human recombinant nts  (Alomone Labs)


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    Structured Review

    Alomone Labs human recombinant nts
    The survival response of MTNs to <t>NTs</t> varies with time in culture. MTNs were purified from E5.5 chick embryos and cultured in the presence of <t>MEX</t> (300 μg/ml) for 24 hr ( black bars ) or 72 hr ( white bars ), whereupon cultures were rinsed three times
    Human Recombinant Nts, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Images

    1) Product Images from "Development of Survival Responsiveness to Brain-Derived Neurotrophic Factor, Neurotrophin 3 and Neurotrophin 4/5, But Not to Nerve Growth Factor, in Cultured Motoneurons from Chick Embryo Spinal Cord"

    Article Title: Development of Survival Responsiveness to Brain-Derived Neurotrophic Factor, Neurotrophin 3 and Neurotrophin 4/5, But Not to Nerve Growth Factor, in Cultured Motoneurons from Chick Embryo Spinal Cord

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.18-19-07903.1998

    The survival response of MTNs to NTs varies with time in culture. MTNs were purified from E5.5 chick embryos and cultured in the presence of MEX (300 μg/ml) for 24 hr ( black bars ) or 72 hr ( white bars ), whereupon cultures were rinsed three times
    Figure Legend Snippet: The survival response of MTNs to NTs varies with time in culture. MTNs were purified from E5.5 chick embryos and cultured in the presence of MEX (300 μg/ml) for 24 hr ( black bars ) or 72 hr ( white bars ), whereupon cultures were rinsed three times

    Techniques Used: Purification, Cell Culture

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    Alomone Labs nerve growth factor ngf
    <t>BDNF-induced</t> NR2C upregulation by TrkB activation. Cells were cultured in low KCl and treated with or without BDNF (100 ng/ml in A and 50 ng/ml in B-D and F ) for 96 h. A , Levels of indicated mRNAs were quantitated by RNA blotting ( n = 4). B , Cell lysates (40 μg) were immunoblotted with anti-panNR2 antibody or anti-NR1 antibody. Molecular sizes (kilodaltons) of protein makers are indicated on the left. C , P2 membrane fractions were isolated, solubilized, and immunoprecipitated (IP) with anti-NR1 antibody, followed by immnoblotting with anti-panNR2 antibody. D , Cell-surface proteins were biotinylated with Sulfo-NHS-SS-Biotin. Cell lysates were solubilized, precipitated with NeutrAvidin beads, and immunoblotted with anti-panNR2 antibody. E , Cells were cultured in low KCl and treated with BDNF, <t>NGF,</t> NT-3, or NT-4 (50 ng/ml each) for 96 h. Levels of NR2C mRNA were quantitated ( n = 4). F , Granule cells were prepared from TrkB - / - knock-out mice(KO) and their wild-type (WT) littermates. NR2C and TrkB mRNAs were analyzed by RNA blotting. Ethidium bromide-stained 18s rRNA is also indicated. * p
    Nerve Growth Factor Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Alomone Labs anti nt3 antibody
    <t>NT3</t> staining of PV. Perisomatic endings are stained at all ages. Same symbols for cell types as in . Cochlear axons at P30 are heavily stained (thin arrow) along with clusters of nerve terminals at P30 and P8 and the incoming terminals at P14
    Anti Nt3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    BDNF-induced NR2C upregulation by TrkB activation. Cells were cultured in low KCl and treated with or without BDNF (100 ng/ml in A and 50 ng/ml in B-D and F ) for 96 h. A , Levels of indicated mRNAs were quantitated by RNA blotting ( n = 4). B , Cell lysates (40 μg) were immunoblotted with anti-panNR2 antibody or anti-NR1 antibody. Molecular sizes (kilodaltons) of protein makers are indicated on the left. C , P2 membrane fractions were isolated, solubilized, and immunoprecipitated (IP) with anti-NR1 antibody, followed by immnoblotting with anti-panNR2 antibody. D , Cell-surface proteins were biotinylated with Sulfo-NHS-SS-Biotin. Cell lysates were solubilized, precipitated with NeutrAvidin beads, and immunoblotted with anti-panNR2 antibody. E , Cells were cultured in low KCl and treated with BDNF, NGF, NT-3, or NT-4 (50 ng/ml each) for 96 h. Levels of NR2C mRNA were quantitated ( n = 4). F , Granule cells were prepared from TrkB - / - knock-out mice(KO) and their wild-type (WT) littermates. NR2C and TrkB mRNAs were analyzed by RNA blotting. Ethidium bromide-stained 18s rRNA is also indicated. * p

    Journal: The Journal of Neuroscience

    Article Title: Neuronal Depolarization Controls Brain-Derived Neurotrophic Factor-Induced Upregulation of NR2C NMDA Receptor via Calcineurin Signaling

    doi: 10.1523/JNEUROSCI.2191-05.2005

    Figure Lengend Snippet: BDNF-induced NR2C upregulation by TrkB activation. Cells were cultured in low KCl and treated with or without BDNF (100 ng/ml in A and 50 ng/ml in B-D and F ) for 96 h. A , Levels of indicated mRNAs were quantitated by RNA blotting ( n = 4). B , Cell lysates (40 μg) were immunoblotted with anti-panNR2 antibody or anti-NR1 antibody. Molecular sizes (kilodaltons) of protein makers are indicated on the left. C , P2 membrane fractions were isolated, solubilized, and immunoprecipitated (IP) with anti-NR1 antibody, followed by immnoblotting with anti-panNR2 antibody. D , Cell-surface proteins were biotinylated with Sulfo-NHS-SS-Biotin. Cell lysates were solubilized, precipitated with NeutrAvidin beads, and immunoblotted with anti-panNR2 antibody. E , Cells were cultured in low KCl and treated with BDNF, NGF, NT-3, or NT-4 (50 ng/ml each) for 96 h. Levels of NR2C mRNA were quantitated ( n = 4). F , Granule cells were prepared from TrkB - / - knock-out mice(KO) and their wild-type (WT) littermates. NR2C and TrkB mRNAs were analyzed by RNA blotting. Ethidium bromide-stained 18s rRNA is also indicated. * p

    Article Snippet: Slices were treated with or without 1 μ m FK506 for 96 h, and 0.5 vol of the culture medium was changed every 2 d. Reagents and inhibitors were purchased from the following sources: BDNF (Peprotech, London, UK); neurotrophin-3 (NT-3), NT-4, and nerve growth factor (NGF) (Alomone Laboratories, Jerusalem, Israel); neuregulin-β (NeoMarkers, Fremont, CA); nifedipine (Nacalai Tesque, Kyoto, Japan); KN62 (Tocris, Bristol, UK); and U0126, SB203580, FK506, K252a, , PD98059, cyclosporin A, rapamycin, and bisindolylmaleimide I (Calbiochem, San Diego, CA).

    Techniques: Activation Assay, Cell Culture, Isolation, Immunoprecipitation, Knock-Out, Mouse Assay, Staining

    NT3 staining of PV. Perisomatic endings are stained at all ages. Same symbols for cell types as in . Cochlear axons at P30 are heavily stained (thin arrow) along with clusters of nerve terminals at P30 and P8 and the incoming terminals at P14

    Journal: Journal of neuroscience research

    Article Title: Postnatal Development of NT3 and TrkC in Mouse Ventral Cochlear Nucleus

    doi: 10.1002/jnr.22179

    Figure Lengend Snippet: NT3 staining of PV. Perisomatic endings are stained at all ages. Same symbols for cell types as in . Cochlear axons at P30 are heavily stained (thin arrow) along with clusters of nerve terminals at P30 and P8 and the incoming terminals at P14

    Article Snippet: Anti-NT3 antibody was from Alomone Laboratories (Jerusalem, Israel), anti-TrkC was from R & D Systems (Min-neapolis, MN), and anti-SV2 was from the Developmental Studies Hybridoma Bank (Department of Biological Sciences, Iowa City, IA).

    Techniques: Staining

    Fluoresecent double labeling for NT3 (green) and SV2 (red) to show colocalization (yellow) in the PV region (P60). Left arrow, axons abundant with NT3; right arrow, NT3 in the perisomatic region. SV2 labels nerve terminals (double arrows); many of them

    Journal: Journal of neuroscience research

    Article Title: Postnatal Development of NT3 and TrkC in Mouse Ventral Cochlear Nucleus

    doi: 10.1002/jnr.22179

    Figure Lengend Snippet: Fluoresecent double labeling for NT3 (green) and SV2 (red) to show colocalization (yellow) in the PV region (P60). Left arrow, axons abundant with NT3; right arrow, NT3 in the perisomatic region. SV2 labels nerve terminals (double arrows); many of them

    Article Snippet: Anti-NT3 antibody was from Alomone Laboratories (Jerusalem, Israel), anti-TrkC was from R & D Systems (Min-neapolis, MN), and anti-SV2 was from the Developmental Studies Hybridoma Bank (Department of Biological Sciences, Iowa City, IA).

    Techniques: Labeling

    Comparison of perisomatic staining of NT3 and perikaryal location of TrkC in globular bushy cells (thick arrows; P30). Images taken at high magnification (×63 oil). Astrocytes are also stained for TrkC (thin arrows). Inset: A cell with granular

    Journal: Journal of neuroscience research

    Article Title: Postnatal Development of NT3 and TrkC in Mouse Ventral Cochlear Nucleus

    doi: 10.1002/jnr.22179

    Figure Lengend Snippet: Comparison of perisomatic staining of NT3 and perikaryal location of TrkC in globular bushy cells (thick arrows; P30). Images taken at high magnification (×63 oil). Astrocytes are also stained for TrkC (thin arrows). Inset: A cell with granular

    Article Snippet: Anti-NT3 antibody was from Alomone Laboratories (Jerusalem, Israel), anti-TrkC was from R & D Systems (Min-neapolis, MN), and anti-SV2 was from the Developmental Studies Hybridoma Bank (Department of Biological Sciences, Iowa City, IA).

    Techniques: Staining

    Axonal stimulation with growth-promoting and growth-inhibiting stimuli alters axonal mRNA localization. (A and B) The changes in axonal levels of representative mRNAs in response to NGF, BDNF, and NT-3 (A) or MAG-Fc and Sema3A-AP (B) from Table I are graphically illustrated. Positive values indicate an increase in axonal mRNA content, and negative values indicate a decrease in axonal mRNA content detected by qPCR. The axonal mRNA values for neurotrophins are expressed relative to axons treated with BSA. Values for MAG are expressed relative to human IgG Fc, and those for Sema3A are expressed relative to AP (there were no statistically significant differences between the Fc and AP controls; not depicted). Error bars represent the SD of three replicate experiments with each sample measured in quadruplicate. Significance was calculated based on P ≤ 0.01 by a student Newman-Keul test compared with the control. Note that the neurotrophins, MAG, and Sema3A selectively increase or decrease the transport of individual mRNAs. No statistically significant changes were seen in axonal levels of αB crystallin, grp78/BiP, or HCN4 mRNAs with NGF, BDNF, or NT3 treatments. No statistically significant changes were seen in axonal levels of GAP43, Kv3.1a, or peripherin mRNAs with MAG or Sema3A treatments.

    Journal: The Journal of Cell Biology

    Article Title: Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs

    doi: 10.1083/jcb.200703209

    Figure Lengend Snippet: Axonal stimulation with growth-promoting and growth-inhibiting stimuli alters axonal mRNA localization. (A and B) The changes in axonal levels of representative mRNAs in response to NGF, BDNF, and NT-3 (A) or MAG-Fc and Sema3A-AP (B) from Table I are graphically illustrated. Positive values indicate an increase in axonal mRNA content, and negative values indicate a decrease in axonal mRNA content detected by qPCR. The axonal mRNA values for neurotrophins are expressed relative to axons treated with BSA. Values for MAG are expressed relative to human IgG Fc, and those for Sema3A are expressed relative to AP (there were no statistically significant differences between the Fc and AP controls; not depicted). Error bars represent the SD of three replicate experiments with each sample measured in quadruplicate. Significance was calculated based on P ≤ 0.01 by a student Newman-Keul test compared with the control. Note that the neurotrophins, MAG, and Sema3A selectively increase or decrease the transport of individual mRNAs. No statistically significant changes were seen in axonal levels of αB crystallin, grp78/BiP, or HCN4 mRNAs with NGF, BDNF, or NT3 treatments. No statistically significant changes were seen in axonal levels of GAP43, Kv3.1a, or peripherin mRNAs with MAG or Sema3A treatments.

    Article Snippet: Pharmacological reagentsNGF (Harlan), BDNF, NT3 (Alomone Labs), MAG-Fc (R & D Systems), and Sema3A-AP ( ) were covalently coupled to 15-μm-diameter polystyrene microparticles according to the manufacturer's instructions (Polysciences).

    Techniques: Real-time Polymerase Chain Reaction

    BDNF modulation required tropomyosin-related kinase B (trkB) receptors and NMDA receptors.  A : effect of BDNF with either bath-applied K-252a (200 nM;  n  = 4) or intracellular K-252a (200 nM;  n  = 5) or bath-applied neurotrophin 3 (NT-3) alone (20 ng/ml;  n  = 7).  B : lack of effect of BDNF on mEPSC decay time in the presence of CPP (5 μM), 2 mM extracellular Mg 2+ , or intracellular MK-801 (500 μM). Under these conditions, decay time was fit to a single exponential. In this and all subsequent figures, numbers in parentheses indicate number of experiments.  C : lack of effect of BDNF on mEPSC frequency in the presence of CPP ( n  = 6). All recordings were from layer 5 PNs. * P

    Journal: Journal of Neurophysiology

    Article Title: Presynaptic and Postsynaptic NMDA Receptors Mediate Distinct Effects of Brain-Derived Neurotrophic Factor on Synaptic Transmission

    doi: 10.1152/jn.90880.2008

    Figure Lengend Snippet: BDNF modulation required tropomyosin-related kinase B (trkB) receptors and NMDA receptors. A : effect of BDNF with either bath-applied K-252a (200 nM; n = 4) or intracellular K-252a (200 nM; n = 5) or bath-applied neurotrophin 3 (NT-3) alone (20 ng/ml; n = 7). B : lack of effect of BDNF on mEPSC decay time in the presence of CPP (5 μM), 2 mM extracellular Mg 2+ , or intracellular MK-801 (500 μM). Under these conditions, decay time was fit to a single exponential. In this and all subsequent figures, numbers in parentheses indicate number of experiments. C : lack of effect of BDNF on mEPSC frequency in the presence of CPP ( n = 6). All recordings were from layer 5 PNs. * P

    Article Snippet: BDNF, neurotrophin-3 (NT-3), and TTX were obtained from Alomone Labs (Jerusalem, Israel).

    Techniques: Conditioned Place Preference