human nanog  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    NANOG human
    Description:
    NANOG gene is localized to human chromosome 12p13 31 and has multiple pseudogenes This gene is composed of four exons three introns and a 915bp open reading frame ORF The encoded protein was first discovered in ESCs embryonic stem cells and is a divergent homeobox domain protein NANOG protein is composed of 305 amino acids and a central homeodomain HD This HD is composed of an unstructured N terminal arm a bundle of three α helices H1 H3 interlinked by loops Recombinant human Nanog is a 34 7kDa protein which is synthesized as a 304 amino acid polypeptide lacking a signal sequence for secretion
    Catalog Number:
    srp3119
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore human nanog
    NANOG gene is localized to human chromosome 12p13 31 and has multiple pseudogenes This gene is composed of four exons three introns and a 915bp open reading frame ORF The encoded protein was first discovered in ESCs embryonic stem cells and is a divergent homeobox domain protein NANOG protein is composed of 305 amino acids and a central homeodomain HD This HD is composed of an unstructured N terminal arm a bundle of three α helices H1 H3 interlinked by loops Recombinant human Nanog is a 34 7kDa protein which is synthesized as a 304 amino acid polypeptide lacking a signal sequence for secretion
    https://www.bioz.com/result/human nanog/product/Millipore
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human nanog - by Bioz Stars, 2020-07
    93/100 stars

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Radial shockwave treatment promotes human mesenchymal stem cell self-renewal and enhances cartilage healing
    Article Snippet: .. Human Nanog , Oct-4 , Sox-2 , Runx-2 , Osterix , CEBP/α , PPARγ , Sox-9 , and collagen type II ( Col-II ) cDNA were amplified by real-time PCR using a SYBR PCR Master Mix Kit (Sigma-Aldrich). ..

    Article Title: Radial shockwave treatment promotes human mesenchymal stem cell self-renewal and enhances cartilage healing
    Article Snippet: .. Human Nanog , Oct-4 , Sox-2 , Runx-2 , Osterix , CEBP/α , PPARγ , Sox-9 , and collagen type II (Col-II ) cDNA were amplified by real-time PCR using a SYBR PCR Master Mix Kit (Sigma-Aldrich). ..

    Polymerase Chain Reaction:

    Article Title: Radial shockwave treatment promotes human mesenchymal stem cell self-renewal and enhances cartilage healing
    Article Snippet: .. Human Nanog , Oct-4 , Sox-2 , Runx-2 , Osterix , CEBP/α , PPARγ , Sox-9 , and collagen type II ( Col-II ) cDNA were amplified by real-time PCR using a SYBR PCR Master Mix Kit (Sigma-Aldrich). ..

    Article Title: Radial shockwave treatment promotes human mesenchymal stem cell self-renewal and enhances cartilage healing
    Article Snippet: .. Human Nanog , Oct-4 , Sox-2 , Runx-2 , Osterix , CEBP/α , PPARγ , Sox-9 , and collagen type II (Col-II ) cDNA were amplified by real-time PCR using a SYBR PCR Master Mix Kit (Sigma-Aldrich). ..

    Incubation:

    Article Title: V-Myc Immortalizes Human Neural Stem Cells in the Absence of Pluripotency-Associated Traits
    Article Snippet: .. Cultures were blocked for 1h at room temperature in 10% normal horse serum or goat serum, 0.25% Triton X-100 in PBS (absent for surface proteins) and incubated overnight at 4°C with primary antibodies (dissolved in 1% serum and 0.25% Triton X-100 in PBS) against SSEA-1 (1:100, mouse mAb, Hybridoma Bank MC480), SSEA-4 (1:100, mouse mAb, Millipore MAB 34304), Tra 1–60 (1:100, mouse mAb, Millipore MAB 4360), and Tra 1–81 (1:100, mouse mAb, Millipore MAB4381, Nanog (1:100, goat pAb, R & D Systems AF1997), Oct3/4 (1:100, rabbit pAb, Santa Cruz Biotechnology SC9081), Sox2 (1:100, rabbit mAb, Millipore AB5603), and Alkaline Phosphatase (AP) (1:50, mouse mAb, R & D Systems MAB1448). .. After removal of the primary antibodies, cultures were incubated with the secondary antibodies as FICT-conjugated antibody (Alexa 488, 1:500, goat anti rabbit IgG, Molecular Probes A11034), Cy3-conjugated antibodies (Texas Red, 1:500, goat anti mouse IgM, Vector Laboratories TI-2020; Alexa 594, 1:500, chicken anti goat IgG, Molecular Probes a 21468; Cy3, 1:500, goat anti mouse IgG, Jackson Inmuno Research Lab., Inc. 111-165-003).

    Amplification:

    Article Title: Radial shockwave treatment promotes human mesenchymal stem cell self-renewal and enhances cartilage healing
    Article Snippet: .. Human Nanog , Oct-4 , Sox-2 , Runx-2 , Osterix , CEBP/α , PPARγ , Sox-9 , and collagen type II ( Col-II ) cDNA were amplified by real-time PCR using a SYBR PCR Master Mix Kit (Sigma-Aldrich). ..

    Article Title: Radial shockwave treatment promotes human mesenchymal stem cell self-renewal and enhances cartilage healing
    Article Snippet: .. Human Nanog , Oct-4 , Sox-2 , Runx-2 , Osterix , CEBP/α , PPARγ , Sox-9 , and collagen type II (Col-II ) cDNA were amplified by real-time PCR using a SYBR PCR Master Mix Kit (Sigma-Aldrich). ..

    Immunocytochemistry:

    Article Title: Efficient Differentiation of Steroidogenic and Germ-Like Cells from Epigenetically-Related iPSCs Derived from Ovarian Granulosa Cells
    Article Snippet: .. Stem cell status was also assessed by immunocytochemistry (ICC) using commercial stem cell antibodies for mouse Oct4, SSEA-1, and Nanog, and for human OCT4, SSEA-4, TRA-1–60 and TRA-1–81 (Millipore; . .. Differentiation was analyzed by ICC using antibodies to neurofilament [NF, ectoderm], alpha fetoprotein (AFP, endoderm; Santa Cruz, Santa Cruz, CA), and alpha smooth muscle actin (SMA, mesoderm; ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Millipore rabbit anti human nanog
    Generation and characterization of iPSCs from human dermal fibroblasts (HDFs). (A) Schematic diagram represents a protocol to generate iPSCs from HDFs by retroviral transduction. (B) Morphology of HDFs on day 1 post-transduction (i), non ESC-like colonies on day 8 (ii), ESC-like colonies on day 13 (iii), iPS colonies on iHFF plate (iv) and an iPS colony on Matrigel-coated plate (v), scale bar (i) and (iii) = 200 µm, (ii), (iv) and (v) = 500 µm. (C) RT-qPCR analysis of pluripotency- and fibroblast-associated genes in HDF, HDF-iPSC1 and Chula2.hES cells. (D) RT-PCR analysis of pluripotent genes, <t>OCT4</t> and <t>NANOG,</t> of all the established iPS clones, HDF-iPSC2-6, as compared to Chula2.hES cells. (E) Representative immunofluorescent images of pluripotent transcription factors and cell surface antigens of HDF-iPSC1 as compared to Chula2.hES cells, scale bar = 100 µm.
    Rabbit Anti Human Nanog, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human nanog/product/Millipore
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti human nanog - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    87
    Millipore mouse anti human nanog homeobox nanog monoclonal antibody monoclonal antibody cat no mabd24a4
    Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), <t>Nanog</t> <t>homeobox</t> (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.
    Mouse Anti Human Nanog Homeobox Nanog Monoclonal Antibody Monoclonal Antibody Cat No Mabd24a4, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human nanog homeobox nanog monoclonal antibody monoclonal antibody cat no mabd24a4/product/Millipore
    Average 87 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    mouse anti human nanog homeobox nanog monoclonal antibody monoclonal antibody cat no mabd24a4 - by Bioz Stars, 2020-07
    87/100 stars
      Buy from Supplier

    93
    Millipore nanog
    mGriPSCs are pluripotent. Using G4-mESCs as a standard (A-D, I) , mGriPSCs are alkaline phosphatase reactive (E) and express stem cell antigens <t>Oct4</t> (F) , SSEA1 (G) and <t>Nanog</t> (H, I) . mGriPSCS express additional stem cell markers by RT-PCR (I) and are karyotypically normal (J) . mGriPSC express endogenous copies of the introduced reprogramming genes and retroviral [trans] copies are present to varying degrees (K). mGriPSCs form EBs (L) that differentiate into three germ layers—ectodermal neurofilament (M) , and mesodermal SMA (N) , and endoderm alpha-fetoprotein ( O, AFP). Teratomas (P) also show differentiation into three germ layers (Q-S) . Scale bars: A-H 20 μm; L 200 μm; M-O, Q-S 10 μm.
    Nanog, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanog/product/Millipore
    Average 93 stars, based on 72 article reviews
    Price from $9.99 to $1999.99
    nanog - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Generation and characterization of iPSCs from human dermal fibroblasts (HDFs). (A) Schematic diagram represents a protocol to generate iPSCs from HDFs by retroviral transduction. (B) Morphology of HDFs on day 1 post-transduction (i), non ESC-like colonies on day 8 (ii), ESC-like colonies on day 13 (iii), iPS colonies on iHFF plate (iv) and an iPS colony on Matrigel-coated plate (v), scale bar (i) and (iii) = 200 µm, (ii), (iv) and (v) = 500 µm. (C) RT-qPCR analysis of pluripotency- and fibroblast-associated genes in HDF, HDF-iPSC1 and Chula2.hES cells. (D) RT-PCR analysis of pluripotent genes, OCT4 and NANOG, of all the established iPS clones, HDF-iPSC2-6, as compared to Chula2.hES cells. (E) Representative immunofluorescent images of pluripotent transcription factors and cell surface antigens of HDF-iPSC1 as compared to Chula2.hES cells, scale bar = 100 µm.

    Journal: PLoS ONE

    Article Title: Dual Small-Molecule Targeting of SMAD Signaling Stimulates Human Induced Pluripotent Stem Cells toward Neural Lineages

    doi: 10.1371/journal.pone.0106952

    Figure Lengend Snippet: Generation and characterization of iPSCs from human dermal fibroblasts (HDFs). (A) Schematic diagram represents a protocol to generate iPSCs from HDFs by retroviral transduction. (B) Morphology of HDFs on day 1 post-transduction (i), non ESC-like colonies on day 8 (ii), ESC-like colonies on day 13 (iii), iPS colonies on iHFF plate (iv) and an iPS colony on Matrigel-coated plate (v), scale bar (i) and (iii) = 200 µm, (ii), (iv) and (v) = 500 µm. (C) RT-qPCR analysis of pluripotency- and fibroblast-associated genes in HDF, HDF-iPSC1 and Chula2.hES cells. (D) RT-PCR analysis of pluripotent genes, OCT4 and NANOG, of all the established iPS clones, HDF-iPSC2-6, as compared to Chula2.hES cells. (E) Representative immunofluorescent images of pluripotent transcription factors and cell surface antigens of HDF-iPSC1 as compared to Chula2.hES cells, scale bar = 100 µm.

    Article Snippet: The following primary and secondary antibodies were used: mouse anti-human OCT4 (AbD Serotec, UK), rabbit anti-human NANOG (Millipore), Alexa Fluor 488 anti-human TRA-1-60 antibody (BioLegend), mouse anti-human TRA-1-81 (Millipore), mouse anti-human Stage-Specific Embryonic Antigen-4 (SSEA-4) (Millipore), mouse anti-human NESTIN (Millipore), rabbit anti-human TUJ1 (Covance), mouse anti-human α-fetoprotein (Calbiochem), mouse anti-human actin (muscle) clone HHF35 (AbD Serotec), mouse anti-human PAX6 (Millipore), Alexa Fluor 488 γoat anti-mouse IgG (H+L), Alexa Fluor 488 goat anti-rabbit IgG (H+L) and Alexa Fluor 568 goat anti-mouse IgG (H+L) (Life Technologies).

    Techniques: Transduction, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Clone Assay

    Muse-ATs express pluripotent stem cell markers. Immunofluorescence microscopy demonstrates that Muse-AT aggregates, along with individual Muse-AT cells, express characteristic pluripotent stem cell markers, including SSEA3, Oct3/4, Nanog, Sox2, and TRA1-60. Comparatively, ASCs (right panel) derived from the same lipoaspirate under standard conditions (see above, [16] were negative for these pluripotent stem cell markers. Nuclei were stained with DAPI (blue). Original magnification, 600 X.

    Journal: PLoS ONE

    Article Title: Awakened by Cellular Stress: Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue

    doi: 10.1371/journal.pone.0064752

    Figure Lengend Snippet: Muse-ATs express pluripotent stem cell markers. Immunofluorescence microscopy demonstrates that Muse-AT aggregates, along with individual Muse-AT cells, express characteristic pluripotent stem cell markers, including SSEA3, Oct3/4, Nanog, Sox2, and TRA1-60. Comparatively, ASCs (right panel) derived from the same lipoaspirate under standard conditions (see above, [16] were negative for these pluripotent stem cell markers. Nuclei were stained with DAPI (blue). Original magnification, 600 X.

    Article Snippet: The following pluripotent stem cell markers were used: rat anti-human stage-specific embryonic antigen (SSEA3, Millipore, Billerica, MA), mouse anti-human octamer-binding transcription factor 3 and 4 (Oct3/4, Santa Cruz Biotech, Santa Cruz, CA), rabbit anti-human Nanog (Millipore, Billerica, MA), rabbit anti-human SRY-box 2 (Sox2, Millipore, Billerica, MA), and mouse anti-human TRA-1-60 (Abcam, Cambridge, MA); for mesenchymal cell lineages: rabbit anti-human preadipocyte factor 1 (Pref-1, [a.k.a. delta-like 1 homolog (drosophila), DLK1] preadipocyte marker, Santa Cruz Biotech, Santa Cruz, CA); mouse anti-human myosin D (MyoD, myocyte marker, R & D Systems, Minneapolis, MN), and mouse anti-human smooth muscle actin (SMA, myocyte marker, Thermo Scientific, Waltham MA); for endodermal cell lineages: mouse anti-human pan keratin (Santa Cruz, CA); rabbit anti-human α-fetoprotein (Dako, Santa Clara, CA); and mouse anti-human cytokeratin 7 (Millipore, Billerica, MA); and for ectodermal cell lineages: mouse anti-human neuron specific enolase (NSE, Millipore, Billerica, MA); rabbit anti-human glutamate receptor (Abcam, Cambridge, MA); rabbit anti-human NeuroD (Chemicon, Temecula CA); mouse anti-human nestin (Chemicon, Temecula CA); and rabbit anti-human microtubule-associated protein 2 (MAP2, AbDSerotech, Raleigh, NC).

    Techniques: Immunofluorescence, Microscopy, Derivative Assay, Staining

    Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), Nanog homeobox (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.

    Journal: International Journal of Molecular Medicine

    Article Title: X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging

    doi: 10.3892/ijmm.2014.2044

    Figure Lengend Snippet: Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), Nanog homeobox (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.

    Article Snippet: Briefly, for immunofluorescence staining, the hPES-2 cell colonies were incubated with the following primary antibodies against stage-specific embryonic antigens: rat anti-human stage-specific embryonic antigen (SSEA)3 monoclonal antibody (Cat. no. LV1528429), rat anti-human SSEA4 monoclonal antibody (Cat. no. LV1488380), mouse anti-human tumor-rejection antigen (TRA)-1-60 monoclonal antibody (Cat. no. LV1541028), mouse anti-human TRA-1-81 monoclonal antibody (Cat. no. LV1580855); mouse anti-human octamer-binding transcription factor 4 (OCT-4) monoclonal antibody (Cat. no. MAB4419A4) and mouse anti-human Nanog homeobox (NANOG) monoclonal antibody (Cat. no. MABD24A4) (all from Millipore) (all were used at 1:100).

    Techniques: Staining, Expressing, Binding Assay, Amplification, Real-time Polymerase Chain Reaction

    mGriPSCs are pluripotent. Using G4-mESCs as a standard (A-D, I) , mGriPSCs are alkaline phosphatase reactive (E) and express stem cell antigens Oct4 (F) , SSEA1 (G) and Nanog (H, I) . mGriPSCS express additional stem cell markers by RT-PCR (I) and are karyotypically normal (J) . mGriPSC express endogenous copies of the introduced reprogramming genes and retroviral [trans] copies are present to varying degrees (K). mGriPSCs form EBs (L) that differentiate into three germ layers—ectodermal neurofilament (M) , and mesodermal SMA (N) , and endoderm alpha-fetoprotein ( O, AFP). Teratomas (P) also show differentiation into three germ layers (Q-S) . Scale bars: A-H 20 μm; L 200 μm; M-O, Q-S 10 μm.

    Journal: PLoS ONE

    Article Title: Efficient Differentiation of Steroidogenic and Germ-Like Cells from Epigenetically-Related iPSCs Derived from Ovarian Granulosa Cells

    doi: 10.1371/journal.pone.0119275

    Figure Lengend Snippet: mGriPSCs are pluripotent. Using G4-mESCs as a standard (A-D, I) , mGriPSCs are alkaline phosphatase reactive (E) and express stem cell antigens Oct4 (F) , SSEA1 (G) and Nanog (H, I) . mGriPSCS express additional stem cell markers by RT-PCR (I) and are karyotypically normal (J) . mGriPSC express endogenous copies of the introduced reprogramming genes and retroviral [trans] copies are present to varying degrees (K). mGriPSCs form EBs (L) that differentiate into three germ layers—ectodermal neurofilament (M) , and mesodermal SMA (N) , and endoderm alpha-fetoprotein ( O, AFP). Teratomas (P) also show differentiation into three germ layers (Q-S) . Scale bars: A-H 20 μm; L 200 μm; M-O, Q-S 10 μm.

    Article Snippet: Stem cell status was also assessed by immunocytochemistry (ICC) using commercial stem cell antibodies for mouse Oct4, SSEA-1, and Nanog, and for human OCT4, SSEA-4, TRA-1–60 and TRA-1–81 (Millipore; .

    Techniques: Reverse Transcription Polymerase Chain Reaction