Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Schematic representation of T/C at rs3749117 and G/C at rs35771982 polymorphisms located in the C-type lectin-like domains (CTLD) 1 of PLA 2 R gene

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques:

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Primers used for point mutagenesis of T/C at rs3749117 and G/C at rs35771982 of PLA 2 R gene

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques: Mutagenesis

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Wild-type and mutant human PLA 2 R protein expression in transfected HEK293 cells (A) Western blot analysis of wild-type and mutant PLA 2 R expressed in HEK293 cells using polyclonal antibody for against CTLD5–6 of human PLA 2 R. The apparent molecular weights of wild-type and mutant PLA 2 R are consistent with their calculated molecular weights, indicating efficient translation. No protein band was detected in control mock-transfected cells (mock). (B) Quantitative analysis of the immunoblotting. Protein expression levels of wild-type and mutant PLA 2 R were similar in HEK293 cells transfected with the respective genotypes. (C) Flow cytometric analyses of wild-type and mutant PLA 2 R cell surface expression in HEK293 cells transfected with a cDNA construct encoding wild-type and mutant PLA 2 R using polyclonal antibody against CTLD5–6 of human PLA 2 R. Upper panels, representative dot plots of suspensions of HEK293 cells transfected with wild-type or mutant PLA 2 R; lower panels, representative histograms studying PLA 2 R expression on cells transfected with the respective PLA 2 R genotypes. (D) Comparison of mean fluorescence intensity (MFI) for PLA 2 R expression in HEK293 cells transfected with wild-type or mutant PLA 2 R. (E) PLA 2 R expression in cultures of human umbilical vein endothelial cells (HUVECs), human vascular smooth muscle cells (SMCs), and mock. The mRNA expression levels were quantified with a real-time two-step reverse transcriptase PCR assay. n = 7 in each experiment. NS, statistically not significant.

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques: Mutagenesis, Expressing, Transfection, Western Blot, Construct, Fluorescence

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Comparison of migration, proliferation, and binding to human collagen I between HEK293 cells expressing wild-type PLA 2 R and double mutant PLA 2 R (C at rs3749117 and C at rs35771982) (A) Migration of HEK293 cells expressing wild-type or double mutant type of PLA 2 R on filter membranes coated with PBS (as a control) or collagen I (Coll) in Boyden chamber experiments. (B) Proliferation (measured by light absorbance at 370/492 nm) of cells expressing wild-type or double mutant type of PLA 2 R on dishes coated with PBS or collagen I (Coll). (C) Binding activity to collagen I in cells expressing wild-type or double mutant type of PLA 2 R. Cells were incubated with 125 I-labeled collagen I at 4°C in the absence or presence of a 50-fold excess amount of unlabeled collagen I and washed with cold PBS. The radioactivity of collagen I bound to cells (counts per minute in 100 µ g of protein) is shown after correcting for nonspecific binding. * p < 0.05 in comparison with mock; † p < 0.05 between wild-type; and double mutant type of PLA 2 R. # < 0.05 in comparison with respective genotypes of cells on PBS-coated dishes. n = 6 for each experiment.

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques: Migration, Binding Assay, Expressing, Mutagenesis, Activity Assay, Incubation, Labeling, Radioactivity

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Comparison of migration, proliferation, and binding to human collagen I between HEK293 cells expressing wild-type PLA 2 R and single mutant PLA 2 R (either C at rs3749117 [Singl Mut1] or C at rs35771982 [Singl Mut2]) (A) Migration of HEK293 cells expressing wild-type or single mutant type of PLA 2 R. (B) Proliferation of cells expressing wild-type or single mutant type of PLA 2 R. (C) Binding activity to collagen I in cells expressing wild-type or single mutant type of PLA 2 R. * p < 0.05 in comparison with mock; † p < 0.05 between wild type; and single mutant type of PLA 2 R. # <0.05 in comparison with respective genotypes of cells on PBS-coated dishes. n = 6 for each experiment.

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques: Migration, Binding Assay, Expressing, Mutagenesis, Activity Assay

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Relationships between T/C genotypes at rs3749117 of PLA 2 R gene and an increased maxIMT in male patients

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques:

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Relationships between T/C genotypes at rs3749117 of PLA 2 R gene and an increased maxIMT of the carotid artery in total patient population

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques:

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Relationships between T/C genotypes at rs3749117 of PLA 2 R gene and an increased baPWV (≥ 19 m/sec) in total patient population

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques:

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Relationships between T/C genotypes at rs3749117 of PLA 2 R gene and a decreased ABI ( <0.88) in female patients

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques:

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Univariate and multivariate logistic regression analysis of association of C/C genotype at rs3749117 of PLA 2 R gene and cardiovascular risk factors with an increased maxIMT of the carotid artery in male patients

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques:

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Relationships between T/C genotypes at rs3749117 of PLA 2 R gene and an increased maxIMT in female patients

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques:

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Relationships between T/C genotypes at rs3749117 of PLA 2 R gene and a decreased ABI ( <0.88) in total patient population

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques:

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Relationships between T/C genotypes at rs3749117 of PLA 2 R gene and an increased baPWV (≥ 19 m/sec) in male patients

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques:

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Relationships between T/C genotypes at rs3749117 of PLA 2 R gene and a decreased ABI ( <0.88) in male patients

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques:

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: Phospholipase A 2 Receptor Gene Polymorphisms Alter its Functions and Present a Genetic Risk of an Increased Intima-Media Thickness of the Carotid Artery

doi: 10.5551/jat.34330

Figure Lengend Snippet: Relationships between T/C genotypes at rs3749117 of PLA 2 R gene and an increased baPWV ( <19.0 m/sec) in female patients

Article Snippet: Human PLA 2 R wild-type plasmid (NM_001195641) was purchased from Origene (Rockville, MD, USA).

Techniques: