Structured Review

Kurabo industries human pbmcs
AZT induces ASK1 and promotes the antiviral activity of A3G. ( a , b ) <t>PBMC</t> or <t>H9</t> cells were treated with the indicated agents (0–10 μM) for 24 h. The expression of ASK1 was confirmed by western blotting. APV, amprenavir; ATV, atazanavir; AZT, zidovudine; DRV, darunavir; IDV, indinavir; LPV, lopinavir; NFV, nelfinavir; NVP, nevirapine; SQV, saquinavir; 3TC, lamivudine. ( c ) H9 cells were transfected with control siRNA or ASK1-targeted siRNA for 48 h before infection of HIV-1. At 2 days after infection, cells were washed and additionally cultured for 24 h in the presence or absence of AZT (10 μM). Cells and supernatants were then harvested and analysed by western blotting against the indicated antibodies. We used AZT-resistant virus to avoid the effects of AZT carry-over from the culture supernatant of producer cells during the reverse transcription step in target cells. ( d ) TZM-bl reporter cells were infected with harvested and normalized virus to measure viral infectivity ( n =3, mean±s.d.). Full images for all western blots analysis are shown in Supplementary Fig. 6 .
Human Pbmcs, supplied by Kurabo industries, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human pbmcs/product/Kurabo industries
Average 93 stars, based on 9 article reviews
Price from $9.99 to $1999.99
human pbmcs - by Bioz Stars, 2020-09
93/100 stars

Images

1) Product Images from "ASK1 restores the antiviral activity of APOBEC3G by disrupting HIV-1 Vif-mediated counteraction"

Article Title: ASK1 restores the antiviral activity of APOBEC3G by disrupting HIV-1 Vif-mediated counteraction

Journal: Nature Communications

doi: 10.1038/ncomms7945

AZT induces ASK1 and promotes the antiviral activity of A3G. ( a , b ) PBMC or H9 cells were treated with the indicated agents (0–10 μM) for 24 h. The expression of ASK1 was confirmed by western blotting. APV, amprenavir; ATV, atazanavir; AZT, zidovudine; DRV, darunavir; IDV, indinavir; LPV, lopinavir; NFV, nelfinavir; NVP, nevirapine; SQV, saquinavir; 3TC, lamivudine. ( c ) H9 cells were transfected with control siRNA or ASK1-targeted siRNA for 48 h before infection of HIV-1. At 2 days after infection, cells were washed and additionally cultured for 24 h in the presence or absence of AZT (10 μM). Cells and supernatants were then harvested and analysed by western blotting against the indicated antibodies. We used AZT-resistant virus to avoid the effects of AZT carry-over from the culture supernatant of producer cells during the reverse transcription step in target cells. ( d ) TZM-bl reporter cells were infected with harvested and normalized virus to measure viral infectivity ( n =3, mean±s.d.). Full images for all western blots analysis are shown in Supplementary Fig. 6 .
Figure Legend Snippet: AZT induces ASK1 and promotes the antiviral activity of A3G. ( a , b ) PBMC or H9 cells were treated with the indicated agents (0–10 μM) for 24 h. The expression of ASK1 was confirmed by western blotting. APV, amprenavir; ATV, atazanavir; AZT, zidovudine; DRV, darunavir; IDV, indinavir; LPV, lopinavir; NFV, nelfinavir; NVP, nevirapine; SQV, saquinavir; 3TC, lamivudine. ( c ) H9 cells were transfected with control siRNA or ASK1-targeted siRNA for 48 h before infection of HIV-1. At 2 days after infection, cells were washed and additionally cultured for 24 h in the presence or absence of AZT (10 μM). Cells and supernatants were then harvested and analysed by western blotting against the indicated antibodies. We used AZT-resistant virus to avoid the effects of AZT carry-over from the culture supernatant of producer cells during the reverse transcription step in target cells. ( d ) TZM-bl reporter cells were infected with harvested and normalized virus to measure viral infectivity ( n =3, mean±s.d.). Full images for all western blots analysis are shown in Supplementary Fig. 6 .

Techniques Used: Activity Assay, Expressing, Western Blot, Transfection, Infection, Cell Culture

Related Articles

Cell Culture:

Article Title: ASK1 restores the antiviral activity of APOBEC3G by disrupting HIV-1 Vif-mediated counteraction
Article Snippet: .. CEM, CEMSS, H9, M8166 (NIH AIDS Reagent Program) and human PBMCs (purchased from Kurabo, Osaka, Japan) were cultured in RPMI containing 10% fetal bovine serum. .. To generate CEM-TetON-ASK1 or CEMSS-TetON-ASK1 cells, parental cells were co-infected with VSV-G-pseudotyped retroviral vectors expressing pRetroX-TRE3G-ASK1 and pRetroX-Tet3G (Clontech) and selected with G418 and puromycin.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Kurabo industries human pbmcs
    AZT induces ASK1 and promotes the antiviral activity of A3G. ( a , b ) <t>PBMC</t> or <t>H9</t> cells were treated with the indicated agents (0–10 μM) for 24 h. The expression of ASK1 was confirmed by western blotting. APV, amprenavir; ATV, atazanavir; AZT, zidovudine; DRV, darunavir; IDV, indinavir; LPV, lopinavir; NFV, nelfinavir; NVP, nevirapine; SQV, saquinavir; 3TC, lamivudine. ( c ) H9 cells were transfected with control siRNA or ASK1-targeted siRNA for 48 h before infection of HIV-1. At 2 days after infection, cells were washed and additionally cultured for 24 h in the presence or absence of AZT (10 μM). Cells and supernatants were then harvested and analysed by western blotting against the indicated antibodies. We used AZT-resistant virus to avoid the effects of AZT carry-over from the culture supernatant of producer cells during the reverse transcription step in target cells. ( d ) TZM-bl reporter cells were infected with harvested and normalized virus to measure viral infectivity ( n =3, mean±s.d.). Full images for all western blots analysis are shown in Supplementary Fig. 6 .
    Human Pbmcs, supplied by Kurabo industries, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pbmcs/product/Kurabo industries
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    human pbmcs - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    AZT induces ASK1 and promotes the antiviral activity of A3G. ( a , b ) PBMC or H9 cells were treated with the indicated agents (0–10 μM) for 24 h. The expression of ASK1 was confirmed by western blotting. APV, amprenavir; ATV, atazanavir; AZT, zidovudine; DRV, darunavir; IDV, indinavir; LPV, lopinavir; NFV, nelfinavir; NVP, nevirapine; SQV, saquinavir; 3TC, lamivudine. ( c ) H9 cells were transfected with control siRNA or ASK1-targeted siRNA for 48 h before infection of HIV-1. At 2 days after infection, cells were washed and additionally cultured for 24 h in the presence or absence of AZT (10 μM). Cells and supernatants were then harvested and analysed by western blotting against the indicated antibodies. We used AZT-resistant virus to avoid the effects of AZT carry-over from the culture supernatant of producer cells during the reverse transcription step in target cells. ( d ) TZM-bl reporter cells were infected with harvested and normalized virus to measure viral infectivity ( n =3, mean±s.d.). Full images for all western blots analysis are shown in Supplementary Fig. 6 .

    Journal: Nature Communications

    Article Title: ASK1 restores the antiviral activity of APOBEC3G by disrupting HIV-1 Vif-mediated counteraction

    doi: 10.1038/ncomms7945

    Figure Lengend Snippet: AZT induces ASK1 and promotes the antiviral activity of A3G. ( a , b ) PBMC or H9 cells were treated with the indicated agents (0–10 μM) for 24 h. The expression of ASK1 was confirmed by western blotting. APV, amprenavir; ATV, atazanavir; AZT, zidovudine; DRV, darunavir; IDV, indinavir; LPV, lopinavir; NFV, nelfinavir; NVP, nevirapine; SQV, saquinavir; 3TC, lamivudine. ( c ) H9 cells were transfected with control siRNA or ASK1-targeted siRNA for 48 h before infection of HIV-1. At 2 days after infection, cells were washed and additionally cultured for 24 h in the presence or absence of AZT (10 μM). Cells and supernatants were then harvested and analysed by western blotting against the indicated antibodies. We used AZT-resistant virus to avoid the effects of AZT carry-over from the culture supernatant of producer cells during the reverse transcription step in target cells. ( d ) TZM-bl reporter cells were infected with harvested and normalized virus to measure viral infectivity ( n =3, mean±s.d.). Full images for all western blots analysis are shown in Supplementary Fig. 6 .

    Article Snippet: CEM, CEMSS, H9, M8166 (NIH AIDS Reagent Program) and human PBMCs (purchased from Kurabo, Osaka, Japan) were cultured in RPMI containing 10% fetal bovine serum.

    Techniques: Activity Assay, Expressing, Western Blot, Transfection, Infection, Cell Culture