Snippet: The HTLV-1-infected cell lines TL-Om1, LMY2, ED, KK1, and SLB-1; the HTLV-1-negative acute T-cell leukemia cell line Jurkat (Clone E61: ATCC TIB152); the CD4+ human T-cell line PM1 (3038, NIH AIDS Reagent Program); and the cynomolgus macaque T-cell line HSC-F (JCRB1164) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin at 37 °C in a 5% CO2 atmosphere , , – .

    Techniques:

    Clinical utility of clonality analysis using Rapid Amplification of Integration Site without Interference by Genomic DNA contamination (RAISING) and Clonality Value (CLOVA) software. HTLV-1 proviral load (PVL, a ) and clonality values (Cv, b ) of peripheral blood samples from asymptomatic carriers (AC, n = 201, black), HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP, n = 223, blue), and adult T-cell leukemia/lymphoma (ATL, n = 264, red) patients. The median PVL and Cv in each group and subtype are shown as horizontal lines. p -value were calculated using Dunn’s multiple comparisons test. **** p

    Journal: Communications Biology

    Article Title: RAISING is a high-performance method for identifying random transgene integration sites

    doi: 10.1038/s42003-022-03467-w

    Figure Lengend Snippet: Clinical utility of clonality analysis using Rapid Amplification of Integration Site without Interference by Genomic DNA contamination (RAISING) and Clonality Value (CLOVA) software. HTLV-1 proviral load (PVL, a ) and clonality values (Cv, b ) of peripheral blood samples from asymptomatic carriers (AC, n = 201, black), HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP, n = 223, blue), and adult T-cell leukemia/lymphoma (ATL, n = 264, red) patients. The median PVL and Cv in each group and subtype are shown as horizontal lines. p -value were calculated using Dunn’s multiple comparisons test. **** p

    Article Snippet: The HTLV-1-infected cell lines TL-Om1, LMY2, ED, KK1, and SLB-1; the HTLV-1-negative acute T-cell leukemia cell line Jurkat (Clone E61: ATCC TIB152); the CD4+ human T-cell line PM1 (3038, NIH AIDS Reagent Program); and the cynomolgus macaque T-cell line HSC-F (JCRB1164) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin at 37 °C in a 5% CO2 atmosphere , , – .

    Techniques: Amplification, Software

    Characterization of Rapid Amplification of Integration Site (RAIS) and RAIS without Interference by Genomic DNA contamination (RAISING). a Schematic representation of RAIS and RAISING. Step 1: Single-stranded DNA (ssDNA) synthesis; Step 2: Column purification of ssDNA; Step 3: polyA-tailing and polyAG-tailing of ssDNA in RAIS and RAISING, respectively; Step 4: Double-stranded DNA (dsDNA) synthesis; Step 5: DNA purification of dsDNA with magnetic streptavidin-beads in RAIS and first PCR in RAISING; Step 6: First PCR in RAIS and second PCR in RAISING; Step 7: Second PCR in RAIS and Sanger sequencing or high throughput sequencing (HTS) library preparation in RAISING b – d , Linear-amplification, accuracy, and consistency between RAIS and RAISING. Genomic DNA of LMY2 and ED cell lines, each harboring a single HTLV-1 integration site, were mixed at the indicated percentages, and these mixed samples were processed using both RAIS and RAISING. b Products were visualized by electrophoresis on 2% agarose gels. c Products were analyzed using Sanger sequencing, with dotted lines indicating the position of the HTLV-1 integration sites. d Products were analyzed using HTS to measure clone size. Data from two independent experiments (1 and 2) are shown.

    Journal: Communications Biology

    Article Title: RAISING is a high-performance method for identifying random transgene integration sites

    doi: 10.1038/s42003-022-03467-w

    Figure Lengend Snippet: Characterization of Rapid Amplification of Integration Site (RAIS) and RAIS without Interference by Genomic DNA contamination (RAISING). a Schematic representation of RAIS and RAISING. Step 1: Single-stranded DNA (ssDNA) synthesis; Step 2: Column purification of ssDNA; Step 3: polyA-tailing and polyAG-tailing of ssDNA in RAIS and RAISING, respectively; Step 4: Double-stranded DNA (dsDNA) synthesis; Step 5: DNA purification of dsDNA with magnetic streptavidin-beads in RAIS and first PCR in RAISING; Step 6: First PCR in RAIS and second PCR in RAISING; Step 7: Second PCR in RAIS and Sanger sequencing or high throughput sequencing (HTS) library preparation in RAISING b – d , Linear-amplification, accuracy, and consistency between RAIS and RAISING. Genomic DNA of LMY2 and ED cell lines, each harboring a single HTLV-1 integration site, were mixed at the indicated percentages, and these mixed samples were processed using both RAIS and RAISING. b Products were visualized by electrophoresis on 2% agarose gels. c Products were analyzed using Sanger sequencing, with dotted lines indicating the position of the HTLV-1 integration sites. d Products were analyzed using HTS to measure clone size. Data from two independent experiments (1 and 2) are shown.

    Article Snippet: The HTLV-1-infected cell lines TL-Om1, LMY2, ED, KK1, and SLB-1; the HTLV-1-negative acute T-cell leukemia cell line Jurkat (Clone E61: ATCC TIB152); the CD4+ human T-cell line PM1 (3038, NIH AIDS Reagent Program); and the cynomolgus macaque T-cell line HSC-F (JCRB1164) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin at 37 °C in a 5% CO2 atmosphere , , – .

    Techniques: Amplification, Purification, DNA Purification, Polymerase Chain Reaction, Sequencing, Next-Generation Sequencing, Electrophoresis

    PODXL decreases the expression of CD86, MHC-I and MHC-II in APCs. (A) Raji-PODXL, Raji-Ctrl cells and CMTMR-labeled Jurkat cells were preincubated separately with a blocking anti-LFA-1 monoclonal antibody for 15 min. Afterwards, Raji-PODXL and Raji-Ctrl were mixed with CMTMR-labeled Jurkat cells at a ratio of 1:1 and incubated for 30 min. The percentage of Raji-Jurkat cell conjugates was determined by flow cytometry as in ( Figure 4A ). Bar graph represents the mean ± SD of nine independent experiments. *p

    Journal: Frontiers in Immunology

    Article Title: Podocalyxin Expressed in Antigen Presenting Cells Promotes Interaction With T Cells and Alters Centrosome Translocation to the Contact Site

    doi: 10.3389/fimmu.2022.835527

    Figure Lengend Snippet: PODXL decreases the expression of CD86, MHC-I and MHC-II in APCs. (A) Raji-PODXL, Raji-Ctrl cells and CMTMR-labeled Jurkat cells were preincubated separately with a blocking anti-LFA-1 monoclonal antibody for 15 min. Afterwards, Raji-PODXL and Raji-Ctrl were mixed with CMTMR-labeled Jurkat cells at a ratio of 1:1 and incubated for 30 min. The percentage of Raji-Jurkat cell conjugates was determined by flow cytometry as in ( Figure 4A ). Bar graph represents the mean ± SD of nine independent experiments. *p

    Article Snippet: THP-1 (acute monocytic leukemia), K562 (chronic myelogenous leukemia), HL-60 (acute promyelocytic leukemia), KG1 (acute myelogenous leukemia), Raji (Burkitt lymphoma), and Jurkat (acute T cell leukemia) human cell lines were obtained from American Type Culture Collection (ATCC).

    Techniques: Expressing, Labeling, Blocking Assay, Incubation, Flow Cytometry