Journal: Heliyon
Article Title: CXCL12-loaded-hydrogel (CLG): A new device for metastatic circulating tumor cells (CTCs) capturing and characterization
doi: 10.1016/j.heliyon.2024.e35524
Figure Lengend Snippet: Human cancer cells efficiently infiltrate CLG. (A) Schematic representation of Gel infiltration assay. (B) Human colon, renal, lung, and ovarian cells (HT29, A498, H460 and OVCAR8) were seeded on empty (EG) or CXCL12-loaded Hydrogel (CLG) on 8-well chamber slide in Serum Free (SF) media and allowed to infiltrate 16 h. Cells were fixed with 2%FA + Hyaluronidase (HAase) for 6 h and stained with DAPI for enumeration at fluorescent microscope. B 100, 500 or 1000 A498, HT29, H460 and OVCAR8 were allowed to infiltrate EG or CLG. Data in bar graphs represent mean ± SEM of at least two independent experiments. A two-tailed t -test was used to assess significance. *P < 0·05, **P < 0·01 ***P < 0·001.
Article Snippet: Human Renal cancer cells A498 were cultured in Iscove's Modified Dulbecco's Medium (IMDM) (Gibco BRL, Grand Island, NY).
Techniques: Staining, Microscopy, Two Tailed Test