human peripheral blood mononuclear cells pbmc  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Millipore human peripheral blood mononuclear cells pbmc
    Human CD4 + T cell immunogenicity of highly conserved class II immunogenic consensus sequences. In panel A, <t>PBMC</t> from 4 volunteers were stimulated in overnight IFN-γ ELISPOT assays with either DMSO alone (control) or FluICS peptides. In panel B, PBMC from 5 volunteers were expanded in vitro with DMSO (control), FluICS peptides, or live IAV for 1 week. Then CD4 + T cells were isolated and stimulated in IFN-γ ELISPOT assays with APC pulsed with DMSO, FluICS peptides, or live IAV (H1N1 and H3N2). These data demonstrate human immunogenicity of highly conserved influenza <t>ICS</t> and their relevance with infection with diverse IAV strains. Data depicted as means ± standard errors. # P
    Human Peripheral Blood Mononuclear Cells Pbmc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human peripheral blood mononuclear cells pbmc/product/Millipore
    Average 99 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    human peripheral blood mononuclear cells pbmc - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Highly Conserved Influenza T cell epitopes Induce Broadly Protective Immunity"

    Article Title: Highly Conserved Influenza T cell epitopes Induce Broadly Protective Immunity

    Journal: Vaccine

    doi: 10.1016/j.vaccine.2019.07.033

    Human CD4 + T cell immunogenicity of highly conserved class II immunogenic consensus sequences. In panel A, PBMC from 4 volunteers were stimulated in overnight IFN-γ ELISPOT assays with either DMSO alone (control) or FluICS peptides. In panel B, PBMC from 5 volunteers were expanded in vitro with DMSO (control), FluICS peptides, or live IAV for 1 week. Then CD4 + T cells were isolated and stimulated in IFN-γ ELISPOT assays with APC pulsed with DMSO, FluICS peptides, or live IAV (H1N1 and H3N2). These data demonstrate human immunogenicity of highly conserved influenza ICS and their relevance with infection with diverse IAV strains. Data depicted as means ± standard errors. # P
    Figure Legend Snippet: Human CD4 + T cell immunogenicity of highly conserved class II immunogenic consensus sequences. In panel A, PBMC from 4 volunteers were stimulated in overnight IFN-γ ELISPOT assays with either DMSO alone (control) or FluICS peptides. In panel B, PBMC from 5 volunteers were expanded in vitro with DMSO (control), FluICS peptides, or live IAV for 1 week. Then CD4 + T cells were isolated and stimulated in IFN-γ ELISPOT assays with APC pulsed with DMSO, FluICS peptides, or live IAV (H1N1 and H3N2). These data demonstrate human immunogenicity of highly conserved influenza ICS and their relevance with infection with diverse IAV strains. Data depicted as means ± standard errors. # P

    Techniques Used: Enzyme-linked Immunospot, In Vitro, Isolation, Infection

    2) Product Images from "Enhancement of hybridoma formation, clonability and cell proliferation in a nanoparticle-doped aqueous environment"

    Article Title: Enhancement of hybridoma formation, clonability and cell proliferation in a nanoparticle-doped aqueous environment

    Journal: BMC Biotechnology

    doi: 10.1186/1472-6750-8-3

    Fusion efficiency enhancement . The fusions were performed according to a standard protocol, where the culture media and PEG were reconstituted from powder forms with either NPD or DI water. For each fusion, PBMC from a single batch were divided into two equal fractures and used to prepare two parallel experiments, in NPD or DI based reagents. The figure presents percent of hybridoma-positive wells in each fusion experiment. The percent was calculated as the number of hybridoma-positive wells from 96-well plates where the cells were seeded and grown after the fusion process. The difference between the NPD- and DI-fusion results was found to be statistically significant by Chi-square analysis (p
    Figure Legend Snippet: Fusion efficiency enhancement . The fusions were performed according to a standard protocol, where the culture media and PEG were reconstituted from powder forms with either NPD or DI water. For each fusion, PBMC from a single batch were divided into two equal fractures and used to prepare two parallel experiments, in NPD or DI based reagents. The figure presents percent of hybridoma-positive wells in each fusion experiment. The percent was calculated as the number of hybridoma-positive wells from 96-well plates where the cells were seeded and grown after the fusion process. The difference between the NPD- and DI-fusion results was found to be statistically significant by Chi-square analysis (p

    Techniques Used:

    3) Product Images from "Apolipoprotein E4 Suppresses Neuronal-Specific Gene Expression in Maturing Neuronal Progenitor Cells Exposed to HIV"

    Article Title: Apolipoprotein E4 Suppresses Neuronal-Specific Gene Expression in Maturing Neuronal Progenitor Cells Exposed to HIV

    Journal: Journal of Neuroimmune Pharmacology

    doi: 10.1007/s11481-017-9734-9

    Detection of ApoE isoforms in differentiating hNP1 cells. Differentiating hNP1 cells were exposed to medium alone (Unt), mock-infected PBMC supernatants (Mock), and HIV-infected PBMC supernatants (HIV), and to rApoE3 or rApoE4 at 3 μg/ml, as described in Materials and Methods. At day 16 of differentiation, and 24 h after replenishment of rApoE in the cultures, cells were lysed for quantitative immunoblotting to detect ApoE. Lower blot depicts representative endogenous ApoE detected in cell culture lysates with molecular weight of 34 kDa, and rApoE with molecular weight of 36 kDa. Upper blot corresponds to beta-actin used as an internal control. For comparison, recombinant ApoE3 and ApoE4 were loaded directly on the gel at a 3 ng per lane, as shown. Bar graphs present ApoE signal normalized to beta-actin; each bar represents the mean of two experiments
    Figure Legend Snippet: Detection of ApoE isoforms in differentiating hNP1 cells. Differentiating hNP1 cells were exposed to medium alone (Unt), mock-infected PBMC supernatants (Mock), and HIV-infected PBMC supernatants (HIV), and to rApoE3 or rApoE4 at 3 μg/ml, as described in Materials and Methods. At day 16 of differentiation, and 24 h after replenishment of rApoE in the cultures, cells were lysed for quantitative immunoblotting to detect ApoE. Lower blot depicts representative endogenous ApoE detected in cell culture lysates with molecular weight of 34 kDa, and rApoE with molecular weight of 36 kDa. Upper blot corresponds to beta-actin used as an internal control. For comparison, recombinant ApoE3 and ApoE4 were loaded directly on the gel at a 3 ng per lane, as shown. Bar graphs present ApoE signal normalized to beta-actin; each bar represents the mean of two experiments

    Techniques Used: Infection, Cell Culture, Molecular Weight, Recombinant

    Related Articles

    Cell Isolation:

    Article Title: Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
    Article Snippet: .. Mononuclear cell isolation from spinal cords Mononuclear cells were isolated from spinal cords after cardiac perfusion with PBS, as described previously (11). .. Intracellular cytokine stainingThe number of Th17 cells in vivo was determined as described (12).

    In Vitro:

    Article Title: Dermotropic Leishmania donovani in Sri Lanka: visceralizing potential in clinical and preclinical studies
    Article Snippet: .. In vitro infection of human macrophages with L. donovani promastigotes Human mononuclear cells were isolated from peripheral blood of normal healthy donors by density gradient sedimentation in Ficoll-Hypaque (Sigma Chemical Co. St. Louis, MO). ..

    Mutagenesis:

    Article Title: Structural basis for abrogated binding between staphylococcal enterotoxin A superantigen vaccine and MHC-II?
    Article Snippet: .. Human peripheral blood mononuclear cells were purified by Ficoll-hypaque (Sigma) buoyant density gradient centrifugation and cultured with wild-type or mutant SEA in RPMI-1640 with 5% fetal bovine serum. .. Following 72 h of culture, the mononuclear cells were pulse-labeled for 12 h with 1μCi [3H]-thymidine (Amersham) and harvested onto glass-fiber filters.

    Isolation:

    Article Title: Dermotropic Leishmania donovani in Sri Lanka: visceralizing potential in clinical and preclinical studies
    Article Snippet: .. In vitro infection of human macrophages with L. donovani promastigotes Human mononuclear cells were isolated from peripheral blood of normal healthy donors by density gradient sedimentation in Ficoll-Hypaque (Sigma Chemical Co. St. Louis, MO). ..

    Article Title: Human dental follicle mesenchymal stem cells alleviate T cell response in inflamed tissue of Crohn’s patients
    Article Snippet: .. Mononuclear cells isolation from inflamed and noninflamed tissueCell isolation was carried out following a previously described protocol ( ). ..

    Article Title: Small‐molecule Hedgehog inhibitor attenuates the leukemia‐initiation potential of acute myeloid leukemia cells
    Article Snippet: .. Colony formation analysis Human mononuclear cells isolated from AML patients were thawed, washed with ISCOV (Sigma) containing 2% FBS, and leukemic colony formation assays were carried out. .. Leukemia cells (2 × 106 to 1 × 107 ) were added at a dilution of 1:10 to the methylcellulose medium MethoCult H4534 (StemCell Technologies, Vancouver, Canada).

    Article Title: Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
    Article Snippet: .. Mononuclear cell isolation from spinal cords Mononuclear cells were isolated from spinal cords after cardiac perfusion with PBS, as described previously (11). .. Intracellular cytokine stainingThe number of Th17 cells in vivo was determined as described (12).

    Article Title: Anti-CD2 producing pig xenografts effect localized depletion of human T cells in a huSCID model.
    Article Snippet: .. We investigated whether graft produced anti-human CD2, mediated by adenovirus (Adv) transduction of pig neonatal islet cell clusters (pNICC), would protect xenografts in a humanized mouse model from immune attack and whether such immunosuppression would remain local. ..

    Article Title: MicroRNA expression profiling of Leishmania donovani-infected host cells uncovers the regulatory role of MIR30A-3p in host autophagy
    Article Snippet: .. Primary human peripheral blood mononuclear cells were isolated using a Ficoll Paque density gradient (Sigma-Aldrich, GE-17-5442-02) on whole blood collected from healthy donors. .. Human monocyte-derived macrophages (HsMDMs) were isolated as described previously.

    Infection:

    Article Title: Dermotropic Leishmania donovani in Sri Lanka: visceralizing potential in clinical and preclinical studies
    Article Snippet: .. In vitro infection of human macrophages with L. donovani promastigotes Human mononuclear cells were isolated from peripheral blood of normal healthy donors by density gradient sedimentation in Ficoll-Hypaque (Sigma Chemical Co. St. Louis, MO). ..

    Purification:

    Article Title: Structural basis for abrogated binding between staphylococcal enterotoxin A superantigen vaccine and MHC-II?
    Article Snippet: .. Human peripheral blood mononuclear cells were purified by Ficoll-hypaque (Sigma) buoyant density gradient centrifugation and cultured with wild-type or mutant SEA in RPMI-1640 with 5% fetal bovine serum. .. Following 72 h of culture, the mononuclear cells were pulse-labeled for 12 h with 1μCi [3H]-thymidine (Amersham) and harvested onto glass-fiber filters.

    Sedimentation:

    Article Title: Dermotropic Leishmania donovani in Sri Lanka: visceralizing potential in clinical and preclinical studies
    Article Snippet: .. In vitro infection of human macrophages with L. donovani promastigotes Human mononuclear cells were isolated from peripheral blood of normal healthy donors by density gradient sedimentation in Ficoll-Hypaque (Sigma Chemical Co. St. Louis, MO). ..

    Cell Culture:

    Article Title: Structural basis for abrogated binding between staphylococcal enterotoxin A superantigen vaccine and MHC-II?
    Article Snippet: .. Human peripheral blood mononuclear cells were purified by Ficoll-hypaque (Sigma) buoyant density gradient centrifugation and cultured with wild-type or mutant SEA in RPMI-1640 with 5% fetal bovine serum. .. Following 72 h of culture, the mononuclear cells were pulse-labeled for 12 h with 1μCi [3H]-thymidine (Amersham) and harvested onto glass-fiber filters.

    Gradient Centrifugation:

    Article Title: Structural basis for abrogated binding between staphylococcal enterotoxin A superantigen vaccine and MHC-II?
    Article Snippet: .. Human peripheral blood mononuclear cells were purified by Ficoll-hypaque (Sigma) buoyant density gradient centrifugation and cultured with wild-type or mutant SEA in RPMI-1640 with 5% fetal bovine serum. .. Following 72 h of culture, the mononuclear cells were pulse-labeled for 12 h with 1μCi [3H]-thymidine (Amersham) and harvested onto glass-fiber filters.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Millipore ceramide kinase antibody
    Metabolism of labeled C1P in C2C12 cells. C2C12 myoblasts, approximately 40% confluent, were serum-starved for 24 h and then exposed to 15 μM C1P (100000 dpm/well) for the indicated times. Cell lipids were extracted and analyzed by TLC as described in the Methods section. Results are expressed as the percentage of the radioactivity present in the indicated lipids, <t>ceramide</t> (Cer), fatty acid (FA), C1P and sphingomyelin (SM), compared to that in total lipids and are the mean ± SEM of six independent experiments performed in triplicate.
    Ceramide Kinase Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ceramide kinase antibody/product/Millipore
    Average 96 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ceramide kinase antibody - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    93
    Millipore human pbmcs
    Imatinib inhibits TNF-α production in human myeloid cells. Human <t>PBMCs,</t> monocytes, or macrophages were exposed to either saline or imatinib (1 h, 1 μM) followed by 3-h stimulation with 100 ng/ml <t>LPS.</t> ( A ) LPS-induced TNF-α levels in supernatants of all three cell types as determined by ELISA ( n = 5; * , P ≤ 0.01). ( B and C ) Imatinib dose-dependently inhibits LPS-induced TNF-α protein ( B ) ( n = 5; * , P ≤ 0.01) and TNF-α mRNA expression ( C ) ( n = 3; * , P ≤ 0.01) in human PBMCs.
    Human Pbmcs, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pbmcs/product/Millipore
    Average 93 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    human pbmcs - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    99
    Millipore peripheral blood mononuclear cells
    Generation, isolation, and characterization of epidermal growth factor receptor (EGFR)‐specific chimeric antigen receptor (CAR)‐engineered natural killer (NK) <t>cells</t> (EGFR‐CAR NK cells). (A) Flow cytometric analysis of phenotypic and subset composition of <t>peripheral</t> <t>blood</t> <t>mononuclear</t> cells (PBMCs) labeled with anti‐CD3‐PE‐Cy7, anti‐CD56‐PE. (B‐C) Flow cytometric analysis of phenotypic and subset composition of NK cells labeled with anti‐CD3‐PE‐Cy7, anti‐CD56‐PE, and anti‐CD69‐APC‐Cy7. (D) The percentage of CD3‐/CD56 + cells in day 0 PBMCs and day14 PBMC culture. (E) Real‐time PCR and (F) Western blotting analyses of the expression of exogenous CD3ζ in the non‐transduced NK cells, Con‐CAR NK cells, EGFR‐CAR‐1 NK cells, and EGFR‐CAR‐2 NK cells. β‐actin was used as an endogenous control. (G) The transduced NK cells stained with IgG‐FITC and EGFR‐FITC antibodies were detected by flow cytometry
    Peripheral Blood Mononuclear Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peripheral blood mononuclear cells/product/Millipore
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    peripheral blood mononuclear cells - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Metabolism of labeled C1P in C2C12 cells. C2C12 myoblasts, approximately 40% confluent, were serum-starved for 24 h and then exposed to 15 μM C1P (100000 dpm/well) for the indicated times. Cell lipids were extracted and analyzed by TLC as described in the Methods section. Results are expressed as the percentage of the radioactivity present in the indicated lipids, ceramide (Cer), fatty acid (FA), C1P and sphingomyelin (SM), compared to that in total lipids and are the mean ± SEM of six independent experiments performed in triplicate.

    Journal: Biochimie

    Article Title: Ceramide 1-phosphate stimulates proliferation of C2C12 myoblasts

    doi: 10.1016/j.biochi.2011.09.009

    Figure Lengend Snippet: Metabolism of labeled C1P in C2C12 cells. C2C12 myoblasts, approximately 40% confluent, were serum-starved for 24 h and then exposed to 15 μM C1P (100000 dpm/well) for the indicated times. Cell lipids were extracted and analyzed by TLC as described in the Methods section. Results are expressed as the percentage of the radioactivity present in the indicated lipids, ceramide (Cer), fatty acid (FA), C1P and sphingomyelin (SM), compared to that in total lipids and are the mean ± SEM of six independent experiments performed in triplicate.

    Article Snippet: Propidium iodide and the ceramide kinase antibody were from Calbiochem (San Diego, CA, USA).

    Techniques: Labeling, Thin Layer Chromatography, Radioactivity

    The effect of TGF-β on ceramide-induced death of Hs578T breast cancer cells. Graphs in ( A , C and E ) represent per cent dead cells while ( B ) and ( D ) represents per cent of cell in pre-G1. Where ( A ) and ( B ) Hs578T cells were pre-incubated with IGFBP-3 (100 ng ml −1 ) for 24 h followed by a co-incubation of IGFBP-3 and C2 (where C2 > CT; P

    Journal: British Journal of Cancer

    Article Title: Differential interactions between IGFBP-3 and transforming growth factor-beta (TGF-?) in normal vs cancerous breast epithelial cells

    doi: 10.1038/sj.bjc.6600355

    Figure Lengend Snippet: The effect of TGF-β on ceramide-induced death of Hs578T breast cancer cells. Graphs in ( A , C and E ) represent per cent dead cells while ( B ) and ( D ) represents per cent of cell in pre-G1. Where ( A ) and ( B ) Hs578T cells were pre-incubated with IGFBP-3 (100 ng ml −1 ) for 24 h followed by a co-incubation of IGFBP-3 and C2 (where C2 > CT; P

    Article Snippet: The ceramide analogue, C2, was purchased from Calbiochem (Nottingham, UK).

    Techniques: Incubation

    The effects of IGFBP-3 and TGF-β on ceramide-induced death of the MCF-10A cells. Graphs ( A + B ) represent the per cent of dead cells. MCF-10A cells were ( A ) pre-incubated with IGFBP-3 (100 ng ml −1 ) for 24 h followed by a co-incubation with an apoptotic dose of C2 for a further 24 h (where ***C2 > CT; P

    Journal: British Journal of Cancer

    Article Title: Differential interactions between IGFBP-3 and transforming growth factor-beta (TGF-?) in normal vs cancerous breast epithelial cells

    doi: 10.1038/sj.bjc.6600355

    Figure Lengend Snippet: The effects of IGFBP-3 and TGF-β on ceramide-induced death of the MCF-10A cells. Graphs ( A + B ) represent the per cent of dead cells. MCF-10A cells were ( A ) pre-incubated with IGFBP-3 (100 ng ml −1 ) for 24 h followed by a co-incubation with an apoptotic dose of C2 for a further 24 h (where ***C2 > CT; P

    Article Snippet: The ceramide analogue, C2, was purchased from Calbiochem (Nottingham, UK).

    Techniques: Incubation

    Imatinib inhibits TNF-α production in human myeloid cells. Human PBMCs, monocytes, or macrophages were exposed to either saline or imatinib (1 h, 1 μM) followed by 3-h stimulation with 100 ng/ml LPS. ( A ) LPS-induced TNF-α levels in supernatants of all three cell types as determined by ELISA ( n = 5; * , P ≤ 0.01). ( B and C ) Imatinib dose-dependently inhibits LPS-induced TNF-α protein ( B ) ( n = 5; * , P ≤ 0.01) and TNF-α mRNA expression ( C ) ( n = 3; * , P ≤ 0.01) in human PBMCs.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: The kinase inhibitor imatinib mesylate inhibits TNF-? production in vitro and prevents TNF-dependent acute hepatic inflammation

    doi: 10.1073/pnas.0501758102

    Figure Lengend Snippet: Imatinib inhibits TNF-α production in human myeloid cells. Human PBMCs, monocytes, or macrophages were exposed to either saline or imatinib (1 h, 1 μM) followed by 3-h stimulation with 100 ng/ml LPS. ( A ) LPS-induced TNF-α levels in supernatants of all three cell types as determined by ELISA ( n = 5; * , P ≤ 0.01). ( B and C ) Imatinib dose-dependently inhibits LPS-induced TNF-α protein ( B ) ( n = 5; * , P ≤ 0.01) and TNF-α mRNA expression ( C ) ( n = 3; * , P ≤ 0.01) in human PBMCs.

    Article Snippet: Human PBMCs or CD14-selected monocytes were stimulated with LPS at a concentration of 100 ng/ml for 12 h in the presence of either solvent or the indicated kinase inhibitor ( , SB203580, PD98059, and JNK-Inhibitor II, all used in a final concentration of 35 μM and purchased from Calbiochem).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing

    Generation, isolation, and characterization of epidermal growth factor receptor (EGFR)‐specific chimeric antigen receptor (CAR)‐engineered natural killer (NK) cells (EGFR‐CAR NK cells). (A) Flow cytometric analysis of phenotypic and subset composition of peripheral blood mononuclear cells (PBMCs) labeled with anti‐CD3‐PE‐Cy7, anti‐CD56‐PE. (B‐C) Flow cytometric analysis of phenotypic and subset composition of NK cells labeled with anti‐CD3‐PE‐Cy7, anti‐CD56‐PE, and anti‐CD69‐APC‐Cy7. (D) The percentage of CD3‐/CD56 + cells in day 0 PBMCs and day14 PBMC culture. (E) Real‐time PCR and (F) Western blotting analyses of the expression of exogenous CD3ζ in the non‐transduced NK cells, Con‐CAR NK cells, EGFR‐CAR‐1 NK cells, and EGFR‐CAR‐2 NK cells. β‐actin was used as an endogenous control. (G) The transduced NK cells stained with IgG‐FITC and EGFR‐FITC antibodies were detected by flow cytometry

    Journal: Cell Proliferation

    Article Title: Targeting epidermal growth factor‐overexpressing triple‐negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor, et al. Targeting epidermal growth factor‐overexpressing triple‐negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor

    doi: 10.1111/cpr.12858

    Figure Lengend Snippet: Generation, isolation, and characterization of epidermal growth factor receptor (EGFR)‐specific chimeric antigen receptor (CAR)‐engineered natural killer (NK) cells (EGFR‐CAR NK cells). (A) Flow cytometric analysis of phenotypic and subset composition of peripheral blood mononuclear cells (PBMCs) labeled with anti‐CD3‐PE‐Cy7, anti‐CD56‐PE. (B‐C) Flow cytometric analysis of phenotypic and subset composition of NK cells labeled with anti‐CD3‐PE‐Cy7, anti‐CD56‐PE, and anti‐CD69‐APC‐Cy7. (D) The percentage of CD3‐/CD56 + cells in day 0 PBMCs and day14 PBMC culture. (E) Real‐time PCR and (F) Western blotting analyses of the expression of exogenous CD3ζ in the non‐transduced NK cells, Con‐CAR NK cells, EGFR‐CAR‐1 NK cells, and EGFR‐CAR‐2 NK cells. β‐actin was used as an endogenous control. (G) The transduced NK cells stained with IgG‐FITC and EGFR‐FITC antibodies were detected by flow cytometry

    Article Snippet: Peripheral blood mononuclear cells were isolated from the whole blood of healthy donors by Ficoll density gradient centrifugation.

    Techniques: Isolation, Labeling, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Staining, Flow Cytometry