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(A) Immunofluorescent staining of frozen lung sections of MHV68 infected IFNγR−/− mice at day 7. Virus antigen detection was performed using an anti-MHV68 polyclonal antibody (green). Type II cells were detected using anti-pro-SP-C antibody (red). Sections were counterstained with DAPI (blue). Merged image shows yellow cells indicating type II cells that support lytic infection of the virus (arrows). Magnification ×800. Staining is representative of 5 different animals. (B) Immunostaining of frozen lung sections of IFNγR−/− mice at day 7 using a monoclonal antibody against the virus ORF59 (red) and the anti-FSP-1 antibody (green). Merged image shows nuclear localization of viral antigen in FSP-1 positive cells (arrows). Magnification ×400. Staining is representative of 3 different animals. (C) Dual IHC on formalin-fixed paraffin-embedded lung sections of MHV68 infected IFNγR−/− mice at day 120. Epithelial cells expressing mesenchymal cell markers (arrow) were detected using anti-TTF-1 (brown nuclei) and anti-FSP-1 (red cytoplasm) antibodies. Magnification ×400. Box shows area depicted with magnification ×1000. Staining is representative of 5 different animals. (D) Dual immunofluorescence staining in frozen lung sections of MHV68 infected IFNγR−/− mice at day <t>120</t> <t>using</t> <t>anti-pro-SP-B</t> (red) and anti-N-cadherin (green) antibodies. Yellow cells indicate type II cells expressing N-cadherin mesenchymal cell marker (arrows). Nuclei were visualized by DAPI staining (blue). Magnification ×200. Staining is representative of 5 different animals.
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Image Search Results


KEY RESOURCES TABLE

Journal: Cell

Article Title: Human inherited CCR2 deficiency underlies progressive polycystic lung disease

doi: 10.1016/j.cell.2023.11.036

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human SP-B , Seven Hills Bioreagents , Cat# WRAB-55522; RRID: AB_2938816.

Techniques: Virus, Recombinant, Staining, Imaging, Enzyme-linked Immunosorbent Assay, Isolation, Sequencing, Expressing, Subcloning, Software, Membrane

(A) Immunofluorescent staining of frozen lung sections of MHV68 infected IFNγR−/− mice at day 7. Virus antigen detection was performed using an anti-MHV68 polyclonal antibody (green). Type II cells were detected using anti-pro-SP-C antibody (red). Sections were counterstained with DAPI (blue). Merged image shows yellow cells indicating type II cells that support lytic infection of the virus (arrows). Magnification ×800. Staining is representative of 5 different animals. (B) Immunostaining of frozen lung sections of IFNγR−/− mice at day 7 using a monoclonal antibody against the virus ORF59 (red) and the anti-FSP-1 antibody (green). Merged image shows nuclear localization of viral antigen in FSP-1 positive cells (arrows). Magnification ×400. Staining is representative of 3 different animals. (C) Dual IHC on formalin-fixed paraffin-embedded lung sections of MHV68 infected IFNγR−/− mice at day 120. Epithelial cells expressing mesenchymal cell markers (arrow) were detected using anti-TTF-1 (brown nuclei) and anti-FSP-1 (red cytoplasm) antibodies. Magnification ×400. Box shows area depicted with magnification ×1000. Staining is representative of 5 different animals. (D) Dual immunofluorescence staining in frozen lung sections of MHV68 infected IFNγR−/− mice at day 120 using anti-pro-SP-B (red) and anti-N-cadherin (green) antibodies. Yellow cells indicate type II cells expressing N-cadherin mesenchymal cell marker (arrows). Nuclei were visualized by DAPI staining (blue). Magnification ×200. Staining is representative of 5 different animals.

Journal: PLoS ONE

Article Title: Twist: A Regulator of Epithelial-Mesenchymal Transition in Lung Fibrosis

doi: 10.1371/journal.pone.0007559

Figure Lengend Snippet: (A) Immunofluorescent staining of frozen lung sections of MHV68 infected IFNγR−/− mice at day 7. Virus antigen detection was performed using an anti-MHV68 polyclonal antibody (green). Type II cells were detected using anti-pro-SP-C antibody (red). Sections were counterstained with DAPI (blue). Merged image shows yellow cells indicating type II cells that support lytic infection of the virus (arrows). Magnification ×800. Staining is representative of 5 different animals. (B) Immunostaining of frozen lung sections of IFNγR−/− mice at day 7 using a monoclonal antibody against the virus ORF59 (red) and the anti-FSP-1 antibody (green). Merged image shows nuclear localization of viral antigen in FSP-1 positive cells (arrows). Magnification ×400. Staining is representative of 3 different animals. (C) Dual IHC on formalin-fixed paraffin-embedded lung sections of MHV68 infected IFNγR−/− mice at day 120. Epithelial cells expressing mesenchymal cell markers (arrow) were detected using anti-TTF-1 (brown nuclei) and anti-FSP-1 (red cytoplasm) antibodies. Magnification ×400. Box shows area depicted with magnification ×1000. Staining is representative of 5 different animals. (D) Dual immunofluorescence staining in frozen lung sections of MHV68 infected IFNγR−/− mice at day 120 using anti-pro-SP-B (red) and anti-N-cadherin (green) antibodies. Yellow cells indicate type II cells expressing N-cadherin mesenchymal cell marker (arrows). Nuclei were visualized by DAPI staining (blue). Magnification ×200. Staining is representative of 5 different animals.

Article Snippet: Polyclonal rabbit anti-human pro-SP-C (Chemicon International); mouse monoclonal anti-human pro-SP-B (Thermo Fisher Scientific); rabbit polyclonal anti-S100A4 (anti-FSP-1) (Dako Cytomation); mouse monoclonal anti-TTF-1 (Novocastra); rabbit polyclonal anti-Twist (sc-15393, Santa Cruz Biotechnology, Inc); mouse monoclonal anti-Twist (sc-81417, Santa Cruz Biotechnology, Inc); mouse monoclonal anti-βactin (Sigma-Aldrich, Saint Louis, MO); rabbit polyclonal anti-E-cadherin (Santa Cruz Biotechnology, Inc); rabbit polyclonal anti-N-cadherin (Abcam); rabbit polyclonal anti-Collagen I (Millipore); rabbit polyclonal anti-Collagen IV (Santa Cruz Biotech Inc); goat polyclonal anti-Vimentin (Chemicon International); rabbit polyclonal anti-Occludin (Zymed Laboratories); rabbit polyclonal anti-Fibronectin (Sigma) antibodies for immunofluorescence and immunoblot assays were used according to the manufacturer recommendations.

Techniques: Staining, Infection, Immunostaining, Formalin-fixed Paraffin-Embedded, Expressing, Immunofluorescence, Marker

(A) Immunofluorescent staining of lung frozen sections of IPF lung using anti-N-cadherin (green) and anti-pro-SP-B (red) antibodies. Type II cells were detected by Pro-SP-B staining (red, open arrow). Yellow cells indicate type II cells expressing the mesenchymal cell marker N-cadherin (closed arrows). Scattered green cells were observed intercalating red and yellow type II epithelial cells (asterisks). Magnification ×100. Box shows area depicted at magnification ×1000 in (B). Nuclei were visualized by DAPI staining (blue). Stainings are representative of 3 independent assays in lung tissues from 3 IPF patients.

Journal: PLoS ONE

Article Title: Twist: A Regulator of Epithelial-Mesenchymal Transition in Lung Fibrosis

doi: 10.1371/journal.pone.0007559

Figure Lengend Snippet: (A) Immunofluorescent staining of lung frozen sections of IPF lung using anti-N-cadherin (green) and anti-pro-SP-B (red) antibodies. Type II cells were detected by Pro-SP-B staining (red, open arrow). Yellow cells indicate type II cells expressing the mesenchymal cell marker N-cadherin (closed arrows). Scattered green cells were observed intercalating red and yellow type II epithelial cells (asterisks). Magnification ×100. Box shows area depicted at magnification ×1000 in (B). Nuclei were visualized by DAPI staining (blue). Stainings are representative of 3 independent assays in lung tissues from 3 IPF patients.

Article Snippet: Polyclonal rabbit anti-human pro-SP-C (Chemicon International); mouse monoclonal anti-human pro-SP-B (Thermo Fisher Scientific); rabbit polyclonal anti-S100A4 (anti-FSP-1) (Dako Cytomation); mouse monoclonal anti-TTF-1 (Novocastra); rabbit polyclonal anti-Twist (sc-15393, Santa Cruz Biotechnology, Inc); mouse monoclonal anti-Twist (sc-81417, Santa Cruz Biotechnology, Inc); mouse monoclonal anti-βactin (Sigma-Aldrich, Saint Louis, MO); rabbit polyclonal anti-E-cadherin (Santa Cruz Biotechnology, Inc); rabbit polyclonal anti-N-cadherin (Abcam); rabbit polyclonal anti-Collagen I (Millipore); rabbit polyclonal anti-Collagen IV (Santa Cruz Biotech Inc); goat polyclonal anti-Vimentin (Chemicon International); rabbit polyclonal anti-Occludin (Zymed Laboratories); rabbit polyclonal anti-Fibronectin (Sigma) antibodies for immunofluorescence and immunoblot assays were used according to the manufacturer recommendations.

Techniques: Staining, Expressing, Marker