renal cell cancer cell lines 786 o  (ATCC)


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    ATCC renal cell cancer cell lines 786 o
    Renal Cell Cancer Cell Lines 786 O, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human renal cancer cell line 786 o  (ATCC)


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    ATCC human renal cancer cell line 786 o
    Human Renal Cancer Cell Line 786 O, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    renal cell cancer cell lines 786 o  (ATCC)


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    ATCC renal cell cancer cell lines 786 o
    Renal Cell Cancer Cell Lines 786 O, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human renal cancer cell lines 786 o  (ATCC)


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    ATCC human renal cancer cell lines 786 o
    Human Renal Cancer Cell Lines 786 O, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    786 o human renal cancer cell lines  (ATCC)


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    ATCC 786 o human renal cancer cell lines
    miR-21 and PPAR-α expressions in HK-2 and renal cancer cell lines before confluence (BC) and two days after confluence (C + 2). ( A ) Using RT-qPCR, miR-21 expression was determined in the <t>786-O,</t> ACHN, RCC10, and RCC4 renal cancer cells as compared to normal HK-2 renal epithelial cells. RNU48 was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001). ( B ) Western blotting was performed on whole cell extracts. Antibodies against PPAR-α and β-actin were used.
    786 O Human Renal Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A Double-Negative Feedback Interaction between miR-21 and PPAR-α in Clear Renal Cell Carcinoma"

    Article Title: A Double-Negative Feedback Interaction between miR-21 and PPAR-α in Clear Renal Cell Carcinoma

    Journal: Cancers

    doi: 10.3390/cancers14030795

    miR-21 and PPAR-α expressions in HK-2 and renal cancer cell lines before confluence (BC) and two days after confluence (C + 2). ( A ) Using RT-qPCR, miR-21 expression was determined in the 786-O, ACHN, RCC10, and RCC4 renal cancer cells as compared to normal HK-2 renal epithelial cells. RNU48 was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001). ( B ) Western blotting was performed on whole cell extracts. Antibodies against PPAR-α and β-actin were used.
    Figure Legend Snippet: miR-21 and PPAR-α expressions in HK-2 and renal cancer cell lines before confluence (BC) and two days after confluence (C + 2). ( A ) Using RT-qPCR, miR-21 expression was determined in the 786-O, ACHN, RCC10, and RCC4 renal cancer cells as compared to normal HK-2 renal epithelial cells. RNU48 was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001). ( B ) Western blotting was performed on whole cell extracts. Antibodies against PPAR-α and β-actin were used.

    Techniques Used: Quantitative RT-PCR, Expressing, Western Blot

    PPAR-α expression and/or activation decreases miR-21 expression and transcription. ( A ) ACHN and 786-O cells were transfected with pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors. At 24 h after transfection, the cells were incubated with 0, 400 nM, or 1 μM of GW7647. At 48 h after transfection, miR-21 expression was determined by qPCR. RNU48 was used as an internal control. ( B ) ACHN and 786-O cells were co-transfected with miR-21-Luc promoter and pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors. At 24 h after transfection, the cells were incubated with 0 or 400 nM of GW7647. At 48 h after transfection, luciferase activity was determined. The values obtained with the empty vector were referred to as 1. The values are means ± SEM and represent at least four separate experiments (* p < 0.05 and ** p < 0.01).
    Figure Legend Snippet: PPAR-α expression and/or activation decreases miR-21 expression and transcription. ( A ) ACHN and 786-O cells were transfected with pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors. At 24 h after transfection, the cells were incubated with 0, 400 nM, or 1 μM of GW7647. At 48 h after transfection, miR-21 expression was determined by qPCR. RNU48 was used as an internal control. ( B ) ACHN and 786-O cells were co-transfected with miR-21-Luc promoter and pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors. At 24 h after transfection, the cells were incubated with 0 or 400 nM of GW7647. At 48 h after transfection, luciferase activity was determined. The values obtained with the empty vector were referred to as 1. The values are means ± SEM and represent at least four separate experiments (* p < 0.05 and ** p < 0.01).

    Techniques Used: Expressing, Activation Assay, Transfection, Plasmid Preparation, Incubation, Luciferase, Activity Assay

    PPAR-α expression and/or activation decreases AP-1 and NF-κB transcriptional activity and binding to miR-21 promoter. ( A ) ACHN and 786-O cells were co-transfected with pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors and κB-Luc or AP1-Luc synthetic promoters. At 24 h after transfection, the cells were incubated with 0 or 400 nM of GW7647. At 48 h after transfection, luciferase activity was measured. Luciferase activity in cells transfected with pSG5-EV was set as 1. ( B , C ) Soluble chromatin from the ACHN and 786-O cells transfected with pSG5-EV or pSG5-PPAR-α expression vectors was immunoprecipitated with control immunoglobulin G (IgG) and anti-c-jun ( B ) or anti-NF-κB p65 antibodies ( C ). The precipitated DNA samples were amplified by PCR with pairs of primers flanking the binding site regions for AP-1 and NF-κB. The results were expressed as the percentage of input. The values obtained with the empty vector were referred to as 1. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001).
    Figure Legend Snippet: PPAR-α expression and/or activation decreases AP-1 and NF-κB transcriptional activity and binding to miR-21 promoter. ( A ) ACHN and 786-O cells were co-transfected with pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors and κB-Luc or AP1-Luc synthetic promoters. At 24 h after transfection, the cells were incubated with 0 or 400 nM of GW7647. At 48 h after transfection, luciferase activity was measured. Luciferase activity in cells transfected with pSG5-EV was set as 1. ( B , C ) Soluble chromatin from the ACHN and 786-O cells transfected with pSG5-EV or pSG5-PPAR-α expression vectors was immunoprecipitated with control immunoglobulin G (IgG) and anti-c-jun ( B ) or anti-NF-κB p65 antibodies ( C ). The precipitated DNA samples were amplified by PCR with pairs of primers flanking the binding site regions for AP-1 and NF-κB. The results were expressed as the percentage of input. The values obtained with the empty vector were referred to as 1. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Techniques Used: Expressing, Activation Assay, Activity Assay, Binding Assay, Transfection, Plasmid Preparation, Incubation, Luciferase, Immunoprecipitation, Amplification

    PPAR-α expression and/or activation increases the expression of two miR-21 targets: PDCD4 and PTEN in ACHN ( A ) and 786-O cells ( B ). Western blot performed on cell extracts obtained from ACHN and 786-O transfected with pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors and treated for 24h after transfection with 0, 400 nM, or 1 μM of GW7647. The intensities of the signal were determined by densitometric scanning, expressed as the relative signal PDCP4/Actin and PTEN/Actin ratios and represented as histograms. Expression in the “pSG5-EV with no treatment” condition was arbitrarily set to 1.
    Figure Legend Snippet: PPAR-α expression and/or activation increases the expression of two miR-21 targets: PDCD4 and PTEN in ACHN ( A ) and 786-O cells ( B ). Western blot performed on cell extracts obtained from ACHN and 786-O transfected with pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors and treated for 24h after transfection with 0, 400 nM, or 1 μM of GW7647. The intensities of the signal were determined by densitometric scanning, expressed as the relative signal PDCP4/Actin and PTEN/Actin ratios and represented as histograms. Expression in the “pSG5-EV with no treatment” condition was arbitrarily set to 1.

    Techniques Used: Expressing, Activation Assay, Western Blot, Transfection, Plasmid Preparation

    Inhibition of miR-21 expression increases PPAR-α and PPAR-α target gene expressions in 786-O cells. ( A ) Western blot performed on cell extracts obtained from 786-O cells transfected for 24h with 10 nM of LNA-C, LNA-anti-miR-21 (LNA-21), pre-miR-neg, or pre-miR-21. ( B ) The 786-O cells were co-transfected with J6-PPRE-TK-Luc reporter plasmid and 10 nM of LNA-C or LNA-anti-miR-21 (LNA-21). At 48 h after transfection, luciferase activity was measured. Luciferase activity in the cells co-transfected with J6-PPRE-TK-Luc and LNA-C was set as 1. ( C ) The 786-O cells were co-transfected with J6-PPRE-TK-Luc reporter plasmid and 10 nM of pre-miR-neg or pre-miR-21. At 48 h after transfection, luciferase activity was measured. Luciferase activity in the cells co-transfected with J6-PPRE-TK-Luc and pre-miR-neg was set as 1. ( D ) The 786-O cells were transfected with 10 nM of LNA-C or LNA-anti-miR-21 (LNA-21). At 48 h after transfection, SLC22A5, CPT1, and ACOX1 expressions were determined by RT-qPCR. PPIA was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001).
    Figure Legend Snippet: Inhibition of miR-21 expression increases PPAR-α and PPAR-α target gene expressions in 786-O cells. ( A ) Western blot performed on cell extracts obtained from 786-O cells transfected for 24h with 10 nM of LNA-C, LNA-anti-miR-21 (LNA-21), pre-miR-neg, or pre-miR-21. ( B ) The 786-O cells were co-transfected with J6-PPRE-TK-Luc reporter plasmid and 10 nM of LNA-C or LNA-anti-miR-21 (LNA-21). At 48 h after transfection, luciferase activity was measured. Luciferase activity in the cells co-transfected with J6-PPRE-TK-Luc and LNA-C was set as 1. ( C ) The 786-O cells were co-transfected with J6-PPRE-TK-Luc reporter plasmid and 10 nM of pre-miR-neg or pre-miR-21. At 48 h after transfection, luciferase activity was measured. Luciferase activity in the cells co-transfected with J6-PPRE-TK-Luc and pre-miR-neg was set as 1. ( D ) The 786-O cells were transfected with 10 nM of LNA-C or LNA-anti-miR-21 (LNA-21). At 48 h after transfection, SLC22A5, CPT1, and ACOX1 expressions were determined by RT-qPCR. PPIA was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Techniques Used: Inhibition, Expressing, Western Blot, Transfection, Plasmid Preparation, Luciferase, Activity Assay, Quantitative RT-PCR

    human renal cancer cell line 786 o  (ATCC)


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    ATCC human renal cancer cell line 786 o
    Human Renal Cancer Cell Line 786 O, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cancer cell lines 786 o  (ATCC)


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    ATCC cancer cell lines 786 o
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    ATCC human renal cancer cell lines 786 o
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    ATCC human renal cancer cell line 786 o
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    ATCC human renal cancer cell line 786 o
    Human Renal Cancer Cell Line 786 O, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    renal cancer cell lines 786 o  (ATCC)


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    ATCC renal cancer cell lines 786 o
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    ATCC renal cell cancer cell lines 786 o
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    ATCC 786 o human renal cancer cell lines
    miR-21 and PPAR-α expressions in HK-2 and renal cancer cell lines before confluence (BC) and two days after confluence (C + 2). ( A ) Using RT-qPCR, miR-21 expression was determined in the <t>786-O,</t> ACHN, RCC10, and RCC4 renal cancer cells as compared to normal HK-2 renal epithelial cells. RNU48 was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001). ( B ) Western blotting was performed on whole cell extracts. Antibodies against PPAR-α and β-actin were used.
    786 O Human Renal Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC cancer cell lines 786 o
    miR-21 and PPAR-α expressions in HK-2 and renal cancer cell lines before confluence (BC) and two days after confluence (C + 2). ( A ) Using RT-qPCR, miR-21 expression was determined in the <t>786-O,</t> ACHN, RCC10, and RCC4 renal cancer cells as compared to normal HK-2 renal epithelial cells. RNU48 was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001). ( B ) Western blotting was performed on whole cell extracts. Antibodies against PPAR-α and β-actin were used.
    Cancer Cell Lines 786 O, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC renal cancer cell lines 786 o
    miR-21 and PPAR-α expressions in HK-2 and renal cancer cell lines before confluence (BC) and two days after confluence (C + 2). ( A ) Using RT-qPCR, miR-21 expression was determined in the <t>786-O,</t> ACHN, RCC10, and RCC4 renal cancer cells as compared to normal HK-2 renal epithelial cells. RNU48 was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001). ( B ) Western blotting was performed on whole cell extracts. Antibodies against PPAR-α and β-actin were used.
    Renal Cancer Cell Lines 786 O, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    miR-21 and PPAR-α expressions in HK-2 and renal cancer cell lines before confluence (BC) and two days after confluence (C + 2). ( A ) Using RT-qPCR, miR-21 expression was determined in the 786-O, ACHN, RCC10, and RCC4 renal cancer cells as compared to normal HK-2 renal epithelial cells. RNU48 was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001). ( B ) Western blotting was performed on whole cell extracts. Antibodies against PPAR-α and β-actin were used.

    Journal: Cancers

    Article Title: A Double-Negative Feedback Interaction between miR-21 and PPAR-α in Clear Renal Cell Carcinoma

    doi: 10.3390/cancers14030795

    Figure Lengend Snippet: miR-21 and PPAR-α expressions in HK-2 and renal cancer cell lines before confluence (BC) and two days after confluence (C + 2). ( A ) Using RT-qPCR, miR-21 expression was determined in the 786-O, ACHN, RCC10, and RCC4 renal cancer cells as compared to normal HK-2 renal epithelial cells. RNU48 was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001). ( B ) Western blotting was performed on whole cell extracts. Antibodies against PPAR-α and β-actin were used.

    Article Snippet: The HK-2 human proximal tubule epithelial cell line, ACHN, and 786-O human renal cancer cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Quantitative RT-PCR, Expressing, Western Blot

    PPAR-α expression and/or activation decreases miR-21 expression and transcription. ( A ) ACHN and 786-O cells were transfected with pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors. At 24 h after transfection, the cells were incubated with 0, 400 nM, or 1 μM of GW7647. At 48 h after transfection, miR-21 expression was determined by qPCR. RNU48 was used as an internal control. ( B ) ACHN and 786-O cells were co-transfected with miR-21-Luc promoter and pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors. At 24 h after transfection, the cells were incubated with 0 or 400 nM of GW7647. At 48 h after transfection, luciferase activity was determined. The values obtained with the empty vector were referred to as 1. The values are means ± SEM and represent at least four separate experiments (* p < 0.05 and ** p < 0.01).

    Journal: Cancers

    Article Title: A Double-Negative Feedback Interaction between miR-21 and PPAR-α in Clear Renal Cell Carcinoma

    doi: 10.3390/cancers14030795

    Figure Lengend Snippet: PPAR-α expression and/or activation decreases miR-21 expression and transcription. ( A ) ACHN and 786-O cells were transfected with pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors. At 24 h after transfection, the cells were incubated with 0, 400 nM, or 1 μM of GW7647. At 48 h after transfection, miR-21 expression was determined by qPCR. RNU48 was used as an internal control. ( B ) ACHN and 786-O cells were co-transfected with miR-21-Luc promoter and pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors. At 24 h after transfection, the cells were incubated with 0 or 400 nM of GW7647. At 48 h after transfection, luciferase activity was determined. The values obtained with the empty vector were referred to as 1. The values are means ± SEM and represent at least four separate experiments (* p < 0.05 and ** p < 0.01).

    Article Snippet: The HK-2 human proximal tubule epithelial cell line, ACHN, and 786-O human renal cancer cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Expressing, Activation Assay, Transfection, Plasmid Preparation, Incubation, Luciferase, Activity Assay

    PPAR-α expression and/or activation decreases AP-1 and NF-κB transcriptional activity and binding to miR-21 promoter. ( A ) ACHN and 786-O cells were co-transfected with pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors and κB-Luc or AP1-Luc synthetic promoters. At 24 h after transfection, the cells were incubated with 0 or 400 nM of GW7647. At 48 h after transfection, luciferase activity was measured. Luciferase activity in cells transfected with pSG5-EV was set as 1. ( B , C ) Soluble chromatin from the ACHN and 786-O cells transfected with pSG5-EV or pSG5-PPAR-α expression vectors was immunoprecipitated with control immunoglobulin G (IgG) and anti-c-jun ( B ) or anti-NF-κB p65 antibodies ( C ). The precipitated DNA samples were amplified by PCR with pairs of primers flanking the binding site regions for AP-1 and NF-κB. The results were expressed as the percentage of input. The values obtained with the empty vector were referred to as 1. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Journal: Cancers

    Article Title: A Double-Negative Feedback Interaction between miR-21 and PPAR-α in Clear Renal Cell Carcinoma

    doi: 10.3390/cancers14030795

    Figure Lengend Snippet: PPAR-α expression and/or activation decreases AP-1 and NF-κB transcriptional activity and binding to miR-21 promoter. ( A ) ACHN and 786-O cells were co-transfected with pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors and κB-Luc or AP1-Luc synthetic promoters. At 24 h after transfection, the cells were incubated with 0 or 400 nM of GW7647. At 48 h after transfection, luciferase activity was measured. Luciferase activity in cells transfected with pSG5-EV was set as 1. ( B , C ) Soluble chromatin from the ACHN and 786-O cells transfected with pSG5-EV or pSG5-PPAR-α expression vectors was immunoprecipitated with control immunoglobulin G (IgG) and anti-c-jun ( B ) or anti-NF-κB p65 antibodies ( C ). The precipitated DNA samples were amplified by PCR with pairs of primers flanking the binding site regions for AP-1 and NF-κB. The results were expressed as the percentage of input. The values obtained with the empty vector were referred to as 1. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Article Snippet: The HK-2 human proximal tubule epithelial cell line, ACHN, and 786-O human renal cancer cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Expressing, Activation Assay, Activity Assay, Binding Assay, Transfection, Plasmid Preparation, Incubation, Luciferase, Immunoprecipitation, Amplification

    PPAR-α expression and/or activation increases the expression of two miR-21 targets: PDCD4 and PTEN in ACHN ( A ) and 786-O cells ( B ). Western blot performed on cell extracts obtained from ACHN and 786-O transfected with pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors and treated for 24h after transfection with 0, 400 nM, or 1 μM of GW7647. The intensities of the signal were determined by densitometric scanning, expressed as the relative signal PDCP4/Actin and PTEN/Actin ratios and represented as histograms. Expression in the “pSG5-EV with no treatment” condition was arbitrarily set to 1.

    Journal: Cancers

    Article Title: A Double-Negative Feedback Interaction between miR-21 and PPAR-α in Clear Renal Cell Carcinoma

    doi: 10.3390/cancers14030795

    Figure Lengend Snippet: PPAR-α expression and/or activation increases the expression of two miR-21 targets: PDCD4 and PTEN in ACHN ( A ) and 786-O cells ( B ). Western blot performed on cell extracts obtained from ACHN and 786-O transfected with pSG5-EV (empty vector) or pSG5-PPAR-α expression vectors and treated for 24h after transfection with 0, 400 nM, or 1 μM of GW7647. The intensities of the signal were determined by densitometric scanning, expressed as the relative signal PDCP4/Actin and PTEN/Actin ratios and represented as histograms. Expression in the “pSG5-EV with no treatment” condition was arbitrarily set to 1.

    Article Snippet: The HK-2 human proximal tubule epithelial cell line, ACHN, and 786-O human renal cancer cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Expressing, Activation Assay, Western Blot, Transfection, Plasmid Preparation

    Inhibition of miR-21 expression increases PPAR-α and PPAR-α target gene expressions in 786-O cells. ( A ) Western blot performed on cell extracts obtained from 786-O cells transfected for 24h with 10 nM of LNA-C, LNA-anti-miR-21 (LNA-21), pre-miR-neg, or pre-miR-21. ( B ) The 786-O cells were co-transfected with J6-PPRE-TK-Luc reporter plasmid and 10 nM of LNA-C or LNA-anti-miR-21 (LNA-21). At 48 h after transfection, luciferase activity was measured. Luciferase activity in the cells co-transfected with J6-PPRE-TK-Luc and LNA-C was set as 1. ( C ) The 786-O cells were co-transfected with J6-PPRE-TK-Luc reporter plasmid and 10 nM of pre-miR-neg or pre-miR-21. At 48 h after transfection, luciferase activity was measured. Luciferase activity in the cells co-transfected with J6-PPRE-TK-Luc and pre-miR-neg was set as 1. ( D ) The 786-O cells were transfected with 10 nM of LNA-C or LNA-anti-miR-21 (LNA-21). At 48 h after transfection, SLC22A5, CPT1, and ACOX1 expressions were determined by RT-qPCR. PPIA was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Journal: Cancers

    Article Title: A Double-Negative Feedback Interaction between miR-21 and PPAR-α in Clear Renal Cell Carcinoma

    doi: 10.3390/cancers14030795

    Figure Lengend Snippet: Inhibition of miR-21 expression increases PPAR-α and PPAR-α target gene expressions in 786-O cells. ( A ) Western blot performed on cell extracts obtained from 786-O cells transfected for 24h with 10 nM of LNA-C, LNA-anti-miR-21 (LNA-21), pre-miR-neg, or pre-miR-21. ( B ) The 786-O cells were co-transfected with J6-PPRE-TK-Luc reporter plasmid and 10 nM of LNA-C or LNA-anti-miR-21 (LNA-21). At 48 h after transfection, luciferase activity was measured. Luciferase activity in the cells co-transfected with J6-PPRE-TK-Luc and LNA-C was set as 1. ( C ) The 786-O cells were co-transfected with J6-PPRE-TK-Luc reporter plasmid and 10 nM of pre-miR-neg or pre-miR-21. At 48 h after transfection, luciferase activity was measured. Luciferase activity in the cells co-transfected with J6-PPRE-TK-Luc and pre-miR-neg was set as 1. ( D ) The 786-O cells were transfected with 10 nM of LNA-C or LNA-anti-miR-21 (LNA-21). At 48 h after transfection, SLC22A5, CPT1, and ACOX1 expressions were determined by RT-qPCR. PPIA was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Article Snippet: The HK-2 human proximal tubule epithelial cell line, ACHN, and 786-O human renal cancer cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Inhibition, Expressing, Western Blot, Transfection, Plasmid Preparation, Luciferase, Activity Assay, Quantitative RT-PCR