Human Phosphorylated P38 Map Kinase Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Signaling pathways involved in LPS induced TNFalpha production in human adipocytes"
Article Title: Signaling pathways involved in LPS induced TNFalpha production in human adipocytes
Journal: Journal of Inflammation (London, England)
Figure Legend Snippet: Activation of p38 MAP Kinase and NFkappaB pathways regulate LPS induced TNFalpha synthesis from human mature adipocytes . Panel A: Effect of NFkappaB and p38 inhibitors on TNFalpha secretion . The concentrations of TNFalpha (pg/mL) were determined at 6 hours in the medium of mature adipocyte cultures treated or not with: 1 μg/mL of LPS alone; LPS and increasing concentrations of NFkappaB inhibitor (NFkBi 0.1, 0.5 and 1 μM); LPS and increasing concentrations of p38 MAP kinase inhibitor (SB, 0.1, 0.5, 1 and 5 μM); LPS and NFkBi (1 μM) + SB (1 μM). The graph represents the mean ± SE of the results from one patient (n = 6 for each condition), representative of three experiments on three different patients. ***P < 0.001%, *P < 0.05%, versus cells treated with LPS alone. Panel B: TNFalpha gene expression . TNFalpha gene expression was determined at 4, 5 and 6 hours of treatment in mature adipocyte cultures, treated or not with: 1 μg/mL of LPS alone; LPS and NFkappaB inhibitor (NFkBi, 1 μM); LPS and p38 MAP kinase inhibitor (SB, 1 μM); LPS and NFkBi (1 μM) + SB (1 μM). The graph represents the mean ± SD of the results from one patient (n = 5 for each condition), representative of two experiments on two different patients. ***P < 0.001%, *P < 0.05%, versus cells treated with LPS alone.
Techniques Used: Activation Assay, Expressing
Figure Legend Snippet: p38 MAP Kinase phosphorylation by LPS . Mature adipocyte cells were treated with LPS at 1 μg/mL for 5, 10 and 20 min, or with LPS + p38 MAP Kinase inhibitor (SB, 1 μM) for 5 min. Proteins (50 μg per lane) were separated by SDS-PAGE and analyzed by Western blotting using an anti-phospho-p38 MAP Kinase protein antibody (Thr180/Tyr182, panel B ). Loading equality was controlled using antibody against the unphosphorylated isoform of p38 ( panel A ). The data represent a typical result from two independent experiments.
Techniques Used: SDS Page, Western Blot
Figure Legend Snippet: Signaling pathways involved in LPS induced TNFalpha production in human adipocytes . LPS, with LBP and CD14 (coming from medium) bind to TLR4 on mature human adipose cells. Binding activates two main pathways in the cells. One pathway leads to the activation of NFkappaB (through TRAF6 and IKK). The other pathway passes through phosphorylated p38 MAP Kinase. Both pathways enable the activation of TNFalpha transcription, followed by cleavage of the protein via a membrane metalloprotease, ADAM17 or TACE, leading to the release of the soluble form of TNFalpha. The PI3K represents a third pathway, which activates NFkappaB and p38 MAPK. Another kinase, maybe the PI4K, plays an inhibitory role in the LPS activation of these 2 pathways. As far as the PKC is concerned, it probably activates IKK, or the p38 MAPKs, or both pathways. However, activation is only visible once the PI4K is inactive. It is therefore possible that PI4K constitutively inhibits PKC.
Techniques Used: Binding Assay, Activation Assay