human peripheral blood mononucleated cells  (Millipore)


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  • 99
    Name:
    Human Peripheral Blood T Cells
    Description:
    Human Peripheral Blood T Cells HPBT provide a useful in vitro tool for studying the biology and pathology of CD4 cells HPBT cells play critical roles in the regulation of immune responses and are responsible for mediating many of the effector mechanisms of the immune system Increased activity in the field of immunotherapy coupled with the expense of clinical trials has led to renewed interest in obtaining high purity human T cells to meet needs for research and development Our CD4 T Cells are isolated from human blood of healthy donors and cryopreserved immediately after isolation
    Catalog Number:
    6902-50a
    Price:
    None
    Applications:
    Pathology, cancer, autoimmune disorders, infectious diseases. Immune response regulation, direct destruction of infected cells, immunocompetence, scanning of intracellular environment, effector mechanisms, realistic in vitro model, immunotherapeutics. T cell origination, development, differentiation, expansion, proliferation, maturation and activation. Hematopoesis, stem cells, positive & negative selection. T cell receptors (TCRs), antigen recognition, costimulatory molecules, interaction with foreign antigens, signaling cascades and cytokine production.
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    Structured Review

    Millipore human peripheral blood mononucleated cells
    Human Peripheral Blood T Cells
    Human Peripheral Blood T Cells HPBT provide a useful in vitro tool for studying the biology and pathology of CD4 cells HPBT cells play critical roles in the regulation of immune responses and are responsible for mediating many of the effector mechanisms of the immune system Increased activity in the field of immunotherapy coupled with the expense of clinical trials has led to renewed interest in obtaining high purity human T cells to meet needs for research and development Our CD4 T Cells are isolated from human blood of healthy donors and cryopreserved immediately after isolation
    https://www.bioz.com/result/human peripheral blood mononucleated cells/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human peripheral blood mononucleated cells - by Bioz Stars, 2020-09
    99/100 stars

    Related Products / Commonly Used Together

    phytohemagglutinin pha
    ficoll density gradient separation

    Images

    1) Product Images from "Regression of established renal cell carcinoma in nude mice using lentivirus-transduced human T cells expressing a human anti-CAIX chimeric antigen receptor"

    Article Title: Regression of established renal cell carcinoma in nude mice using lentivirus-transduced human T cells expressing a human anti-CAIX chimeric antigen receptor

    Journal: Molecular Therapy Oncolytics

    doi: 10.1038/mto.2014.3

    Antibody-dependent cellular cytotoxicity of carbonic anhydrase IX (CAIX)-specific Antibodies. 1 µg/ml CAIX-specific scFv-Fc minibodies were added to the target tumor cells in the presence of human peripheral blood mononucleated cell (E:T 25:1). Similar results were obtained in two experiments. Irrelevant anti-SARS scFv-Fc (11A) and anti-CCR4 scFv-Fc (48) minibodies were used as negative controls. ( a ) CAIX + sk-rc-09 cells; ( b ) CAIX + sk-rc-52 cells; ( c ) CAIX- sk-rc-59 cells.
    Figure Legend Snippet: Antibody-dependent cellular cytotoxicity of carbonic anhydrase IX (CAIX)-specific Antibodies. 1 µg/ml CAIX-specific scFv-Fc minibodies were added to the target tumor cells in the presence of human peripheral blood mononucleated cell (E:T 25:1). Similar results were obtained in two experiments. Irrelevant anti-SARS scFv-Fc (11A) and anti-CCR4 scFv-Fc (48) minibodies were used as negative controls. ( a ) CAIX + sk-rc-09 cells; ( b ) CAIX + sk-rc-52 cells; ( c ) CAIX- sk-rc-59 cells.

    Techniques Used:

    Related Articles

    Flow Cytometry:

    Article Title: CXCR2-modified CAR-T cells have enhanced trafficking ability that improves treatment of hepatocellular carcinoma.
    Article Snippet: .. Unlike hematological malignancies, solid tumors have proved to be less susceptible to chimeric antigen receptor (CAR)-T cell therapy, which is partially caused by reduced accumulation of therapeutic T cells in tumor site. .. Unlike hematological malignancies, solid tumors have proved to be less susceptible to chimeric antigen receptor (CAR)-T cell therapy, which is partially caused by reduced accumulation of therapeutic T cells in tumor site.

    Sedimentation:

    Article Title: Immune synapse formation requires ZAP-70 recruitment by ezrin and CD43 removal by moesin
    Article Snippet: .. Isolation of primary human T cells Human peripheral blood lymphocytes were isolated from citrate-anticoagulated whole blood by dextran sedimentation (Blood Centers of America) followed by density separation over Ficoll-Hypaque (Sigma-Aldrich). .. The resulting mononuclear cells were washed in PBS and further purified by nylon wool and plastic adherence as described previously ( ).

    Isolation:

    Article Title: Oxidized phospholipids as biomarkers of tissue and cell damage with a focus on cardiolipin
    Article Snippet: .. Human peripheral blood lymphocytes were isolated from whole blood with the use of Histopaque (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer's instructions. ..

    Article Title: Collagen Density Modulates the Immunosuppressive Functions of Tumor-Associated Macrophages
    Article Snippet: .. T cell proliferation For co-culture assays, splenocytes were isolated from BALB/c mice as previously described. .. RAW 264.7 macrophages were cultured in 3D collagen matrices of 1 mg/ml or 4 mg/ml collagen for one day prior to co-culture.

    Article Title: Immune synapse formation requires ZAP-70 recruitment by ezrin and CD43 removal by moesin
    Article Snippet: .. Isolation of primary human T cells Human peripheral blood lymphocytes were isolated from citrate-anticoagulated whole blood by dextran sedimentation (Blood Centers of America) followed by density separation over Ficoll-Hypaque (Sigma-Aldrich). .. The resulting mononuclear cells were washed in PBS and further purified by nylon wool and plastic adherence as described previously ( ).

    Article Title: Cis-trimethoxy resveratrol induces intrinsic apoptosis via prometaphase arrest and prolonged CDK1 activation pathway in human Jurkat T cells
    Article Snippet: .. Isolation and activation of human peripheral T cells To prepare human peripheral blood mononuclear cells (PBMC), heparinized blood obtained from healthy laboratory personnel by venipuncture was centrifuged at 800×g for 20 min over Ficoll (Sigma Chemical, St. Louis, MO, USA), according to the manufacturer's instructions. ..

    Article Title: IL-27 attenuates airway inflammation in a mouse asthma model via the STAT1 and GADD45γ/p38 MAPK pathways
    Article Snippet: .. CD4+ T cell culture and IL-4 protein measurement For the human CD4+ T cells, peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Hypaque (Histopaque-1077; Sigma-Aldrich, St Louis, MO, USA) gradient centrifugation from the 15 mL of peripheral blood collected from each healthy volunteer. .. The CD4+ T cells were isolated from the PBMCs by means of magnetic bead separation (magnetic cell sorting) with a human CD4+ T Cell Isolation Kit (Miltenyi Biotec).

    Co-Culture Assay:

    Article Title: Collagen Density Modulates the Immunosuppressive Functions of Tumor-Associated Macrophages
    Article Snippet: .. T cell proliferation For co-culture assays, splenocytes were isolated from BALB/c mice as previously described. .. RAW 264.7 macrophages were cultured in 3D collagen matrices of 1 mg/ml or 4 mg/ml collagen for one day prior to co-culture.

    Mouse Assay:

    Article Title: Collagen Density Modulates the Immunosuppressive Functions of Tumor-Associated Macrophages
    Article Snippet: .. T cell proliferation For co-culture assays, splenocytes were isolated from BALB/c mice as previously described. .. RAW 264.7 macrophages were cultured in 3D collagen matrices of 1 mg/ml or 4 mg/ml collagen for one day prior to co-culture.

    Activation Assay:

    Article Title: Cis-trimethoxy resveratrol induces intrinsic apoptosis via prometaphase arrest and prolonged CDK1 activation pathway in human Jurkat T cells
    Article Snippet: .. Isolation and activation of human peripheral T cells To prepare human peripheral blood mononuclear cells (PBMC), heparinized blood obtained from healthy laboratory personnel by venipuncture was centrifuged at 800×g for 20 min over Ficoll (Sigma Chemical, St. Louis, MO, USA), according to the manufacturer's instructions. ..

    other:

    Article Title: The innate sensor ZBP1-IRF3 axis regulates cell proliferation in multiple myeloma
    Article Snippet: Human bone marrow and peripheral blood cells from myeloma patients or healthy donor were collected under written consent and research ethical committee approval (REC reference number: 11/H0308/9).

    Article Title: IDH2 and NPM1 Mutations Cooperate to Activate Hoxa9/Meis1 and Hypoxia Pathways in Acute Myeloid Leukemia.
    Article Snippet: The volume of plasma and peripheral blood cells was adjusted to 100 mL prior to the addition of 400 mL of ethanol.

    Cell Culture:

    Article Title: IL-27 attenuates airway inflammation in a mouse asthma model via the STAT1 and GADD45γ/p38 MAPK pathways
    Article Snippet: .. CD4+ T cell culture and IL-4 protein measurement For the human CD4+ T cells, peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Hypaque (Histopaque-1077; Sigma-Aldrich, St Louis, MO, USA) gradient centrifugation from the 15 mL of peripheral blood collected from each healthy volunteer. .. The CD4+ T cells were isolated from the PBMCs by means of magnetic bead separation (magnetic cell sorting) with a human CD4+ T Cell Isolation Kit (Miltenyi Biotec).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: CXCR2-modified CAR-T cells have enhanced trafficking ability that improves treatment of hepatocellular carcinoma.
    Article Snippet: .. Unlike hematological malignancies, solid tumors have proved to be less susceptible to chimeric antigen receptor (CAR)-T cell therapy, which is partially caused by reduced accumulation of therapeutic T cells in tumor site. .. Unlike hematological malignancies, solid tumors have proved to be less susceptible to chimeric antigen receptor (CAR)-T cell therapy, which is partially caused by reduced accumulation of therapeutic T cells in tumor site.

    Gradient Centrifugation:

    Article Title: IL-27 attenuates airway inflammation in a mouse asthma model via the STAT1 and GADD45γ/p38 MAPK pathways
    Article Snippet: .. CD4+ T cell culture and IL-4 protein measurement For the human CD4+ T cells, peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Hypaque (Histopaque-1077; Sigma-Aldrich, St Louis, MO, USA) gradient centrifugation from the 15 mL of peripheral blood collected from each healthy volunteer. .. The CD4+ T cells were isolated from the PBMCs by means of magnetic bead separation (magnetic cell sorting) with a human CD4+ T Cell Isolation Kit (Miltenyi Biotec).

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    Millipore ceramide kinase antibody
    Metabolism of labeled C1P in C2C12 cells. C2C12 myoblasts, approximately 40% confluent, were serum-starved for 24 h and then exposed to 15 μM C1P (100000 dpm/well) for the indicated times. Cell lipids were extracted and analyzed by TLC as described in the Methods section. Results are expressed as the percentage of the radioactivity present in the indicated lipids, <t>ceramide</t> (Cer), fatty acid (FA), C1P and sphingomyelin (SM), compared to that in total lipids and are the mean ± SEM of six independent experiments performed in triplicate.
    Ceramide Kinase Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ceramide kinase antibody/product/Millipore
    Average 96 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ceramide kinase antibody - by Bioz Stars, 2020-09
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    93
    Millipore human pbmcs
    Imatinib inhibits TNF-α production in human myeloid cells. Human <t>PBMCs,</t> monocytes, or macrophages were exposed to either saline or imatinib (1 h, 1 μM) followed by 3-h stimulation with 100 ng/ml <t>LPS.</t> ( A ) LPS-induced TNF-α levels in supernatants of all three cell types as determined by ELISA ( n = 5; * , P ≤ 0.01). ( B and C ) Imatinib dose-dependently inhibits LPS-induced TNF-α protein ( B ) ( n = 5; * , P ≤ 0.01) and TNF-α mRNA expression ( C ) ( n = 3; * , P ≤ 0.01) in human PBMCs.
    Human Pbmcs, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pbmcs/product/Millipore
    Average 93 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    human pbmcs - by Bioz Stars, 2020-09
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    99
    Millipore peripheral blood mononuclear cells
    Generation, isolation, and characterization of epidermal growth factor receptor (EGFR)‐specific chimeric antigen receptor (CAR)‐engineered natural killer (NK) <t>cells</t> (EGFR‐CAR NK cells). (A) Flow cytometric analysis of phenotypic and subset composition of <t>peripheral</t> <t>blood</t> <t>mononuclear</t> cells (PBMCs) labeled with anti‐CD3‐PE‐Cy7, anti‐CD56‐PE. (B‐C) Flow cytometric analysis of phenotypic and subset composition of NK cells labeled with anti‐CD3‐PE‐Cy7, anti‐CD56‐PE, and anti‐CD69‐APC‐Cy7. (D) The percentage of CD3‐/CD56 + cells in day 0 PBMCs and day14 PBMC culture. (E) Real‐time PCR and (F) Western blotting analyses of the expression of exogenous CD3ζ in the non‐transduced NK cells, Con‐CAR NK cells, EGFR‐CAR‐1 NK cells, and EGFR‐CAR‐2 NK cells. β‐actin was used as an endogenous control. (G) The transduced NK cells stained with IgG‐FITC and EGFR‐FITC antibodies were detected by flow cytometry
    Peripheral Blood Mononuclear Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peripheral blood mononuclear cells/product/Millipore
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    peripheral blood mononuclear cells - by Bioz Stars, 2020-09
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    Image Search Results


    Metabolism of labeled C1P in C2C12 cells. C2C12 myoblasts, approximately 40% confluent, were serum-starved for 24 h and then exposed to 15 μM C1P (100000 dpm/well) for the indicated times. Cell lipids were extracted and analyzed by TLC as described in the Methods section. Results are expressed as the percentage of the radioactivity present in the indicated lipids, ceramide (Cer), fatty acid (FA), C1P and sphingomyelin (SM), compared to that in total lipids and are the mean ± SEM of six independent experiments performed in triplicate.

    Journal: Biochimie

    Article Title: Ceramide 1-phosphate stimulates proliferation of C2C12 myoblasts

    doi: 10.1016/j.biochi.2011.09.009

    Figure Lengend Snippet: Metabolism of labeled C1P in C2C12 cells. C2C12 myoblasts, approximately 40% confluent, were serum-starved for 24 h and then exposed to 15 μM C1P (100000 dpm/well) for the indicated times. Cell lipids were extracted and analyzed by TLC as described in the Methods section. Results are expressed as the percentage of the radioactivity present in the indicated lipids, ceramide (Cer), fatty acid (FA), C1P and sphingomyelin (SM), compared to that in total lipids and are the mean ± SEM of six independent experiments performed in triplicate.

    Article Snippet: Propidium iodide and the ceramide kinase antibody were from Calbiochem (San Diego, CA, USA).

    Techniques: Labeling, Thin Layer Chromatography, Radioactivity

    The effect of TGF-β on ceramide-induced death of Hs578T breast cancer cells. Graphs in ( A , C and E ) represent per cent dead cells while ( B ) and ( D ) represents per cent of cell in pre-G1. Where ( A ) and ( B ) Hs578T cells were pre-incubated with IGFBP-3 (100 ng ml −1 ) for 24 h followed by a co-incubation of IGFBP-3 and C2 (where C2 > CT; P

    Journal: British Journal of Cancer

    Article Title: Differential interactions between IGFBP-3 and transforming growth factor-beta (TGF-?) in normal vs cancerous breast epithelial cells

    doi: 10.1038/sj.bjc.6600355

    Figure Lengend Snippet: The effect of TGF-β on ceramide-induced death of Hs578T breast cancer cells. Graphs in ( A , C and E ) represent per cent dead cells while ( B ) and ( D ) represents per cent of cell in pre-G1. Where ( A ) and ( B ) Hs578T cells were pre-incubated with IGFBP-3 (100 ng ml −1 ) for 24 h followed by a co-incubation of IGFBP-3 and C2 (where C2 > CT; P

    Article Snippet: The ceramide analogue, C2, was purchased from Calbiochem (Nottingham, UK).

    Techniques: Incubation

    The effects of IGFBP-3 and TGF-β on ceramide-induced death of the MCF-10A cells. Graphs ( A + B ) represent the per cent of dead cells. MCF-10A cells were ( A ) pre-incubated with IGFBP-3 (100 ng ml −1 ) for 24 h followed by a co-incubation with an apoptotic dose of C2 for a further 24 h (where ***C2 > CT; P

    Journal: British Journal of Cancer

    Article Title: Differential interactions between IGFBP-3 and transforming growth factor-beta (TGF-?) in normal vs cancerous breast epithelial cells

    doi: 10.1038/sj.bjc.6600355

    Figure Lengend Snippet: The effects of IGFBP-3 and TGF-β on ceramide-induced death of the MCF-10A cells. Graphs ( A + B ) represent the per cent of dead cells. MCF-10A cells were ( A ) pre-incubated with IGFBP-3 (100 ng ml −1 ) for 24 h followed by a co-incubation with an apoptotic dose of C2 for a further 24 h (where ***C2 > CT; P

    Article Snippet: The ceramide analogue, C2, was purchased from Calbiochem (Nottingham, UK).

    Techniques: Incubation

    Imatinib inhibits TNF-α production in human myeloid cells. Human PBMCs, monocytes, or macrophages were exposed to either saline or imatinib (1 h, 1 μM) followed by 3-h stimulation with 100 ng/ml LPS. ( A ) LPS-induced TNF-α levels in supernatants of all three cell types as determined by ELISA ( n = 5; * , P ≤ 0.01). ( B and C ) Imatinib dose-dependently inhibits LPS-induced TNF-α protein ( B ) ( n = 5; * , P ≤ 0.01) and TNF-α mRNA expression ( C ) ( n = 3; * , P ≤ 0.01) in human PBMCs.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: The kinase inhibitor imatinib mesylate inhibits TNF-? production in vitro and prevents TNF-dependent acute hepatic inflammation

    doi: 10.1073/pnas.0501758102

    Figure Lengend Snippet: Imatinib inhibits TNF-α production in human myeloid cells. Human PBMCs, monocytes, or macrophages were exposed to either saline or imatinib (1 h, 1 μM) followed by 3-h stimulation with 100 ng/ml LPS. ( A ) LPS-induced TNF-α levels in supernatants of all three cell types as determined by ELISA ( n = 5; * , P ≤ 0.01). ( B and C ) Imatinib dose-dependently inhibits LPS-induced TNF-α protein ( B ) ( n = 5; * , P ≤ 0.01) and TNF-α mRNA expression ( C ) ( n = 3; * , P ≤ 0.01) in human PBMCs.

    Article Snippet: Human PBMCs or CD14-selected monocytes were stimulated with LPS at a concentration of 100 ng/ml for 12 h in the presence of either solvent or the indicated kinase inhibitor ( , SB203580, PD98059, and JNK-Inhibitor II, all used in a final concentration of 35 μM and purchased from Calbiochem).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing

    Generation, isolation, and characterization of epidermal growth factor receptor (EGFR)‐specific chimeric antigen receptor (CAR)‐engineered natural killer (NK) cells (EGFR‐CAR NK cells). (A) Flow cytometric analysis of phenotypic and subset composition of peripheral blood mononuclear cells (PBMCs) labeled with anti‐CD3‐PE‐Cy7, anti‐CD56‐PE. (B‐C) Flow cytometric analysis of phenotypic and subset composition of NK cells labeled with anti‐CD3‐PE‐Cy7, anti‐CD56‐PE, and anti‐CD69‐APC‐Cy7. (D) The percentage of CD3‐/CD56 + cells in day 0 PBMCs and day14 PBMC culture. (E) Real‐time PCR and (F) Western blotting analyses of the expression of exogenous CD3ζ in the non‐transduced NK cells, Con‐CAR NK cells, EGFR‐CAR‐1 NK cells, and EGFR‐CAR‐2 NK cells. β‐actin was used as an endogenous control. (G) The transduced NK cells stained with IgG‐FITC and EGFR‐FITC antibodies were detected by flow cytometry

    Journal: Cell Proliferation

    Article Title: Targeting epidermal growth factor‐overexpressing triple‐negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor, et al. Targeting epidermal growth factor‐overexpressing triple‐negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor

    doi: 10.1111/cpr.12858

    Figure Lengend Snippet: Generation, isolation, and characterization of epidermal growth factor receptor (EGFR)‐specific chimeric antigen receptor (CAR)‐engineered natural killer (NK) cells (EGFR‐CAR NK cells). (A) Flow cytometric analysis of phenotypic and subset composition of peripheral blood mononuclear cells (PBMCs) labeled with anti‐CD3‐PE‐Cy7, anti‐CD56‐PE. (B‐C) Flow cytometric analysis of phenotypic and subset composition of NK cells labeled with anti‐CD3‐PE‐Cy7, anti‐CD56‐PE, and anti‐CD69‐APC‐Cy7. (D) The percentage of CD3‐/CD56 + cells in day 0 PBMCs and day14 PBMC culture. (E) Real‐time PCR and (F) Western blotting analyses of the expression of exogenous CD3ζ in the non‐transduced NK cells, Con‐CAR NK cells, EGFR‐CAR‐1 NK cells, and EGFR‐CAR‐2 NK cells. β‐actin was used as an endogenous control. (G) The transduced NK cells stained with IgG‐FITC and EGFR‐FITC antibodies were detected by flow cytometry

    Article Snippet: Peripheral blood mononuclear cells were isolated from the whole blood of healthy donors by Ficoll density gradient centrifugation.

    Techniques: Isolation, Labeling, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Staining, Flow Cytometry