Structured Review

3H Biomedical human pbmcs
IgGH is transported to the cell surface by HLA-DR. ( A ) The secreted forms of <t>IgGHs</t> cloned from human <t>PBMCs</t> that have different V regions were cotransfected with HLA-DRα, DRB1*04:04 (HLA-DR4), and GFP. Cell surface IgG on GFP-expressing cells was
Human Pbmcs, supplied by 3H Biomedical, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human pbmcs/product/3H Biomedical
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
human pbmcs - by Bioz Stars, 2020-09
92/100 stars

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1) Product Images from "Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility"

Article Title: Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.1401105111

IgGH is transported to the cell surface by HLA-DR. ( A ) The secreted forms of IgGHs cloned from human PBMCs that have different V regions were cotransfected with HLA-DRα, DRB1*04:04 (HLA-DR4), and GFP. Cell surface IgG on GFP-expressing cells was
Figure Legend Snippet: IgGH is transported to the cell surface by HLA-DR. ( A ) The secreted forms of IgGHs cloned from human PBMCs that have different V regions were cotransfected with HLA-DRα, DRB1*04:04 (HLA-DR4), and GFP. Cell surface IgG on GFP-expressing cells was

Techniques Used: Clone Assay, Expressing

Related Articles

Sequencing:

Article Title: Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility
Article Snippet: .. IgGHs were amplified from human PBMCs (3H Biomedical) using sense primers for V regions (5′-GTCTTGTCCCAGGTCACCTTGAAGGAG-3′ for clone 1 and 3, 5′-GCCCACTCCCAGGTGCAGCTGGTGCAG-3′ for clone 2, 5′-GTGCAGCTGGTGCAGTCTGGAGCAGAG-3′ for clone 4, 5′-GTCCAGTGTGAAGTGCAGCTGGTGGAG-3′ for clone 5), and antisense primer for constant region of secreted IgG (5′-TCATTTACCCGGAGACAGGGAG-3′) and cloned into pME18S expression vector containing a CD150 signal sequence. ..

Clone Assay:

Article Title: Transport of misfolded endoplasmic reticulum proteins to the cell surface by MHC class II molecules
Article Snippet: .. First, cDNAs for different HLA-class I and -class II alleles and Ii (accession No. ) were cloned from cDNAs prepared from pooled human PBMCs (3H Biomedical) or human cell lines. .. Dr P. Parham (Stanford University) kindly provided the HLA-Cw*04:01 (HLA-Cw4) vector.

Article Title: Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility
Article Snippet: .. cDNA for different HLA class II alleles, HLA-DMα ( DMA * 01:02 ), HLA-DMβ ( DMB * 01:01 ), Ii (accession no. NM_004355.2) were cloned from cDNA prepared from pooled human PBMCs (3H Biomedical) or human cell lines. .. Igα (accession No. ) and Igβ (accession No. NM_008339.2) were cloned from cDNA prepared from mouse spleen.

Article Title: Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility
Article Snippet: .. IgGHs were amplified from human PBMCs (3H Biomedical) using sense primers for V regions (5′-GTCTTGTCCCAGGTCACCTTGAAGGAG-3′ for clone 1 and 3, 5′-GCCCACTCCCAGGTGCAGCTGGTGCAG-3′ for clone 2, 5′-GTGCAGCTGGTGCAGTCTGGAGCAGAG-3′ for clone 4, 5′-GTCCAGTGTGAAGTGCAGCTGGTGGAG-3′ for clone 5), and antisense primer for constant region of secreted IgG (5′-TCATTTACCCGGAGACAGGGAG-3′) and cloned into pME18S expression vector containing a CD150 signal sequence. ..

Amplification:

Article Title: Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility
Article Snippet: .. IgGHs were amplified from human PBMCs (3H Biomedical) using sense primers for V regions (5′-GTCTTGTCCCAGGTCACCTTGAAGGAG-3′ for clone 1 and 3, 5′-GCCCACTCCCAGGTGCAGCTGGTGCAG-3′ for clone 2, 5′-GTGCAGCTGGTGCAGTCTGGAGCAGAG-3′ for clone 4, 5′-GTCCAGTGTGAAGTGCAGCTGGTGGAG-3′ for clone 5), and antisense primer for constant region of secreted IgG (5′-TCATTTACCCGGAGACAGGGAG-3′) and cloned into pME18S expression vector containing a CD150 signal sequence. ..

Expressing:

Article Title: Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility
Article Snippet: .. IgGHs were amplified from human PBMCs (3H Biomedical) using sense primers for V regions (5′-GTCTTGTCCCAGGTCACCTTGAAGGAG-3′ for clone 1 and 3, 5′-GCCCACTCCCAGGTGCAGCTGGTGCAG-3′ for clone 2, 5′-GTGCAGCTGGTGCAGTCTGGAGCAGAG-3′ for clone 4, 5′-GTCCAGTGTGAAGTGCAGCTGGTGGAG-3′ for clone 5), and antisense primer for constant region of secreted IgG (5′-TCATTTACCCGGAGACAGGGAG-3′) and cloned into pME18S expression vector containing a CD150 signal sequence. ..

Plasmid Preparation:

Article Title: Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility
Article Snippet: .. IgGHs were amplified from human PBMCs (3H Biomedical) using sense primers for V regions (5′-GTCTTGTCCCAGGTCACCTTGAAGGAG-3′ for clone 1 and 3, 5′-GCCCACTCCCAGGTGCAGCTGGTGCAG-3′ for clone 2, 5′-GTGCAGCTGGTGCAGTCTGGAGCAGAG-3′ for clone 4, 5′-GTCCAGTGTGAAGTGCAGCTGGTGGAG-3′ for clone 5), and antisense primer for constant region of secreted IgG (5′-TCATTTACCCGGAGACAGGGAG-3′) and cloned into pME18S expression vector containing a CD150 signal sequence. ..

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    3H Biomedical human pbmcs
    IgGH is transported to the cell surface by HLA-DR. ( A ) The secreted forms of <t>IgGHs</t> cloned from human <t>PBMCs</t> that have different V regions were cotransfected with HLA-DRα, DRB1*04:04 (HLA-DR4), and GFP. Cell surface IgG on GFP-expressing cells was
    Human Pbmcs, supplied by 3H Biomedical, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pbmcs/product/3H Biomedical
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    human pbmcs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    IgGH is transported to the cell surface by HLA-DR. ( A ) The secreted forms of IgGHs cloned from human PBMCs that have different V regions were cotransfected with HLA-DRα, DRB1*04:04 (HLA-DR4), and GFP. Cell surface IgG on GFP-expressing cells was

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility

    doi: 10.1073/pnas.1401105111

    Figure Lengend Snippet: IgGH is transported to the cell surface by HLA-DR. ( A ) The secreted forms of IgGHs cloned from human PBMCs that have different V regions were cotransfected with HLA-DRα, DRB1*04:04 (HLA-DR4), and GFP. Cell surface IgG on GFP-expressing cells was

    Article Snippet: IgGHs were amplified from human PBMCs (3H Biomedical) using sense primers for V regions (5′-GTCTTGTCCCAGGTCACCTTGAAGGAG-3′ for clone 1 and 3, 5′-GCCCACTCCCAGGTGCAGCTGGTGCAG-3′ for clone 2, 5′-GTGCAGCTGGTGCAGTCTGGAGCAGAG-3′ for clone 4, 5′-GTCCAGTGTGAAGTGCAGCTGGTGGAG-3′ for clone 5), and antisense primer for constant region of secreted IgG (5′-TCATTTACCCGGAGACAGGGAG-3′) and cloned into pME18S expression vector containing a CD150 signal sequence.

    Techniques: Clone Assay, Expressing