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    ZenBio human peripheral blood mononuclear cells pbmcs
    Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by ZenBio, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human peripheral blood mononuclear cells pbmcs/product/ZenBio
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
    92/100 stars

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    Article Title: The human long noncoding RNA, lnc-IL7R, regulates inflammatory response
    Article Snippet: Human peripheral blood mononuclear cells (PBMCs) were from Zen-Bio.

    Cell Culture:

    Article Title: miR-27a regulates inflammatory response of macrophages by targeting IL-10
    Article Snippet: .. Human peripheral blood mononuclear cells (PBMCs) were purchased from ZenBio Inc. PBMCs were cultured in DMEM media containing 10% FBS and 50 ng/ml human M-CSF (R & D Systems) for 5 days. ..

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    ZenBio bp lps treatment fresh healthy human pbmcs
    Cytokine secretion by human <t>PBMCs</t> in response to treatment with B . pseudomallei <t>LPS.</t> Levels of cytokines secreted by human PBMCs were measured at 2 and 24 h post LPS treatment for Donor 1 and 24 h post treatment from Donor 2. The cytokines analyzed are indicated above each plot with the sample origins indicated on the X-axis. Data for each sample set are divided by a dashed line. Mock treated negative control replicates are indicated by black circles, data obtained for Type A (1026b) LPS treatment are indicated by blue squares, and data obtained for Type B (576a) LPS treatment are indicated by green triangles. Salmon-colored triangles indicate data from positive control S . minnesota S-LPS treated samples. A), GM-CSF; B), IFN-γ; C), IL-1β; D), IL-2; E), IL-4; F), IL-5; G), IL-6; H), IL-8; I), IL-10; J), TNF-α. Scales are either linear or log depending on the cytokine for clarity. The means of all replicates and standard error of the means are indicated by error bars and lines. Differences between 576a compared to 1026b and Salmonella compared to 1026b treated were significant as determined by multiple one-way ANOVAs as shown above the datasets. ns = not significant, * = p
    Bp Lps Treatment Fresh Healthy Human Pbmcs, supplied by ZenBio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bp lps treatment fresh healthy human pbmcs/product/ZenBio
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bp lps treatment fresh healthy human pbmcs - by Bioz Stars, 2020-09
    91/100 stars
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    91
    ZenBio human pbmc gene expression analysis
    Cytokine secretion by human <t>PBMCs</t> in response to treatment with B . pseudomallei <t>LPS.</t> Levels of cytokines secreted by human PBMCs were measured at 2 and 24 h post LPS treatment for Donor 1 and 24 h post treatment from Donor 2. The cytokines analyzed are indicated above each plot with the sample origins indicated on the X-axis. Data for each sample set are divided by a dashed line. Mock treated negative control replicates are indicated by black circles, data obtained for Type A (1026b) LPS treatment are indicated by blue squares, and data obtained for Type B (576a) LPS treatment are indicated by green triangles. Salmon-colored triangles indicate data from positive control S . minnesota S-LPS treated samples. A), GM-CSF; B), IFN-γ; C), IL-1β; D), IL-2; E), IL-4; F), IL-5; G), IL-6; H), IL-8; I), IL-10; J), TNF-α. Scales are either linear or log depending on the cytokine for clarity. The means of all replicates and standard error of the means are indicated by error bars and lines. Differences between 576a compared to 1026b and Salmonella compared to 1026b treated were significant as determined by multiple one-way ANOVAs as shown above the datasets. ns = not significant, * = p
    Human Pbmc Gene Expression Analysis, supplied by ZenBio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pbmc gene expression analysis/product/ZenBio
    Average 91 stars, based on 1 article reviews
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    ZenBio pbmcs
    MicroRNA-183/96/182 is expressed in mouse Mϕ and <t>PMNs</t> ( A , B ) and in human <t>PBMCs</t> and PMNs ( C , D ). It is inactivated in the miR-183/96/182 ko mice ( A , B ). n = 3 for each group. **** P
    Pbmcs, supplied by ZenBio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs/product/ZenBio
    Average 92 stars, based on 1 article reviews
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    pbmcs - by Bioz Stars, 2020-09
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    ZenBio il 2 expanded human pbmcs
    The BCA2-mediated block on NF-κB delays proviral transcription and replication in CD4 + T cells. Jurkat CD4 + T cells (A) and ConA-activated and <t>IL-2-expanded</t> human <t>PBMCs</t> (B) were transduced with a vector encoding HA-BCA2 or treated with an empty vector (pQCXIP). Forty-eight hours later, the cells were infected with HIV-1 NL4.3. Proviral transcription was assessed at the selected time points by measuring nef RNA levels by RT-qPCR. (Right) The cell lysates derived from these experiments were analyzed by Western blotting for β-actin, BCA2, and p55/p24. (C) HIV-1 NL4.3 replication was also assessed in PBMCs transduced with an empty vector or HA-BCA2 by measuring the amounts of capsid p24 released to the culture supernatant at days 2, 4, 6, 8, and 10 postinfection. (D) The endogenous levels of BCA2 and β-actin were monitored over time in uninfected Jurkat CD4 + T cells and human PBMCs. The data correspond to the mean and standard deviation of three biological replicates, measured in technical replicates. Values that are significantly different are indicated by asterisks (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; n.s., not significant).
    Il 2 Expanded Human Pbmcs, supplied by ZenBio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1 article reviews
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    il 2 expanded human pbmcs - by Bioz Stars, 2020-09
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    Cytokine secretion by human PBMCs in response to treatment with B . pseudomallei LPS. Levels of cytokines secreted by human PBMCs were measured at 2 and 24 h post LPS treatment for Donor 1 and 24 h post treatment from Donor 2. The cytokines analyzed are indicated above each plot with the sample origins indicated on the X-axis. Data for each sample set are divided by a dashed line. Mock treated negative control replicates are indicated by black circles, data obtained for Type A (1026b) LPS treatment are indicated by blue squares, and data obtained for Type B (576a) LPS treatment are indicated by green triangles. Salmon-colored triangles indicate data from positive control S . minnesota S-LPS treated samples. A), GM-CSF; B), IFN-γ; C), IL-1β; D), IL-2; E), IL-4; F), IL-5; G), IL-6; H), IL-8; I), IL-10; J), TNF-α. Scales are either linear or log depending on the cytokine for clarity. The means of all replicates and standard error of the means are indicated by error bars and lines. Differences between 576a compared to 1026b and Salmonella compared to 1026b treated were significant as determined by multiple one-way ANOVAs as shown above the datasets. ns = not significant, * = p

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling

    doi: 10.1371/journal.pntd.0005571

    Figure Lengend Snippet: Cytokine secretion by human PBMCs in response to treatment with B . pseudomallei LPS. Levels of cytokines secreted by human PBMCs were measured at 2 and 24 h post LPS treatment for Donor 1 and 24 h post treatment from Donor 2. The cytokines analyzed are indicated above each plot with the sample origins indicated on the X-axis. Data for each sample set are divided by a dashed line. Mock treated negative control replicates are indicated by black circles, data obtained for Type A (1026b) LPS treatment are indicated by blue squares, and data obtained for Type B (576a) LPS treatment are indicated by green triangles. Salmon-colored triangles indicate data from positive control S . minnesota S-LPS treated samples. A), GM-CSF; B), IFN-γ; C), IL-1β; D), IL-2; E), IL-4; F), IL-5; G), IL-6; H), IL-8; I), IL-10; J), TNF-α. Scales are either linear or log depending on the cytokine for clarity. The means of all replicates and standard error of the means are indicated by error bars and lines. Differences between 576a compared to 1026b and Salmonella compared to 1026b treated were significant as determined by multiple one-way ANOVAs as shown above the datasets. ns = not significant, * = p

    Article Snippet: Human PBMC gene expression analysis in response to Bp LPS treatment Fresh healthy human PBMCs were purchased from ZenBio (North Carolina, USA).

    Techniques: Negative Control, Positive Control

    Dissimilar induction of innate and adaptive immunity by Type A and B LPS in human PBMCs. RT 2 qPCR arrays were used to look at differences between Type A and B LPS in their ability to induce innate and adaptive immunity in healthy human derived PBMCs. Heat maps of expression levels of innate and adaptive immune targets from human PBMCs of two donors after 24 h treatment in mock treated (-), Type A LPS (from 1026b) treated (A), and Type B LPS (from 576a) treated (B) PBMCs. Red is the highest fold change in expression and green is the least. Log 2 gene expression scatterplots are shown below the heat-maps.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling

    doi: 10.1371/journal.pntd.0005571

    Figure Lengend Snippet: Dissimilar induction of innate and adaptive immunity by Type A and B LPS in human PBMCs. RT 2 qPCR arrays were used to look at differences between Type A and B LPS in their ability to induce innate and adaptive immunity in healthy human derived PBMCs. Heat maps of expression levels of innate and adaptive immune targets from human PBMCs of two donors after 24 h treatment in mock treated (-), Type A LPS (from 1026b) treated (A), and Type B LPS (from 576a) treated (B) PBMCs. Red is the highest fold change in expression and green is the least. Log 2 gene expression scatterplots are shown below the heat-maps.

    Article Snippet: Human PBMC gene expression analysis in response to Bp LPS treatment Fresh healthy human PBMCs were purchased from ZenBio (North Carolina, USA).

    Techniques: Real-time Polymerase Chain Reaction, Derivative Assay, Expressing

    Cytokine secretion by human PBMCs in response to treatment with B . pseudomallei LPS. Levels of cytokines secreted by human PBMCs were measured at 2 and 24 h post LPS treatment for Donor 1 and 24 h post treatment from Donor 2. The cytokines analyzed are indicated above each plot with the sample origins indicated on the X-axis. Data for each sample set are divided by a dashed line. Mock treated negative control replicates are indicated by black circles, data obtained for Type A (1026b) LPS treatment are indicated by blue squares, and data obtained for Type B (576a) LPS treatment are indicated by green triangles. Salmon-colored triangles indicate data from positive control S . minnesota S-LPS treated samples. A), GM-CSF; B), IFN-γ; C), IL-1β; D), IL-2; E), IL-4; F), IL-5; G), IL-6; H), IL-8; I), IL-10; J), TNF-α. Scales are either linear or log depending on the cytokine for clarity. The means of all replicates and standard error of the means are indicated by error bars and lines. Differences between 576a compared to 1026b and Salmonella compared to 1026b treated were significant as determined by multiple one-way ANOVAs as shown above the datasets. ns = not significant, * = p

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling

    doi: 10.1371/journal.pntd.0005571

    Figure Lengend Snippet: Cytokine secretion by human PBMCs in response to treatment with B . pseudomallei LPS. Levels of cytokines secreted by human PBMCs were measured at 2 and 24 h post LPS treatment for Donor 1 and 24 h post treatment from Donor 2. The cytokines analyzed are indicated above each plot with the sample origins indicated on the X-axis. Data for each sample set are divided by a dashed line. Mock treated negative control replicates are indicated by black circles, data obtained for Type A (1026b) LPS treatment are indicated by blue squares, and data obtained for Type B (576a) LPS treatment are indicated by green triangles. Salmon-colored triangles indicate data from positive control S . minnesota S-LPS treated samples. A), GM-CSF; B), IFN-γ; C), IL-1β; D), IL-2; E), IL-4; F), IL-5; G), IL-6; H), IL-8; I), IL-10; J), TNF-α. Scales are either linear or log depending on the cytokine for clarity. The means of all replicates and standard error of the means are indicated by error bars and lines. Differences between 576a compared to 1026b and Salmonella compared to 1026b treated were significant as determined by multiple one-way ANOVAs as shown above the datasets. ns = not significant, * = p

    Article Snippet: Human PBMC gene expression analysis in response to Bp LPS treatment Fresh healthy human PBMCs were purchased from ZenBio (North Carolina, USA).

    Techniques: Negative Control, Positive Control

    Dissimilar induction of innate and adaptive immunity by Type A and B LPS in human PBMCs. RT 2 qPCR arrays were used to look at differences between Type A and B LPS in their ability to induce innate and adaptive immunity in healthy human derived PBMCs. Heat maps of expression levels of innate and adaptive immune targets from human PBMCs of two donors after 24 h treatment in mock treated (-), Type A LPS (from 1026b) treated (A), and Type B LPS (from 576a) treated (B) PBMCs. Red is the highest fold change in expression and green is the least. Log 2 gene expression scatterplots are shown below the heat-maps.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling

    doi: 10.1371/journal.pntd.0005571

    Figure Lengend Snippet: Dissimilar induction of innate and adaptive immunity by Type A and B LPS in human PBMCs. RT 2 qPCR arrays were used to look at differences between Type A and B LPS in their ability to induce innate and adaptive immunity in healthy human derived PBMCs. Heat maps of expression levels of innate and adaptive immune targets from human PBMCs of two donors after 24 h treatment in mock treated (-), Type A LPS (from 1026b) treated (A), and Type B LPS (from 576a) treated (B) PBMCs. Red is the highest fold change in expression and green is the least. Log 2 gene expression scatterplots are shown below the heat-maps.

    Article Snippet: Human PBMC gene expression analysis in response to Bp LPS treatment Fresh healthy human PBMCs were purchased from ZenBio (North Carolina, USA).

    Techniques: Real-time Polymerase Chain Reaction, Derivative Assay, Expressing

    MicroRNA-183/96/182 is expressed in mouse Mϕ and PMNs ( A , B ) and in human PBMCs and PMNs ( C , D ). It is inactivated in the miR-183/96/182 ko mice ( A , B ). n = 3 for each group. **** P

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Inactivation of the miR-183/96/182 Cluster Decreases the Severity of Pseudomonas aeruginosa-Induced Keratitis

    doi: 10.1167/iovs.16-19134

    Figure Lengend Snippet: MicroRNA-183/96/182 is expressed in mouse Mϕ and PMNs ( A , B ) and in human PBMCs and PMNs ( C , D ). It is inactivated in the miR-183/96/182 ko mice ( A , B ). n = 3 for each group. **** P

    Article Snippet: Human PMNs and PBMCs were purchased from Zen-Bio, Inc. (Research Triangle Park, NC, USA).

    Techniques: Mouse Assay

    The BCA2-mediated block on NF-κB delays proviral transcription and replication in CD4 + T cells. Jurkat CD4 + T cells (A) and ConA-activated and IL-2-expanded human PBMCs (B) were transduced with a vector encoding HA-BCA2 or treated with an empty vector (pQCXIP). Forty-eight hours later, the cells were infected with HIV-1 NL4.3. Proviral transcription was assessed at the selected time points by measuring nef RNA levels by RT-qPCR. (Right) The cell lysates derived from these experiments were analyzed by Western blotting for β-actin, BCA2, and p55/p24. (C) HIV-1 NL4.3 replication was also assessed in PBMCs transduced with an empty vector or HA-BCA2 by measuring the amounts of capsid p24 released to the culture supernatant at days 2, 4, 6, 8, and 10 postinfection. (D) The endogenous levels of BCA2 and β-actin were monitored over time in uninfected Jurkat CD4 + T cells and human PBMCs. The data correspond to the mean and standard deviation of three biological replicates, measured in technical replicates. Values that are significantly different are indicated by asterisks (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; n.s., not significant).

    Journal: Journal of Virology

    Article Title: BCA2/Rabring7 Interferes with HIV-1 Proviral Transcription by Enhancing the SUMOylation of IκBα

    doi: 10.1128/JVI.02098-16

    Figure Lengend Snippet: The BCA2-mediated block on NF-κB delays proviral transcription and replication in CD4 + T cells. Jurkat CD4 + T cells (A) and ConA-activated and IL-2-expanded human PBMCs (B) were transduced with a vector encoding HA-BCA2 or treated with an empty vector (pQCXIP). Forty-eight hours later, the cells were infected with HIV-1 NL4.3. Proviral transcription was assessed at the selected time points by measuring nef RNA levels by RT-qPCR. (Right) The cell lysates derived from these experiments were analyzed by Western blotting for β-actin, BCA2, and p55/p24. (C) HIV-1 NL4.3 replication was also assessed in PBMCs transduced with an empty vector or HA-BCA2 by measuring the amounts of capsid p24 released to the culture supernatant at days 2, 4, 6, 8, and 10 postinfection. (D) The endogenous levels of BCA2 and β-actin were monitored over time in uninfected Jurkat CD4 + T cells and human PBMCs. The data correspond to the mean and standard deviation of three biological replicates, measured in technical replicates. Values that are significantly different are indicated by asterisks (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; n.s., not significant).

    Article Snippet: Similar replication assays were performed in concanavalin A (ConA)-activated and IL-2-expanded human PBMCs (Zenbio Inc.) transduced with a retroviral empty vector (pQCXIP) or a retroviral vector encoding HA-BCA2.

    Techniques: Blocking Assay, Transduction, Plasmid Preparation, Infection, Quantitative RT-PCR, Derivative Assay, Western Blot, Standard Deviation