human nonsmall cell lung cancer cell line a549  (ATCC)

Journal: Bioinorganic Chemistry and Applications

Article Title: GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

doi: 10.1155/2021/5534870

Figure Lengend Snippet: (a) Immunocytochemical staining and (b) protein level of EGFR expression in LO2 cells, HeLa cells, and A549 cells. Scale bar = 50 µ m. (c) FL images of A549 cells after 4 h of incubation with free ICG, ICG-LPs, GE11-ICG-LPs, and GE11-ICG-LPs with free GE11 peptide or anti-EGFR antibody blocking. DAPI (blue) counterstains cell nuclei. Scale bar = 50 µ m. (d) The semiquantitative analysis of fluorescence intensity in (c) was determined by ‘Image J' software. Data are expressed as mean ± SD ( n = 3). (e) FL images of A549 cells incubated with GE11-CUR/ICG-LPs after laser irradiation (1 W·cm −2 ) for different times. Scale bar = 50 µ m.

Article Snippet: A549 nonsmall cell lung cancer cells line, HeLa human cervical cancer cell and LO2 human normal liver cells were purchased from American Type Culture Collection (ATCC, USA).

Techniques: Staining, Expressing, Incubation, Blocking Assay, Fluorescence, Software, Irradiation

Journal: Bioinorganic Chemistry and Applications

Article Title: GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

doi: 10.1155/2021/5534870

Figure Lengend Snippet: (a), (b) Cytotoxicity and phototoxicity of free CUR/ICG, CUR/ICG-LPs and GE11-CUR/ICG-LPs under the NIR laser irradiation (808 nm) of 1 W·cm −2 for 5 min at different concentrations on A549 cells after 24 h incubation. (c) Fluorescence images of A549 cells stained with calcein-AM (green) after different treatments. Scale bar = 100 μ m. (d) Synergistic effect of photo- and chemotherapy based on GE11-CUR/ICG-LPs. Cytotoxicity of GE11-ICG-LPs, GE11-CUR-LPs, and GE11-CUR/ICG-LPs with or without laser exposure was performed by MTT assays. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

Article Snippet: A549 nonsmall cell lung cancer cells line, HeLa human cervical cancer cell and LO2 human normal liver cells were purchased from American Type Culture Collection (ATCC, USA).

Techniques: Irradiation, Incubation, Fluorescence, Staining

Journal: Bioinorganic Chemistry and Applications

Article Title: GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

doi: 10.1155/2021/5534870

Figure Lengend Snippet: (a) Effects of IC-GLPs on the caspase-3 activity in A549 cells, data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001. (b) Intracellular ROS detection by the DCFH-DA fluorescence staining. Scale bar = 50 μ m. (c) Cytoskeletal microtubulin (red) expression in A549 cells after different treatments with laser irradiation. DAPI (blue) counterstains cell nuclei. Scale bar = 10 μ m.

Article Snippet: A549 nonsmall cell lung cancer cells line, HeLa human cervical cancer cell and LO2 human normal liver cells were purchased from American Type Culture Collection (ATCC, USA).

Techniques: Activity Assay, Fluorescence, Staining, Expressing, Irradiation

Journal: Bioinorganic Chemistry and Applications

Article Title: GE11 Peptide Conjugated Liposomes for EGFR-Targeted and Chemophotothermal Combined Anticancer Therapy

doi: 10.1155/2021/5534870

Figure Lengend Snippet: (a) Representative western blot and (b) quantification of Bcl-2 and Bax expression in A549 cells following treatment with PBS, free CUR/ICG, CUR/ICG-LPs, and GE11-CUR/ICG-LPs under 808 nm laser irradiation for 5 min. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05 and ∗∗ P < 0.01. (c) Representative western blot and (d) quantification of PI3K, p-PI3K, Akt, and p-Akt expression in A549 cells following different treatments with laser irradiation. Data are expressed as mean ± SD ( n = 3). ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

Article Snippet: A549 nonsmall cell lung cancer cells line, HeLa human cervical cancer cell and LO2 human normal liver cells were purchased from American Type Culture Collection (ATCC, USA).

Techniques: Western Blot, Expressing, Irradiation

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: CMaf-Inducing Protein Promotes LUAD Proliferation and Metastasis by Activating the MAPK/ERK Pathway

doi: 10.1155/2022/2501846

Figure Lengend Snippet: CMIP is upregulated in LUAD and associated with poor prognosis. (a) Immunohistochemical tissue microarray image (HPA) of CMIP protein. (b) qRT-PCR detection of CMIP mRNA levels in normal lung tissue and LUAD tissue ( n = 20); (c) Western blot detection of CMIP protein expression in normal lung tissue and LUAD tissue ( n = 3); (d) mRNA levels of CMIP detected by qRT-PCR in BEAS-2B, A549, H460, and H1299 cells ( n = 3); (e) OS and PFS in LUAD patients obtained from the Kaplan–Meier plotter online database; ∗∗ P < 0.01 vs. (Normal group or BEAS-2B group).

Article Snippet: Cell culture human normal lung epithelial cells (BEAS-2B) and human nonsmall cell lung cancer cell lines (A549, H460 and H1299) were purchased from American type culture collection (ATCC).

Techniques: Immunohistochemical staining, Microarray, Quantitative RT-PCR, Western Blot, Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: CMaf-Inducing Protein Promotes LUAD Proliferation and Metastasis by Activating the MAPK/ERK Pathway

doi: 10.1155/2022/2501846

Figure Lengend Snippet: CMIP overexpression promotes the proliferation of H460 cells. (a/b) The mRNA levels of CMIP in H460 (a) and H1299 (b) cells after transfection were detected by qRT-PCR; (c/d) MTT assay was used to detect the difference of CMIP expression after transfection on H460 (c) and H1299 (d) Effect of cell viability. (e/f) Cell colony formation assay to assess the effect of CMIP expression on the proliferation of H460 (e) and H1299 (f) cells after transfection. ∗∗ P < 0.01 vs. (siNC group or vector group).

Article Snippet: Cell culture human normal lung epithelial cells (BEAS-2B) and human nonsmall cell lung cancer cell lines (A549, H460 and H1299) were purchased from American type culture collection (ATCC).

Techniques: Over Expression, Transfection, Quantitative RT-PCR, MTT Assay, Expressing, Colony Assay, Plasmid Preparation

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: CMaf-Inducing Protein Promotes LUAD Proliferation and Metastasis by Activating the MAPK/ERK Pathway

doi: 10.1155/2022/2501846

Figure Lengend Snippet: CMIP overexpression promotes H460 cell migration and invasion. (a/b) The wound healing assay was used to analyze the effect of CMIP expression on the migration of H460 (a) and H1299 (b) cells after transfection. (c/d) The effect of CMIP expression on H460 (c) and H1299 (d) cell invasion after transfection was assessed using Transwell analysis. ∗∗ P < 0.01 vs. (siNC group or vector group).

Article Snippet: Cell culture human normal lung epithelial cells (BEAS-2B) and human nonsmall cell lung cancer cell lines (A549, H460 and H1299) were purchased from American type culture collection (ATCC).

Techniques: Over Expression, Migration, Wound Healing Assay, Expressing, Transfection, Plasmid Preparation

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: CMaf-Inducing Protein Promotes LUAD Proliferation and Metastasis by Activating the MAPK/ERK Pathway

doi: 10.1155/2022/2501846

Figure Lengend Snippet: CMIP overexpression promotes activation of the MAPK/ERK pathway. (a, b) Western blot detection of P38, ERK, p-P38 and p-ERK protein expression, and the relative expression of p-P38/P38 and p-ERK/ERK in H1299 cells. (c, d) Western blot detection of P38, ERK, p-P38 and p-ERK protein expression, and the relative expression of p-P38/P38 and p-ERK/ERK in H460 cells. ∗∗ P < 0.01 vs. (siNC group or vector group).

Article Snippet: Cell culture human normal lung epithelial cells (BEAS-2B) and human nonsmall cell lung cancer cell lines (A549, H460 and H1299) were purchased from American type culture collection (ATCC).

Techniques: Over Expression, Activation Assay, Western Blot, Expressing, Plasmid Preparation

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: CMaf-Inducing Protein Promotes LUAD Proliferation and Metastasis by Activating the MAPK/ERK Pathway

doi: 10.1155/2022/2501846

Figure Lengend Snippet: CMIP promotes H460 cell proliferation, migration, and invasion by activating the MAPK/ERK pathway. (a) MTT assay to detect cell viability in each group; (b) cell clone formation assay to detect the number of cell clones in each group; (c) wound healing assay to detect cell migration in each group; (d) Transwell analysis to detect cell invasion ability of each group. ∗ P < 0.05 and ∗∗ P < 0.01 vs. vector group, ## P < 0.01 vs. OE-CMIP group.

Article Snippet: Cell culture human normal lung epithelial cells (BEAS-2B) and human nonsmall cell lung cancer cell lines (A549, H460 and H1299) were purchased from American type culture collection (ATCC).

Techniques: Migration, MTT Assay, Tube Formation Assay, Clone Assay, Wound Healing Assay, Plasmid Preparation

Journal: The Scientific World Journal

Article Title: The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis

doi: 10.1155/2014/937051

Figure Lengend Snippet: Effect of sirtinol on cellular proliferation of H1299 cells. H1299 cells treated with different concentrations (5, 10, 20 and 50 μ M) of sirtinol for 24 h and 48 h, respectively. The cell survival was determined by the trypan blue staining assay combined with the Countess Automated Cell Counter. ** P < 0.001 against vehicle control.

Article Snippet: Human nonsmall cell lung cancer (NSCLC) cell lines H1299 were obtained from American Type Culture Collection (ATCC; Virginia, USA).

Techniques: Staining

Journal: The Scientific World Journal

Article Title: The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis

doi: 10.1155/2014/937051

Figure Lengend Snippet: Sirtinol inhibits the colony formation of lung cancer cells. H1299 cells were treated with different concentrations (5, 10, 20 and 50 μ M) of sirtinol for 15 days, respectively. Afterwards, the cells were glutaraldehyde-fixed and stained with Giemsa stain for 1 h. (a) The colony formation analysis of H1299 cells. (b) The quantification analysis of the colony diameter. Data are represented as mean ± SD ( n = 3). * P < 0.01 compared with the vehicle control.

Article Snippet: Human nonsmall cell lung cancer (NSCLC) cell lines H1299 were obtained from American Type Culture Collection (ATCC; Virginia, USA).

Techniques: Staining, Giemsa Stain

Journal: The Scientific World Journal

Article Title: The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis

doi: 10.1155/2014/937051

Figure Lengend Snippet: Sirtinol induces apoptosis of H1299 cells. Cells were treated with indicated concentrations of sirtinol and stained with Annexin-V and PI at 24 h, respectively. (a) Flow cytometry profiling represents the results of Annexin-V-FITC staining. (b) The quantificative analysis of cell apoptosis. Different letter notations indicate the statistical significance between sirtinol treatment and vehicle ( a versus b and a versus c indicate the P < 0.05 and 0.001, resp.).

Article Snippet: Human nonsmall cell lung cancer (NSCLC) cell lines H1299 were obtained from American Type Culture Collection (ATCC; Virginia, USA).

Techniques: Staining, Flow Cytometry

Journal: The Scientific World Journal

Article Title: The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis

doi: 10.1155/2014/937051

Figure Lengend Snippet: The effect of sirtinol on cell cycle distribution of lung cancer cells. H1299 cells treated with indicated concentrations (from 5 to 50 μ M) of sirtinol for 24 h, respectively. Cells were stained with PI and detected the cell cycle distribution by flow cytometry. (a) Flow cytometry profile represents PI staining in x -axis and cell number in y -axis. (b) The quantitative analysis of cell cycle distribution. Different letter notations indicate the statistical significance between drug treatment and vehicle ( a versus b and a versus c indicate the P < 0.05 and 0.001, resp.).

Article Snippet: Human nonsmall cell lung cancer (NSCLC) cell lines H1299 were obtained from American Type Culture Collection (ATCC; Virginia, USA).

Techniques: Staining, Flow Cytometry

Journal: The Scientific World Journal

Article Title: The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis

doi: 10.1155/2014/937051

Figure Lengend Snippet: Modulation of protein levels in NSCLC H1299 cells after sirtinol treatment. H1299 cells treated with indicated concentrations (10, 20, and 50 μ M) of sirtinol for 24 h, respectively. The results of Western blot of Sirt1 nonhistone target protein, including FoxO3a, Akt phosphorylation, and β -catenin. β -Actin as an internal control.

Article Snippet: Human nonsmall cell lung cancer (NSCLC) cell lines H1299 were obtained from American Type Culture Collection (ATCC; Virginia, USA).

Techniques: Western Blot

Journal: The Scientific World Journal

Article Title: The Antiproliferative and Apoptotic Effects of Sirtinol, a Sirtuin Inhibitor on Human Lung Cancer Cells by Modulating Akt/ β -Catenin-Foxo3A Axis

doi: 10.1155/2014/937051

Figure Lengend Snippet: Possible model of sirtinol-induced antiproliferation and apoptosis in lung cancer cells. Sirtinol downregulates the activation of prosurvival Akt serine/threonine kinase and the protein level of β -catenin, a proliferation-associated transcription factor, insulting in the cell cycle G 1 -phase accumulation and the growth arrest. On the contrary, sirtinol treatment causes the upregulation of the proapoptotic transcription factor FoxO3a, a target of both Akt signaling and Sirt1. This may render H1299 cells more sensitive to apoptosis. Finally, sirtinol induces the apoptosis of lung cancer cells.

Article Snippet: Human nonsmall cell lung cancer (NSCLC) cell lines H1299 were obtained from American Type Culture Collection (ATCC; Virginia, USA).

Techniques: Activation Assay

Journal: Drug Design, Development and Therapy

Article Title: Design and development of ICCA as a dual inhibitor of GPIIb/IIIa and P-selectin receptors

doi: 10.2147/DDDT.S169238

Figure Lengend Snippet: Antiproliferation activity in vitro, antitumor activity in vivo, and toxicity of ICCA: ( A ) cell viabilities of ICCA-treated A549, 95D, K562, and S180 cells, n=6; ( B ) tumor weight of S180 mice orally treated with 5 μmol/kg/day ICCA for 9 consecutive days, n=12; ( C ) Cr of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( D ) ALT of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( E ) AST of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6; ( F ) BUN of S180 mice orally receiving 5 μmol/kg/day ICCA for 9 consecutive days, n=6. Abbreviations: 95D, nonsmall cell lung cancer cell line; ALT, alanine transaminase; AST, aspartate transaminase; BUN, blood urea nitrogen; CMCNa, carboxymethyl cellulose sodium; Cr, creatinine; DOX, doxorubicin; ICCA, 1-(4-isopropylphenyl)-β-carboline-3-carboxylic acid; K562, human myeloid leukemia; S180, ascites tumor cells; Kar, Karmen.

Article Snippet: Human myeloid leukemia cells (K562), human nonsmall cell lung cancer cells (A549), and nonsmall cell lung cancer cells (95D) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Activity Assay, In Vitro, In Vivo