human recombinant prongf protein  (Alomone Labs)


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    Alomone Labs human recombinant prongf protein
    Human Recombinant Prongf Protein, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human recombinant prongf protein/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human recombinant prongf protein - by Bioz Stars, 2022-09
    92/100 stars

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    Alomone Labs pro ngf
    Effect of p75NTR stimulation on lipid-associated genes in Huh7 cells. Huh7 cells were stimulated with 100 ng/ml <t>NGF</t> or 20 ng/ml <t>pro-NGF</t> for 48 h, and the expression of lipid-associated genes was analyzed by qPCR. Primers are shown in Table 2 . Results were normalized to GAPDH and controls set to 1. Values are mean ± SD, n = 3-4. *** p
    Pro Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pro ngf/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pro ngf - by Bioz Stars, 2022-09
    93/100 stars
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    91
    Alomone Labs human recombinant prongfs
    Effect of p75NTR stimulation on lipid-associated genes in Huh7 cells. Huh7 cells were stimulated with 100 ng/ml <t>NGF</t> or 20 ng/ml <t>pro-NGF</t> for 48 h, and the expression of lipid-associated genes was analyzed by qPCR. Primers are shown in Table 2 . Results were normalized to GAPDH and controls set to 1. Values are mean ± SD, n = 3-4. *** p
    Human Recombinant Prongfs, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human recombinant prongfs/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human recombinant prongfs - by Bioz Stars, 2022-09
    91/100 stars
      Buy from Supplier

    92
    Alomone Labs human recombinant prongf protein
    Effect of p75NTR stimulation on lipid-associated genes in Huh7 cells. Huh7 cells were stimulated with 100 ng/ml <t>NGF</t> or 20 ng/ml <t>pro-NGF</t> for 48 h, and the expression of lipid-associated genes was analyzed by qPCR. Primers are shown in Table 2 . Results were normalized to GAPDH and controls set to 1. Values are mean ± SD, n = 3-4. *** p
    Human Recombinant Prongf Protein, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human recombinant prongf protein/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human recombinant prongf protein - by Bioz Stars, 2022-09
    92/100 stars
      Buy from Supplier

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    Effect of p75NTR stimulation on lipid-associated genes in Huh7 cells. Huh7 cells were stimulated with 100 ng/ml NGF or 20 ng/ml pro-NGF for 48 h, and the expression of lipid-associated genes was analyzed by qPCR. Primers are shown in Table 2 . Results were normalized to GAPDH and controls set to 1. Values are mean ± SD, n = 3-4. *** p

    Journal: Cell Death & Disease

    Article Title: Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells

    doi: 10.1038/s41419-019-1758-z

    Figure Lengend Snippet: Effect of p75NTR stimulation on lipid-associated genes in Huh7 cells. Huh7 cells were stimulated with 100 ng/ml NGF or 20 ng/ml pro-NGF for 48 h, and the expression of lipid-associated genes was analyzed by qPCR. Primers are shown in Table 2 . Results were normalized to GAPDH and controls set to 1. Values are mean ± SD, n = 3-4. *** p

    Article Snippet: Cells were stimulated with different concentrations of NGF or pro-NGF (cleavage-resistant, mutant protein) (Alamone Labs) for various periods of times.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Schematic view of the role of caspase-2 in p75NTR signaling and lipid metabolism. We propose the following scheme for the action of p75NTR and of caspase-2 in SREBP2 and associated gene regulation. Stimulation of p75NTR by NGF or pro-NGF activates the signaling protein p38 MAPK that can phosphorylate caspase-2 (CASP2) leading to the release of caspase-3 (CASP3) from its complex. CASP3, in turn, can cleave SREBP molecules to yield an active transcription factor. SREBP responsive genes include LDLR and other lipogenic genes but also CASP2 itself. Normally SREBP is processed by the enzymes S1P and S2P via a feedback inhibition determined by the cell cholesterol level. We hypothesize that the steroid-mediated and neurotrophin/p75NTR-induced SREBP pathways are distinct but functionally linked

    Journal: Cell Death & Disease

    Article Title: Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells

    doi: 10.1038/s41419-019-1758-z

    Figure Lengend Snippet: Schematic view of the role of caspase-2 in p75NTR signaling and lipid metabolism. We propose the following scheme for the action of p75NTR and of caspase-2 in SREBP2 and associated gene regulation. Stimulation of p75NTR by NGF or pro-NGF activates the signaling protein p38 MAPK that can phosphorylate caspase-2 (CASP2) leading to the release of caspase-3 (CASP3) from its complex. CASP3, in turn, can cleave SREBP molecules to yield an active transcription factor. SREBP responsive genes include LDLR and other lipogenic genes but also CASP2 itself. Normally SREBP is processed by the enzymes S1P and S2P via a feedback inhibition determined by the cell cholesterol level. We hypothesize that the steroid-mediated and neurotrophin/p75NTR-induced SREBP pathways are distinct but functionally linked

    Article Snippet: Cells were stimulated with different concentrations of NGF or pro-NGF (cleavage-resistant, mutant protein) (Alamone Labs) for various periods of times.

    Techniques: Inhibition

    NGF is expressed in fatty liver of obese mice together with p75NTR. a NGF has increased in leptin-deficient ob/ob mouse livers compared with control, wild-type (wt) mice. b p75NTR was expressed in liver with no significant change between ob/ob and control mice. c , d The level of NGF has increased in leptin-receptor-deficient db/db mouse liver in comparison to controls, c immunoblot analysis and d quantification. ** p

    Journal: Cell Death & Disease

    Article Title: Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells

    doi: 10.1038/s41419-019-1758-z

    Figure Lengend Snippet: NGF is expressed in fatty liver of obese mice together with p75NTR. a NGF has increased in leptin-deficient ob/ob mouse livers compared with control, wild-type (wt) mice. b p75NTR was expressed in liver with no significant change between ob/ob and control mice. c , d The level of NGF has increased in leptin-receptor-deficient db/db mouse liver in comparison to controls, c immunoblot analysis and d quantification. ** p

    Article Snippet: Cells were stimulated with different concentrations of NGF or pro-NGF (cleavage-resistant, mutant protein) (Alamone Labs) for various periods of times.

    Techniques: Mouse Assay

    p75NTR stimulation activated SREBP1 and SREBP2 in Huh7 hepatocyte cells. Human Huh7 cells were stimulated with 10 ng/ml pro-NGF or 50 ng/ml NGF for 16 h followed by immunoblotting using antibodies to detect the presence of cleaved/activated SREBP1 and SREBP2. a SREBP1, immunoblots and b quantification. Values are mean ± SD, n = 3. ** P

    Journal: Cell Death & Disease

    Article Title: Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells

    doi: 10.1038/s41419-019-1758-z

    Figure Lengend Snippet: p75NTR stimulation activated SREBP1 and SREBP2 in Huh7 hepatocyte cells. Human Huh7 cells were stimulated with 10 ng/ml pro-NGF or 50 ng/ml NGF for 16 h followed by immunoblotting using antibodies to detect the presence of cleaved/activated SREBP1 and SREBP2. a SREBP1, immunoblots and b quantification. Values are mean ± SD, n = 3. ** P

    Article Snippet: Cells were stimulated with different concentrations of NGF or pro-NGF (cleavage-resistant, mutant protein) (Alamone Labs) for various periods of times.

    Techniques: Western Blot

    Role of Caspase-2 in p75NTR mediated regulation of SREBP. a Structure of human caspase-2 (CASP2) showing the CARD domain and the 18 and 13 kDa caspase subunits. p38 MAPK phosphorylation site at threonine (Thr)180 in CASP2 is conserved among species. The amino acid numbering underlying functional domains in CASP2 is shown. Boxes represent sequence homology among mammalian species. b Phosphoprotein retardation was performed as described in Materials and methods. A specific phospho-CASP2 antibody against Thr180 was used in conjunction with an antibody against CASP2, recognizing non-phosphorylated form of the enzyme. Phosphorylated CASP2 migrated slower in the Phos-Tag gel (right) as compared with the protein run on denaturating SDS-PAGE (left). c , d Huh7 hepatocyte cells were stimulated with 50 ng NGF ( c ) or 10 ng/ml pro-NGF ( d ) for different times followed by immunoblotting using phospho-CASP2 and anti-CASP2 antibodies. β-Actin was used as a control. e , f Cells were treated with pro-NGF for 6 h in the absence or presence 1 μM of the p38 MAPK inhibitor, SB203580 (SB). e Immunoblot and f quantification of p-CASP2 levels. SB reduced the increase in p-CASP2 by pro-NGF. Values are means ± SD, n = 4. * p

    Journal: Cell Death & Disease

    Article Title: Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells

    doi: 10.1038/s41419-019-1758-z

    Figure Lengend Snippet: Role of Caspase-2 in p75NTR mediated regulation of SREBP. a Structure of human caspase-2 (CASP2) showing the CARD domain and the 18 and 13 kDa caspase subunits. p38 MAPK phosphorylation site at threonine (Thr)180 in CASP2 is conserved among species. The amino acid numbering underlying functional domains in CASP2 is shown. Boxes represent sequence homology among mammalian species. b Phosphoprotein retardation was performed as described in Materials and methods. A specific phospho-CASP2 antibody against Thr180 was used in conjunction with an antibody against CASP2, recognizing non-phosphorylated form of the enzyme. Phosphorylated CASP2 migrated slower in the Phos-Tag gel (right) as compared with the protein run on denaturating SDS-PAGE (left). c , d Huh7 hepatocyte cells were stimulated with 50 ng NGF ( c ) or 10 ng/ml pro-NGF ( d ) for different times followed by immunoblotting using phospho-CASP2 and anti-CASP2 antibodies. β-Actin was used as a control. e , f Cells were treated with pro-NGF for 6 h in the absence or presence 1 μM of the p38 MAPK inhibitor, SB203580 (SB). e Immunoblot and f quantification of p-CASP2 levels. SB reduced the increase in p-CASP2 by pro-NGF. Values are means ± SD, n = 4. * p

    Article Snippet: Cells were stimulated with different concentrations of NGF or pro-NGF (cleavage-resistant, mutant protein) (Alamone Labs) for various periods of times.

    Techniques: Functional Assay, Sequencing, SDS Page