human placenta rnase inhibitor  (New England Biolabs)


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  • 99
    Name:
    RNase I
    Description:
    RNase I 25 000 units
    Catalog Number:
    m0243l
    Price:
    270
    Size:
    25 000 units
    Category:
    Ribonucleases RNase
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    Structured Review

    New England Biolabs human placenta rnase inhibitor
    RNase I
    RNase I 25 000 units
    https://www.bioz.com/result/human placenta rnase inhibitor/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human placenta rnase inhibitor - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: RNase If -treated quantitative PCR for dsRNA quantitation of RNAi trait in genetically modified crops
    Article Snippet: .. RNase If t reatment The isolated RNA was digested with RNase If (New England Biolabs, Ipswich, MA, USA) per the manufacturer’s protocol at 37 °C for 10 min followed by heat inactivation at 70 °C for 20 min. .. The digested samples were purified using the RNA Clean-up and concentration kit (Norgen) following the manufacturer’s protocol.

    Labeling:

    Article Title: Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes
    Article Snippet: .. Surprisingly, fragmentation with RNase III dramatically increased the labeling efficiency of cRNA to > 90% (Figure ). .. We also fragmented cRNA labeled internally during IVT and tested for the level of biotin incorporation using a gel shift assay (Figure ).

    Purification:

    Article Title: RNA targeting with CRISPR-Cas13a
    Article Snippet: .. Briefly, reactions consisted of 45 nM purified LwaCas13a, 22.5 nM crRNA, 125 nM quenched fluorescent RNA reporter (RNAse Alert v2, Thermo Scientific), 2 μL murine RNase inhibitor (New England Biolabs), 100 ng of background total human RNA (purified from HEK293FT culture), and varying amounts of input nucleic acid target, unless otherwise indicated, in nuclease assay buffer (40 mM Tris-HCl, 60 mM NaCl, 6 mM MgCl2, pH 7.3). .. Reactions were allowed to proceed for 1-3 hr at 37°C (unless otherwise indicated) on a fluorescent plate reader (BioTek) with fluorescent kinetics measured every 5 min.

    Produced:

    Article Title: Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes
    Article Snippet: .. Both enzymes fragmented cRNA, however RNase III produced a fragment size range more similar to the standard method of magnesium hydrolysis (Figure ). cRNA, total RNA and poly(A) RNA were all fragmented by RNase III and the average fragment size ranged from ∼20 to 200 nt. .. A benefit of RNase fragmentation is that dephosphorylation can be performed simultaneously using SAP.

    Incubation:

    Article Title: An origin of the immunogenicity of in vitro transcribed RNA
    Article Snippet: .. 100 ng/μl of RNA was incubated with indicated concentrations of RNase III (NEB) or RNase If (NEB) in 10 mM Tris, pH 8.5 and 2 mM MgCl2 at 37°C for 30 min. .. The reaction was terminated using 1/10 volume of proteinase K (NEB) and incubated at 25°C for 20 min. RNA was purified with Direct-zol RNA MiniPrep Kit (Zymo research) followed by QIAquick PCR purification kit (Qiagen).

    other:

    Article Title: Recognition and discrimination of target mRNAs by Sib RNAs, a cis-encoded sRNA family
    Article Snippet: These results suggest that the SibC–ibsC mRNA complex with unpaired regions is a poorer substrate for RNase III than the complete duplex.

    Article Title: Cellular 5′-3′ mRNA Exonuclease Xrn1 Controls Double-stranded RNA Accumulation and Anti-Viral Responses
    Article Snippet: Beads were then washed with IP buffer 4 times then treated with either 20u/ml RNase III or 20 μl/ml RNaseA/T1 (25u/ml /1000u/ml respectively) for 15 min at 37°C.

    Nuclease Assay:

    Article Title: RNA targeting with CRISPR-Cas13a
    Article Snippet: .. Briefly, reactions consisted of 45 nM purified LwaCas13a, 22.5 nM crRNA, 125 nM quenched fluorescent RNA reporter (RNAse Alert v2, Thermo Scientific), 2 μL murine RNase inhibitor (New England Biolabs), 100 ng of background total human RNA (purified from HEK293FT culture), and varying amounts of input nucleic acid target, unless otherwise indicated, in nuclease assay buffer (40 mM Tris-HCl, 60 mM NaCl, 6 mM MgCl2, pH 7.3). .. Reactions were allowed to proceed for 1-3 hr at 37°C (unless otherwise indicated) on a fluorescent plate reader (BioTek) with fluorescent kinetics measured every 5 min.

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  • 98
    New England Biolabs rnase inhibitor human placenta
    One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the <t>RNase</t> inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) <t>RT-qPCR</t> detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.
    Rnase Inhibitor Human Placenta, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase inhibitor human placenta/product/New England Biolabs
    Average 98 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rnase inhibitor human placenta - by Bioz Stars, 2020-07
    98/100 stars
      Buy from Supplier

    99
    New England Biolabs human placental rnase inhibitor
    <t>RNase</t> activity of <t>YoeB</t> Sa 1 mutants
    Human Placental Rnase Inhibitor, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human placental rnase inhibitor/product/New England Biolabs
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    human placental rnase inhibitor - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the RNase inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) RT-qPCR detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.

    Journal: bioRxiv

    Article Title: Comparison of SARS-CoV-2 Indirect and Direct Detection Methods

    doi: 10.1101/2020.05.12.092387

    Figure Lengend Snippet: One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the RNase inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) RT-qPCR detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.

    Article Snippet: With direct RT-qPCR we found that simply adding RNase inhibitor greatly improved sensitivity, without need for any other treatments (e.g. lysis buffers or boiling).

    Techniques: RNA Extraction, Lysis, Quantitative RT-PCR, SYBR Green Assay

    RNase activity of YoeB Sa 1 mutants

    Journal: Biochemistry

    Article Title: Light Activation of Staphylococcus aureus Toxin YoeBSa1 Reveals Guanosine-Specific Endoribonuclease Activity

    doi: 10.1021/bi4008098

    Figure Lengend Snippet: RNase activity of YoeB Sa 1 mutants

    Article Snippet: For the time course of YoeBSa 1 and YefMSa 1 RNase activity, 10 pmol YoeBSa 1 -Y88ONBY, YoeBSa 1 -Y88F, or YoeBSa 1 -Y88TAG, or YefMSa 1 was incubated with 320 ng yefM-yoeB Sa 1 RNA in the presence of 1 unit/μl Human Placental RNase inhibitor in 50 mM Tris, 5% glycerol, pH 8.0.

    Techniques: Activity Assay