human nonsmall cell lung cancer cell line  (ATCC)


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    ATCC human nonsmall cell lung cancer cell line
    Human Nonsmall Cell Lung Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human nonsmall cell lung cancer cell line a549  (ATCC)


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    ATCC human nonsmall cell lung cancer cell line a549
    Human Nonsmall Cell Lung Cancer Cell Line A549, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human nonsmall cell lung cancer cell line  (ATCC)


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    ATCC human nonsmall cell lung cancer cell line
    Human Nonsmall Cell Lung Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a549 nonsmall cell lung cancer cells line  (ATCC)


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    ATCC a549 nonsmall cell lung cancer cells line
    (a) Immunocytochemical staining and (b) protein level of EGFR expression in LO2 cells, HeLa cells, and <t>A549</t> cells. Scale bar = 50 µ m. (c) FL images of A549 cells after 4 h of incubation with free ICG, ICG-LPs, GE11-ICG-LPs, and GE11-ICG-LPs with free GE11 peptide or anti-EGFR antibody blocking. DAPI (blue) counterstains cell nuclei. Scale bar = 50 µ m. (d) The semiquantitative analysis of fluorescence intensity in (c) was determined by ‘Image J' software. Data are expressed as mean ± SD ( n = 3). (e) FL images of A549 cells incubated with GE11-CUR/ICG-LPs after laser irradiation (1 W·cm −2 ) for different times. Scale bar = 50 µ m.
    A549 Nonsmall Cell Lung Cancer Cells Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy"

    Article Title: GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

    Journal: Bioinorganic Chemistry and Applications

    doi: 10.1155/2021/5534870

    (a) Immunocytochemical staining and (b) protein level of EGFR expression in LO2 cells, HeLa cells, and A549 cells. Scale bar = 50 µ m. (c) FL images of A549 cells after 4 h of incubation with free ICG, ICG-LPs, GE11-ICG-LPs, and GE11-ICG-LPs with free GE11 peptide or anti-EGFR antibody blocking. DAPI (blue) counterstains cell nuclei. Scale bar = 50 µ m. (d) The semiquantitative analysis of fluorescence intensity in (c) was determined by ‘Image J' software. Data are expressed as mean ± SD ( n = 3). (e) FL images of A549 cells incubated with GE11-CUR/ICG-LPs after laser irradiation (1 W·cm −2 ) for different times. Scale bar = 50 µ m.
    Figure Legend Snippet: (a) Immunocytochemical staining and (b) protein level of EGFR expression in LO2 cells, HeLa cells, and A549 cells. Scale bar = 50 µ m. (c) FL images of A549 cells after 4 h of incubation with free ICG, ICG-LPs, GE11-ICG-LPs, and GE11-ICG-LPs with free GE11 peptide or anti-EGFR antibody blocking. DAPI (blue) counterstains cell nuclei. Scale bar = 50 µ m. (d) The semiquantitative analysis of fluorescence intensity in (c) was determined by ‘Image J' software. Data are expressed as mean ± SD ( n = 3). (e) FL images of A549 cells incubated with GE11-CUR/ICG-LPs after laser irradiation (1 W·cm −2 ) for different times. Scale bar = 50 µ m.

    Techniques Used: Staining, Expressing, Incubation, Blocking Assay, Fluorescence, Software, Irradiation

    (a), (b) Cytotoxicity and phototoxicity of free CUR/ICG, CUR/ICG-LPs and GE11-CUR/ICG-LPs under the NIR laser irradiation (808 nm) of 1 W·cm −2 for 5 min at different concentrations on A549 cells after 24 h incubation. (c) Fluorescence images of A549 cells stained with calcein-AM (green) after different treatments. Scale bar = 100 μ m. (d) Synergistic effect of photo- and chemotherapy based on GE11-CUR/ICG-LPs. Cytotoxicity of GE11-ICG-LPs, GE11-CUR-LPs, and GE11-CUR/ICG-LPs with or without laser exposure was performed by MTT assays. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
    Figure Legend Snippet: (a), (b) Cytotoxicity and phototoxicity of free CUR/ICG, CUR/ICG-LPs and GE11-CUR/ICG-LPs under the NIR laser irradiation (808 nm) of 1 W·cm −2 for 5 min at different concentrations on A549 cells after 24 h incubation. (c) Fluorescence images of A549 cells stained with calcein-AM (green) after different treatments. Scale bar = 100 μ m. (d) Synergistic effect of photo- and chemotherapy based on GE11-CUR/ICG-LPs. Cytotoxicity of GE11-ICG-LPs, GE11-CUR-LPs, and GE11-CUR/ICG-LPs with or without laser exposure was performed by MTT assays. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

    Techniques Used: Irradiation, Incubation, Fluorescence, Staining

    (a) Effects of IC-GLPs on the caspase-3 activity in A549 cells, data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001. (b) Intracellular ROS detection by the DCFH-DA fluorescence staining. Scale bar = 50 μ m. (c) Cytoskeletal microtubulin (red) expression in A549 cells after different treatments with laser irradiation. DAPI (blue) counterstains cell nuclei. Scale bar = 10 μ m.
    Figure Legend Snippet: (a) Effects of IC-GLPs on the caspase-3 activity in A549 cells, data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001. (b) Intracellular ROS detection by the DCFH-DA fluorescence staining. Scale bar = 50 μ m. (c) Cytoskeletal microtubulin (red) expression in A549 cells after different treatments with laser irradiation. DAPI (blue) counterstains cell nuclei. Scale bar = 10 μ m.

    Techniques Used: Activity Assay, Fluorescence, Staining, Expressing, Irradiation

    (a) Representative western blot and (b) quantification of Bcl-2 and Bax expression in A549 cells following treatment with PBS, free CUR/ICG, CUR/ICG-LPs, and GE11-CUR/ICG-LPs under 808 nm laser irradiation for 5 min. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05 and ∗∗ P < 0.01. (c) Representative western blot and (d) quantification of PI3K, p-PI3K, Akt, and p-Akt expression in A549 cells following different treatments with laser irradiation. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.
    Figure Legend Snippet: (a) Representative western blot and (b) quantification of Bcl-2 and Bax expression in A549 cells following treatment with PBS, free CUR/ICG, CUR/ICG-LPs, and GE11-CUR/ICG-LPs under 808 nm laser irradiation for 5 min. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05 and ∗∗ P < 0.01. (c) Representative western blot and (d) quantification of PI3K, p-PI3K, Akt, and p-Akt expression in A549 cells following different treatments with laser irradiation. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

    Techniques Used: Western Blot, Expressing, Irradiation

    human nonsmall cell lung cancer cell lines  (ATCC)


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    ATCC human nonsmall cell lung cancer cell lines
    CMIP is upregulated in LUAD and associated with poor prognosis. (a) Immunohistochemical tissue microarray image (HPA) of CMIP protein. (b) qRT-PCR detection of CMIP mRNA levels in normal lung tissue and LUAD tissue ( n = 20); (c) Western blot detection of CMIP protein expression in normal lung tissue and LUAD tissue ( n = 3); (d) mRNA levels of CMIP detected by qRT-PCR in BEAS-2B, <t>A549,</t> <t>H460,</t> and <t>H1299</t> cells ( n = 3); (e) OS and PFS in LUAD patients obtained from the Kaplan–Meier plotter online database; ∗∗ P < 0.01 vs. (Normal group or BEAS-2B group).
    Human Nonsmall Cell Lung Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "CMaf-Inducing Protein Promotes LUAD Proliferation and Metastasis by Activating the MAPK/ERK Pathway"

    Article Title: CMaf-Inducing Protein Promotes LUAD Proliferation and Metastasis by Activating the MAPK/ERK Pathway

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2022/2501846

    CMIP is upregulated in LUAD and associated with poor prognosis. (a) Immunohistochemical tissue microarray image (HPA) of CMIP protein. (b) qRT-PCR detection of CMIP mRNA levels in normal lung tissue and LUAD tissue ( n = 20); (c) Western blot detection of CMIP protein expression in normal lung tissue and LUAD tissue ( n = 3); (d) mRNA levels of CMIP detected by qRT-PCR in BEAS-2B, A549, H460, and H1299 cells ( n = 3); (e) OS and PFS in LUAD patients obtained from the Kaplan–Meier plotter online database; ∗∗ P < 0.01 vs. (Normal group or BEAS-2B group).
    Figure Legend Snippet: CMIP is upregulated in LUAD and associated with poor prognosis. (a) Immunohistochemical tissue microarray image (HPA) of CMIP protein. (b) qRT-PCR detection of CMIP mRNA levels in normal lung tissue and LUAD tissue ( n = 20); (c) Western blot detection of CMIP protein expression in normal lung tissue and LUAD tissue ( n = 3); (d) mRNA levels of CMIP detected by qRT-PCR in BEAS-2B, A549, H460, and H1299 cells ( n = 3); (e) OS and PFS in LUAD patients obtained from the Kaplan–Meier plotter online database; ∗∗ P < 0.01 vs. (Normal group or BEAS-2B group).

    Techniques Used: Immunohistochemical staining, Microarray, Quantitative RT-PCR, Western Blot, Expressing

    CMIP overexpression promotes the proliferation of H460 cells. (a/b) The mRNA levels of CMIP in H460 (a) and H1299 (b) cells after transfection were detected by qRT-PCR; (c/d) MTT assay was used to detect the difference of CMIP expression after transfection on H460 (c) and H1299 (d) Effect of cell viability. (e/f) Cell colony formation assay to assess the effect of CMIP expression on the proliferation of H460 (e) and H1299 (f) cells after transfection. ∗∗ P < 0.01 vs. (siNC group or vector group).
    Figure Legend Snippet: CMIP overexpression promotes the proliferation of H460 cells. (a/b) The mRNA levels of CMIP in H460 (a) and H1299 (b) cells after transfection were detected by qRT-PCR; (c/d) MTT assay was used to detect the difference of CMIP expression after transfection on H460 (c) and H1299 (d) Effect of cell viability. (e/f) Cell colony formation assay to assess the effect of CMIP expression on the proliferation of H460 (e) and H1299 (f) cells after transfection. ∗∗ P < 0.01 vs. (siNC group or vector group).

    Techniques Used: Over Expression, Transfection, Quantitative RT-PCR, MTT Assay, Expressing, Colony Assay, Plasmid Preparation

    CMIP overexpression promotes H460 cell migration and invasion. (a/b) The wound healing assay was used to analyze the effect of CMIP expression on the migration of H460 (a) and H1299 (b) cells after transfection. (c/d) The effect of CMIP expression on H460 (c) and H1299 (d) cell invasion after transfection was assessed using Transwell analysis. ∗∗ P < 0.01 vs. (siNC group or vector group).
    Figure Legend Snippet: CMIP overexpression promotes H460 cell migration and invasion. (a/b) The wound healing assay was used to analyze the effect of CMIP expression on the migration of H460 (a) and H1299 (b) cells after transfection. (c/d) The effect of CMIP expression on H460 (c) and H1299 (d) cell invasion after transfection was assessed using Transwell analysis. ∗∗ P < 0.01 vs. (siNC group or vector group).

    Techniques Used: Over Expression, Migration, Wound Healing Assay, Expressing, Transfection, Plasmid Preparation

    CMIP overexpression promotes activation of the MAPK/ERK pathway. (a, b) Western blot detection of P38, ERK, p-P38 and p-ERK protein expression, and the relative expression of p-P38/P38 and p-ERK/ERK in H1299 cells. (c, d) Western blot detection of P38, ERK, p-P38 and p-ERK protein expression, and the relative expression of p-P38/P38 and p-ERK/ERK in H460 cells. ∗∗ P < 0.01 vs. (siNC group or vector group).
    Figure Legend Snippet: CMIP overexpression promotes activation of the MAPK/ERK pathway. (a, b) Western blot detection of P38, ERK, p-P38 and p-ERK protein expression, and the relative expression of p-P38/P38 and p-ERK/ERK in H1299 cells. (c, d) Western blot detection of P38, ERK, p-P38 and p-ERK protein expression, and the relative expression of p-P38/P38 and p-ERK/ERK in H460 cells. ∗∗ P < 0.01 vs. (siNC group or vector group).

    Techniques Used: Over Expression, Activation Assay, Western Blot, Expressing, Plasmid Preparation

    CMIP promotes H460 cell proliferation, migration, and invasion by activating the MAPK/ERK pathway. (a) MTT assay to detect cell viability in each group; (b) cell clone formation assay to detect the number of cell clones in each group; (c) wound healing assay to detect cell migration in each group; (d) Transwell analysis to detect cell invasion ability of each group. ∗ P < 0.05 and ∗∗ P < 0.01 vs. vector group, ## P < 0.01 vs. OE-CMIP group.
    Figure Legend Snippet: CMIP promotes H460 cell proliferation, migration, and invasion by activating the MAPK/ERK pathway. (a) MTT assay to detect cell viability in each group; (b) cell clone formation assay to detect the number of cell clones in each group; (c) wound healing assay to detect cell migration in each group; (d) Transwell analysis to detect cell invasion ability of each group. ∗ P < 0.05 and ∗∗ P < 0.01 vs. vector group, ## P < 0.01 vs. OE-CMIP group.

    Techniques Used: Migration, MTT Assay, Tube Formation Assay, Clone Assay, Wound Healing Assay, Plasmid Preparation

    human nonsmall cell lung cancer cell line  (ATCC)


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    ATCC human nonsmall cell lung cancer cell line
    Human Nonsmall Cell Lung Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human nonsmall cell lung cancer nsclc cell lines h1299  (ATCC)


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    ATCC human nonsmall cell lung cancer nsclc cell lines h1299
    Effect of sirtinol on cellular proliferation of <t>H1299</t> cells. H1299 cells treated with different concentrations (5, 10, 20 and 50 μ M) of sirtinol for 24 h and 48 h, respectively. The cell survival was determined by the trypan blue staining assay combined with the Countess Automated Cell Counter. ** P < 0.001 against vehicle control.
    Human Nonsmall Cell Lung Cancer Nsclc Cell Lines H1299, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis"

    Article Title: The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis

    Journal: The Scientific World Journal

    doi: 10.1155/2014/937051

    Effect of sirtinol on cellular proliferation of H1299 cells. H1299 cells treated with different concentrations (5, 10, 20 and 50 μ M) of sirtinol for 24 h and 48 h, respectively. The cell survival was determined by the trypan blue staining assay combined with the Countess Automated Cell Counter. ** P < 0.001 against vehicle control.
    Figure Legend Snippet: Effect of sirtinol on cellular proliferation of H1299 cells. H1299 cells treated with different concentrations (5, 10, 20 and 50 μ M) of sirtinol for 24 h and 48 h, respectively. The cell survival was determined by the trypan blue staining assay combined with the Countess Automated Cell Counter. ** P < 0.001 against vehicle control.

    Techniques Used: Staining

    Sirtinol inhibits the colony formation of lung cancer cells. H1299 cells were treated with different concentrations (5, 10, 20 and 50 μ M) of sirtinol for 15 days, respectively. Afterwards, the cells were glutaraldehyde-fixed and stained with Giemsa stain for 1 h. (a) The colony formation analysis of H1299 cells. (b) The quantification analysis of the colony diameter. Data are represented as mean ± SD ( n = 3). * P < 0.01 compared with the vehicle control.
    Figure Legend Snippet: Sirtinol inhibits the colony formation of lung cancer cells. H1299 cells were treated with different concentrations (5, 10, 20 and 50 μ M) of sirtinol for 15 days, respectively. Afterwards, the cells were glutaraldehyde-fixed and stained with Giemsa stain for 1 h. (a) The colony formation analysis of H1299 cells. (b) The quantification analysis of the colony diameter. Data are represented as mean ± SD ( n = 3). * P < 0.01 compared with the vehicle control.

    Techniques Used: Staining, Giemsa Stain

    Sirtinol induces apoptosis of H1299 cells. Cells were treated with indicated concentrations of sirtinol and stained with Annexin-V and PI at 24 h, respectively. (a) Flow cytometry profiling represents the results of Annexin-V-FITC staining. (b) The quantificative analysis of cell apoptosis. Different letter notations indicate the statistical significance between sirtinol treatment and vehicle ( a versus b and a versus c indicate the P < 0.05 and 0.001, resp.).
    Figure Legend Snippet: Sirtinol induces apoptosis of H1299 cells. Cells were treated with indicated concentrations of sirtinol and stained with Annexin-V and PI at 24 h, respectively. (a) Flow cytometry profiling represents the results of Annexin-V-FITC staining. (b) The quantificative analysis of cell apoptosis. Different letter notations indicate the statistical significance between sirtinol treatment and vehicle ( a versus b and a versus c indicate the P < 0.05 and 0.001, resp.).

    Techniques Used: Staining, Flow Cytometry

    The effect of sirtinol on cell cycle distribution of lung cancer cells. H1299 cells treated with indicated concentrations (from 5 to 50 μ M) of sirtinol for 24 h, respectively. Cells were stained with PI and detected the cell cycle distribution by flow cytometry. (a) Flow cytometry profile represents PI staining in x -axis and cell number in y -axis. (b) The quantitative analysis of cell cycle distribution. Different letter notations indicate the statistical significance between drug treatment and vehicle ( a versus b and a versus c indicate the P < 0.05 and 0.001, resp.).
    Figure Legend Snippet: The effect of sirtinol on cell cycle distribution of lung cancer cells. H1299 cells treated with indicated concentrations (from 5 to 50 μ M) of sirtinol for 24 h, respectively. Cells were stained with PI and detected the cell cycle distribution by flow cytometry. (a) Flow cytometry profile represents PI staining in x -axis and cell number in y -axis. (b) The quantitative analysis of cell cycle distribution. Different letter notations indicate the statistical significance between drug treatment and vehicle ( a versus b and a versus c indicate the P < 0.05 and 0.001, resp.).

    Techniques Used: Staining, Flow Cytometry

    Modulation of protein levels in NSCLC H1299 cells after sirtinol treatment. H1299 cells treated with indicated concentrations (10, 20, and 50 μ M) of sirtinol for 24 h, respectively. The results of Western blot of Sirt1 nonhistone target protein, including FoxO3a, Akt phosphorylation, and β -catenin. β -Actin as an internal control.
    Figure Legend Snippet: Modulation of protein levels in NSCLC H1299 cells after sirtinol treatment. H1299 cells treated with indicated concentrations (10, 20, and 50 μ M) of sirtinol for 24 h, respectively. The results of Western blot of Sirt1 nonhistone target protein, including FoxO3a, Akt phosphorylation, and β -catenin. β -Actin as an internal control.

    Techniques Used: Western Blot

    Possible model of sirtinol-induced antiproliferation and apoptosis in lung cancer cells. Sirtinol downregulates the activation of prosurvival Akt serine/threonine kinase and the protein level of β -catenin, a proliferation-associated transcription factor, insulting in the cell cycle G 1 -phase accumulation and the growth arrest. On the contrary, sirtinol treatment causes the upregulation of the proapoptotic transcription factor FoxO3a, a target of both Akt signaling and Sirt1. This may render H1299 cells more sensitive to apoptosis. Finally, sirtinol induces the apoptosis of lung cancer cells.
    Figure Legend Snippet: Possible model of sirtinol-induced antiproliferation and apoptosis in lung cancer cells. Sirtinol downregulates the activation of prosurvival Akt serine/threonine kinase and the protein level of β -catenin, a proliferation-associated transcription factor, insulting in the cell cycle G 1 -phase accumulation and the growth arrest. On the contrary, sirtinol treatment causes the upregulation of the proapoptotic transcription factor FoxO3a, a target of both Akt signaling and Sirt1. This may render H1299 cells more sensitive to apoptosis. Finally, sirtinol induces the apoptosis of lung cancer cells.

    Techniques Used: Activation Assay

    human nonsmall cell lung cancer cells  (ATCC)


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    ATCC human nonsmall cell lung cancer cells
    Antiproliferation activity in vitro, antitumor activity in vivo, and toxicity of ICCA: ( A ) cell viabilities of ICCA-treated <t>A549,</t> 95D, K562, and S180 cells, n=6; ( B ) tumor weight of S180 mice orally treated with 5 μmol/kg/day ICCA for 9 consecutive days, n=12; ( C ) Cr of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( D ) ALT of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( E ) AST of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( F ) BUN of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6. Abbreviations: 95D, <t>nonsmall</t> cell lung cancer cell line; ALT, alanine transaminase; AST, aspartate transaminase; BUN, blood urea nitrogen; CMCNa, carboxymethyl cellulose sodium; Cr, creatinine; DOX, doxorubicin; ICCA, 1-(4-isopropylphenyl)-β-carboline-3-carboxylic acid; K562, human myeloid leukemia; S180, ascites tumor cells; Kar, Karmen.
    Human Nonsmall Cell Lung Cancer Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Design and development of ICCA as a dual inhibitor of GPIIb/IIIa and P-selectin receptors"

    Article Title: Design and development of ICCA as a dual inhibitor of GPIIb/IIIa and P-selectin receptors

    Journal: Drug Design, Development and Therapy

    doi: 10.2147/DDDT.S169238

    Antiproliferation activity in vitro, antitumor activity in vivo, and toxicity of ICCA: ( A ) cell viabilities of ICCA-treated A549, 95D, K562, and S180 cells, n=6; ( B ) tumor weight of S180 mice orally treated with 5 μmol/kg/day ICCA for 9 consecutive days, n=12; ( C ) Cr of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( D ) ALT of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( E ) AST of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( F ) BUN of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6. Abbreviations: 95D, nonsmall cell lung cancer cell line; ALT, alanine transaminase; AST, aspartate transaminase; BUN, blood urea nitrogen; CMCNa, carboxymethyl cellulose sodium; Cr, creatinine; DOX, doxorubicin; ICCA, 1-(4-isopropylphenyl)-β-carboline-3-carboxylic acid; K562, human myeloid leukemia; S180, ascites tumor cells; Kar, Karmen.
    Figure Legend Snippet: Antiproliferation activity in vitro, antitumor activity in vivo, and toxicity of ICCA: ( A ) cell viabilities of ICCA-treated A549, 95D, K562, and S180 cells, n=6; ( B ) tumor weight of S180 mice orally treated with 5 μmol/kg/day ICCA for 9 consecutive days, n=12; ( C ) Cr of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( D ) ALT of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( E ) AST of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( F ) BUN of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6. Abbreviations: 95D, nonsmall cell lung cancer cell line; ALT, alanine transaminase; AST, aspartate transaminase; BUN, blood urea nitrogen; CMCNa, carboxymethyl cellulose sodium; Cr, creatinine; DOX, doxorubicin; ICCA, 1-(4-isopropylphenyl)-β-carboline-3-carboxylic acid; K562, human myeloid leukemia; S180, ascites tumor cells; Kar, Karmen.

    Techniques Used: Activity Assay, In Vitro, In Vivo

    human nonsmall cell lung cancer cell lines  (ATCC)


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    ATCC human nonsmall cell lung cancer cell lines
    Human Nonsmall Cell Lung Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human nonsmall cell lung cancer cell lines  (ATCC)


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    ATCC human nonsmall cell lung cancer cell lines
    a List of tested drugs and their chemical structures. b The effect of an anti-endometriosis drug, danazol, on the viability of <t>nonsmall</t> cell lung cell lines. c The effect of danazol on the apoptosis induction. Cell lines were treated with various concentrations of danazol. The horizontal axis represents the concentration on a logarithmic scale. The vertical axis represents the relative viability and relative apoptosis induction (caspase activity). Plot shows means and standard deviations for triplicate experiments.
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    1) Product Images from "Regulome-based characterization of drug activity across the human diseasome"

    Article Title: Regulome-based characterization of drug activity across the human diseasome

    Journal: NPJ Systems Biology and Applications

    doi: 10.1038/s41540-022-00255-4

    a List of tested drugs and their chemical structures. b The effect of an anti-endometriosis drug, danazol, on the viability of nonsmall cell lung cell lines. c The effect of danazol on the apoptosis induction. Cell lines were treated with various concentrations of danazol. The horizontal axis represents the concentration on a logarithmic scale. The vertical axis represents the relative viability and relative apoptosis induction (caspase activity). Plot shows means and standard deviations for triplicate experiments.
    Figure Legend Snippet: a List of tested drugs and their chemical structures. b The effect of an anti-endometriosis drug, danazol, on the viability of nonsmall cell lung cell lines. c The effect of danazol on the apoptosis induction. Cell lines were treated with various concentrations of danazol. The horizontal axis represents the concentration on a logarithmic scale. The vertical axis represents the relative viability and relative apoptosis induction (caspase activity). Plot shows means and standard deviations for triplicate experiments.

    Techniques Used: Concentration Assay, Activity Assay

    pd l1 human nonsmall cell lung cancer cell line  (ATCC)


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    ATCC pd l1 human nonsmall cell lung cancer cell line
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    ATCC human nonsmall cell lung cancer cell line
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    ATCC human nonsmall cell lung cancer cell line a549
    Human Nonsmall Cell Lung Cancer Cell Line A549, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC a549 nonsmall cell lung cancer cells line
    (a) Immunocytochemical staining and (b) protein level of EGFR expression in LO2 cells, HeLa cells, and <t>A549</t> cells. Scale bar = 50 µ m. (c) FL images of A549 cells after 4 h of incubation with free ICG, ICG-LPs, GE11-ICG-LPs, and GE11-ICG-LPs with free GE11 peptide or anti-EGFR antibody blocking. DAPI (blue) counterstains cell nuclei. Scale bar = 50 µ m. (d) The semiquantitative analysis of fluorescence intensity in (c) was determined by ‘Image J' software. Data are expressed as mean ± SD ( n = 3). (e) FL images of A549 cells incubated with GE11-CUR/ICG-LPs after laser irradiation (1 W·cm −2 ) for different times. Scale bar = 50 µ m.
    A549 Nonsmall Cell Lung Cancer Cells Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human nonsmall cell lung cancer cell lines
    CMIP is upregulated in LUAD and associated with poor prognosis. (a) Immunohistochemical tissue microarray image (HPA) of CMIP protein. (b) qRT-PCR detection of CMIP mRNA levels in normal lung tissue and LUAD tissue ( n = 20); (c) Western blot detection of CMIP protein expression in normal lung tissue and LUAD tissue ( n = 3); (d) mRNA levels of CMIP detected by qRT-PCR in BEAS-2B, <t>A549,</t> <t>H460,</t> and <t>H1299</t> cells ( n = 3); (e) OS and PFS in LUAD patients obtained from the Kaplan–Meier plotter online database; ∗∗ P < 0.01 vs. (Normal group or BEAS-2B group).
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    ATCC human nonsmall cell lung cancer nsclc cell lines h1299
    Effect of sirtinol on cellular proliferation of <t>H1299</t> cells. H1299 cells treated with different concentrations (5, 10, 20 and 50 μ M) of sirtinol for 24 h and 48 h, respectively. The cell survival was determined by the trypan blue staining assay combined with the Countess Automated Cell Counter. ** P < 0.001 against vehicle control.
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    ATCC human nonsmall cell lung cancer cells
    Antiproliferation activity in vitro, antitumor activity in vivo, and toxicity of ICCA: ( A ) cell viabilities of ICCA-treated <t>A549,</t> 95D, K562, and S180 cells, n=6; ( B ) tumor weight of S180 mice orally treated with 5 μmol/kg/day ICCA for 9 consecutive days, n=12; ( C ) Cr of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( D ) ALT of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( E ) AST of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( F ) BUN of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6. Abbreviations: 95D, <t>nonsmall</t> cell lung cancer cell line; ALT, alanine transaminase; AST, aspartate transaminase; BUN, blood urea nitrogen; CMCNa, carboxymethyl cellulose sodium; Cr, creatinine; DOX, doxorubicin; ICCA, 1-(4-isopropylphenyl)-β-carboline-3-carboxylic acid; K562, human myeloid leukemia; S180, ascites tumor cells; Kar, Karmen.
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    ATCC pd l1 human nonsmall cell lung cancer cell line
    Antiproliferation activity in vitro, antitumor activity in vivo, and toxicity of ICCA: ( A ) cell viabilities of ICCA-treated <t>A549,</t> 95D, K562, and S180 cells, n=6; ( B ) tumor weight of S180 mice orally treated with 5 μmol/kg/day ICCA for 9 consecutive days, n=12; ( C ) Cr of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( D ) ALT of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( E ) AST of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( F ) BUN of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6. Abbreviations: 95D, <t>nonsmall</t> cell lung cancer cell line; ALT, alanine transaminase; AST, aspartate transaminase; BUN, blood urea nitrogen; CMCNa, carboxymethyl cellulose sodium; Cr, creatinine; DOX, doxorubicin; ICCA, 1-(4-isopropylphenyl)-β-carboline-3-carboxylic acid; K562, human myeloid leukemia; S180, ascites tumor cells; Kar, Karmen.
    Pd L1 Human Nonsmall Cell Lung Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (a) Immunocytochemical staining and (b) protein level of EGFR expression in LO2 cells, HeLa cells, and A549 cells. Scale bar = 50 µ m. (c) FL images of A549 cells after 4 h of incubation with free ICG, ICG-LPs, GE11-ICG-LPs, and GE11-ICG-LPs with free GE11 peptide or anti-EGFR antibody blocking. DAPI (blue) counterstains cell nuclei. Scale bar = 50 µ m. (d) The semiquantitative analysis of fluorescence intensity in (c) was determined by ‘Image J' software. Data are expressed as mean ± SD ( n = 3). (e) FL images of A549 cells incubated with GE11-CUR/ICG-LPs after laser irradiation (1 W·cm −2 ) for different times. Scale bar = 50 µ m.

    Journal: Bioinorganic Chemistry and Applications

    Article Title: GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

    doi: 10.1155/2021/5534870

    Figure Lengend Snippet: (a) Immunocytochemical staining and (b) protein level of EGFR expression in LO2 cells, HeLa cells, and A549 cells. Scale bar = 50 µ m. (c) FL images of A549 cells after 4 h of incubation with free ICG, ICG-LPs, GE11-ICG-LPs, and GE11-ICG-LPs with free GE11 peptide or anti-EGFR antibody blocking. DAPI (blue) counterstains cell nuclei. Scale bar = 50 µ m. (d) The semiquantitative analysis of fluorescence intensity in (c) was determined by ‘Image J' software. Data are expressed as mean ± SD ( n = 3). (e) FL images of A549 cells incubated with GE11-CUR/ICG-LPs after laser irradiation (1 W·cm −2 ) for different times. Scale bar = 50 µ m.

    Article Snippet: A549 nonsmall cell lung cancer cells line, HeLa human cervical cancer cell and LO2 human normal liver cells were purchased from American Type Culture Collection (ATCC, USA).

    Techniques: Staining, Expressing, Incubation, Blocking Assay, Fluorescence, Software, Irradiation

    (a), (b) Cytotoxicity and phototoxicity of free CUR/ICG, CUR/ICG-LPs and GE11-CUR/ICG-LPs under the NIR laser irradiation (808 nm) of 1 W·cm −2 for 5 min at different concentrations on A549 cells after 24 h incubation. (c) Fluorescence images of A549 cells stained with calcein-AM (green) after different treatments. Scale bar = 100 μ m. (d) Synergistic effect of photo- and chemotherapy based on GE11-CUR/ICG-LPs. Cytotoxicity of GE11-ICG-LPs, GE11-CUR-LPs, and GE11-CUR/ICG-LPs with or without laser exposure was performed by MTT assays. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

    Journal: Bioinorganic Chemistry and Applications

    Article Title: GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

    doi: 10.1155/2021/5534870

    Figure Lengend Snippet: (a), (b) Cytotoxicity and phototoxicity of free CUR/ICG, CUR/ICG-LPs and GE11-CUR/ICG-LPs under the NIR laser irradiation (808 nm) of 1 W·cm −2 for 5 min at different concentrations on A549 cells after 24 h incubation. (c) Fluorescence images of A549 cells stained with calcein-AM (green) after different treatments. Scale bar = 100 μ m. (d) Synergistic effect of photo- and chemotherapy based on GE11-CUR/ICG-LPs. Cytotoxicity of GE11-ICG-LPs, GE11-CUR-LPs, and GE11-CUR/ICG-LPs with or without laser exposure was performed by MTT assays. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

    Article Snippet: A549 nonsmall cell lung cancer cells line, HeLa human cervical cancer cell and LO2 human normal liver cells were purchased from American Type Culture Collection (ATCC, USA).

    Techniques: Irradiation, Incubation, Fluorescence, Staining

    (a) Effects of IC-GLPs on the caspase-3 activity in A549 cells, data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001. (b) Intracellular ROS detection by the DCFH-DA fluorescence staining. Scale bar = 50 μ m. (c) Cytoskeletal microtubulin (red) expression in A549 cells after different treatments with laser irradiation. DAPI (blue) counterstains cell nuclei. Scale bar = 10 μ m.

    Journal: Bioinorganic Chemistry and Applications

    Article Title: GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

    doi: 10.1155/2021/5534870

    Figure Lengend Snippet: (a) Effects of IC-GLPs on the caspase-3 activity in A549 cells, data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001. (b) Intracellular ROS detection by the DCFH-DA fluorescence staining. Scale bar = 50 μ m. (c) Cytoskeletal microtubulin (red) expression in A549 cells after different treatments with laser irradiation. DAPI (blue) counterstains cell nuclei. Scale bar = 10 μ m.

    Article Snippet: A549 nonsmall cell lung cancer cells line, HeLa human cervical cancer cell and LO2 human normal liver cells were purchased from American Type Culture Collection (ATCC, USA).

    Techniques: Activity Assay, Fluorescence, Staining, Expressing, Irradiation

    (a) Representative western blot and (b) quantification of Bcl-2 and Bax expression in A549 cells following treatment with PBS, free CUR/ICG, CUR/ICG-LPs, and GE11-CUR/ICG-LPs under 808 nm laser irradiation for 5 min. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05 and ∗∗ P < 0.01. (c) Representative western blot and (d) quantification of PI3K, p-PI3K, Akt, and p-Akt expression in A549 cells following different treatments with laser irradiation. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

    Journal: Bioinorganic Chemistry and Applications

    Article Title: GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

    doi: 10.1155/2021/5534870

    Figure Lengend Snippet: (a) Representative western blot and (b) quantification of Bcl-2 and Bax expression in A549 cells following treatment with PBS, free CUR/ICG, CUR/ICG-LPs, and GE11-CUR/ICG-LPs under 808 nm laser irradiation for 5 min. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05 and ∗∗ P < 0.01. (c) Representative western blot and (d) quantification of PI3K, p-PI3K, Akt, and p-Akt expression in A549 cells following different treatments with laser irradiation. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

    Article Snippet: A549 nonsmall cell lung cancer cells line, HeLa human cervical cancer cell and LO2 human normal liver cells were purchased from American Type Culture Collection (ATCC, USA).

    Techniques: Western Blot, Expressing, Irradiation

    CMIP is upregulated in LUAD and associated with poor prognosis. (a) Immunohistochemical tissue microarray image (HPA) of CMIP protein. (b) qRT-PCR detection of CMIP mRNA levels in normal lung tissue and LUAD tissue ( n = 20); (c) Western blot detection of CMIP protein expression in normal lung tissue and LUAD tissue ( n = 3); (d) mRNA levels of CMIP detected by qRT-PCR in BEAS-2B, A549, H460, and H1299 cells ( n = 3); (e) OS and PFS in LUAD patients obtained from the Kaplan–Meier plotter online database; ∗∗ P < 0.01 vs. (Normal group or BEAS-2B group).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: CMaf-Inducing Protein Promotes LUAD Proliferation and Metastasis by Activating the MAPK/ERK Pathway

    doi: 10.1155/2022/2501846

    Figure Lengend Snippet: CMIP is upregulated in LUAD and associated with poor prognosis. (a) Immunohistochemical tissue microarray image (HPA) of CMIP protein. (b) qRT-PCR detection of CMIP mRNA levels in normal lung tissue and LUAD tissue ( n = 20); (c) Western blot detection of CMIP protein expression in normal lung tissue and LUAD tissue ( n = 3); (d) mRNA levels of CMIP detected by qRT-PCR in BEAS-2B, A549, H460, and H1299 cells ( n = 3); (e) OS and PFS in LUAD patients obtained from the Kaplan–Meier plotter online database; ∗∗ P < 0.01 vs. (Normal group or BEAS-2B group).

    Article Snippet: Cell culture human normal lung epithelial cells (BEAS-2B) and human nonsmall cell lung cancer cell lines (A549, H460 and H1299) were purchased from American type culture collection (ATCC).

    Techniques: Immunohistochemical staining, Microarray, Quantitative RT-PCR, Western Blot, Expressing

    CMIP overexpression promotes the proliferation of H460 cells. (a/b) The mRNA levels of CMIP in H460 (a) and H1299 (b) cells after transfection were detected by qRT-PCR; (c/d) MTT assay was used to detect the difference of CMIP expression after transfection on H460 (c) and H1299 (d) Effect of cell viability. (e/f) Cell colony formation assay to assess the effect of CMIP expression on the proliferation of H460 (e) and H1299 (f) cells after transfection. ∗∗ P < 0.01 vs. (siNC group or vector group).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: CMaf-Inducing Protein Promotes LUAD Proliferation and Metastasis by Activating the MAPK/ERK Pathway

    doi: 10.1155/2022/2501846

    Figure Lengend Snippet: CMIP overexpression promotes the proliferation of H460 cells. (a/b) The mRNA levels of CMIP in H460 (a) and H1299 (b) cells after transfection were detected by qRT-PCR; (c/d) MTT assay was used to detect the difference of CMIP expression after transfection on H460 (c) and H1299 (d) Effect of cell viability. (e/f) Cell colony formation assay to assess the effect of CMIP expression on the proliferation of H460 (e) and H1299 (f) cells after transfection. ∗∗ P < 0.01 vs. (siNC group or vector group).

    Article Snippet: Cell culture human normal lung epithelial cells (BEAS-2B) and human nonsmall cell lung cancer cell lines (A549, H460 and H1299) were purchased from American type culture collection (ATCC).

    Techniques: Over Expression, Transfection, Quantitative RT-PCR, MTT Assay, Expressing, Colony Assay, Plasmid Preparation

    CMIP overexpression promotes H460 cell migration and invasion. (a/b) The wound healing assay was used to analyze the effect of CMIP expression on the migration of H460 (a) and H1299 (b) cells after transfection. (c/d) The effect of CMIP expression on H460 (c) and H1299 (d) cell invasion after transfection was assessed using Transwell analysis. ∗∗ P < 0.01 vs. (siNC group or vector group).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: CMaf-Inducing Protein Promotes LUAD Proliferation and Metastasis by Activating the MAPK/ERK Pathway

    doi: 10.1155/2022/2501846

    Figure Lengend Snippet: CMIP overexpression promotes H460 cell migration and invasion. (a/b) The wound healing assay was used to analyze the effect of CMIP expression on the migration of H460 (a) and H1299 (b) cells after transfection. (c/d) The effect of CMIP expression on H460 (c) and H1299 (d) cell invasion after transfection was assessed using Transwell analysis. ∗∗ P < 0.01 vs. (siNC group or vector group).

    Article Snippet: Cell culture human normal lung epithelial cells (BEAS-2B) and human nonsmall cell lung cancer cell lines (A549, H460 and H1299) were purchased from American type culture collection (ATCC).

    Techniques: Over Expression, Migration, Wound Healing Assay, Expressing, Transfection, Plasmid Preparation

    CMIP overexpression promotes activation of the MAPK/ERK pathway. (a, b) Western blot detection of P38, ERK, p-P38 and p-ERK protein expression, and the relative expression of p-P38/P38 and p-ERK/ERK in H1299 cells. (c, d) Western blot detection of P38, ERK, p-P38 and p-ERK protein expression, and the relative expression of p-P38/P38 and p-ERK/ERK in H460 cells. ∗∗ P < 0.01 vs. (siNC group or vector group).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: CMaf-Inducing Protein Promotes LUAD Proliferation and Metastasis by Activating the MAPK/ERK Pathway

    doi: 10.1155/2022/2501846

    Figure Lengend Snippet: CMIP overexpression promotes activation of the MAPK/ERK pathway. (a, b) Western blot detection of P38, ERK, p-P38 and p-ERK protein expression, and the relative expression of p-P38/P38 and p-ERK/ERK in H1299 cells. (c, d) Western blot detection of P38, ERK, p-P38 and p-ERK protein expression, and the relative expression of p-P38/P38 and p-ERK/ERK in H460 cells. ∗∗ P < 0.01 vs. (siNC group or vector group).

    Article Snippet: Cell culture human normal lung epithelial cells (BEAS-2B) and human nonsmall cell lung cancer cell lines (A549, H460 and H1299) were purchased from American type culture collection (ATCC).

    Techniques: Over Expression, Activation Assay, Western Blot, Expressing, Plasmid Preparation

    CMIP promotes H460 cell proliferation, migration, and invasion by activating the MAPK/ERK pathway. (a) MTT assay to detect cell viability in each group; (b) cell clone formation assay to detect the number of cell clones in each group; (c) wound healing assay to detect cell migration in each group; (d) Transwell analysis to detect cell invasion ability of each group. ∗ P < 0.05 and ∗∗ P < 0.01 vs. vector group, ## P < 0.01 vs. OE-CMIP group.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: CMaf-Inducing Protein Promotes LUAD Proliferation and Metastasis by Activating the MAPK/ERK Pathway

    doi: 10.1155/2022/2501846

    Figure Lengend Snippet: CMIP promotes H460 cell proliferation, migration, and invasion by activating the MAPK/ERK pathway. (a) MTT assay to detect cell viability in each group; (b) cell clone formation assay to detect the number of cell clones in each group; (c) wound healing assay to detect cell migration in each group; (d) Transwell analysis to detect cell invasion ability of each group. ∗ P < 0.05 and ∗∗ P < 0.01 vs. vector group, ## P < 0.01 vs. OE-CMIP group.

    Article Snippet: Cell culture human normal lung epithelial cells (BEAS-2B) and human nonsmall cell lung cancer cell lines (A549, H460 and H1299) were purchased from American type culture collection (ATCC).

    Techniques: Migration, MTT Assay, Tube Formation Assay, Clone Assay, Wound Healing Assay, Plasmid Preparation

    Effect of sirtinol on cellular proliferation of H1299 cells. H1299 cells treated with different concentrations (5, 10, 20 and 50 μ M) of sirtinol for 24 h and 48 h, respectively. The cell survival was determined by the trypan blue staining assay combined with the Countess Automated Cell Counter. ** P < 0.001 against vehicle control.

    Journal: The Scientific World Journal

    Article Title: The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis

    doi: 10.1155/2014/937051

    Figure Lengend Snippet: Effect of sirtinol on cellular proliferation of H1299 cells. H1299 cells treated with different concentrations (5, 10, 20 and 50 μ M) of sirtinol for 24 h and 48 h, respectively. The cell survival was determined by the trypan blue staining assay combined with the Countess Automated Cell Counter. ** P < 0.001 against vehicle control.

    Article Snippet: Human nonsmall cell lung cancer (NSCLC) cell lines H1299 were obtained from American Type Culture Collection (ATCC; Virginia, USA).

    Techniques: Staining

    Sirtinol inhibits the colony formation of lung cancer cells. H1299 cells were treated with different concentrations (5, 10, 20 and 50 μ M) of sirtinol for 15 days, respectively. Afterwards, the cells were glutaraldehyde-fixed and stained with Giemsa stain for 1 h. (a) The colony formation analysis of H1299 cells. (b) The quantification analysis of the colony diameter. Data are represented as mean ± SD ( n = 3). * P < 0.01 compared with the vehicle control.

    Journal: The Scientific World Journal

    Article Title: The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis

    doi: 10.1155/2014/937051

    Figure Lengend Snippet: Sirtinol inhibits the colony formation of lung cancer cells. H1299 cells were treated with different concentrations (5, 10, 20 and 50 μ M) of sirtinol for 15 days, respectively. Afterwards, the cells were glutaraldehyde-fixed and stained with Giemsa stain for 1 h. (a) The colony formation analysis of H1299 cells. (b) The quantification analysis of the colony diameter. Data are represented as mean ± SD ( n = 3). * P < 0.01 compared with the vehicle control.

    Article Snippet: Human nonsmall cell lung cancer (NSCLC) cell lines H1299 were obtained from American Type Culture Collection (ATCC; Virginia, USA).

    Techniques: Staining, Giemsa Stain

    Sirtinol induces apoptosis of H1299 cells. Cells were treated with indicated concentrations of sirtinol and stained with Annexin-V and PI at 24 h, respectively. (a) Flow cytometry profiling represents the results of Annexin-V-FITC staining. (b) The quantificative analysis of cell apoptosis. Different letter notations indicate the statistical significance between sirtinol treatment and vehicle ( a versus b and a versus c indicate the P < 0.05 and 0.001, resp.).

    Journal: The Scientific World Journal

    Article Title: The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis

    doi: 10.1155/2014/937051

    Figure Lengend Snippet: Sirtinol induces apoptosis of H1299 cells. Cells were treated with indicated concentrations of sirtinol and stained with Annexin-V and PI at 24 h, respectively. (a) Flow cytometry profiling represents the results of Annexin-V-FITC staining. (b) The quantificative analysis of cell apoptosis. Different letter notations indicate the statistical significance between sirtinol treatment and vehicle ( a versus b and a versus c indicate the P < 0.05 and 0.001, resp.).

    Article Snippet: Human nonsmall cell lung cancer (NSCLC) cell lines H1299 were obtained from American Type Culture Collection (ATCC; Virginia, USA).

    Techniques: Staining, Flow Cytometry

    The effect of sirtinol on cell cycle distribution of lung cancer cells. H1299 cells treated with indicated concentrations (from 5 to 50 μ M) of sirtinol for 24 h, respectively. Cells were stained with PI and detected the cell cycle distribution by flow cytometry. (a) Flow cytometry profile represents PI staining in x -axis and cell number in y -axis. (b) The quantitative analysis of cell cycle distribution. Different letter notations indicate the statistical significance between drug treatment and vehicle ( a versus b and a versus c indicate the P < 0.05 and 0.001, resp.).

    Journal: The Scientific World Journal

    Article Title: The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis

    doi: 10.1155/2014/937051

    Figure Lengend Snippet: The effect of sirtinol on cell cycle distribution of lung cancer cells. H1299 cells treated with indicated concentrations (from 5 to 50 μ M) of sirtinol for 24 h, respectively. Cells were stained with PI and detected the cell cycle distribution by flow cytometry. (a) Flow cytometry profile represents PI staining in x -axis and cell number in y -axis. (b) The quantitative analysis of cell cycle distribution. Different letter notations indicate the statistical significance between drug treatment and vehicle ( a versus b and a versus c indicate the P < 0.05 and 0.001, resp.).

    Article Snippet: Human nonsmall cell lung cancer (NSCLC) cell lines H1299 were obtained from American Type Culture Collection (ATCC; Virginia, USA).

    Techniques: Staining, Flow Cytometry

    Modulation of protein levels in NSCLC H1299 cells after sirtinol treatment. H1299 cells treated with indicated concentrations (10, 20, and 50 μ M) of sirtinol for 24 h, respectively. The results of Western blot of Sirt1 nonhistone target protein, including FoxO3a, Akt phosphorylation, and β -catenin. β -Actin as an internal control.

    Journal: The Scientific World Journal

    Article Title: The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis

    doi: 10.1155/2014/937051

    Figure Lengend Snippet: Modulation of protein levels in NSCLC H1299 cells after sirtinol treatment. H1299 cells treated with indicated concentrations (10, 20, and 50 μ M) of sirtinol for 24 h, respectively. The results of Western blot of Sirt1 nonhistone target protein, including FoxO3a, Akt phosphorylation, and β -catenin. β -Actin as an internal control.

    Article Snippet: Human nonsmall cell lung cancer (NSCLC) cell lines H1299 were obtained from American Type Culture Collection (ATCC; Virginia, USA).

    Techniques: Western Blot

    Possible model of sirtinol-induced antiproliferation and apoptosis in lung cancer cells. Sirtinol downregulates the activation of prosurvival Akt serine/threonine kinase and the protein level of β -catenin, a proliferation-associated transcription factor, insulting in the cell cycle G 1 -phase accumulation and the growth arrest. On the contrary, sirtinol treatment causes the upregulation of the proapoptotic transcription factor FoxO3a, a target of both Akt signaling and Sirt1. This may render H1299 cells more sensitive to apoptosis. Finally, sirtinol induces the apoptosis of lung cancer cells.

    Journal: The Scientific World Journal

    Article Title: The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis

    doi: 10.1155/2014/937051

    Figure Lengend Snippet: Possible model of sirtinol-induced antiproliferation and apoptosis in lung cancer cells. Sirtinol downregulates the activation of prosurvival Akt serine/threonine kinase and the protein level of β -catenin, a proliferation-associated transcription factor, insulting in the cell cycle G 1 -phase accumulation and the growth arrest. On the contrary, sirtinol treatment causes the upregulation of the proapoptotic transcription factor FoxO3a, a target of both Akt signaling and Sirt1. This may render H1299 cells more sensitive to apoptosis. Finally, sirtinol induces the apoptosis of lung cancer cells.

    Article Snippet: Human nonsmall cell lung cancer (NSCLC) cell lines H1299 were obtained from American Type Culture Collection (ATCC; Virginia, USA).

    Techniques: Activation Assay

    Antiproliferation activity in vitro, antitumor activity in vivo, and toxicity of ICCA: ( A ) cell viabilities of ICCA-treated A549, 95D, K562, and S180 cells, n=6; ( B ) tumor weight of S180 mice orally treated with 5 μmol/kg/day ICCA for 9 consecutive days, n=12; ( C ) Cr of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( D ) ALT of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( E ) AST of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( F ) BUN of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6. Abbreviations: 95D, nonsmall cell lung cancer cell line; ALT, alanine transaminase; AST, aspartate transaminase; BUN, blood urea nitrogen; CMCNa, carboxymethyl cellulose sodium; Cr, creatinine; DOX, doxorubicin; ICCA, 1-(4-isopropylphenyl)-β-carboline-3-carboxylic acid; K562, human myeloid leukemia; S180, ascites tumor cells; Kar, Karmen.

    Journal: Drug Design, Development and Therapy

    Article Title: Design and development of ICCA as a dual inhibitor of GPIIb/IIIa and P-selectin receptors

    doi: 10.2147/DDDT.S169238

    Figure Lengend Snippet: Antiproliferation activity in vitro, antitumor activity in vivo, and toxicity of ICCA: ( A ) cell viabilities of ICCA-treated A549, 95D, K562, and S180 cells, n=6; ( B ) tumor weight of S180 mice orally treated with 5 μmol/kg/day ICCA for 9 consecutive days, n=12; ( C ) Cr of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( D ) ALT of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( E ) AST of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( F ) BUN of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6. Abbreviations: 95D, nonsmall cell lung cancer cell line; ALT, alanine transaminase; AST, aspartate transaminase; BUN, blood urea nitrogen; CMCNa, carboxymethyl cellulose sodium; Cr, creatinine; DOX, doxorubicin; ICCA, 1-(4-isopropylphenyl)-β-carboline-3-carboxylic acid; K562, human myeloid leukemia; S180, ascites tumor cells; Kar, Karmen.

    Article Snippet: Human myeloid leukemia cells (K562), human nonsmall cell lung cancer cells (A549), and nonsmall cell lung cancer cells (95D) were purchased from American Type Culture Collection (Manassas, VA, USA).

    Techniques: Activity Assay, In Vitro, In Vivo