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Cellular Technology Ltd human pbmcs
The evaluation of the immunostimulatory effects of <t>NIPAM-hemin.</t> Abbreviations: NIPAM, N -isopropylacrylamide; hemin, ferriprotoporphyrin IX chloride; IFN-γ, interferon-γ; IL, interleukin; NK, natural killer; <t>PBMCs,</t> peripheral blood mononuclear cells; ELISA, enzyme-linked immunosorbent assay; TLRs, toll-like receptors; SEAP, secreted embryonic alkaline phosphatase.
Human Pbmcs, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Synthesis of a hemin-containing copolymer as a novel immunostimulator that induces IFN-gamma production

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S166259

The evaluation of the immunostimulatory effects of NIPAM-hemin. Abbreviations: NIPAM, N -isopropylacrylamide; hemin, ferriprotoporphyrin IX chloride; IFN-γ, interferon-γ; IL, interleukin; NK, natural killer; PBMCs, peripheral blood mononuclear cells; ELISA, enzyme-linked immunosorbent assay; TLRs, toll-like receptors; SEAP, secreted embryonic alkaline phosphatase.
Figure Legend Snippet: The evaluation of the immunostimulatory effects of NIPAM-hemin. Abbreviations: NIPAM, N -isopropylacrylamide; hemin, ferriprotoporphyrin IX chloride; IFN-γ, interferon-γ; IL, interleukin; NK, natural killer; PBMCs, peripheral blood mononuclear cells; ELISA, enzyme-linked immunosorbent assay; TLRs, toll-like receptors; SEAP, secreted embryonic alkaline phosphatase.

Techniques Used: Enzyme-linked Immunosorbent Assay

NIPAM-hemin induced the production of IFN-γ and IL-6, and had a lesser effect on the induction of IL-1β, in PBMCs. PBMCs were stimulated with NIPAM-hemin, hemin, NIPAM, or poly-NIPAM (MW=66,400). The levels of ( A ) IFN-γ, ( B ) IL-6, and ( C ) IL-1β were determined after 48 h. The concentration of each stimulant was 500 μg/mL. Hemin was dissolved in DMSO, while the other compounds were dissolved in sterilized deionized water. Notes: Data are expressed as the mean±SD (n=3). * P
Figure Legend Snippet: NIPAM-hemin induced the production of IFN-γ and IL-6, and had a lesser effect on the induction of IL-1β, in PBMCs. PBMCs were stimulated with NIPAM-hemin, hemin, NIPAM, or poly-NIPAM (MW=66,400). The levels of ( A ) IFN-γ, ( B ) IL-6, and ( C ) IL-1β were determined after 48 h. The concentration of each stimulant was 500 μg/mL. Hemin was dissolved in DMSO, while the other compounds were dissolved in sterilized deionized water. Notes: Data are expressed as the mean±SD (n=3). * P

Techniques Used: Concentration Assay

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    Cellular Technology Ltd integration free human pbmc derived ipscs
    Schematic neural induction diagrams and characterization of the NSPCs generated from human <t>PBMC-derived</t> <t>iPSCs.</t> a Schematics of the NSPC induction protocols used in this study. (Scale = 200 μm for the images of neurospheres.) ( b , c , d ) Representative data taken by 1210B2-NSPCs for characterization analysis of the NSPCs. b Cell surface markers of the induced NSPCs. c The quantitative RT-PCR analysis results are depicted by ΔCt values. Quantitative RT − PCR analysis confirmed the decrease in the iPSC markers, and an increase in NSPC markers following the differentiation of iPSCs into NSPCs. ( n = 2 for each iPSC-NSPC lines) d Representative immunocytochemistry data collected to confirm the differentiation potential of NSPCs into neuronal and glial lineages. (Scale = 100 μm.) e The correlation coefficients of the expression profiles among the NSPCs. The microarray profiles were similar between all the NSPCs regardless of the induction protocols or iPSCs. ( n = 2 for each iPSC-NSPC lines) See also Additional file 1 : Figure S1 and Additional file 2 : Table S1
    Integration Free Human Pbmc Derived Ipscs, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellular Technology Ltd adcc assay cryopreserved human pbmcs
    FcγRIIIa activation and antibody-dependent cell-mediated cytotoxicity <t>(ADCC).</t> ( A ) CD20 binding-dependent activation of FcγRIIIa was measured by using Daudi (target) and Jurkat/FcγRIIIa/NFAT-Luc (effector) cells. Fold increases in luciferase activity were plotted against the mAbs concentration (n = 3, bars indicate SEM). ( B ) The levels of ADCC against Daudi cells were measured by using human <t>PBMCs</t> as effector cells. The percentages of cell lysis were plotted against the mAbs concentration (n = 3, bars indicate SEM).
    Adcc Assay Cryopreserved Human Pbmcs, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellular Technology Ltd analysis healthy human pbmcs
    Analysis of immune cell subset composition in Pseudomonas aeruginosa –positive and –negative samples. ( A and B ) Raw ( A ) and T cell–normalized ( B ) composition scores of all cell subsets in HC and CF subgroups. ( C ) Cell numbers (left) and cell subset frequencies (right) of <t>CD14</t> + CD16 − monocytes in <t>PBMCs</t> stimulated by CF and HC serum. ( D ) Composition scores of 10 major cell subsets in 6 CF probands (CFPs) compared with those of their mothers. Lines connecting the dots indicate pairs of children and mothers. Cell subset scores were evaluated across all 143 subjects in the validation cohort.
    Analysis Healthy Human Pbmcs, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 84/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Schematic neural induction diagrams and characterization of the NSPCs generated from human PBMC-derived iPSCs. a Schematics of the NSPC induction protocols used in this study. (Scale = 200 μm for the images of neurospheres.) ( b , c , d ) Representative data taken by 1210B2-NSPCs for characterization analysis of the NSPCs. b Cell surface markers of the induced NSPCs. c The quantitative RT-PCR analysis results are depicted by ΔCt values. Quantitative RT − PCR analysis confirmed the decrease in the iPSC markers, and an increase in NSPC markers following the differentiation of iPSCs into NSPCs. ( n = 2 for each iPSC-NSPC lines) d Representative immunocytochemistry data collected to confirm the differentiation potential of NSPCs into neuronal and glial lineages. (Scale = 100 μm.) e The correlation coefficients of the expression profiles among the NSPCs. The microarray profiles were similar between all the NSPCs regardless of the induction protocols or iPSCs. ( n = 2 for each iPSC-NSPC lines) See also Additional file 1 : Figure S1 and Additional file 2 : Table S1

    Journal: Molecular Brain

    Article Title: Pathological classification of human iPSC-derived neural stem/progenitor cells towards safety assessment of transplantation therapy for CNS diseases

    doi: 10.1186/s13041-016-0265-8

    Figure Lengend Snippet: Schematic neural induction diagrams and characterization of the NSPCs generated from human PBMC-derived iPSCs. a Schematics of the NSPC induction protocols used in this study. (Scale = 200 μm for the images of neurospheres.) ( b , c , d ) Representative data taken by 1210B2-NSPCs for characterization analysis of the NSPCs. b Cell surface markers of the induced NSPCs. c The quantitative RT-PCR analysis results are depicted by ΔCt values. Quantitative RT − PCR analysis confirmed the decrease in the iPSC markers, and an increase in NSPC markers following the differentiation of iPSCs into NSPCs. ( n = 2 for each iPSC-NSPC lines) d Representative immunocytochemistry data collected to confirm the differentiation potential of NSPCs into neuronal and glial lineages. (Scale = 100 μm.) e The correlation coefficients of the expression profiles among the NSPCs. The microarray profiles were similar between all the NSPCs regardless of the induction protocols or iPSCs. ( n = 2 for each iPSC-NSPC lines) See also Additional file 1 : Figure S1 and Additional file 2 : Table S1

    Article Snippet: Cell culture Three lines of integration free human PBMC-derived iPSCs (1210B2, 1231A3, and 1201C1), which were established from ePBMCs® from the Cellular Technology Limited (OH, USA) at Center for iPS Cell Research and Application (CiRA: Kyoto, Japan) by an integration-free method [ ], were used.

    Techniques: Generated, Derivative Assay, Quantitative RT-PCR, Immunocytochemistry, Expressing, Microarray

    FcγRIIIa activation and antibody-dependent cell-mediated cytotoxicity (ADCC). ( A ) CD20 binding-dependent activation of FcγRIIIa was measured by using Daudi (target) and Jurkat/FcγRIIIa/NFAT-Luc (effector) cells. Fold increases in luciferase activity were plotted against the mAbs concentration (n = 3, bars indicate SEM). ( B ) The levels of ADCC against Daudi cells were measured by using human PBMCs as effector cells. The percentages of cell lysis were plotted against the mAbs concentration (n = 3, bars indicate SEM).

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    Article Title: Characterization of anti-CD20 monoclonal antibody produced by transgenic silkworms (Bombyx mori)

    doi: 10.1080/19420862.2015.1078054

    Figure Lengend Snippet: FcγRIIIa activation and antibody-dependent cell-mediated cytotoxicity (ADCC). ( A ) CD20 binding-dependent activation of FcγRIIIa was measured by using Daudi (target) and Jurkat/FcγRIIIa/NFAT-Luc (effector) cells. Fold increases in luciferase activity were plotted against the mAbs concentration (n = 3, bars indicate SEM). ( B ) The levels of ADCC against Daudi cells were measured by using human PBMCs as effector cells. The percentages of cell lysis were plotted against the mAbs concentration (n = 3, bars indicate SEM).

    Article Snippet: ADCC assay Cryopreserved human PBMCs (Cellular Technology Limited) were thawed just before the assay according to the manufacturer's protocol.

    Techniques: Activation Assay, Binding Assay, Luciferase, Activity Assay, Concentration Assay, Lysis

    Analysis of immune cell subset composition in Pseudomonas aeruginosa –positive and –negative samples. ( A and B ) Raw ( A ) and T cell–normalized ( B ) composition scores of all cell subsets in HC and CF subgroups. ( C ) Cell numbers (left) and cell subset frequencies (right) of CD14 + CD16 − monocytes in PBMCs stimulated by CF and HC serum. ( D ) Composition scores of 10 major cell subsets in 6 CF probands (CFPs) compared with those of their mothers. Lines connecting the dots indicate pairs of children and mothers. Cell subset scores were evaluated across all 143 subjects in the validation cohort.

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: Cystic Fibrosis Plasma Blunts the Immune Response to Bacterial Infection

    doi: 10.1165/rcmb.2018-0114OC

    Figure Lengend Snippet: Analysis of immune cell subset composition in Pseudomonas aeruginosa –positive and –negative samples. ( A and B ) Raw ( A ) and T cell–normalized ( B ) composition scores of all cell subsets in HC and CF subgroups. ( C ) Cell numbers (left) and cell subset frequencies (right) of CD14 + CD16 − monocytes in PBMCs stimulated by CF and HC serum. ( D ) Composition scores of 10 major cell subsets in 6 CF probands (CFPs) compared with those of their mothers. Lines connecting the dots indicate pairs of children and mothers. Cell subset scores were evaluated across all 143 subjects in the validation cohort.

    Article Snippet: CD14+ CD16− Monocytes Isolation and Analysis Healthy human PBMCs (UPN727; Cellular Technology Limited) were seeded (7.5 × 105 per well) and cultured with serum for 9 hours.

    Techniques:

    The evaluation of the immunostimulatory effects of NIPAM-hemin. Abbreviations: NIPAM, N -isopropylacrylamide; hemin, ferriprotoporphyrin IX chloride; IFN-γ, interferon-γ; IL, interleukin; NK, natural killer; PBMCs, peripheral blood mononuclear cells; ELISA, enzyme-linked immunosorbent assay; TLRs, toll-like receptors; SEAP, secreted embryonic alkaline phosphatase.

    Journal: International Journal of Nanomedicine

    Article Title: Synthesis of a hemin-containing copolymer as a novel immunostimulator that induces IFN-gamma production

    doi: 10.2147/IJN.S166259

    Figure Lengend Snippet: The evaluation of the immunostimulatory effects of NIPAM-hemin. Abbreviations: NIPAM, N -isopropylacrylamide; hemin, ferriprotoporphyrin IX chloride; IFN-γ, interferon-γ; IL, interleukin; NK, natural killer; PBMCs, peripheral blood mononuclear cells; ELISA, enzyme-linked immunosorbent assay; TLRs, toll-like receptors; SEAP, secreted embryonic alkaline phosphatase.

    Article Snippet: Stimulation of human PBMCs with NIPAM-hemin Commercially available frozen PBMCs were purchased from Cellular Technology Limited (Shaker Heights, OH, USA).

    Techniques: Enzyme-linked Immunosorbent Assay

    NIPAM-hemin induced the production of IFN-γ and IL-6, and had a lesser effect on the induction of IL-1β, in PBMCs. PBMCs were stimulated with NIPAM-hemin, hemin, NIPAM, or poly-NIPAM (MW=66,400). The levels of ( A ) IFN-γ, ( B ) IL-6, and ( C ) IL-1β were determined after 48 h. The concentration of each stimulant was 500 μg/mL. Hemin was dissolved in DMSO, while the other compounds were dissolved in sterilized deionized water. Notes: Data are expressed as the mean±SD (n=3). * P

    Journal: International Journal of Nanomedicine

    Article Title: Synthesis of a hemin-containing copolymer as a novel immunostimulator that induces IFN-gamma production

    doi: 10.2147/IJN.S166259

    Figure Lengend Snippet: NIPAM-hemin induced the production of IFN-γ and IL-6, and had a lesser effect on the induction of IL-1β, in PBMCs. PBMCs were stimulated with NIPAM-hemin, hemin, NIPAM, or poly-NIPAM (MW=66,400). The levels of ( A ) IFN-γ, ( B ) IL-6, and ( C ) IL-1β were determined after 48 h. The concentration of each stimulant was 500 μg/mL. Hemin was dissolved in DMSO, while the other compounds were dissolved in sterilized deionized water. Notes: Data are expressed as the mean±SD (n=3). * P

    Article Snippet: Stimulation of human PBMCs with NIPAM-hemin Commercially available frozen PBMCs were purchased from Cellular Technology Limited (Shaker Heights, OH, USA).

    Techniques: Concentration Assay