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TaKaRa human pbmcs
The differences of mRNA expression of IL-38, IL-36R, IL-13, IL-10 and FoxP3 between asthmatic patients and controls. <t>PBMC</t> was purified and total RNA was extracted, reverse transcribed and analyzed by real time <t>PCR.</t> Results are presented as box and whisker plots with median (interquartile range). Statistical significances were indicated by * p
Human Pbmcs, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Aberrant Expression of Novel Cytokine IL-38 and Regulatory T Lymphocytes in Childhood Asthma

Journal: Molecules

doi: 10.3390/molecules21070933

The differences of mRNA expression of IL-38, IL-36R, IL-13, IL-10 and FoxP3 between asthmatic patients and controls. PBMC was purified and total RNA was extracted, reverse transcribed and analyzed by real time PCR. Results are presented as box and whisker plots with median (interquartile range). Statistical significances were indicated by * p
Figure Legend Snippet: The differences of mRNA expression of IL-38, IL-36R, IL-13, IL-10 and FoxP3 between asthmatic patients and controls. PBMC was purified and total RNA was extracted, reverse transcribed and analyzed by real time PCR. Results are presented as box and whisker plots with median (interquartile range). Statistical significances were indicated by * p

Techniques Used: Expressing, Purification, Real-time Polymerase Chain Reaction, Whisker Assay

Related Articles

Sequencing:

Article Title: CCR6 Is a Prognostic Marker for Overall Survival in Patients with Colorectal Cancer, and Its Overexpression Enhances Metastasis In Vivo
Article Snippet: .. Briefly, the 1125-bp coding sequence of human CCR6 (NM_004367) was amplified from the cDNA template of human PBMCs and subcloned into the XhoI and BamHI sites of the pLVX-IRES-ZsGreen1 vector (Clontech Laboratories, USA). .. The lentiviral shuttle vector pLVXshRNA2 (Clontech Laboratories, USA) was used to express shRNAs from the U6 promoter.

Real-time Polymerase Chain Reaction:

Article Title: Aberrant Expression of Novel Cytokine IL-38 and Regulatory T Lymphocytes in Childhood Asthma
Article Snippet: .. Real time PCR Total RNA of human PBMCs (1 × 106 cells) were extracted and transcribed into first-strand complementary DNA using PrimeScript™ RT Master Mix (Takara Bio Inc., Shiga, Japan). .. The quantitative expression of target genes was measured using FastStart Universal SYBR Green Master (ROX) (Roche Applied Science, Basel, Switzerland) with corresponding primers ( ).

Amplification:

Article Title: CCR6 Is a Prognostic Marker for Overall Survival in Patients with Colorectal Cancer, and Its Overexpression Enhances Metastasis In Vivo
Article Snippet: .. Briefly, the 1125-bp coding sequence of human CCR6 (NM_004367) was amplified from the cDNA template of human PBMCs and subcloned into the XhoI and BamHI sites of the pLVX-IRES-ZsGreen1 vector (Clontech Laboratories, USA). .. The lentiviral shuttle vector pLVXshRNA2 (Clontech Laboratories, USA) was used to express shRNAs from the U6 promoter.

Article Title: Molecular Basis of Evolutionary Loss of the α1,3-Galactosyltransferase Gene in Higher Primates *
Article Snippet: .. Using Trizol™ reagent (Invitrogen) according to the manufacturer’s instructions, total RNA was extracted from rhesus PBMC and spleen samples, from human PBMC and placenta (provided in the CLONTECH Marathon™ amplification kit), and from orangutan PBMC. .. After treatment with DNase I (Ambion Inc., Austin, TX), poly(A)+ RNA was separated from total RNA using the Dynabeads™ mRNA purification Kit (Dynal, Oslo, Norway) in accordance with the protocol provided.

Plasmid Preparation:

Article Title: CCR6 Is a Prognostic Marker for Overall Survival in Patients with Colorectal Cancer, and Its Overexpression Enhances Metastasis In Vivo
Article Snippet: .. Briefly, the 1125-bp coding sequence of human CCR6 (NM_004367) was amplified from the cDNA template of human PBMCs and subcloned into the XhoI and BamHI sites of the pLVX-IRES-ZsGreen1 vector (Clontech Laboratories, USA). .. The lentiviral shuttle vector pLVXshRNA2 (Clontech Laboratories, USA) was used to express shRNAs from the U6 promoter.

Derivative Assay:

Article Title: Down-modulation of primate lentiviral receptors by Nef proteins of simian immunodeficiency virus (SIV) of chimpanzees (SIVcpz) and related SIVs: implication for the evolutionary event at the emergence of SIVcpz
Article Snippet: .. The ORFs of human CCR2B and CXCR6 were obtained by reverse transcription-PCR using cDNA derived from human PBMCs as the template, PrimeSTAR GXL DNA polymerase (TaKaRa) and the following primers: CCR2B-fwd, 5′-TTTTTTTTTTGGTACCGCCACCATGCTGTCCACATCTCGTTC-3′; CCR2B-rev, 5′-TATATATATAAAGCTTTTATAAACCAGCCGAGACTTCC-3′; CXCR6-fwd, 5′-TTTTTTTTTTGGTACCGCCACCATGGCAGAGCATGATTACCATG-3′; and CXCR6-rev, 5′-TATATATATAAAGCTTCTATAACTGGAACATGCTGGTG-3′. .. The obtained DNA fragments were digested with Kpn I and Hin dIII, and then inserted into the Kpn I/ Hin dIII site of pCMV-SPORT6 (Life Technologies).

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    TaKaRa human pbmcs
    The differences of mRNA expression of IL-38, IL-36R, IL-13, IL-10 and FoxP3 between asthmatic patients and controls. <t>PBMC</t> was purified and total RNA was extracted, reverse transcribed and analyzed by real time <t>PCR.</t> Results are presented as box and whisker plots with median (interquartile range). Statistical significances were indicated by * p
    Human Pbmcs, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pbmcs/product/TaKaRa
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human pbmcs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    TaKaRa human peripheral blood mononuclear cells pbmcs
    The differences of mRNA expression of IL-38, IL-36R, IL-13, IL-10 and FoxP3 between asthmatic patients and controls. <t>PBMC</t> was purified and total RNA was extracted, reverse transcribed and analyzed by real time <t>PCR.</t> Results are presented as box and whisker plots with median (interquartile range). Statistical significances were indicated by * p
    Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human peripheral blood mononuclear cells pbmcs/product/TaKaRa
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    The differences of mRNA expression of IL-38, IL-36R, IL-13, IL-10 and FoxP3 between asthmatic patients and controls. PBMC was purified and total RNA was extracted, reverse transcribed and analyzed by real time PCR. Results are presented as box and whisker plots with median (interquartile range). Statistical significances were indicated by * p

    Journal: Molecules

    Article Title: Aberrant Expression of Novel Cytokine IL-38 and Regulatory T Lymphocytes in Childhood Asthma

    doi: 10.3390/molecules21070933

    Figure Lengend Snippet: The differences of mRNA expression of IL-38, IL-36R, IL-13, IL-10 and FoxP3 between asthmatic patients and controls. PBMC was purified and total RNA was extracted, reverse transcribed and analyzed by real time PCR. Results are presented as box and whisker plots with median (interquartile range). Statistical significances were indicated by * p

    Article Snippet: Real time PCR Total RNA of human PBMCs (1 × 106 cells) were extracted and transcribed into first-strand complementary DNA using PrimeScript™ RT Master Mix (Takara Bio Inc., Shiga, Japan).

    Techniques: Expressing, Purification, Real-time Polymerase Chain Reaction, Whisker Assay