human peripheral blood mononuclear cells pbmc  (AllCells LLC)

 
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    AllCells LLC human peripheral blood mononuclear cells pbmc
    Human Peripheral Blood Mononuclear Cells Pbmc, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human peripheral blood mononuclear cells pbmc/product/AllCells LLC
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human peripheral blood mononuclear cells pbmc - by Bioz Stars, 2020-09
    92/100 stars

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    Isolation:

    Article Title: An efficient Screening System in Yeast to Select a Hyperactive piggyBac Transposase for Mammalian Applications
    Article Snippet: .. The human peripheral blood mononuclear cells (PBMC) of healthy donors were purchased from AllCells (AllCells, Silicon Valley, CA, USA) under a protocol approved by the Ethics Committee of Chinese People’s Liberation Army General Hospital (S2019-063-01), and isolated from heparinized blood using Ficoll-Paque density gradient centrifugation. .. PBMCs were plated in serum free AIM-V media (Life Technologies, Grand Island, NY, USA) and allowed to adhere to 0.22 μm filter-capped culture flasks (Corning, Corning, NY, USA).

    Article Title: Antiviral Activity of Bictegravir (GS-9883), a Novel Potent HIV-1 Integrase Strand Transfer Inhibitor with an Improved Resistance Profile
    Article Snippet: .. Freshly isolated human peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers (supplied by AllCells, Inc., Alameda, CA). ..

    Article Title: Discovery and preclinical characterization of the antagonist anti-PD-L1 monoclonal antibody LY3300054
    Article Snippet: .. Mixed leukocyte reaction (MLR) CD14+ monocytes were isolated from frozen human peripheral blood mononuclear cells (PBMC) obtained from a healthy donor (AllCells, Alameda, CA) with Human Monocyte Isolation Kit II (Miltenyi, Auburn, CA). .. Immature dendritic cells (DCs) were generated by culturing these monocytes in complete RPMI-1640 medium containing 10% FBS in the presence of 1000 IU/ml hGM-CSF and 500 IU/ml hIL-4 for 4 days.

    Gradient Centrifugation:

    Article Title: An efficient Screening System in Yeast to Select a Hyperactive piggyBac Transposase for Mammalian Applications
    Article Snippet: .. The human peripheral blood mononuclear cells (PBMC) of healthy donors were purchased from AllCells (AllCells, Silicon Valley, CA, USA) under a protocol approved by the Ethics Committee of Chinese People’s Liberation Army General Hospital (S2019-063-01), and isolated from heparinized blood using Ficoll-Paque density gradient centrifugation. .. PBMCs were plated in serum free AIM-V media (Life Technologies, Grand Island, NY, USA) and allowed to adhere to 0.22 μm filter-capped culture flasks (Corning, Corning, NY, USA).

    other:

    Article Title: EGR2 is elevated and positively regulates inflammatory IFNγ production in lupus CD4+ T cells
    Article Snippet: Human peripheral blood mononuclear cells (PBMCs) The PBMCs of human patients of lupus (n = 4, all female) and healthy controls (n = 4, 2 male and 2 female) were purchased directly from AllCells LLC (Alameda, CA, USA).

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    AllCells LLC adcc assay human peripheral blood mononuclear cells pbmc
    <t>ADCC</t> of Y-443 and Fc-mutated antibodies against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with Calcein AM were incubated with different concentrations of Y-443 or the Fc mutants, followed by addition of <t>PBMC</t> effector cells at a ratio of 1:50. The cell mixture was incubated for 4 hours at 37°C, and Calcein AM intensity in cells was detected by Acumen eX3 (TTP labtech). The results are the mean ± S.D. of dead cell ratio.
    Adcc Assay Human Peripheral Blood Mononuclear Cells Pbmc, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adcc assay human peripheral blood mononuclear cells pbmc/product/AllCells LLC
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    adcc assay human peripheral blood mononuclear cells pbmc - by Bioz Stars, 2020-09
    85/100 stars
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    92
    AllCells LLC pbmcs
    HIV-1 infection in humanized mice passively infused with b12-IgA or b12-IgG isotype. (A) Neutralization activity of anti-HIV human mAb b12-IgA. Reporter cell line TZM-bl cells that express CD4, CXCR4, CCR5, and a Tat-responsive reporter gene for luciferase were infected with 200 TCID 50 of replication-defective pseudovirus containing Env (SF162.LS) in the presence of various concentrations of anti-HIV monoclonal antibodies. Neutralization activity was measured by the reduction in luciferase reporter gene expression after a single round of pseudovirus infection in TZM-bl cells in triplicate. b12-IgG1 and the recombinant b12-IgA2 were compared at indicated concentrations. (B) Antibody level in circulation after passive transfer. <t>NSG-hu</t> mice were injected intravenously with various concentrations of purified b12-IgA2 (pIgA: mIgA = 1:1, mass ratio) or b12-IgG1 and the blood was collected after 4 hours. The plasma antibody concentrations were measured using ELISA. (n = 4-6, mean ± SEM). (C-D) Concentrations of b12 antibodies in plasma (C) or genital secretions (D) at the time of challenge. NSG-hu mice were injected intravenously with either 200 μg of b12-IgA2 or 20 μg of b12-IgG1 per mouse. Blood and genital secretions were collected after 4 hours. (E-I) Peripheral CD4 + T cell loss after HIV-1 challenge in NSG-hu mice injected with different b12 antibody isotypes; (E) purified human IgG/κ control antibody (hIgG/κ); (F) b12-IgA2 includes both monomeric and polymeric IgA as described in (B), (b12IgA2 [M+P]); (G) b12-IgG1; (H) b12-IgA2 monomer only (b12IgA2 [M]). Mice were challenged intravaginally with HIV-1 JR-CSF . (I) Average percent CD4 + T cells in CD3 T cells in <t>PBMCs</t> at each time points (n = 5-9). Statistical analysis was performed using unpaired t test. (* P = .0313 and *** P
    Pbmcs, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs/product/AllCells LLC
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pbmcs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    86
    AllCells LLC hiv latency total peripheral blood mononuclear cells pbmcs
    RMD does not induce global activation of immune cell subsets. <t>PBMCs</t> isolated from four <t>HIV-infected</t> patients on suppressive cART were treated with a 4-hour pulse of RMD or continuously with vehicle control (DMSO), VOR, or PMA+ionomycin and stained for surface markers 48 hours after the treatment initiation. Fractions of CD69−, CD25−, and HLA-DR-positive cells in subsets of CD4+ T cells, CD8+ T cells, and CD19+ B cells were analyzed by flow cytometry as described in Materials and Methods . Each symbol represents one donor.
    Hiv Latency Total Peripheral Blood Mononuclear Cells Pbmcs, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv latency total peripheral blood mononuclear cells pbmcs/product/AllCells LLC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiv latency total peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
    86/100 stars
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    ADCC of Y-443 and Fc-mutated antibodies against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with Calcein AM were incubated with different concentrations of Y-443 or the Fc mutants, followed by addition of PBMC effector cells at a ratio of 1:50. The cell mixture was incubated for 4 hours at 37°C, and Calcein AM intensity in cells was detected by Acumen eX3 (TTP labtech). The results are the mean ± S.D. of dead cell ratio.

    Journal: PLoS ONE

    Article Title: Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey

    doi: 10.1371/journal.pone.0196422

    Figure Lengend Snippet: ADCC of Y-443 and Fc-mutated antibodies against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with Calcein AM were incubated with different concentrations of Y-443 or the Fc mutants, followed by addition of PBMC effector cells at a ratio of 1:50. The cell mixture was incubated for 4 hours at 37°C, and Calcein AM intensity in cells was detected by Acumen eX3 (TTP labtech). The results are the mean ± S.D. of dead cell ratio.

    Article Snippet: ADCC assay Human peripheral blood mononuclear cells (PBMC) purchased from AllCells, LLC and were cultured in RPMI1640 medium containing 10% fetal bovine serum, 0.1 nM human IL-2 (DIACLONE Research) and 55 μM 2-mercaptoethanol for 24 hours.

    Techniques: Multiple Displacement Amplification, Labeling, Incubation

    HIV-1 infection in humanized mice passively infused with b12-IgA or b12-IgG isotype. (A) Neutralization activity of anti-HIV human mAb b12-IgA. Reporter cell line TZM-bl cells that express CD4, CXCR4, CCR5, and a Tat-responsive reporter gene for luciferase were infected with 200 TCID 50 of replication-defective pseudovirus containing Env (SF162.LS) in the presence of various concentrations of anti-HIV monoclonal antibodies. Neutralization activity was measured by the reduction in luciferase reporter gene expression after a single round of pseudovirus infection in TZM-bl cells in triplicate. b12-IgG1 and the recombinant b12-IgA2 were compared at indicated concentrations. (B) Antibody level in circulation after passive transfer. NSG-hu mice were injected intravenously with various concentrations of purified b12-IgA2 (pIgA: mIgA = 1:1, mass ratio) or b12-IgG1 and the blood was collected after 4 hours. The plasma antibody concentrations were measured using ELISA. (n = 4-6, mean ± SEM). (C-D) Concentrations of b12 antibodies in plasma (C) or genital secretions (D) at the time of challenge. NSG-hu mice were injected intravenously with either 200 μg of b12-IgA2 or 20 μg of b12-IgG1 per mouse. Blood and genital secretions were collected after 4 hours. (E-I) Peripheral CD4 + T cell loss after HIV-1 challenge in NSG-hu mice injected with different b12 antibody isotypes; (E) purified human IgG/κ control antibody (hIgG/κ); (F) b12-IgA2 includes both monomeric and polymeric IgA as described in (B), (b12IgA2 [M+P]); (G) b12-IgG1; (H) b12-IgA2 monomer only (b12IgA2 [M]). Mice were challenged intravaginally with HIV-1 JR-CSF . (I) Average percent CD4 + T cells in CD3 T cells in PBMCs at each time points (n = 5-9). Statistical analysis was performed using unpaired t test. (* P = .0313 and *** P

    Journal: Blood

    Article Title: Inhibitory effect of HIV-specific neutralizing IgA on mucosal transmission of HIV in humanized mice

    doi: 10.1182/blood-2012-04-422303

    Figure Lengend Snippet: HIV-1 infection in humanized mice passively infused with b12-IgA or b12-IgG isotype. (A) Neutralization activity of anti-HIV human mAb b12-IgA. Reporter cell line TZM-bl cells that express CD4, CXCR4, CCR5, and a Tat-responsive reporter gene for luciferase were infected with 200 TCID 50 of replication-defective pseudovirus containing Env (SF162.LS) in the presence of various concentrations of anti-HIV monoclonal antibodies. Neutralization activity was measured by the reduction in luciferase reporter gene expression after a single round of pseudovirus infection in TZM-bl cells in triplicate. b12-IgG1 and the recombinant b12-IgA2 were compared at indicated concentrations. (B) Antibody level in circulation after passive transfer. NSG-hu mice were injected intravenously with various concentrations of purified b12-IgA2 (pIgA: mIgA = 1:1, mass ratio) or b12-IgG1 and the blood was collected after 4 hours. The plasma antibody concentrations were measured using ELISA. (n = 4-6, mean ± SEM). (C-D) Concentrations of b12 antibodies in plasma (C) or genital secretions (D) at the time of challenge. NSG-hu mice were injected intravenously with either 200 μg of b12-IgA2 or 20 μg of b12-IgG1 per mouse. Blood and genital secretions were collected after 4 hours. (E-I) Peripheral CD4 + T cell loss after HIV-1 challenge in NSG-hu mice injected with different b12 antibody isotypes; (E) purified human IgG/κ control antibody (hIgG/κ); (F) b12-IgA2 includes both monomeric and polymeric IgA as described in (B), (b12IgA2 [M+P]); (G) b12-IgG1; (H) b12-IgA2 monomer only (b12IgA2 [M]). Mice were challenged intravaginally with HIV-1 JR-CSF . (I) Average percent CD4 + T cells in CD3 T cells in PBMCs at each time points (n = 5-9). Statistical analysis was performed using unpaired t test. (* P = .0313 and *** P

    Article Snippet: For passive antibody transfer experiments, NOD.Cg- Prkd scid IL2rg tm1Wjl /SzJ (NOD/SCID IL2r γ−/− , NSG) mice were transplanted intraperitoneally with 3 × 106 human PBMCs (AllCells) that were activated by PHA (5 μg/mL) for 4 days before transplantation (NSG-hu mice).

    Techniques: Infection, Mouse Assay, Neutralization, Activity Assay, Luciferase, Expressing, Recombinant, Injection, Purification, Enzyme-linked Immunosorbent Assay

    RMD does not induce global activation of immune cell subsets. PBMCs isolated from four HIV-infected patients on suppressive cART were treated with a 4-hour pulse of RMD or continuously with vehicle control (DMSO), VOR, or PMA+ionomycin and stained for surface markers 48 hours after the treatment initiation. Fractions of CD69−, CD25−, and HLA-DR-positive cells in subsets of CD4+ T cells, CD8+ T cells, and CD19+ B cells were analyzed by flow cytometry as described in Materials and Methods . Each symbol represents one donor.

    Journal: PLoS Pathogens

    Article Title: Histone Deacetylase Inhibitor Romidepsin Induces HIV Expression in CD4 T Cells from Patients on Suppressive Antiretroviral Therapy at Concentrations Achieved by Clinical Dosing

    doi: 10.1371/journal.ppat.1004071

    Figure Lengend Snippet: RMD does not induce global activation of immune cell subsets. PBMCs isolated from four HIV-infected patients on suppressive cART were treated with a 4-hour pulse of RMD or continuously with vehicle control (DMSO), VOR, or PMA+ionomycin and stained for surface markers 48 hours after the treatment initiation. Fractions of CD69−, CD25−, and HLA-DR-positive cells in subsets of CD4+ T cells, CD8+ T cells, and CD19+ B cells were analyzed by flow cytometry as described in Materials and Methods . Each symbol represents one donor.

    Article Snippet: In vitro model of HIV latency Total peripheral blood mononuclear cells (PBMCs) were obtained from healthy HIV-negative donors by leukapheresis (AllCells, Inc, Emeryville, CA).

    Techniques: Activation Assay, Isolation, Infection, Staining, Flow Cytometry, Cytometry