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Astarte Biologics human peripheral blood mononuclear cells pbmcs
Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human peripheral blood mononuclear cells pbmcs/product/Astarte Biologics
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
human peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
92/100 stars

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Article Title: EBV Positive Diffuse Large B Cell Lymphoma and Chronic Lymphocytic Leukemia Patients Exhibit Increased Anti-dUTPase Antibodies
Article Snippet: Human peripheral blood mononuclear cells (PBMCs) from healthy subjects were obtained from Astarte Biologics (Bothell, WA, USA).

Article Title: Human herpesviruses-encoded dUTPases: a family of proteins that modulate dendritic cell function and innate immunity
Article Snippet: Human peripheral blood mononuclear cells (PBMCs) from healthy subjects were obtained from Astarte Biologics (Cat# 1001 Lot # 1704OC12).

Article Title: Epstein-Barr Virus Encoded dUTPase Containing Exosomes Modulate Innate and Adaptive Immune Responses in Human Dendritic Cells and Peripheral Blood Mononuclear Cells
Article Snippet: Human peripheral blood mononuclear cells (PBMCs) from healthy subjects were obtained from Astarte Biologics (Cat# 1001 Lot # 1704OC12).

Article Title: Novel Adenovirus Vectors 'Capsid-Displaying' a Human Complement Inhibitor
Article Snippet: Adult normal human serum (NHS; pooled from 30 individuals) was purchased from Complement Technology (Tyler, Tex., USA); rhesus monkey serum was purchased from Innovative Research (Novi, Mich., USA), and human peripheral blood mononuclear cells (PBMCs) were obtained from Astarte Biologics (Redmond, Wash., USA).

Article Title: Peregrine
Article Snippet: Bacteria were revived with the addition of 300 μl of LB broth (Difco) followed by plating on LB agar (Difco) and incubation at 37°C overnight.

In Vitro:

Article Title: Gadolinium Contrast Agent-Induced CD163+ Ferroportin+ Osteogenic Cells in Nephrogenic Systemic Fibrosis
Article Snippet: .. For in vitro experiments, human peripheral blood mononuclear cells (PBMCs) were obtained from Astarte Biologics (Redmond, WA). ..

Cell Culture:

Article Title: Huachansu mediates cell death in non-Hodgkin’s lymphoma by induction of caspase-3 and inhibition of MAP kinase
Article Snippet: .. Human peripheral blood mononuclear cells (PBMCs) were purchased from Astarte Biologics (Redmond, WA, USA) and cultured in RPMI-1640 medium. ..

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  • 90
    Astarte Biologics hiv infection human pbmcs
    IFN-α is induced after <t>HIV-1</t> infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in <t>PBMCs</t> isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.
    Hiv Infection Human Pbmcs, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv infection human pbmcs/product/Astarte Biologics
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiv infection human pbmcs - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    88
    Astarte Biologics autologous apcs
    PV-267 specifically inhibits DR2- but not DR1-, DR4-, or DR9-mediated T cell responses. T cell enriched human <t>PBMCs</t> were co-cultured with PV-267 pre-treated autologous <t>APCs</t> and the indicated Ag and tested by human IFN-γ ELISPOT assay as described
    Autologous Apcs, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/autologous apcs/product/Astarte Biologics
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    autologous apcs - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    90
    Astarte Biologics human pbmc reconstitution
    IFN-α is induced after <t>HIV-1</t> infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in <t>PBMCs</t> isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.
    Human Pbmc Reconstitution, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pbmc reconstitution/product/Astarte Biologics
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human pbmc reconstitution - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    92
    Astarte Biologics human peripheral blood mononuclear cells pbmcs
    IFN-α is induced after <t>HIV-1</t> infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in <t>PBMCs</t> isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.
    Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human peripheral blood mononuclear cells pbmcs/product/Astarte Biologics
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    human peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    IFN-α is induced after HIV-1 infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in PBMCs isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.

    Journal: Oncotarget

    Article Title: Gene therapy with plasmids encoding IFN-β or IFN-α14 confers long-term resistance to HIV-1 in humanized mice

    doi: 10.18632/oncotarget.12512

    Figure Lengend Snippet: IFN-α is induced after HIV-1 infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in PBMCs isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.

    Article Snippet: Human PBMC reconstitution and HIV infection Human PBMCs were obtained from Astarte Biologics (Bothell, WA), and these cells (1×107 ) were injected through the tail vein into 6-8-week-old NSG mice.

    Techniques: Infection, Mouse Assay, Expressing, Enzyme-linked Immunosorbent Assay, Negative Control, Plasmid Preparation, Injection, Positive Control, Isolation, Quantitative RT-PCR, MANN-WHITNEY

    Injection of plasmids expressing subtypes of IFN-I results in prolonged upregulation of ISGs A. Hu-PBL mice were hydrodynamically injected with plasmids encoding IFN-α2, −α6, −α8, −α14 (left panel), or -IFN-beta (right panel) subtypes, and their sera were tested for IFN-I by ELISA at the indicated times after injection. n = 5 mice/group. B. PBMCs obtained on the indicated days after plasmid injection were tested for upregulation of the indicated ISGs over time by qRT-PCR. C. PBMCs obtained on days 2 and 40 after plasmid injection were tested for expression of indicated HIV-1 restriction factors by qPCR. Kruskal Wallis test followed by Dunn's multiple comparison analysis was used for statistical evaluation. * p

    Journal: Oncotarget

    Article Title: Gene therapy with plasmids encoding IFN-β or IFN-α14 confers long-term resistance to HIV-1 in humanized mice

    doi: 10.18632/oncotarget.12512

    Figure Lengend Snippet: Injection of plasmids expressing subtypes of IFN-I results in prolonged upregulation of ISGs A. Hu-PBL mice were hydrodynamically injected with plasmids encoding IFN-α2, −α6, −α8, −α14 (left panel), or -IFN-beta (right panel) subtypes, and their sera were tested for IFN-I by ELISA at the indicated times after injection. n = 5 mice/group. B. PBMCs obtained on the indicated days after plasmid injection were tested for upregulation of the indicated ISGs over time by qRT-PCR. C. PBMCs obtained on days 2 and 40 after plasmid injection were tested for expression of indicated HIV-1 restriction factors by qPCR. Kruskal Wallis test followed by Dunn's multiple comparison analysis was used for statistical evaluation. * p

    Article Snippet: Human PBMC reconstitution and HIV infection Human PBMCs were obtained from Astarte Biologics (Bothell, WA), and these cells (1×107 ) were injected through the tail vein into 6-8-week-old NSG mice.

    Techniques: Injection, Expressing, Mouse Assay, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    PV-267 specifically inhibits DR2- but not DR1-, DR4-, or DR9-mediated T cell responses. T cell enriched human PBMCs were co-cultured with PV-267 pre-treated autologous APCs and the indicated Ag and tested by human IFN-γ ELISPOT assay as described

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis

    doi: 10.4049/jimmunol.1300407

    Figure Lengend Snippet: PV-267 specifically inhibits DR2- but not DR1-, DR4-, or DR9-mediated T cell responses. T cell enriched human PBMCs were co-cultured with PV-267 pre-treated autologous APCs and the indicated Ag and tested by human IFN-γ ELISPOT assay as described

    Article Snippet: The staining was quenched by adding 5 × volumes of ice-cold culture media to the cells followed by incubation on ice for 5 min. CFSE labeled BC3 T cells were centrifuged and washed two times with culture media, then cultured with autologous APCs (irradiated human PBMCs, Astarte Biologics), 10 μg/ml of MBP87-99 peptide or mitogens such as PHA, anti-human CD3 mAb (OKT3) for 5 days at 37°C.

    Techniques: Cell Culture, Enzyme-linked Immunospot

    IFN-α is induced after HIV-1 infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in PBMCs isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.

    Journal: Oncotarget

    Article Title: Gene therapy with plasmids encoding IFN-β or IFN-α14 confers long-term resistance to HIV-1 in humanized mice

    doi: 10.18632/oncotarget.12512

    Figure Lengend Snippet: IFN-α is induced after HIV-1 infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in PBMCs isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.

    Article Snippet: Human PBMC reconstitution and HIV infection Human PBMCs were obtained from Astarte Biologics (Bothell, WA), and these cells (1×107 ) were injected through the tail vein into 6-8-week-old NSG mice.

    Techniques: Infection, Mouse Assay, Expressing, Enzyme-linked Immunosorbent Assay, Negative Control, Plasmid Preparation, Injection, Positive Control, Isolation, Quantitative RT-PCR, MANN-WHITNEY

    Injection of plasmids expressing subtypes of IFN-I results in prolonged upregulation of ISGs A. Hu-PBL mice were hydrodynamically injected with plasmids encoding IFN-α2, −α6, −α8, −α14 (left panel), or -IFN-beta (right panel) subtypes, and their sera were tested for IFN-I by ELISA at the indicated times after injection. n = 5 mice/group. B. PBMCs obtained on the indicated days after plasmid injection were tested for upregulation of the indicated ISGs over time by qRT-PCR. C. PBMCs obtained on days 2 and 40 after plasmid injection were tested for expression of indicated HIV-1 restriction factors by qPCR. Kruskal Wallis test followed by Dunn's multiple comparison analysis was used for statistical evaluation. * p

    Journal: Oncotarget

    Article Title: Gene therapy with plasmids encoding IFN-β or IFN-α14 confers long-term resistance to HIV-1 in humanized mice

    doi: 10.18632/oncotarget.12512

    Figure Lengend Snippet: Injection of plasmids expressing subtypes of IFN-I results in prolonged upregulation of ISGs A. Hu-PBL mice were hydrodynamically injected with plasmids encoding IFN-α2, −α6, −α8, −α14 (left panel), or -IFN-beta (right panel) subtypes, and their sera were tested for IFN-I by ELISA at the indicated times after injection. n = 5 mice/group. B. PBMCs obtained on the indicated days after plasmid injection were tested for upregulation of the indicated ISGs over time by qRT-PCR. C. PBMCs obtained on days 2 and 40 after plasmid injection were tested for expression of indicated HIV-1 restriction factors by qPCR. Kruskal Wallis test followed by Dunn's multiple comparison analysis was used for statistical evaluation. * p

    Article Snippet: Human PBMC reconstitution and HIV infection Human PBMCs were obtained from Astarte Biologics (Bothell, WA), and these cells (1×107 ) were injected through the tail vein into 6-8-week-old NSG mice.

    Techniques: Injection, Expressing, Mouse Assay, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction