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human mouse rat monkey histone h2ax serine 139 phosphorylation  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc human mouse rat monkey histone h2ax serine 139 phosphorylation
    Intra-assay Imprecision
    Human Mouse Rat Monkey Histone H2ax Serine 139 Phosphorylation, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human mouse rat monkey histone h2ax serine 139 phosphorylation/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human mouse rat monkey histone h2ax serine 139 phosphorylation - by Bioz Stars, 2024-12
    96/100 stars

    Images

    1) Product Images from "A quasi-quantitative dual multiplexed immunoblot method to simultaneously analyze ATM and H2AX Phosphorylation in human peripheral blood mononuclear cells"

    Article Title: A quasi-quantitative dual multiplexed immunoblot method to simultaneously analyze ATM and H2AX Phosphorylation in human peripheral blood mononuclear cells

    Journal: Oncoscience

    doi:

    Intra-assay Imprecision
    Figure Legend Snippet: Intra-assay Imprecision

    Techniques Used: Intra Assay

    Cellular lysates, prepared from lllltreated (A,D) and 2 Gy irradiated PBMCs (B,E,) from subject B were serially diluted and processed by ATM (A,B) and H2AX (C,D) immunoblot analysis. Fold activation ofATM (C) and H2AX (F) were analyzed as a function ofPBMCs per gel lane.
    Figure Legend Snippet: Cellular lysates, prepared from lllltreated (A,D) and 2 Gy irradiated PBMCs (B,E,) from subject B were serially diluted and processed by ATM (A,B) and H2AX (C,D) immunoblot analysis. Fold activation ofATM (C) and H2AX (F) were analyzed as a function ofPBMCs per gel lane.

    Techniques Used: Irradiation, Western Blot, Activation Assay

    Blood tubes from three subjects were 2 Gy irradiated and PBMCs were isolated and stored at −70°C. Immediately after irradiation and over 46 weeks, PBMC pellets were analyzed for ATM ( A ) and H2AX activation ( B ).
    Figure Legend Snippet: Blood tubes from three subjects were 2 Gy irradiated and PBMCs were isolated and stored at −70°C. Immediately after irradiation and over 46 weeks, PBMC pellets were analyzed for ATM ( A ) and H2AX activation ( B ).

    Techniques Used: Irradiation, Isolation, Activation Assay

    PBMCs were obtained from three patients treated with doxorubicin/ifosfamide. Samples were processed for immunoblot analysis for detection of ATM ( A ) and H2AX activation ( B ). ( C ) Image of H2AX immunoblots in PBMCs from patient#2.
    Figure Legend Snippet: PBMCs were obtained from three patients treated with doxorubicin/ifosfamide. Samples were processed for immunoblot analysis for detection of ATM ( A ) and H2AX activation ( B ). ( C ) Image of H2AX immunoblots in PBMCs from patient#2.

    Techniques Used: Western Blot, Activation Assay



    Similar Products

    human mouse rat monkey histone h2ax serine 139 phosphorylation  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc human mouse rat monkey histone h2ax serine 139 phosphorylation
    Intra-assay Imprecision
    Human Mouse Rat Monkey Histone H2ax Serine 139 Phosphorylation, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human mouse rat monkey histone h2ax serine 139 phosphorylation/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human mouse rat monkey histone h2ax serine 139 phosphorylation - by Bioz Stars, 2024-12
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Intra-assay Imprecision

    Journal: Oncoscience

    Article Title: A quasi-quantitative dual multiplexed immunoblot method to simultaneously analyze ATM and H2AX Phosphorylation in human peripheral blood mononuclear cells

    doi:

    Figure Lengend Snippet: Intra-assay Imprecision

    Article Snippet: The following reagents were used for electrophoresis: glycine, Tween 20, Tris, 10x Tris/Glycine/SDS electrophoresis running buffer, precision plus protein standards (10 – 250 kDa), Tris-HCL and 4-15% TGX Criterion ™ precast (18-well) gels from Bio-Rad Laboratories (Hercules, CA). anti-human ATM serine-1981 phosphorylation antibody, clone EP1890Y, Abcam (Cambridge, MA); purified mouse monoclonal anti-human/mouse pan-ATM antibody, clone 2C1, GeneTex (Irvine, CA); biotinylated rabbit monoclonal anti-human/mouse/rat/monkey histone H2AX serine-139 phosphorylation, clone 20E3, Cell Signaling Technology (Danvers, MA); mouse monoclonal anti-human/mouse/rat histone H2AX antibody, clone 322105, R&D Systems (Minneapolis, MN); rabbit monoclonal anti-human/rabbit/mouse ATR, clone E1S3S, Cell Signaling Technology (Danvers, MA); SMG-1, (Q25), Cell Signaling Technology (Danvers, MA); IRDye 800CW conjugated goat anti-rabbit, and IRDye 680RD conjugated goat anti-mouse LI-COR Biotechnology-US (Lincoln, NE).

    Techniques: Intra Assay

    Cellular lysates, prepared from lllltreated (A,D) and 2 Gy irradiated PBMCs (B,E,) from subject B were serially diluted and processed by ATM (A,B) and H2AX (C,D) immunoblot analysis. Fold activation ofATM (C) and H2AX (F) were analyzed as a function ofPBMCs per gel lane.

    Journal: Oncoscience

    Article Title: A quasi-quantitative dual multiplexed immunoblot method to simultaneously analyze ATM and H2AX Phosphorylation in human peripheral blood mononuclear cells

    doi:

    Figure Lengend Snippet: Cellular lysates, prepared from lllltreated (A,D) and 2 Gy irradiated PBMCs (B,E,) from subject B were serially diluted and processed by ATM (A,B) and H2AX (C,D) immunoblot analysis. Fold activation ofATM (C) and H2AX (F) were analyzed as a function ofPBMCs per gel lane.

    Article Snippet: The following reagents were used for electrophoresis: glycine, Tween 20, Tris, 10x Tris/Glycine/SDS electrophoresis running buffer, precision plus protein standards (10 – 250 kDa), Tris-HCL and 4-15% TGX Criterion ™ precast (18-well) gels from Bio-Rad Laboratories (Hercules, CA). anti-human ATM serine-1981 phosphorylation antibody, clone EP1890Y, Abcam (Cambridge, MA); purified mouse monoclonal anti-human/mouse pan-ATM antibody, clone 2C1, GeneTex (Irvine, CA); biotinylated rabbit monoclonal anti-human/mouse/rat/monkey histone H2AX serine-139 phosphorylation, clone 20E3, Cell Signaling Technology (Danvers, MA); mouse monoclonal anti-human/mouse/rat histone H2AX antibody, clone 322105, R&D Systems (Minneapolis, MN); rabbit monoclonal anti-human/rabbit/mouse ATR, clone E1S3S, Cell Signaling Technology (Danvers, MA); SMG-1, (Q25), Cell Signaling Technology (Danvers, MA); IRDye 800CW conjugated goat anti-rabbit, and IRDye 680RD conjugated goat anti-mouse LI-COR Biotechnology-US (Lincoln, NE).

    Techniques: Irradiation, Western Blot, Activation Assay

    Blood tubes from three subjects were 2 Gy irradiated and PBMCs were isolated and stored at −70°C. Immediately after irradiation and over 46 weeks, PBMC pellets were analyzed for ATM ( A ) and H2AX activation ( B ).

    Journal: Oncoscience

    Article Title: A quasi-quantitative dual multiplexed immunoblot method to simultaneously analyze ATM and H2AX Phosphorylation in human peripheral blood mononuclear cells

    doi:

    Figure Lengend Snippet: Blood tubes from three subjects were 2 Gy irradiated and PBMCs were isolated and stored at −70°C. Immediately after irradiation and over 46 weeks, PBMC pellets were analyzed for ATM ( A ) and H2AX activation ( B ).

    Article Snippet: The following reagents were used for electrophoresis: glycine, Tween 20, Tris, 10x Tris/Glycine/SDS electrophoresis running buffer, precision plus protein standards (10 – 250 kDa), Tris-HCL and 4-15% TGX Criterion ™ precast (18-well) gels from Bio-Rad Laboratories (Hercules, CA). anti-human ATM serine-1981 phosphorylation antibody, clone EP1890Y, Abcam (Cambridge, MA); purified mouse monoclonal anti-human/mouse pan-ATM antibody, clone 2C1, GeneTex (Irvine, CA); biotinylated rabbit monoclonal anti-human/mouse/rat/monkey histone H2AX serine-139 phosphorylation, clone 20E3, Cell Signaling Technology (Danvers, MA); mouse monoclonal anti-human/mouse/rat histone H2AX antibody, clone 322105, R&D Systems (Minneapolis, MN); rabbit monoclonal anti-human/rabbit/mouse ATR, clone E1S3S, Cell Signaling Technology (Danvers, MA); SMG-1, (Q25), Cell Signaling Technology (Danvers, MA); IRDye 800CW conjugated goat anti-rabbit, and IRDye 680RD conjugated goat anti-mouse LI-COR Biotechnology-US (Lincoln, NE).

    Techniques: Irradiation, Isolation, Activation Assay

    PBMCs were obtained from three patients treated with doxorubicin/ifosfamide. Samples were processed for immunoblot analysis for detection of ATM ( A ) and H2AX activation ( B ). ( C ) Image of H2AX immunoblots in PBMCs from patient#2.

    Journal: Oncoscience

    Article Title: A quasi-quantitative dual multiplexed immunoblot method to simultaneously analyze ATM and H2AX Phosphorylation in human peripheral blood mononuclear cells

    doi:

    Figure Lengend Snippet: PBMCs were obtained from three patients treated with doxorubicin/ifosfamide. Samples were processed for immunoblot analysis for detection of ATM ( A ) and H2AX activation ( B ). ( C ) Image of H2AX immunoblots in PBMCs from patient#2.

    Article Snippet: The following reagents were used for electrophoresis: glycine, Tween 20, Tris, 10x Tris/Glycine/SDS electrophoresis running buffer, precision plus protein standards (10 – 250 kDa), Tris-HCL and 4-15% TGX Criterion ™ precast (18-well) gels from Bio-Rad Laboratories (Hercules, CA). anti-human ATM serine-1981 phosphorylation antibody, clone EP1890Y, Abcam (Cambridge, MA); purified mouse monoclonal anti-human/mouse pan-ATM antibody, clone 2C1, GeneTex (Irvine, CA); biotinylated rabbit monoclonal anti-human/mouse/rat/monkey histone H2AX serine-139 phosphorylation, clone 20E3, Cell Signaling Technology (Danvers, MA); mouse monoclonal anti-human/mouse/rat histone H2AX antibody, clone 322105, R&D Systems (Minneapolis, MN); rabbit monoclonal anti-human/rabbit/mouse ATR, clone E1S3S, Cell Signaling Technology (Danvers, MA); SMG-1, (Q25), Cell Signaling Technology (Danvers, MA); IRDye 800CW conjugated goat anti-rabbit, and IRDye 680RD conjugated goat anti-mouse LI-COR Biotechnology-US (Lincoln, NE).

    Techniques: Western Blot, Activation Assay