Human Mouse Rat Monkey Histone H2ax Serine 139 Phosphorylation, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "A quasi-quantitative dual multiplexed immunoblot method to simultaneously analyze ATM and H2AX Phosphorylation in human peripheral blood mononuclear cells"
Article Title: A quasi-quantitative dual multiplexed immunoblot method to simultaneously analyze ATM and H2AX Phosphorylation in human peripheral blood mononuclear cells
Figure Legend Snippet: Intra-assay Imprecision
Techniques Used: Intra Assay
Figure Legend Snippet: Cellular lysates, prepared from lllltreated (A,D) and 2 Gy irradiated PBMCs (B,E,) from subject B were serially diluted and processed by ATM (A,B) and H2AX (C,D) immunoblot analysis. Fold activation ofATM (C) and H2AX (F) were analyzed as a function ofPBMCs per gel lane.
Techniques Used: Irradiation, Western Blot, Activation Assay
Figure Legend Snippet: Blood tubes from three subjects were 2 Gy irradiated and PBMCs were isolated and stored at −70°C. Immediately after irradiation and over 46 weeks, PBMC pellets were analyzed for ATM ( A ) and H2AX activation ( B ).
Techniques Used: Irradiation, Isolation, Activation Assay
Figure Legend Snippet: PBMCs were obtained from three patients treated with doxorubicin/ifosfamide. Samples were processed for immunoblot analysis for detection of ATM ( A ) and H2AX activation ( B ). ( C ) Image of H2AX immunoblots in PBMCs from patient#2.
Techniques Used: Western Blot, Activation Assay