human pbmcs  (ATCC)


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  • 99
    Name:
    Primary Peripheral Blood Mononuclear Cells PBMC Normal Human
    Description:

    Catalog Number:
    pcs-800-011
    Price:
    None
    Applications:
    Applications for use include the study of immunology, infection, cancer, hematology, and t-cell suppression assay.
    Host:
    Homo sapiens, human
    Cell Type:
    Mononuclear
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    Structured Review

    ATCC human pbmcs
    CpG-B DNA, not CpG-A DNA, induces phosphorylation of <t>PKD</t> proteins in murine splenic B cells, cDCs, and pDCs, and human <t>PBMCs</t>

    https://www.bioz.com/result/human pbmcs/product/ATCC
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human pbmcs - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling"

    Article Title: Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling

    Journal:

    doi:

    CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs
    Figure Legend Snippet: CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs

    Techniques Used:

    2) Product Images from "Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling"

    Article Title: Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling

    Journal:

    doi:

    CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs
    Figure Legend Snippet: CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs

    Techniques Used:

    3) Product Images from "Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling"

    Article Title: Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling

    Journal:

    doi:

    CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs
    Figure Legend Snippet: CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs

    Techniques Used:

    4) Product Images from "Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling"

    Article Title: Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling

    Journal:

    doi:

    CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs
    Figure Legend Snippet: CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs

    Techniques Used:

    5) Product Images from "Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling"

    Article Title: Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling

    Journal:

    doi:

    CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs
    Figure Legend Snippet: CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs

    Techniques Used:

    6) Product Images from "Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling"

    Article Title: Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling

    Journal:

    doi:

    CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs
    Figure Legend Snippet: CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs

    Techniques Used:

    7) Product Images from "Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling"

    Article Title: Protein Kinase D1: A New Component in Toll-Like Receptor 9 Signaling

    Journal:

    doi:

    CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs
    Figure Legend Snippet: CpG-B DNA, not CpG-A DNA, induces phosphorylation of PKD proteins in murine splenic B cells, cDCs, and pDCs, and human PBMCs

    Techniques Used:

    Related Articles

    Trypan Blue Exclusion Assay:

    Article Title: Antibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles
    Article Snippet: .. Cytotoxicity test The effects of Ag-MWCNTs on the viability of mouse liver hepatocytes (AML 12; Chungnam University, Daejeon Korea) and human peripheral blood mononuclear cells (PBMCs) (American Type Culture Collection [ATCC], Manassas, VA, USA) were evaluated using a trypan blue exclusion assay and a LIVE/DEAD® Viability/Cytotoxicity Kit. .. Cultured AML 12 cells and human PBMCs were plated in six-well plates (1×105 cells per well) in Dulbecco’s Modified Eagle’s Medium (DMEM) and Roswell Park Memorial Institute medium (RPMI), respectively, each supplemented with 10% (v/v) fetal bovine serum and 1% sterile antibiotic.

    Isolation:

    Article Title: A New Family of Small-Molecule CD4-Mimetic Compounds Contacts Highly Conserved Aspartic Acid 368 of HIV-1 gp120 and Mediates Antibody-Dependent Cellular Cytotoxicity
    Article Snippet: .. Primary human peripheral blood mononuclear cells (PBMCs) and CD4+ T cells were isolated, activated, and cultured as previously described ( , ). ..

    Quantitation Assay:

    Article Title: Modeling Cell-Specific Dynamics and Regulation of the Common Gamma Chain Cytokines
    Article Snippet: .. Receptor abundance quantitation Cryopreserved PBMCs (ATCC, PCS-800-011, lot#81115172) were thawed to room temperature and slowly diluted with 9 mL pre-warmed RPMI-1640 medium (Gibco, 11875-093) supplemented with 10% fetal bovine serum (FBS, Seradigm, 1500-500, lot#322B15). ..

    Labeling:

    Article Title: Nkx2‐5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E–Deficient Mice
    Article Snippet: .. Fresh adult human PBMCs (PCS‐800‐011; ATCC) were labeled with calcein acetomethoxy (AM) dye: PBMCs were pelleted at 240g for 10 minutes, resuspended in 1 mL of culture medium with 2.5 μmol/L of calcein AM from the kit, and incubated at 37°C (5% CO2 ) for 30 minutes. .. PBMCs were then washed 3 times with HAEC media and added to HAEC cells (150 000 labeled PBMCs per chamber).

    Incubation:

    Article Title: Nkx2‐5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E–Deficient Mice
    Article Snippet: .. Fresh adult human PBMCs (PCS‐800‐011; ATCC) were labeled with calcein acetomethoxy (AM) dye: PBMCs were pelleted at 240g for 10 minutes, resuspended in 1 mL of culture medium with 2.5 μmol/L of calcein AM from the kit, and incubated at 37°C (5% CO2 ) for 30 minutes. .. PBMCs were then washed 3 times with HAEC media and added to HAEC cells (150 000 labeled PBMCs per chamber).

    other:

    Article Title: Potency of Combining Eucalyptus camaldulensis subsp. camaldulensis with Low-Dose Cisplatin in A549 Human Lung Adenocarcinomas and MCF-7 Breast Adenocarcinoma
    Article Snippet: Peripheral Blood Mononuclear Cells (PBMC)Human peripheral blood mononuclear cells (PBMCs) obtained from ATCC, Manassas, VA, USA, comprising lymphocytes (B-cells, T-cells, and NK-cells), monocytes, and dendritic cells, were frequently used for the evaluation of immune responses.

    Cell Culture:

    Article Title: DNA-PKcs controls calcineurin mediated IL-2 production in T lymphocytes
    Article Snippet: .. Cell culture Human peripheral blood mononuclear cells (PBMC,) and Jurkat cells were purchased from ATCC (PCS-800-011, Manassas, VA). ..

    Article Title: Tendency of K562 Chronic Myeloid Leukemia Cells Towards Cell Reprogramming
    Article Snippet: .. Cell Culture Human CML cell line K562 and human peripheral blood mononuclear cells (PBMCs) were obtained from ATCC and Lonza, respectively. .. Cells were cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 50 U/mL penicillin, 50 µg/mL streptomycin, and 1% L‐glutamine at 37 °C in 5% CO2 .

    Article Title: A New Family of Small-Molecule CD4-Mimetic Compounds Contacts Highly Conserved Aspartic Acid 368 of HIV-1 gp120 and Mediates Antibody-Dependent Cellular Cytotoxicity
    Article Snippet: .. Primary human peripheral blood mononuclear cells (PBMCs) and CD4+ T cells were isolated, activated, and cultured as previously described ( , ). ..

    Gradient Centrifugation:

    Article Title: Critical Role for the NLRP3 Inflammasome in Mediating IL-1β Production in Shigella sonnei-Infected Macrophages
    Article Snippet: .. THP-1 macrophages were differentiated from THP-1 monocytes by treatment with 50 nM PMA for 48 h. Human peripheral blood mononuclear cells (PBMCs) were separated from whole blood from healthy volunteers by density gradient centrifugation using Histopaque-1077 , and all experimental protocols were performed in accordance with the guidelines and regulations provided and accepted by the Institutional Review Board of the Tri-Service General Hospital, National Defense Medical Center and the volunteers' informed consent (TSGH-IRB-2-106-05-190 and TSGH-IRB-2-106-05-009). .. Mouse primary bone marrow derived macrophages (BMDM) were prepared from bone marrow collected from C57BL/6 mouse femur and tibia by differentiating in the M-CSF containing medium for 7 days.

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  • 99
    ATCC peripheral blood mononuclear cells pbmc human peripheral blood mononuclear cells pbmcs
    Cell viability of peripheral blood mononuclear cells <t>(PBMC)</t> after treatment for 24 h. ( A ) Cell viability of <t>PBMC</t> cells after treatment with the ethanolic extract of E. camaldulensis for 24 h. ( B ) Cell viability of PBMC cells after treatment with increasing concentrations of CDDP for 24 h. ( C ) Cell viability of PBMC cells after treatment with the aqueous extract of E. camaldulensis for 24 h. ( D ) Cell viability of PBMC 24 h after treatment with CDDP (4 µg/mL) combined with the aqueous extract (AE 75 µg/mL). ( E ) Cell viability of PBMC 24 h after treatment with CDDP (4 µg/mL) combined with the aqueous extract (AE 150 µg/mL) (* p
    Peripheral Blood Mononuclear Cells Pbmc Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peripheral blood mononuclear cells pbmc human peripheral blood mononuclear cells pbmcs/product/ATCC
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    peripheral blood mononuclear cells pbmc human peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
    99/100 stars
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    Image Search Results


    Cell viability of peripheral blood mononuclear cells (PBMC) after treatment for 24 h. ( A ) Cell viability of PBMC cells after treatment with the ethanolic extract of E. camaldulensis for 24 h. ( B ) Cell viability of PBMC cells after treatment with increasing concentrations of CDDP for 24 h. ( C ) Cell viability of PBMC cells after treatment with the aqueous extract of E. camaldulensis for 24 h. ( D ) Cell viability of PBMC 24 h after treatment with CDDP (4 µg/mL) combined with the aqueous extract (AE 75 µg/mL). ( E ) Cell viability of PBMC 24 h after treatment with CDDP (4 µg/mL) combined with the aqueous extract (AE 150 µg/mL) (* p

    Journal: Medicines

    Article Title: Potency of Combining Eucalyptus camaldulensis subsp. camaldulensis with Low-Dose Cisplatin in A549 Human Lung Adenocarcinomas and MCF-7 Breast Adenocarcinoma

    doi: 10.3390/medicines7080040

    Figure Lengend Snippet: Cell viability of peripheral blood mononuclear cells (PBMC) after treatment for 24 h. ( A ) Cell viability of PBMC cells after treatment with the ethanolic extract of E. camaldulensis for 24 h. ( B ) Cell viability of PBMC cells after treatment with increasing concentrations of CDDP for 24 h. ( C ) Cell viability of PBMC cells after treatment with the aqueous extract of E. camaldulensis for 24 h. ( D ) Cell viability of PBMC 24 h after treatment with CDDP (4 µg/mL) combined with the aqueous extract (AE 75 µg/mL). ( E ) Cell viability of PBMC 24 h after treatment with CDDP (4 µg/mL) combined with the aqueous extract (AE 150 µg/mL) (* p

    Article Snippet: Peripheral Blood Mononuclear Cells (PBMC)Human peripheral blood mononuclear cells (PBMCs) obtained from ATCC, Manassas, VA, USA, comprising lymphocytes (B-cells, T-cells, and NK-cells), monocytes, and dendritic cells, were frequently used for the evaluation of immune responses.

    Techniques:

    S. sonnei induces the secretion of IL-1β, IL-18, NLRP3, ASC, and active caspase-1 in macrophages. (A) J774A.1 macrophages, THP-1 macrophages, PBMCs or BMDM were primed with 1 μg/ml LPS for 4 h and then infected with S. sonnei for an additional 20 h. The levels of IL-1β in the supernatants were measured by ELISA. (B–E) J774A.1 macrophages or THP-1 macrophages were primed with 1 μg/ml LPS for 4 h followed and then infected with S. sonnei for an additional 20 h or stimulated with 5 mM ATP for an additional 0.5 h. The levels of IL-1β (B) , IL-18 (C) , caspase-1 (D) , NLRP3, and ASC (E) in the supernatants were measured by Western blotting. The ELISA data are expressed as the mean ± SD of four separate experiments. The Western blotting results are representative of three different experiments. * and *** indicate significant differences at the levels of p

    Journal: Frontiers in Immunology

    Article Title: Critical Role for the NLRP3 Inflammasome in Mediating IL-1β Production in Shigella sonnei-Infected Macrophages

    doi: 10.3389/fimmu.2020.01115

    Figure Lengend Snippet: S. sonnei induces the secretion of IL-1β, IL-18, NLRP3, ASC, and active caspase-1 in macrophages. (A) J774A.1 macrophages, THP-1 macrophages, PBMCs or BMDM were primed with 1 μg/ml LPS for 4 h and then infected with S. sonnei for an additional 20 h. The levels of IL-1β in the supernatants were measured by ELISA. (B–E) J774A.1 macrophages or THP-1 macrophages were primed with 1 μg/ml LPS for 4 h followed and then infected with S. sonnei for an additional 20 h or stimulated with 5 mM ATP for an additional 0.5 h. The levels of IL-1β (B) , IL-18 (C) , caspase-1 (D) , NLRP3, and ASC (E) in the supernatants were measured by Western blotting. The ELISA data are expressed as the mean ± SD of four separate experiments. The Western blotting results are representative of three different experiments. * and *** indicate significant differences at the levels of p

    Article Snippet: THP-1 macrophages were differentiated from THP-1 monocytes by treatment with 50 nM PMA for 48 h. Human peripheral blood mononuclear cells (PBMCs) were separated from whole blood from healthy volunteers by density gradient centrifugation using Histopaque-1077 , and all experimental protocols were performed in accordance with the guidelines and regulations provided and accepted by the Institutional Review Board of the Tri-Service General Hospital, National Defense Medical Center and the volunteers' informed consent (TSGH-IRB-2-106-05-190 and TSGH-IRB-2-106-05-009).

    Techniques: Infection, Enzyme-linked Immunosorbent Assay, Western Blot

    Inhibition of DNA-PKcs in T cells and PBMCs blocks IL-2 production. A) Jurkat cells were treated with the DNA-PKcs inhibitor NU7441 at varying concentrations for 48 hours and no significant reduction in viability was detected. B) Jurkat cells were stimulated with PMA (50 ng/mL)+PHA (1 μg/mL), treated with NU7441, and analyzed for IL-2 production 24 hours later. NU7441 treatment significantly blocked IL-2 secretion. C) IL-2 production stimulated by activation of Jurkat cells with anti-CD28/CD3 dynabeads at a 1:1 ratio for 24 hours was inhibited by NU7441 treatment. D) Treatment of Jurkat cells with shRNA reduced DNA-PKcs expression at 2.5 and 5 μg as seen by western blot analysis. Loss of DNA-PKcs expression significantly reduced IL-2 production. E) NU7441 significantly reduce IL-2 production following activation with PHA+PMA in PBMCs. ** p

    Journal: PLoS ONE

    Article Title: DNA-PKcs controls calcineurin mediated IL-2 production in T lymphocytes

    doi: 10.1371/journal.pone.0181608

    Figure Lengend Snippet: Inhibition of DNA-PKcs in T cells and PBMCs blocks IL-2 production. A) Jurkat cells were treated with the DNA-PKcs inhibitor NU7441 at varying concentrations for 48 hours and no significant reduction in viability was detected. B) Jurkat cells were stimulated with PMA (50 ng/mL)+PHA (1 μg/mL), treated with NU7441, and analyzed for IL-2 production 24 hours later. NU7441 treatment significantly blocked IL-2 secretion. C) IL-2 production stimulated by activation of Jurkat cells with anti-CD28/CD3 dynabeads at a 1:1 ratio for 24 hours was inhibited by NU7441 treatment. D) Treatment of Jurkat cells with shRNA reduced DNA-PKcs expression at 2.5 and 5 μg as seen by western blot analysis. Loss of DNA-PKcs expression significantly reduced IL-2 production. E) NU7441 significantly reduce IL-2 production following activation with PHA+PMA in PBMCs. ** p

    Article Snippet: Cell culture Human peripheral blood mononuclear cells (PBMC,) and Jurkat cells were purchased from ATCC (PCS-800-011, Manassas, VA).

    Techniques: Inhibition, Activation Assay, shRNA, Expressing, Western Blot

    NK 2 homeobox 5 (Nkx2‐5) inhibits monocyte‐endothelial adhesion and decreases expression of adhesion molecules in early atherosclerosis. A, Upper panel: representative images used for quantification of peripheral blood monocytes ( PBMC ; green, calcein AM ) attached to aortic endothelial cells ( HAEC s, blue, DAPI ). Middle panel: effects of Nkx2‐5 on expression of vascular cell adhesion molecule‐1 ( VCAM ‐1) in endothelial cells (representative immunofluorescence image: green for VCAM ‐1 and blue for cell nuclei stained with DAPI ). Lower panel: effects of Nkx2‐5 on expression of E‐selectin in endothelial cells (representative immunofluorescence image: red for E‐selectin and blue for cell nuclei stained with DAPI ). B, Quantification of adhesion. The number of PBMC adhered to per 100 HAEC s was calculated. Results are expressed as a percent of values determined in the Ad‐ EV ‐treated group. Data represent the mean± SEM of 3 independent experiments. C, Representative immunoblot for intercellular adhesion molecule‐1 ( ICAM ‐1), VCAM ‐1, E‐selectin, and P‐selectin in endothelial cells infected with Ad‐ EV or Ad‐Nkx2‐5. D, Cross‐sections of early atherosclerotic lesions in aortic sinus were immunostained with antibodies against von Willebrand factor ( vWF ), VCAM ‐1, and E‐selectin. Staining with rabbit IgG isotype was used as the negative control. E, Quantification of histochemical staining of VCAM ‐1 and E‐selectin. Positive stained areas were quantified as a percentage of total plaque area or plaque endothelium. Data are expressed as mean± SEM (n=12 per group). AM indicates acetomethoxy; DAPI, 4′,6‐diamidino‐2‐phenylindole; HAECs, human aortic endothelial cells; IgG, immunoglobulin G; TNFα, tumor necrosis factor alpha.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Nkx2‐5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E–Deficient Mice

    doi: 10.1161/JAHA.116.004440

    Figure Lengend Snippet: NK 2 homeobox 5 (Nkx2‐5) inhibits monocyte‐endothelial adhesion and decreases expression of adhesion molecules in early atherosclerosis. A, Upper panel: representative images used for quantification of peripheral blood monocytes ( PBMC ; green, calcein AM ) attached to aortic endothelial cells ( HAEC s, blue, DAPI ). Middle panel: effects of Nkx2‐5 on expression of vascular cell adhesion molecule‐1 ( VCAM ‐1) in endothelial cells (representative immunofluorescence image: green for VCAM ‐1 and blue for cell nuclei stained with DAPI ). Lower panel: effects of Nkx2‐5 on expression of E‐selectin in endothelial cells (representative immunofluorescence image: red for E‐selectin and blue for cell nuclei stained with DAPI ). B, Quantification of adhesion. The number of PBMC adhered to per 100 HAEC s was calculated. Results are expressed as a percent of values determined in the Ad‐ EV ‐treated group. Data represent the mean± SEM of 3 independent experiments. C, Representative immunoblot for intercellular adhesion molecule‐1 ( ICAM ‐1), VCAM ‐1, E‐selectin, and P‐selectin in endothelial cells infected with Ad‐ EV or Ad‐Nkx2‐5. D, Cross‐sections of early atherosclerotic lesions in aortic sinus were immunostained with antibodies against von Willebrand factor ( vWF ), VCAM ‐1, and E‐selectin. Staining with rabbit IgG isotype was used as the negative control. E, Quantification of histochemical staining of VCAM ‐1 and E‐selectin. Positive stained areas were quantified as a percentage of total plaque area or plaque endothelium. Data are expressed as mean± SEM (n=12 per group). AM indicates acetomethoxy; DAPI, 4′,6‐diamidino‐2‐phenylindole; HAECs, human aortic endothelial cells; IgG, immunoglobulin G; TNFα, tumor necrosis factor alpha.

    Article Snippet: Fresh adult human PBMCs (PCS‐800‐011; ATCC) were labeled with calcein acetomethoxy (AM) dye: PBMCs were pelleted at 240g for 10 minutes, resuspended in 1 mL of culture medium with 2.5 μmol/L of calcein AM from the kit, and incubated at 37°C (5% CO2 ) for 30 minutes.

    Techniques: Expressing, Immunofluorescence, Staining, Infection, Negative Control