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Reachbio human pbmcs
(A) Transcriptome analysis of <t>KSHV</t> during de novo infection of human <t>PBMCs.</t> Total RNAs extracted from de novo -infected PBMCs harvested at 4 h, 24 h, 48 h, 72 h, and 120 h postinfection were subjected to cDNA library preparation using a TrueSeq RNA-seq
Human Pbmcs, supplied by Reachbio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92/100 stars

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1) Product Images from "Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells"

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells

Journal: Journal of Virology

doi: 10.1128/JVI.02507-14

(A) Transcriptome analysis of KSHV during de novo infection of human PBMCs. Total RNAs extracted from de novo -infected PBMCs harvested at 4 h, 24 h, 48 h, 72 h, and 120 h postinfection were subjected to cDNA library preparation using a TrueSeq RNA-seq
Figure Legend Snippet: (A) Transcriptome analysis of KSHV during de novo infection of human PBMCs. Total RNAs extracted from de novo -infected PBMCs harvested at 4 h, 24 h, 48 h, 72 h, and 120 h postinfection were subjected to cDNA library preparation using a TrueSeq RNA-seq

Techniques Used: Infection, cDNA Library Assay, RNA Sequencing Assay

Transcriptome analysis of KSHV in de novo -infected PBMCs, TIVE cells, and CD14 + cells. RPKM values, calculated based the number of reads for each gene, were used for analyzing relative expression of KSHV genes as heat maps. Hierarchal clustering of genes
Figure Legend Snippet: Transcriptome analysis of KSHV in de novo -infected PBMCs, TIVE cells, and CD14 + cells. RPKM values, calculated based the number of reads for each gene, were used for analyzing relative expression of KSHV genes as heat maps. Hierarchal clustering of genes

Techniques Used: Infection, Expressing

Viral genes transcribed at 4 hpi and 24 hpi, identified by a nascent RNA capture approach. Human PBMCs infected with KSHV virions were incubated with EdU (alkyne) at 4 hpi and 24 hpi to label the newly transcribing RNA. Total RNA extracted from these
Figure Legend Snippet: Viral genes transcribed at 4 hpi and 24 hpi, identified by a nascent RNA capture approach. Human PBMCs infected with KSHV virions were incubated with EdU (alkyne) at 4 hpi and 24 hpi to label the newly transcribing RNA. Total RNA extracted from these

Techniques Used: Infection, Incubation

KSHV viral transcripts are abundantly present during the primary infection of human PBMCs, CD14+ , and TIVE cells.
Figure Legend Snippet: KSHV viral transcripts are abundantly present during the primary infection of human PBMCs, CD14+ , and TIVE cells.

Techniques Used: Infection

KSHV genome copies exponentially increase after infection. (A) Approximately 8 × 10 7 human PBMCs were infected with KSHV isolated from reactivated TRExBCBL1-RTA, with a multiplicity of infection (MOI) of 10. De novo -infected PBMCs were harvested
Figure Legend Snippet: KSHV genome copies exponentially increase after infection. (A) Approximately 8 × 10 7 human PBMCs were infected with KSHV isolated from reactivated TRExBCBL1-RTA, with a multiplicity of infection (MOI) of 10. De novo -infected PBMCs were harvested

Techniques Used: Infection, Isolation

2) Product Images from "Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells"

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells

Journal: Journal of Virology

doi: 10.1128/JVI.02507-14

(A) Transcriptome analysis of KSHV during de novo infection of human PBMCs. Total RNAs extracted from de novo -infected PBMCs harvested at 4 h, 24 h, 48 h, 72 h, and 120 h postinfection were subjected to cDNA library preparation using a TrueSeq RNA-seq
Figure Legend Snippet: (A) Transcriptome analysis of KSHV during de novo infection of human PBMCs. Total RNAs extracted from de novo -infected PBMCs harvested at 4 h, 24 h, 48 h, 72 h, and 120 h postinfection were subjected to cDNA library preparation using a TrueSeq RNA-seq

Techniques Used: Infection, cDNA Library Assay, RNA Sequencing Assay

Viral genes transcribed at 4 hpi and 24 hpi, identified by a nascent RNA capture approach. Human PBMCs infected with KSHV virions were incubated with EdU (alkyne) at 4 hpi and 24 hpi to label the newly transcribing RNA. Total RNA extracted from these
Figure Legend Snippet: Viral genes transcribed at 4 hpi and 24 hpi, identified by a nascent RNA capture approach. Human PBMCs infected with KSHV virions were incubated with EdU (alkyne) at 4 hpi and 24 hpi to label the newly transcribing RNA. Total RNA extracted from these

Techniques Used: Infection, Incubation

Real-time qPCR validation of de novo -infected PBMCs. Real-time qPCR analysis was performed on the cDNA prepared from total RNA harvested at 4 h, 24 h, 48 h, 96 h, and 120 h postinfection of human PBMCs. A 96-well format qPCR plate representing each of
Figure Legend Snippet: Real-time qPCR validation of de novo -infected PBMCs. Real-time qPCR analysis was performed on the cDNA prepared from total RNA harvested at 4 h, 24 h, 48 h, 96 h, and 120 h postinfection of human PBMCs. A 96-well format qPCR plate representing each of

Techniques Used: Real-time Polymerase Chain Reaction, Infection

3) Product Images from "Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells"

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells

Journal: Journal of Virology

doi: 10.1128/JVI.02507-14

Transcriptome analysis of KSHV in de novo -infected PBMCs, TIVE cells, and CD14 + cells. RPKM values, calculated based the number of reads for each gene, were used for analyzing relative expression of KSHV genes as heat maps. Hierarchal clustering of genes
Figure Legend Snippet: Transcriptome analysis of KSHV in de novo -infected PBMCs, TIVE cells, and CD14 + cells. RPKM values, calculated based the number of reads for each gene, were used for analyzing relative expression of KSHV genes as heat maps. Hierarchal clustering of genes

Techniques Used: Infection, Expressing

KSHV viral transcripts are abundantly present during the primary infection of human PBMCs, CD14+ , and TIVE cells.
Figure Legend Snippet: KSHV viral transcripts are abundantly present during the primary infection of human PBMCs, CD14+ , and TIVE cells.

Techniques Used: Infection

Related Articles

Amplification:

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells
Article Snippet: .. Treating the cells with a late gene expression inhibitor, PAA, led to a significantly reduced number of viral genome copies, further substantiating the involvement of lytic DNA replication in genome copy amplification in human PBMCs. .. Discriminating whether the transcripts detected at 4 hpi were due to the virion-transduced mRNA or active transcription, RNA-seq analysis of the newly synthesized transcripts at 4 hpi of human PBMCs showed transcription of only a limited number of genes during primary infection.

RNA Sequencing Assay:

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells
Article Snippet: .. Discriminating whether the transcripts detected at 4 hpi were due to the virion-transduced mRNA or active transcription, RNA-seq analysis of the newly synthesized transcripts at 4 hpi of human PBMCs showed transcription of only a limited number of genes during primary infection. .. However, a large number of viral transcripts are detected at 4 hpi as well as in the virion particles, which confirms that those transcripts are introduced into the infected cells along with the virions.

Synthesized:

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells
Article Snippet: .. Discriminating whether the transcripts detected at 4 hpi were due to the virion-transduced mRNA or active transcription, RNA-seq analysis of the newly synthesized transcripts at 4 hpi of human PBMCs showed transcription of only a limited number of genes during primary infection. .. However, a large number of viral transcripts are detected at 4 hpi as well as in the virion particles, which confirms that those transcripts are introduced into the infected cells along with the virions.

Infection:

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells
Article Snippet: .. To determine the profiles of the viral transcripts expressed during the primary infection of B and endothelial cells (natural target cells of KSHV), purified virions were used for the de novo infection of human PBMCs, CD14+ monocytes, and endothelial (TIVE) cells. .. An RNA-seq analysis was performed on total RNA extracted from these infected cells at different time points (4, 24, 48, 72, and 120 hpi).

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells
Article Snippet: .. We normalized the number of virions used for the infection of human PBMCs by extracting the virion DNA and quantifying it in a real-time qPCR assay. .. Cells were collected at 4, 24, 48, 72, 96, and 120 hpi and analyzed for viral gene expression and the number of KSHV genome copies during early infection.

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells
Article Snippet: .. Overall, these data suggest that ORF59 is required for replicating the DNA and amplifying the viral genome during primary infection of human PBMCs. .. KSHV infection is a complex multistep process involving the regulation of various cellular pathways.

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells
Article Snippet: .. Discriminating whether the transcripts detected at 4 hpi were due to the virion-transduced mRNA or active transcription, RNA-seq analysis of the newly synthesized transcripts at 4 hpi of human PBMCs showed transcription of only a limited number of genes during primary infection. .. However, a large number of viral transcripts are detected at 4 hpi as well as in the virion particles, which confirms that those transcripts are introduced into the infected cells along with the virions.

Purification:

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells
Article Snippet: .. To determine the profiles of the viral transcripts expressed during the primary infection of B and endothelial cells (natural target cells of KSHV), purified virions were used for the de novo infection of human PBMCs, CD14+ monocytes, and endothelial (TIVE) cells. .. An RNA-seq analysis was performed on total RNA extracted from these infected cells at different time points (4, 24, 48, 72, and 120 hpi).

Real-time Polymerase Chain Reaction:

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells
Article Snippet: .. We normalized the number of virions used for the infection of human PBMCs by extracting the virion DNA and quantifying it in a real-time qPCR assay. .. Cells were collected at 4, 24, 48, 72, 96, and 120 hpi and analyzed for viral gene expression and the number of KSHV genome copies during early infection.

other:

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells
Article Snippet: However, the human PBMCs and TIVE cells showed detectable levels of PAN RNA and ORF58/59 transcripts along with LANA after 48 hpi ( to and ).

Cell Culture:

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells
Article Snippet: .. Human PBMCs (ReachBio, WA) were cultured in RPMI 1640 medium (HyClone) supplemented with 10% FBS, 2 mM l -glutamine, and penicillin-streptomycin (5 U/ml and 5 μg/ml, respectively). .. Human CD14+ cells were isolated from cord blood received from the Colorado Cord Blood Bank (University of Colorado).

Expressing:

Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells
Article Snippet: .. Treating the cells with a late gene expression inhibitor, PAA, led to a significantly reduced number of viral genome copies, further substantiating the involvement of lytic DNA replication in genome copy amplification in human PBMCs. .. Discriminating whether the transcripts detected at 4 hpi were due to the virion-transduced mRNA or active transcription, RNA-seq analysis of the newly synthesized transcripts at 4 hpi of human PBMCs showed transcription of only a limited number of genes during primary infection.

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    Reachbio human pbmcs
    (A) Transcriptome analysis of <t>KSHV</t> during de novo infection of human <t>PBMCs.</t> Total RNAs extracted from de novo -infected PBMCs harvested at 4 h, 24 h, 48 h, 72 h, and 120 h postinfection were subjected to cDNA library preparation using a TrueSeq RNA-seq
    Human Pbmcs, supplied by Reachbio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pbmcs/product/Reachbio
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human pbmcs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    85
    Reachbio treatments frozen human peripheral blood mononuclear cells pbmcs
    Expression of the IFI16 and AIM2 proteins is cell type dependent. ( A ) Total cell extracts containing equal amounts of proteins from peripheral blood mononuclear cells (PBMNC), purified monocytes <t>(CD14</t> + ), or B lymphocytes (CD19 + ) were analyzed by immunoblotting using antibodies specific to the indicated proteins. The arrowheads indicate different forms of the AIM2 (53 and 49 kDa) and IFI16 (A, B, and C forms) proteins. ( B ) Total RNA was extracted from total PBMNCs, purified CD14 + or CD19 + cells. Steady state levels of the IFI16 or AIM2 mRNA were analyzed by quantitative TaqMan real-time PCR. The ratio of the test gene to actin mRNA was calculated in units (one unit being the ratio of the test gene to actin mRNA). Results are mean values of triplicate experiments and error bars represent standard deviation. ( C ) Total RNA as described in panel ( B ) was also analyzed by the quantitative TaqMan real-time PCR for the IFN-β mRNA. Again, the ratio of the test gene to actin mRNA was calculated in units. Results are mean values of triplicate experiments and error bars represent standard deviation.
    Treatments Frozen Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by Reachbio, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/treatments frozen human peripheral blood mononuclear cells pbmcs/product/Reachbio
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    treatments frozen human peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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    (A) Transcriptome analysis of KSHV during de novo infection of human PBMCs. Total RNAs extracted from de novo -infected PBMCs harvested at 4 h, 24 h, 48 h, 72 h, and 120 h postinfection were subjected to cDNA library preparation using a TrueSeq RNA-seq

    Journal: Journal of Virology

    Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells

    doi: 10.1128/JVI.02507-14

    Figure Lengend Snippet: (A) Transcriptome analysis of KSHV during de novo infection of human PBMCs. Total RNAs extracted from de novo -infected PBMCs harvested at 4 h, 24 h, 48 h, 72 h, and 120 h postinfection were subjected to cDNA library preparation using a TrueSeq RNA-seq

    Article Snippet: To determine the profiles of the viral transcripts expressed during the primary infection of B and endothelial cells (natural target cells of KSHV), purified virions were used for the de novo infection of human PBMCs, CD14+ monocytes, and endothelial (TIVE) cells.

    Techniques: Infection, cDNA Library Assay, RNA Sequencing Assay

    Transcriptome analysis of KSHV in de novo -infected PBMCs, TIVE cells, and CD14 + cells. RPKM values, calculated based the number of reads for each gene, were used for analyzing relative expression of KSHV genes as heat maps. Hierarchal clustering of genes

    Journal: Journal of Virology

    Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells

    doi: 10.1128/JVI.02507-14

    Figure Lengend Snippet: Transcriptome analysis of KSHV in de novo -infected PBMCs, TIVE cells, and CD14 + cells. RPKM values, calculated based the number of reads for each gene, were used for analyzing relative expression of KSHV genes as heat maps. Hierarchal clustering of genes

    Article Snippet: To determine the profiles of the viral transcripts expressed during the primary infection of B and endothelial cells (natural target cells of KSHV), purified virions were used for the de novo infection of human PBMCs, CD14+ monocytes, and endothelial (TIVE) cells.

    Techniques: Infection, Expressing

    Viral genes transcribed at 4 hpi and 24 hpi, identified by a nascent RNA capture approach. Human PBMCs infected with KSHV virions were incubated with EdU (alkyne) at 4 hpi and 24 hpi to label the newly transcribing RNA. Total RNA extracted from these

    Journal: Journal of Virology

    Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells

    doi: 10.1128/JVI.02507-14

    Figure Lengend Snippet: Viral genes transcribed at 4 hpi and 24 hpi, identified by a nascent RNA capture approach. Human PBMCs infected with KSHV virions were incubated with EdU (alkyne) at 4 hpi and 24 hpi to label the newly transcribing RNA. Total RNA extracted from these

    Article Snippet: To determine the profiles of the viral transcripts expressed during the primary infection of B and endothelial cells (natural target cells of KSHV), purified virions were used for the de novo infection of human PBMCs, CD14+ monocytes, and endothelial (TIVE) cells.

    Techniques: Infection, Incubation

    KSHV viral transcripts are abundantly present during the primary infection of human PBMCs, CD14+ , and TIVE cells.

    Journal: Journal of Virology

    Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells

    doi: 10.1128/JVI.02507-14

    Figure Lengend Snippet: KSHV viral transcripts are abundantly present during the primary infection of human PBMCs, CD14+ , and TIVE cells.

    Article Snippet: To determine the profiles of the viral transcripts expressed during the primary infection of B and endothelial cells (natural target cells of KSHV), purified virions were used for the de novo infection of human PBMCs, CD14+ monocytes, and endothelial (TIVE) cells.

    Techniques: Infection

    KSHV genome copies exponentially increase after infection. (A) Approximately 8 × 10 7 human PBMCs were infected with KSHV isolated from reactivated TRExBCBL1-RTA, with a multiplicity of infection (MOI) of 10. De novo -infected PBMCs were harvested

    Journal: Journal of Virology

    Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells

    doi: 10.1128/JVI.02507-14

    Figure Lengend Snippet: KSHV genome copies exponentially increase after infection. (A) Approximately 8 × 10 7 human PBMCs were infected with KSHV isolated from reactivated TRExBCBL1-RTA, with a multiplicity of infection (MOI) of 10. De novo -infected PBMCs were harvested

    Article Snippet: To determine the profiles of the viral transcripts expressed during the primary infection of B and endothelial cells (natural target cells of KSHV), purified virions were used for the de novo infection of human PBMCs, CD14+ monocytes, and endothelial (TIVE) cells.

    Techniques: Infection, Isolation

    Real-time qPCR validation of de novo -infected PBMCs. Real-time qPCR analysis was performed on the cDNA prepared from total RNA harvested at 4 h, 24 h, 48 h, 96 h, and 120 h postinfection of human PBMCs. A 96-well format qPCR plate representing each of

    Journal: Journal of Virology

    Article Title: Transcriptome Analysis of Kaposi's Sarcoma-Associated Herpesvirus during De Novo Primary Infection of Human B and Endothelial Cells

    doi: 10.1128/JVI.02507-14

    Figure Lengend Snippet: Real-time qPCR validation of de novo -infected PBMCs. Real-time qPCR analysis was performed on the cDNA prepared from total RNA harvested at 4 h, 24 h, 48 h, 96 h, and 120 h postinfection of human PBMCs. A 96-well format qPCR plate representing each of

    Article Snippet: Discriminating whether the transcripts detected at 4 hpi were due to the virion-transduced mRNA or active transcription, RNA-seq analysis of the newly synthesized transcripts at 4 hpi of human PBMCs showed transcription of only a limited number of genes during primary infection.

    Techniques: Real-time Polymerase Chain Reaction, Infection

    Expression of the IFI16 and AIM2 proteins is cell type dependent. ( A ) Total cell extracts containing equal amounts of proteins from peripheral blood mononuclear cells (PBMNC), purified monocytes (CD14 + ), or B lymphocytes (CD19 + ) were analyzed by immunoblotting using antibodies specific to the indicated proteins. The arrowheads indicate different forms of the AIM2 (53 and 49 kDa) and IFI16 (A, B, and C forms) proteins. ( B ) Total RNA was extracted from total PBMNCs, purified CD14 + or CD19 + cells. Steady state levels of the IFI16 or AIM2 mRNA were analyzed by quantitative TaqMan real-time PCR. The ratio of the test gene to actin mRNA was calculated in units (one unit being the ratio of the test gene to actin mRNA). Results are mean values of triplicate experiments and error bars represent standard deviation. ( C ) Total RNA as described in panel ( B ) was also analyzed by the quantitative TaqMan real-time PCR for the IFN-β mRNA. Again, the ratio of the test gene to actin mRNA was calculated in units. Results are mean values of triplicate experiments and error bars represent standard deviation.

    Journal: PLoS ONE

    Article Title: IFI16 Protein Mediates the Anti-inflammatory Actions of the Type-I Interferons through Suppression of Activation of Caspase-1 by Inflammasomes

    doi: 10.1371/journal.pone.0027040

    Figure Lengend Snippet: Expression of the IFI16 and AIM2 proteins is cell type dependent. ( A ) Total cell extracts containing equal amounts of proteins from peripheral blood mononuclear cells (PBMNC), purified monocytes (CD14 + ), or B lymphocytes (CD19 + ) were analyzed by immunoblotting using antibodies specific to the indicated proteins. The arrowheads indicate different forms of the AIM2 (53 and 49 kDa) and IFI16 (A, B, and C forms) proteins. ( B ) Total RNA was extracted from total PBMNCs, purified CD14 + or CD19 + cells. Steady state levels of the IFI16 or AIM2 mRNA were analyzed by quantitative TaqMan real-time PCR. The ratio of the test gene to actin mRNA was calculated in units (one unit being the ratio of the test gene to actin mRNA). Results are mean values of triplicate experiments and error bars represent standard deviation. ( C ) Total RNA as described in panel ( B ) was also analyzed by the quantitative TaqMan real-time PCR for the IFN-β mRNA. Again, the ratio of the test gene to actin mRNA was calculated in units. Results are mean values of triplicate experiments and error bars represent standard deviation.

    Article Snippet: Cells and Treatments Frozen human peripheral blood mononuclear cells (PBMCs), purified CD14+ monocytes and CD19+ B cells were purchased from ReachBio (Seattle, WA).

    Techniques: Expressing, Purification, Real-time Polymerase Chain Reaction, Standard Deviation