pbmc  (Lonza)


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  • 96
    Name:
    Human Peripheral Blood Mononuclear Cells
    Description:
    Mononuclear cells from human peripheral blood cryopreserved 10 million cells
    Catalog Number:
    4w-270
    Price:
    None
    Category:
    Primary and Stem Cells
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    Structured Review

    Lonza pbmc
    Functional analysis of MAIT cells in individuals of different ages. <t>PBMC</t> or CBMC from the US cohort were incubated overnight with M. smegmatis -infected A549 cells or uninfected A549 cells and then stained with the MR1-5-OP-RU or MR1/6FP tetramers, followed by staining with a live/dead discriminator and antibodies to TCRγδ, CD3, CD4, CD8, TRAV1-2, CD26 and CD161. <t>ICS</t> was then performed and the cells stained for TNF. A . Dot plots showing representative co-staining of live, TCRγδ - CD3 + TRAV1-2 + CD26 + CD161 + cells with MR1-5-OP-RU and TNF in M. smegmatis stimulated or unstimulated samples. The gating strategy is shown in Figure S3. Examples of the TNF response in a neonate, infant and adult are shown. B . Frequencies of functional, phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells that are MR1-5-OP-RU + or MR1-5-OP-RU - as a proportion of CD3 + TCRγδ - T cells. Horizontal lines depict the median and the error bars the 95% confidence interval. Wilcoxon-rank sum was used to test differences within the same cohort.
    Mononuclear cells from human peripheral blood cryopreserved 10 million cells
    https://www.bioz.com/result/pbmc/product/Lonza
    Average 96 stars, based on 264 article reviews
    Price from $9.99 to $1999.99
    pbmc - by Bioz Stars, 2020-09
    96/100 stars

    Images

    1) Product Images from "Postnatal Expansion, Maturation and Functionality of MR1T Cells in Humans"

    Article Title: Postnatal Expansion, Maturation and Functionality of MR1T Cells in Humans

    Journal: bioRxiv

    doi: 10.1101/2019.12.20.882746

    Functional analysis of MAIT cells in individuals of different ages. PBMC or CBMC from the US cohort were incubated overnight with M. smegmatis -infected A549 cells or uninfected A549 cells and then stained with the MR1-5-OP-RU or MR1/6FP tetramers, followed by staining with a live/dead discriminator and antibodies to TCRγδ, CD3, CD4, CD8, TRAV1-2, CD26 and CD161. ICS was then performed and the cells stained for TNF. A . Dot plots showing representative co-staining of live, TCRγδ - CD3 + TRAV1-2 + CD26 + CD161 + cells with MR1-5-OP-RU and TNF in M. smegmatis stimulated or unstimulated samples. The gating strategy is shown in Figure S3. Examples of the TNF response in a neonate, infant and adult are shown. B . Frequencies of functional, phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells that are MR1-5-OP-RU + or MR1-5-OP-RU - as a proportion of CD3 + TCRγδ - T cells. Horizontal lines depict the median and the error bars the 95% confidence interval. Wilcoxon-rank sum was used to test differences within the same cohort.
    Figure Legend Snippet: Functional analysis of MAIT cells in individuals of different ages. PBMC or CBMC from the US cohort were incubated overnight with M. smegmatis -infected A549 cells or uninfected A549 cells and then stained with the MR1-5-OP-RU or MR1/6FP tetramers, followed by staining with a live/dead discriminator and antibodies to TCRγδ, CD3, CD4, CD8, TRAV1-2, CD26 and CD161. ICS was then performed and the cells stained for TNF. A . Dot plots showing representative co-staining of live, TCRγδ - CD3 + TRAV1-2 + CD26 + CD161 + cells with MR1-5-OP-RU and TNF in M. smegmatis stimulated or unstimulated samples. The gating strategy is shown in Figure S3. Examples of the TNF response in a neonate, infant and adult are shown. B . Frequencies of functional, phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells that are MR1-5-OP-RU + or MR1-5-OP-RU - as a proportion of CD3 + TCRγδ - T cells. Horizontal lines depict the median and the error bars the 95% confidence interval. Wilcoxon-rank sum was used to test differences within the same cohort.

    Techniques Used: Functional Assay, Incubation, Infection, Staining

    Phenotypic analysis of functional MR1T cells at different ages. PBMC or CBMC from the US cohort were incubated overnight with M. smegmatis -infected A549 cells, uninfected A549 cells, or uninfected A549 cells and PMA/ionomycin. All cells were then stained with the MR1-5-OP-RU or MR1-6FP tetramers, followed by a live/dead discriminator and antibodies to TCRγδ, CD3, CD4, CD8, TRAV1-2, CD26 and CD161. ICS was then performed and the cells stained for TNF. Live, TCRγδ - CD3 + MR1-5-OP-RU + cells were gated and the TNF + percentage determined in both the M. smegmatis stimulated and unstimulated conditions. The gating strategy is shown in Figure S3. A . Dot plots showing TNF expression by live, TCRγδ - MR1-5-OP-RU + CD3 + cells in the same representative neonate, infant and adult as Figure 2A . B . Background subtracted frequencies of TNF + MR1-5-OP-RU + CD3 + cells in response to M. smegmatis -infected A549 cells as a percentage of total MR1/5-OP-RU + CD3 + cells are shown. Mann-Whitney u-tests were used to test differences between groups. C . Background subtracted frequencies of TNF + cells to PMA/ionomycin among different T cell subpopulations, 1) MR1T cells (CD3 + TCRγδ - MR1-5-OP-RU + cells); 2) γδ T cells (CD3 + TCRγδ + MR1-5-OP-RU - cells); 3) CD8 + T cells (CD3 + TCRγδ - MR1-5-OP-RU - CD8 + cells); and 4) CD4 + T cells (CD3 + TCRγδ - MR1-5-OP-RU - CD4 + cells). Wilcoxon-rank sum was used to test differences within the same cohort. D . and E . Frequencies of TNF + TRAV1-2 + MR1-5-OP-RU + CD3 + and TNF + TRAV1-2 - MR1-5-OP-RU + CD3 + cells in response to M. smegmatis infected A549 cells ( D ) or PMA/ionomycin stimulation ( E ) minus the background frequencies of TNF + TRAV1-2 + /- MR1-5-OP-RU + CD3 + cells. Wilcoxon-rank sum was used to test differences within the same cohort.
    Figure Legend Snippet: Phenotypic analysis of functional MR1T cells at different ages. PBMC or CBMC from the US cohort were incubated overnight with M. smegmatis -infected A549 cells, uninfected A549 cells, or uninfected A549 cells and PMA/ionomycin. All cells were then stained with the MR1-5-OP-RU or MR1-6FP tetramers, followed by a live/dead discriminator and antibodies to TCRγδ, CD3, CD4, CD8, TRAV1-2, CD26 and CD161. ICS was then performed and the cells stained for TNF. Live, TCRγδ - CD3 + MR1-5-OP-RU + cells were gated and the TNF + percentage determined in both the M. smegmatis stimulated and unstimulated conditions. The gating strategy is shown in Figure S3. A . Dot plots showing TNF expression by live, TCRγδ - MR1-5-OP-RU + CD3 + cells in the same representative neonate, infant and adult as Figure 2A . B . Background subtracted frequencies of TNF + MR1-5-OP-RU + CD3 + cells in response to M. smegmatis -infected A549 cells as a percentage of total MR1/5-OP-RU + CD3 + cells are shown. Mann-Whitney u-tests were used to test differences between groups. C . Background subtracted frequencies of TNF + cells to PMA/ionomycin among different T cell subpopulations, 1) MR1T cells (CD3 + TCRγδ - MR1-5-OP-RU + cells); 2) γδ T cells (CD3 + TCRγδ + MR1-5-OP-RU - cells); 3) CD8 + T cells (CD3 + TCRγδ - MR1-5-OP-RU - CD8 + cells); and 4) CD4 + T cells (CD3 + TCRγδ - MR1-5-OP-RU - CD4 + cells). Wilcoxon-rank sum was used to test differences within the same cohort. D . and E . Frequencies of TNF + TRAV1-2 + MR1-5-OP-RU + CD3 + and TNF + TRAV1-2 - MR1-5-OP-RU + CD3 + cells in response to M. smegmatis infected A549 cells ( D ) or PMA/ionomycin stimulation ( E ) minus the background frequencies of TNF + TRAV1-2 + /- MR1-5-OP-RU + CD3 + cells. Wilcoxon-rank sum was used to test differences within the same cohort.

    Techniques Used: Functional Assay, Incubation, Infection, Staining, Expressing, MANN-WHITNEY

    Frequencies of tetramer-defined MR1T cell and phenotypically defined MAIT cell populations in peripheral blood from individuals of different ages. PBMC or CBMC were stained with a live/dead discriminator, antibodies to CD3, CD4, CD8, TRAV1-2, CD26, CD161, and either the MR1-5-OP-RU or MR1-6FP tetramers. Live, CD3 + lymphocytes were gated and the frequencies of MR1-5-OP-RU + or TRAV1-2 + CD26 + CD161 + cells as a percentage of CD3 + lymphocytes were determined (gating strategy in Figure S2). A . Flow cytometry plots showing CD3 + T cell staining with MR1-5-OP-RU tetramer, MR1-6FP tetramer, TRAV1-2 or CD26/CD161 of samples from a representative US adult, infant and neonate. B . Frequencies of tetramer-defined (CD3 + MR1-5-OP-RU + ) and phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells in neonates, 10 week-old infants and adolescents from South Africa, neonates, 12 month-old infants and adults from the United States and infants (0-2 years old), children (2-5 years old) and adults from Uganda (all cohorts, n =10). Mann-Whitney u-tests were used to test differences between groups. Horizontal lines depict the median and the error bars the 95% confidence interval. C . Relative proportions of median phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells that are MR1-5-OP-RU + (Blue) and MR1-5-OP-RU - (Red shades) for each age group at each site. In each doughnut chart the MR1-5-OP-RU - population is further divided into CD8 + , CD8 + CD4 + , CD4 + , and CD8 - CD4 - populations.
    Figure Legend Snippet: Frequencies of tetramer-defined MR1T cell and phenotypically defined MAIT cell populations in peripheral blood from individuals of different ages. PBMC or CBMC were stained with a live/dead discriminator, antibodies to CD3, CD4, CD8, TRAV1-2, CD26, CD161, and either the MR1-5-OP-RU or MR1-6FP tetramers. Live, CD3 + lymphocytes were gated and the frequencies of MR1-5-OP-RU + or TRAV1-2 + CD26 + CD161 + cells as a percentage of CD3 + lymphocytes were determined (gating strategy in Figure S2). A . Flow cytometry plots showing CD3 + T cell staining with MR1-5-OP-RU tetramer, MR1-6FP tetramer, TRAV1-2 or CD26/CD161 of samples from a representative US adult, infant and neonate. B . Frequencies of tetramer-defined (CD3 + MR1-5-OP-RU + ) and phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells in neonates, 10 week-old infants and adolescents from South Africa, neonates, 12 month-old infants and adults from the United States and infants (0-2 years old), children (2-5 years old) and adults from Uganda (all cohorts, n =10). Mann-Whitney u-tests were used to test differences between groups. Horizontal lines depict the median and the error bars the 95% confidence interval. C . Relative proportions of median phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells that are MR1-5-OP-RU + (Blue) and MR1-5-OP-RU - (Red shades) for each age group at each site. In each doughnut chart the MR1-5-OP-RU - population is further divided into CD8 + , CD8 + CD4 + , CD4 + , and CD8 - CD4 - populations.

    Techniques Used: Staining, Flow Cytometry, MANN-WHITNEY

    2) Product Images from "Effect of Heat-Killed Lactobacillus paracasei KW3110 Ingestion on Ocular Disorders Caused by Visual Display Terminal (VDT) Loads: A Randomized, Double-Blind, Placebo-Controlled Parallel-Group Study"

    Article Title: Effect of Heat-Killed Lactobacillus paracasei KW3110 Ingestion on Ocular Disorders Caused by Visual Display Terminal (VDT) Loads: A Randomized, Double-Blind, Placebo-Controlled Parallel-Group Study

    Journal: Nutrients

    doi: 10.3390/nu10081058

    Effects of Lactobacillus paracasei KW3110 on cell viability under blue light exposure conditions. ( A ) Lactobacillus paracasei LW3110 activated human M2 macrophages. Human peripheral blood mononuclear cells (PBMC)-derived M2 macrophages were treated with 10 μg/mL L. paracasei KW3110 or untreated (control) and cultured for 24 h. Interleukin 10 (IL-10) concentration of the culture medium was measured by ELISAs. ( B ) The inhibitory effect of L. paracasei KW3110-stimulated human PBMC-derived M2 macrophage supernatants (KW3110-sup) or vehicle-treated supernatants (vehicle sup) on blue light-induced retinal cell death in ARPE-19 cells as measured by propidium iodide (PI) staining. The number of cells exhibiting PI fluorescence was counted. PI positive cells were expressed as the ratio of PI-positive to Hoechst 33342-positive cells. Assays were performed in triplicate wells. The data shows the mean ± SD for triplicate wells. ** p
    Figure Legend Snippet: Effects of Lactobacillus paracasei KW3110 on cell viability under blue light exposure conditions. ( A ) Lactobacillus paracasei LW3110 activated human M2 macrophages. Human peripheral blood mononuclear cells (PBMC)-derived M2 macrophages were treated with 10 μg/mL L. paracasei KW3110 or untreated (control) and cultured for 24 h. Interleukin 10 (IL-10) concentration of the culture medium was measured by ELISAs. ( B ) The inhibitory effect of L. paracasei KW3110-stimulated human PBMC-derived M2 macrophage supernatants (KW3110-sup) or vehicle-treated supernatants (vehicle sup) on blue light-induced retinal cell death in ARPE-19 cells as measured by propidium iodide (PI) staining. The number of cells exhibiting PI fluorescence was counted. PI positive cells were expressed as the ratio of PI-positive to Hoechst 33342-positive cells. Assays were performed in triplicate wells. The data shows the mean ± SD for triplicate wells. ** p

    Techniques Used: Derivative Assay, Cell Culture, Concentration Assay, Staining, Fluorescence

    3) Product Images from "Identification of 14-dehydroergosterol as a novel anti-inflammatory compound inducing tolerogenic dendritic cells"

    Article Title: Identification of 14-dehydroergosterol as a novel anti-inflammatory compound inducing tolerogenic dendritic cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-14446-1

    Effects of 14-DHE on human DCs inducing tolerogenic phenotype. CD14-positive cells in human PBMCs were cultured in medium containing 50 ng/ml of GM-CSF and 20 ng/ml of IL-4 in the presence of 14-DHE, and treated with 1 μg/ml of LPS and 100 ng/ml of IFN-γ on the sixth day of culture. ( A and B ) Expression of CD86 ( A ) and HLA-DR ( B ) in CD11c-positive cells was analyzed by flow cytometry. ( C ) IL-12p70 in the culture supernatant was quantified by ELISA. Data are means ± SEM of 3 wells per sample. * p
    Figure Legend Snippet: Effects of 14-DHE on human DCs inducing tolerogenic phenotype. CD14-positive cells in human PBMCs were cultured in medium containing 50 ng/ml of GM-CSF and 20 ng/ml of IL-4 in the presence of 14-DHE, and treated with 1 μg/ml of LPS and 100 ng/ml of IFN-γ on the sixth day of culture. ( A and B ) Expression of CD86 ( A ) and HLA-DR ( B ) in CD11c-positive cells was analyzed by flow cytometry. ( C ) IL-12p70 in the culture supernatant was quantified by ELISA. Data are means ± SEM of 3 wells per sample. * p

    Techniques Used: Cell Culture, Expressing, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay

    4) Product Images from "The novel reversible LSD1 inhibitor SP-2577 promotes anti-tumor immunity in SWItch/Sucrose-NonFermentable (SWI/SNF) complex mutated ovarian cancer"

    Article Title: The novel reversible LSD1 inhibitor SP-2577 promotes anti-tumor immunity in SWItch/Sucrose-NonFermentable (SWI/SNF) complex mutated ovarian cancer

    Journal: bioRxiv

    doi: 10.1101/2020.01.10.902528

    SP-2577 promotes lymphocytes infiltration in SCCOHT tumor organoids ( A ) Immune infiltration assay in SCCOHT organoids imaging analysis. COV434, BIN67 and SCCOHT-1 derived-organoids were incubated with conditioned medium pretreated with 3 μM SP-2577, SP-2513 or DMSO in the presence of RFP-tagged PBMCs. After 48h the levels of lymphocyte infiltration were measured by z-stack analysis by Cytation 5 imaging. P values for COV434=
    Figure Legend Snippet: SP-2577 promotes lymphocytes infiltration in SCCOHT tumor organoids ( A ) Immune infiltration assay in SCCOHT organoids imaging analysis. COV434, BIN67 and SCCOHT-1 derived-organoids were incubated with conditioned medium pretreated with 3 μM SP-2577, SP-2513 or DMSO in the presence of RFP-tagged PBMCs. After 48h the levels of lymphocyte infiltration were measured by z-stack analysis by Cytation 5 imaging. P values for COV434=

    Techniques Used: Imaging, Derivative Assay, Incubation

    5) Product Images from "Regulation of Inflammatory Gene Expression in PBMCs by Immunostimulatory Botanicals"

    Article Title: Regulation of Inflammatory Gene Expression in PBMCs by Immunostimulatory Botanicals

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0012561

    Temporal regulation of gene expression in PBMCs following Astragalus membranaceus treatment. PBMCs were treated with Astragalus extract for 3, 8 or 18 hours. Microarray analyzed gene data was plotted to compare gene expression differences between botanical and ethanol treatment of PBMCs (Part A). Each spot on the plots represents a specific gene. Only genes with present calls in both treatments are shown. The diagonal lines off the center represent 2-, 3-, 10-, and 30-fold levels of induction or repression of gene expression. Part B represents the roles and overall comparison of genes induced at 3, 8 and 18 hours post Astragalus treatment. Part C lists representative genes induced at 3, 8 and/or 18 hours post Astragalus treatment.
    Figure Legend Snippet: Temporal regulation of gene expression in PBMCs following Astragalus membranaceus treatment. PBMCs were treated with Astragalus extract for 3, 8 or 18 hours. Microarray analyzed gene data was plotted to compare gene expression differences between botanical and ethanol treatment of PBMCs (Part A). Each spot on the plots represents a specific gene. Only genes with present calls in both treatments are shown. The diagonal lines off the center represent 2-, 3-, 10-, and 30-fold levels of induction or repression of gene expression. Part B represents the roles and overall comparison of genes induced at 3, 8 and 18 hours post Astragalus treatment. Part C lists representative genes induced at 3, 8 and/or 18 hours post Astragalus treatment.

    Techniques Used: Expressing, Microarray

    Different PBMC isolates led to similar changes in gene expression following treatment with Astragalus membranaceus. Commercially available (PBMC set I) or fresh PBMCs (PBMC set II) were treated with Astragalus extract for 18 hours. Genes were sorted based on a threefold ( P
    Figure Legend Snippet: Different PBMC isolates led to similar changes in gene expression following treatment with Astragalus membranaceus. Commercially available (PBMC set I) or fresh PBMCs (PBMC set II) were treated with Astragalus extract for 18 hours. Genes were sorted based on a threefold ( P

    Techniques Used: Expressing

    Astragalus membranaceus treatment of PBMCs led to monocyte maturation. Part A) PBMCs (upper panels) or the monocyte cell line THP-1 (lower panel) were untreated, treated with ethanol (25%), or treated with Astragalus extract for 18 hours. Following treatment, unattached cells were removed and the remaining attached cells photographed. Part B) PBMCs were untreated or treated with Astragalus extract or PMA for 18 hours. After 18 hours, the cell culture media was removed and cells pelleted by centrifugation. The cell-free culture media was added to THP-1 cells for an additional 24 hours. Following treatment, unattached cells were removed and the remaining attached cells photographed. Part C) Cell-free media from mock-, Astragalus, or PMA-treated PBMCs was added to THP-1 cells and incubated in uncoated plastic dishes. After 48 hours, cells were washed and stained with fluorochrome-conjugated antibodies specific for CD14 and CD11b followed by flow cytometry analysis. Part D) Cell-free media obtained from PBMCs isolated from two patients were used to treat THP-1 cells and analyzed for CD14 and CD11b expression as in Part C. Values indicated represent total CD14 cells, total CD11b cells and CD14/CD11b double-positive cells. Data was normalized to mock-treated samples.
    Figure Legend Snippet: Astragalus membranaceus treatment of PBMCs led to monocyte maturation. Part A) PBMCs (upper panels) or the monocyte cell line THP-1 (lower panel) were untreated, treated with ethanol (25%), or treated with Astragalus extract for 18 hours. Following treatment, unattached cells were removed and the remaining attached cells photographed. Part B) PBMCs were untreated or treated with Astragalus extract or PMA for 18 hours. After 18 hours, the cell culture media was removed and cells pelleted by centrifugation. The cell-free culture media was added to THP-1 cells for an additional 24 hours. Following treatment, unattached cells were removed and the remaining attached cells photographed. Part C) Cell-free media from mock-, Astragalus, or PMA-treated PBMCs was added to THP-1 cells and incubated in uncoated plastic dishes. After 48 hours, cells were washed and stained with fluorochrome-conjugated antibodies specific for CD14 and CD11b followed by flow cytometry analysis. Part D) Cell-free media obtained from PBMCs isolated from two patients were used to treat THP-1 cells and analyzed for CD14 and CD11b expression as in Part C. Values indicated represent total CD14 cells, total CD11b cells and CD14/CD11b double-positive cells. Data was normalized to mock-treated samples.

    Techniques Used: Cell Culture, Centrifugation, Incubation, Staining, Flow Cytometry, Cytometry, Isolation, Expressing

    Host gene expression regulated by Astragalus membranaceus treatment of PBMCs. Genes were sorted based on a threefold ( P
    Figure Legend Snippet: Host gene expression regulated by Astragalus membranaceus treatment of PBMCs. Genes were sorted based on a threefold ( P

    Techniques Used: Expressing

    Transcriptional profiling of M1/M2 macrophage polarization induced by Astragalus membranaceus. Human genes involved in defining M1/M2 polarization are shown [47] . The transcriptional regulatory effect of each gene following treatment of PBMCs with Astragalus extract is indicated (‘Expression’ column), with positive values representing increased expression, and negative values representing repressed expression. The ‘X’ (in the ‘M1 like’ or M2 like' column) indicates that the change in gene expression (induced or repressed) following Astragalus treatment was similar to that previously observed following monocyte maturation to either an M1-like or M2-like macrophage polarization.
    Figure Legend Snippet: Transcriptional profiling of M1/M2 macrophage polarization induced by Astragalus membranaceus. Human genes involved in defining M1/M2 polarization are shown [47] . The transcriptional regulatory effect of each gene following treatment of PBMCs with Astragalus extract is indicated (‘Expression’ column), with positive values representing increased expression, and negative values representing repressed expression. The ‘X’ (in the ‘M1 like’ or M2 like' column) indicates that the change in gene expression (induced or repressed) following Astragalus treatment was similar to that previously observed following monocyte maturation to either an M1-like or M2-like macrophage polarization.

    Techniques Used: Expressing

    Scatter plot representation of botanical extract regulation of gene expression. Microarray analyzed gene data was plotted to compare gene expression differences between botanical and ethanol treatment of PBMCs (Part A). Each spot on the plots represents a specific gene. Only genes with present calls in both treatments are shown. The diagonal lines off the center represent 2-, 3-, 10-, and 30-fold levels of induction or repression of gene expression. Part B illustrates comparative analysis between different botanical treatments of PBMCs.
    Figure Legend Snippet: Scatter plot representation of botanical extract regulation of gene expression. Microarray analyzed gene data was plotted to compare gene expression differences between botanical and ethanol treatment of PBMCs (Part A). Each spot on the plots represents a specific gene. Only genes with present calls in both treatments are shown. The diagonal lines off the center represent 2-, 3-, 10-, and 30-fold levels of induction or repression of gene expression. Part B illustrates comparative analysis between different botanical treatments of PBMCs.

    Techniques Used: Expressing, Microarray

    6) Product Images from "A novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling"

    Article Title: A novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.12102

    Leucocyte adhesion inhibition assay. Human anti-vascular cell adhesion molecule (VCAM)-1 mAb effectively inhibited leucocytes from binding to recombinant VCAM-1. U937 cell ( A ) and EoL-1 cell ( B ) adhesion was inhibited with a 0.1 μg/well concentration. In CD4 +  T cell ( C ), 64.5 ± 3.3% inhibition was obtained at 10 μg/well concentration. Human anti-VCAM-1 mAb effectively inhibited U937 cell from binding to a VCAM-1 expressing HUVEC mono-layer in a dose-dependent manner ( D ). All data showed as mean ± SEM of three independent assays.
    Figure Legend Snippet: Leucocyte adhesion inhibition assay. Human anti-vascular cell adhesion molecule (VCAM)-1 mAb effectively inhibited leucocytes from binding to recombinant VCAM-1. U937 cell ( A ) and EoL-1 cell ( B ) adhesion was inhibited with a 0.1 μg/well concentration. In CD4 + T cell ( C ), 64.5 ± 3.3% inhibition was obtained at 10 μg/well concentration. Human anti-VCAM-1 mAb effectively inhibited U937 cell from binding to a VCAM-1 expressing HUVEC mono-layer in a dose-dependent manner ( D ). All data showed as mean ± SEM of three independent assays.

    Techniques Used: Inhibition, Binding Assay, Recombinant, Concentration Assay, Expressing

    Related Articles

    Centrifugation:

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    Article Snippet: .. Culture and Activation of Human Peripheral Blood Mononuclear Cells (PBMCs) and Isolation of CD8+ T cells PBMCs were isolated from venous blood of healthy donors using lymphocyte separation medium (Lonza Biologics, Portsmouth, NH) and density centrifugation. .. PBMCs were then cultured at 1×106 cells mL of RPMI 1640, 10% heat-inactivated fetal bovine serum (FBS), 200 mM L-glutamine, and 1% penicillin/streptomycin (Lonza Biologics, Portsmouth, NH).

    In Vitro:

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    Co-Culture Assay:

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    Isolation:

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    Article Snippet: .. Human peripheral blood mononuclear cells were isolated following standard procedure ( ) and cultured for 16 h in RPMI 1640 medium (Lonza) with 10% of FCS, 2 mM Glutamax, and 100 U/ml penicillin–streptomycin (Life Technologies). .. After monocyte adherence, cells were washed and primed for 4 h with LPS (10 ng/ml), and then cells were washed or not with physiological buffer and incubated in the same buffer at 37°C with 3 mM of ATP for 20 min.

    Article Title: Porcupine Is Not Required for the Production of the Majority of Wnts from Primary Human Astrocytes and CD8+ T Cells
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    Article Title: Kv1.3 Channel Blockade Modulates the Effector Function of B Cells in Granulomatosis with Polyangiitis
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    Cell Culture:

    Article Title: P2X7 Receptor Induces Tumor Necrosis Factor-α Converting Enzyme Activation and Release to Boost TNF-α Production
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    Activation Assay:

    Article Title: Porcupine Is Not Required for the Production of the Majority of Wnts from Primary Human Astrocytes and CD8+ T Cells
    Article Snippet: .. Culture and Activation of Human Peripheral Blood Mononuclear Cells (PBMCs) and Isolation of CD8+ T cells PBMCs were isolated from venous blood of healthy donors using lymphocyte separation medium (Lonza Biologics, Portsmouth, NH) and density centrifugation. .. PBMCs were then cultured at 1×106 cells mL of RPMI 1640, 10% heat-inactivated fetal bovine serum (FBS), 200 mM L-glutamine, and 1% penicillin/streptomycin (Lonza Biologics, Portsmouth, NH).

    other:

    Article Title: High expression of IDO1 and TGF-β1 during recurrence and post infection clearance with Chlamydia trachomatis, are independent of host IFN-γ response
    Article Snippet: In addition, control plates with either ECC1 cells alone or human PBMCs alone were used.

    Infection:

    Article Title: High expression of IDO1 and TGF-β1 during recurrence and post infection clearance with Chlamydia trachomatis, are independent of host IFN-γ response
    Article Snippet: .. In addition, infection of human PBMCs alone with live C. trachomatis , without the azithromycin treatment, resulted in significantly reduced, yet apparent infectivity (p < 0.0001). .. The number of chlamydial IFUs measured from ECC1 and PBMCs co-culture was significantly reduced in comparison to infected ECC1 culture alone (p < 0.0001) by almost two logs.

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    Lonza cd4 t cells
    Leucocyte adhesion inhibition assay. Human anti-vascular cell adhesion molecule (VCAM)-1 mAb effectively inhibited leucocytes from binding to recombinant VCAM-1. U937 cell ( A ) and EoL-1 cell ( B ) adhesion was inhibited with a 0.1 μg/well concentration. In CD4 +  T cell ( C ), 64.5 ± 3.3% inhibition was obtained at 10 μg/well concentration. Human anti-VCAM-1 mAb effectively inhibited U937 cell from binding to a VCAM-1 expressing HUVEC mono-layer in a dose-dependent manner ( D ). All data showed as mean ± SEM of three independent assays.
    Cd4 T Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Leucocyte adhesion inhibition assay. Human anti-vascular cell adhesion molecule (VCAM)-1 mAb effectively inhibited leucocytes from binding to recombinant VCAM-1. U937 cell ( A ) and EoL-1 cell ( B ) adhesion was inhibited with a 0.1 μg/well concentration. In CD4 +  T cell ( C ), 64.5 ± 3.3% inhibition was obtained at 10 μg/well concentration. Human anti-VCAM-1 mAb effectively inhibited U937 cell from binding to a VCAM-1 expressing HUVEC mono-layer in a dose-dependent manner ( D ). All data showed as mean ± SEM of three independent assays.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: A novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling

    doi: 10.1111/jcmm.12102

    Figure Lengend Snippet: Leucocyte adhesion inhibition assay. Human anti-vascular cell adhesion molecule (VCAM)-1 mAb effectively inhibited leucocytes from binding to recombinant VCAM-1. U937 cell ( A ) and EoL-1 cell ( B ) adhesion was inhibited with a 0.1 μg/well concentration. In CD4 + T cell ( C ), 64.5 ± 3.3% inhibition was obtained at 10 μg/well concentration. Human anti-VCAM-1 mAb effectively inhibited U937 cell from binding to a VCAM-1 expressing HUVEC mono-layer in a dose-dependent manner ( D ). All data showed as mean ± SEM of three independent assays.

    Article Snippet: Meanwhile, human leucocytes—U937 cells (CRL-1593.2; ATCC, Manassas, VA, USA), EoL-1 cells (94042252; ECACC, Salisbury, UK) or CD4+ T cells (isolated from human peripheral blood mononuclear cells, CC-2702, Lonza, Basel, Swiss)—were stained with 5 μM carboxyfluorescein diacetate succinimidyl ester (CFSE) (C34554; Invitrogen, Carlsbad, CA, USA).

    Techniques: Inhibition, Binding Assay, Recombinant, Concentration Assay, Expressing