Human Myotilin, supplied by OriGene, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum"
Article Title: A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum
Journal: Nature methods
Figure Legend Snippet: Fluorescence imaging of mNeonGreen (mNG) fusion vectors. C-terminal mNG fusion constructs (with respect to the fluorescent protein); for each fusion, the linker amino acid length is indicated after the name of the targeted organelle or fusion partner: ( a ) mNG-Annexin (A4)-C-12 (human; plasma membrane); ( b ) mNG-β-actin-C-18 (human; actin cytoskeleton); ( c ) mNG-β-Catenin-C-20 (mouse; tight junctions); ( d ) mNG-CAAX-C-5 (20-amino acid farnesylation signal from c-Ha-Ras; plasma membrane); (e) mNG-CAF1-C-10 (mouse chromatin assembly factor); ( f ) mNG-Caveolin1-C-10 (human); ( g ) mNG-Endosomes-C-14 (human RhoB GTPase); ( h ) mNG-Fascin-C-10 (human; actin bundling); ( i ) mNG-Fibrillarin-C-7 (human; nucleoli); ( j ) mNG-FilaminA-C-14 (human; cytoskeleton); ( k ) mNG-LAMP1-C-20 (rat; lysosomal membrane glycoprotein 1; lysosomes); ( l ) mNG-Clathrin-C-15 (human, light chain B); ( m ) mNG-Myotilin-C-14 (human; actin filaments); ( n ) mNG-PCNA-C-19 (human; replication foci); ( o ) mNG-Plastin-C-10 (human; actin binding); ( p ) mNG-Rab4a-C-7 (human; endosomes); ( q ) mNG-LC3B-C-7 (rat light chain; autophagosoms; ( r ) mNG-Talin-C-18 (mouse; focal adhesions); ( s ) mNG-Tubulin-C-35 (human; microtubules); ( t ) mNG-ZO1-C-14 (human; tight junctions). The cell line used for expression of C-terminal mNG constructs was Madin-Darby canine kidney (MDCK; ATCC, CCL-34) cells in panels ( c ) and ( t ). HeLa CCL2 (ATCC) cells were used in the remaining panels. Scale bars represent 10 μm.
Techniques Used: Fluorescence, Imaging, Construct, Binding Assay, Expressing
Article Title: A naturally-monomeric infrared fluorescent protein for protein labeling in vivo
Article Snippet: .. To construct the mIFP C-terminal fusions (number of linker amino acids in parenthesis), the following digests were performed: human β-actin (30), NheI and BglII (cDNA source: Clontech, Mountain View, CA, USA; NM_001101.3); CAF1 (22), AgeI and BspEI (mouse chromatin assembly factor; cDNA source: A. Gunjan, Florida State University, Tallahassee, FL, USA; NM_013733.3); human light chain clathrin (27), NheI and BglII (cDNA source: G. Patterson, National Institutes of Health, Bethesda, MD, USA; NM_001834.2); human endosomes (26), NheI and BspEI (human RhoB GTPase; cDNA source: Clontech; NM_004040.2); human fibrillarin (19), AgeI and BspEI (cDNA source: Evrogen, Moscow, Russia; NM_001436.3); H2B (10), BglII and NheI (human histone 2B, cDNA source: G. Patterson, NIH; NM_021058.3); human lamin A/C (30), NheI and BglII (cDNA source: D. Gilbert, Florida State University; NM_170707.2); human lasp1 (22) NheI and BglII (cDNA source: Origene; NM_006148.3);