Human Recombinant Egf, supplied by PeproTech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice"
Article Title: Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice
Journal: Development (Cambridge, England)
Figure Legend Snippet: GDF15 promotes the response to EGF and EGFR expression in hippocampal precursors. (A-D) Quantitative analysis of the total cell number (A,C,D) and clones (B) obtained upon plating E18 (A-C) and E14 (D) wild-type and Gdf15 -/- cells in both EGF (E) and FGF2 (F) (A,B,D), or E and/or F, as indicated (C). In A, exogenous GDF15 was added as shown. In C, values are the percentage variation of the total cell number scored in parallel wild-type cultures grown in both growth factors. (E,F) Representative dot plots of dissociated E18 wild-type (E) and Gdf15 -/- (F) HP after staining with EGF-Alexa 488 and PI to reveal EGFR low (R1), EGFR high (R2) and dead cells (R3). Values represent the means±s.e.m. of the percentage of total cells in each gate. (G,H) Quantitative analysis of the EGFR high cell numbers in the E18 wild-type and Gdf15 -/- HP measured at day in vitro (DIV) 0, or after 24 hours exposure to FGF2 (DIV 1) (G) and of the percentage of E18 wild-type and Gdf15 -/- EGFR high cells capable of undergoing clone formation upon plating at DIV0 (H). * and # indicate values that are significantly different from the corresponding wild-type samples and the counterpart at DIV0, respectively. * P ≤0.05; ** P ≤0.01; ***,### P ≤0.001; n ≥3.
Techniques Used: Expressing, Staining, In Vitro
2) Product Images from "Tanshinone IIA reverses EGF- and TGF-β1-mediated epithelial-mesenchymal transition in HepG2 cells via the PI3K/Akt/ERK signaling pathway"
Article Title: Tanshinone IIA reverses EGF- and TGF-β1-mediated epithelial-mesenchymal transition in HepG2 cells via the PI3K/Akt/ERK signaling pathway
Journal: Oncology Letters
Figure Legend Snippet: Effects of Tan IIA on the viability and clonogenic potential of HepG2 cells. (A) Chemical structure of Tan IIA. (B) Cell viability analysis of HepG2 cells cultured in the presence of Tan IIA for 24, 48 and 72 h, by MTT assay. (C) Colony formation assay of HepG2 cells that were untreated or treated with 20 ng/ml EGF, 10 ng/ml TGF-β1 and Tan IIA (0.5, 1 and 2 µM) for 2 weeks. *P
Techniques Used: Cell Culture, MTT Assay, Colony Assay
Figure Legend Snippet: Effects of Tan IIA on EGF- and TGF-β1-induced migration and invasion of HepG2 cells. The migration and invasion of HepG2 cells that were untreated or treated with (A) 20 ng/ml EGF, (B) 10 ng/ml TGF-β1 and Tan IIA (0.5, 1 and 2 µM) were analyzed by wound healing and Transwell assays, respectively. Scale bars, 100 µm. **P
Techniques Used: Migration
Figure Legend Snippet: Tan IIA inhibits EMT by deactivating the PI3K/Akt/ERK signaling pathway in EGF- and TGF-β1-treated HepG2 cells. (A) Phosphorylation and expression of Akt and ERK1/2 in HepG2 cells that were untreated or treated with 20 ng/ml EGF, 10 ng/ml TGF-β1 and 2 µM Tan IIA were analyzed by western blotting. (B) Protein expression levels of E-cadherin, vimentin and Snail in HepG2 cells that were untreated or treated with 20 ng/ml EGF, 10 ng/ml TGF-β1, 20 µM LY, and 20 µM U0126 were analyzed by western blotting. **P
Techniques Used: Expressing, Western Blot
Figure Legend Snippet: Tan IIA reverses EGF- and TGF-β1-induced EMT in HepG2 cells. (A) E-cadherin and vimentin expression in HepG2 cells was determined by confocal microscopy. Scale bars, 20 µm. (B) Expression of E-cadherin, MMP-2, N-cadherin, vimentin and Snail in HepG2 cells that were untreated or treated with 20 ng/ml EGF, 10 ng/ml TGF-β1 and 2 µM Tan IIA were analyzed via western blotting. **P
Techniques Used: Expressing, Confocal Microscopy, Western Blot
3) Product Images from "Bio-Imaging of Colorectal Cancer Models Using Near Infrared Labeled Epidermal Growth Factor"
Article Title: Bio-Imaging of Colorectal Cancer Models Using Near Infrared Labeled Epidermal Growth Factor
Journal: PLoS ONE
Figure Legend Snippet: Synthesis, purification, spectrum, electrophoresis properties and signaling of EGF-NIR. (A) Reaction scheme for the synthesis of EGF-NIR conjugate, the first amino acid asparagine at the amino terminal is indicated as Asn1. (B) Separation of synthesis reaction mixture on gel permeation chromatography and of EGF-NIR sample from gel permeation on (C) anion exchange chromatography; EGF-NIR-full line (800 nm); gradient of NaCl-broken line; unconjugated EGF-dotted line. (D) HPLC separation of EGF-NIR purified from anion exchanger chromatography. Full line represents absorbance at 226 nm and dotted line indicates the gradient. Insert-12% SDS-PAGE analysis of 10 µg of EGF-NIR scanned with Odyssey and unmodified EGF stained with coomassie blue. (E) NIR spectrum of EGF-NIR [excitation (gray line) and emission (black line)]; Insert-IRDye 800CW NHS ester; (F) EGF-NIR induced Erk phosphorylation.
Techniques Used: Purification, Electrophoresis, GPC Assay, Chromatography, High Performance Liquid Chromatography, SDS Page, Staining
Article Title: Probing the Heterogeneity of Protein Kinase Activation in Cells by Super-resolution Microscopy
Article Snippet: On the next day, cells were washed twice with serum-free growth medium before they were incubated with the anti-EGFR antibody directed against the extracellular EGFR domain (clone 108) that was conjugated to colloidal 10 nm gold particles as previously described. .. Optimum gold loading into the cells was observed for 30 min of incubation with 80 nM
Article Title: Human pancreatic cancer stem cells are sensitive to dual inhibition of IGF-IR and ErbB receptors
Article Snippet: Insulin-like growth factor (IGF-I) and
Article Title: High Yield of Adult Oligodendrocyte Lineage Cells Obtained from Meningeal Biopsy
Article Snippet: NS Medium Neurobasal medium (Thermo Fisher Scientific), 2% B27 supplement (Thermo Fisher Scientific), 1% N2 supplement (Thermo Fisher Scientific), 2 mM glutamine (Thermo Fisher Scientific), 100 U/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific), 20 ng/ml
Article Title: miR-29a contributes to breast cancer cells epithelial–mesenchymal transition, migration, and invasion via down-regulating histone H4K20 trimethylation through directly targeting SUV420H2
Article Snippet: 3D semi-solid spheres culture Three thousand single cells were seeded into 24-well Ultra-Low Attachment Microplates (Corning) in serum-free DMEM/F12 (Invitrogen), supplemented with B27 (1:50, Invitrogen), 20 ng/ml
Article Title: Investigation of brain tissue infiltration by medulloblastoma cells in an ex vivo model
Article Snippet: Reagents Antibodies: goat anti-GFAP (ab53554, 1:300), rabbit anti-Calbindin (ab11426, 1:1000), mouse anti-Calbindin (ab82812, 1:1000), donkey anti-rabbit (ab175649, 1: 100) all from Abcam and donkey anti-goat (A11057, 1:500), Vybrant CM-DiI cell labelling solution (V-2288) from Life Technologies, mouse anti-NeuN clone A60 (MAB377, 1 in 100), mouse anti-GFAP (MAB360, 1:500), rabbit anti-collagen type IV (AB756P, 1:500), anti-nuclei clone 3E1.3(MAB4383) from Millipore, rabbit anti-cleaved caspase 3 (5A1E, 1:500) from Cell Signaling Technology, Click-iT EdU Alexa Fluor-647 imaging kit from Molecular Probes. .. HGF and