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Astarte Biologics human pbmcs
Human Pbmcs, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human pbmcs/product/Astarte Biologics
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
human pbmcs - by Bioz Stars, 2020-09
91/100 stars

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Isolation:

Article Title: A DNA Vaccine Protects Human Immune Cells against Zika Virus Infection in Humanized Mice
Article Snippet: .. 2.10 In Vitro Infection of Human B Cells Human B cells were isolated from human PBMCs purchased from Astarte Biologics (Bothell, WA) using CD19 positive selection kit (Stemcell Technologies, Canada). .. The purities of the isolated CD19 + cells are 95–100%.

Selection:

Article Title: A DNA Vaccine Protects Human Immune Cells against Zika Virus Infection in Humanized Mice
Article Snippet: .. 2.10 In Vitro Infection of Human B Cells Human B cells were isolated from human PBMCs purchased from Astarte Biologics (Bothell, WA) using CD19 positive selection kit (Stemcell Technologies, Canada). .. The purities of the isolated CD19 + cells are 95–100%.

In Vitro:

Article Title: A DNA Vaccine Protects Human Immune Cells against Zika Virus Infection in Humanized Mice
Article Snippet: .. 2.10 In Vitro Infection of Human B Cells Human B cells were isolated from human PBMCs purchased from Astarte Biologics (Bothell, WA) using CD19 positive selection kit (Stemcell Technologies, Canada). .. The purities of the isolated CD19 + cells are 95–100%.

Infection:

Article Title: A DNA Vaccine Protects Human Immune Cells against Zika Virus Infection in Humanized Mice
Article Snippet: .. 2.10 In Vitro Infection of Human B Cells Human B cells were isolated from human PBMCs purchased from Astarte Biologics (Bothell, WA) using CD19 positive selection kit (Stemcell Technologies, Canada). .. The purities of the isolated CD19 + cells are 95–100%.

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    Astarte Biologics hiv infection human pbmcs
    IFN-α is induced after <t>HIV-1</t> infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in <t>PBMCs</t> isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.
    Hiv Infection Human Pbmcs, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv infection human pbmcs/product/Astarte Biologics
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiv infection human pbmcs - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    90
    Astarte Biologics human pbmc reconstitution
    IFN-α is induced after <t>HIV-1</t> infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in <t>PBMCs</t> isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.
    Human Pbmc Reconstitution, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pbmc reconstitution/product/Astarte Biologics
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human pbmc reconstitution - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    92
    Astarte Biologics human peripheral blood mononuclear cells pbmcs
    IFN-α is induced after <t>HIV-1</t> infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in <t>PBMCs</t> isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.
    Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human peripheral blood mononuclear cells pbmcs/product/Astarte Biologics
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    human peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    IFN-α is induced after HIV-1 infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in PBMCs isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.

    Journal: Oncotarget

    Article Title: Gene therapy with plasmids encoding IFN-β or IFN-α14 confers long-term resistance to HIV-1 in humanized mice

    doi: 10.18632/oncotarget.12512

    Figure Lengend Snippet: IFN-α is induced after HIV-1 infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in PBMCs isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.

    Article Snippet: Human PBMC reconstitution and HIV infection Human PBMCs were obtained from Astarte Biologics (Bothell, WA), and these cells (1×107 ) were injected through the tail vein into 6-8-week-old NSG mice.

    Techniques: Infection, Mouse Assay, Expressing, Enzyme-linked Immunosorbent Assay, Negative Control, Plasmid Preparation, Injection, Positive Control, Isolation, Quantitative RT-PCR, MANN-WHITNEY

    Injection of plasmids expressing subtypes of IFN-I results in prolonged upregulation of ISGs A. Hu-PBL mice were hydrodynamically injected with plasmids encoding IFN-α2, −α6, −α8, −α14 (left panel), or -IFN-beta (right panel) subtypes, and their sera were tested for IFN-I by ELISA at the indicated times after injection. n = 5 mice/group. B. PBMCs obtained on the indicated days after plasmid injection were tested for upregulation of the indicated ISGs over time by qRT-PCR. C. PBMCs obtained on days 2 and 40 after plasmid injection were tested for expression of indicated HIV-1 restriction factors by qPCR. Kruskal Wallis test followed by Dunn's multiple comparison analysis was used for statistical evaluation. * p

    Journal: Oncotarget

    Article Title: Gene therapy with plasmids encoding IFN-β or IFN-α14 confers long-term resistance to HIV-1 in humanized mice

    doi: 10.18632/oncotarget.12512

    Figure Lengend Snippet: Injection of plasmids expressing subtypes of IFN-I results in prolonged upregulation of ISGs A. Hu-PBL mice were hydrodynamically injected with plasmids encoding IFN-α2, −α6, −α8, −α14 (left panel), or -IFN-beta (right panel) subtypes, and their sera were tested for IFN-I by ELISA at the indicated times after injection. n = 5 mice/group. B. PBMCs obtained on the indicated days after plasmid injection were tested for upregulation of the indicated ISGs over time by qRT-PCR. C. PBMCs obtained on days 2 and 40 after plasmid injection were tested for expression of indicated HIV-1 restriction factors by qPCR. Kruskal Wallis test followed by Dunn's multiple comparison analysis was used for statistical evaluation. * p

    Article Snippet: Human PBMC reconstitution and HIV infection Human PBMCs were obtained from Astarte Biologics (Bothell, WA), and these cells (1×107 ) were injected through the tail vein into 6-8-week-old NSG mice.

    Techniques: Injection, Expressing, Mouse Assay, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    IFN-α is induced after HIV-1 infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in PBMCs isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.

    Journal: Oncotarget

    Article Title: Gene therapy with plasmids encoding IFN-β or IFN-α14 confers long-term resistance to HIV-1 in humanized mice

    doi: 10.18632/oncotarget.12512

    Figure Lengend Snippet: IFN-α is induced after HIV-1 infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in PBMCs isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.

    Article Snippet: Human PBMC reconstitution and HIV infection Human PBMCs were obtained from Astarte Biologics (Bothell, WA), and these cells (1×107 ) were injected through the tail vein into 6-8-week-old NSG mice.

    Techniques: Infection, Mouse Assay, Expressing, Enzyme-linked Immunosorbent Assay, Negative Control, Plasmid Preparation, Injection, Positive Control, Isolation, Quantitative RT-PCR, MANN-WHITNEY

    Injection of plasmids expressing subtypes of IFN-I results in prolonged upregulation of ISGs A. Hu-PBL mice were hydrodynamically injected with plasmids encoding IFN-α2, −α6, −α8, −α14 (left panel), or -IFN-beta (right panel) subtypes, and their sera were tested for IFN-I by ELISA at the indicated times after injection. n = 5 mice/group. B. PBMCs obtained on the indicated days after plasmid injection were tested for upregulation of the indicated ISGs over time by qRT-PCR. C. PBMCs obtained on days 2 and 40 after plasmid injection were tested for expression of indicated HIV-1 restriction factors by qPCR. Kruskal Wallis test followed by Dunn's multiple comparison analysis was used for statistical evaluation. * p

    Journal: Oncotarget

    Article Title: Gene therapy with plasmids encoding IFN-β or IFN-α14 confers long-term resistance to HIV-1 in humanized mice

    doi: 10.18632/oncotarget.12512

    Figure Lengend Snippet: Injection of plasmids expressing subtypes of IFN-I results in prolonged upregulation of ISGs A. Hu-PBL mice were hydrodynamically injected with plasmids encoding IFN-α2, −α6, −α8, −α14 (left panel), or -IFN-beta (right panel) subtypes, and their sera were tested for IFN-I by ELISA at the indicated times after injection. n = 5 mice/group. B. PBMCs obtained on the indicated days after plasmid injection were tested for upregulation of the indicated ISGs over time by qRT-PCR. C. PBMCs obtained on days 2 and 40 after plasmid injection were tested for expression of indicated HIV-1 restriction factors by qPCR. Kruskal Wallis test followed by Dunn's multiple comparison analysis was used for statistical evaluation. * p

    Article Snippet: Human PBMC reconstitution and HIV infection Human PBMCs were obtained from Astarte Biologics (Bothell, WA), and these cells (1×107 ) were injected through the tail vein into 6-8-week-old NSG mice.

    Techniques: Injection, Expressing, Mouse Assay, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction