human mmp 2 elisa kit  (Quickzyme)

 
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    Quickzyme human mmp 2 elisa kit
    UV-B irradiation <t>increased</t> <t>matrix</t> <t>metalloproteinase-2</t> <t>(MMP-2)</t> in cell lysates. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, the cells were cultured for another 24 hr under the same culture conditions. Next, cell/matrix layers were collected at 12 ( A ) and 24 ( B ) hr after UV irradiation and analyzed by ELISA. The level of active MMP-2 increased depending on the irradiation level of UV-B. ( A ) The levels of total and active MMP-2 at 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). ( A ) The levels of total and active MMP-2 at 100 and 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). ( B ) The level of active MMP-2 at 100 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). The amounts of total and active MMP-2 at 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). The values for each control sample (0 mJ/cm 2 ) were arbitrarily assigned a value of 1. The results represent the mean ± standard deviation of four independent determinations. ** P < 0.05 versus control.
    Human Mmp 2 Elisa Kit, supplied by Quickzyme, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human mmp 2 elisa kit/product/Quickzyme
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human mmp 2 elisa kit - by Bioz Stars, 2023-11
    86/100 stars

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    1) Product Images from "Matrix Metalloproteinase-2 Activated by Ultraviolet-B Degrades Human Ciliary Zonules In Vitro"

    Article Title: Matrix Metalloproteinase-2 Activated by Ultraviolet-B Degrades Human Ciliary Zonules In Vitro

    Journal: Acta Histochemica et Cytochemica

    doi: 10.1267/ahc.20-00021

    UV-B irradiation increased matrix metalloproteinase-2 (MMP-2) in cell lysates. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, the cells were cultured for another 24 hr under the same culture conditions. Next, cell/matrix layers were collected at 12 ( A ) and 24 ( B ) hr after UV irradiation and analyzed by ELISA. The level of active MMP-2 increased depending on the irradiation level of UV-B. ( A ) The levels of total and active MMP-2 at 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). ( A ) The levels of total and active MMP-2 at 100 and 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). ( B ) The level of active MMP-2 at 100 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). The amounts of total and active MMP-2 at 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). The values for each control sample (0 mJ/cm 2 ) were arbitrarily assigned a value of 1. The results represent the mean ± standard deviation of four independent determinations. ** P < 0.05 versus control.
    Figure Legend Snippet: UV-B irradiation increased matrix metalloproteinase-2 (MMP-2) in cell lysates. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, the cells were cultured for another 24 hr under the same culture conditions. Next, cell/matrix layers were collected at 12 ( A ) and 24 ( B ) hr after UV irradiation and analyzed by ELISA. The level of active MMP-2 increased depending on the irradiation level of UV-B. ( A ) The levels of total and active MMP-2 at 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). ( A ) The levels of total and active MMP-2 at 100 and 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). ( B ) The level of active MMP-2 at 100 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). The amounts of total and active MMP-2 at 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). The values for each control sample (0 mJ/cm 2 ) were arbitrarily assigned a value of 1. The results represent the mean ± standard deviation of four independent determinations. ** P < 0.05 versus control.

    Techniques Used: Irradiation, Cell Culture, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Inhibitory effect by addition of MMP-2 inhibitor. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, 1.5 μM MMP-2 inhibitor was added to the medium for an additional 24 hr. After 24 hr, the cells were simultaneously labeled for fibrillin-1 (green; first panels) and fibrillin-2 (red; second panels). Superimposition of both labels is shown in the third panels. DAPI for nuclear staining is shown in the fourth panels. Bar = 50 μm.
    Figure Legend Snippet: Inhibitory effect by addition of MMP-2 inhibitor. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, 1.5 μM MMP-2 inhibitor was added to the medium for an additional 24 hr. After 24 hr, the cells were simultaneously labeled for fibrillin-1 (green; first panels) and fibrillin-2 (red; second panels). Superimposition of both labels is shown in the third panels. DAPI for nuclear staining is shown in the fourth panels. Bar = 50 μm.

    Techniques Used: Cell Culture, Irradiation, Labeling, Staining

    Inhibitory effect by addition of MMP-2/MMP-9 inhibitor. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, 50 nM MMP-2/MMP-9 inhibitor was added to the medium for an additional 24 hr. After 24 hr, the cells were doubly labeled for fibrillin-1 (green; first panels) and fibrillin-2 (red; second panels). Superimposition of both labels is shown in the third panels. DAPI for nuclear staining is shown in the fourth panels. Bar = 50 μm.
    Figure Legend Snippet: Inhibitory effect by addition of MMP-2/MMP-9 inhibitor. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, 50 nM MMP-2/MMP-9 inhibitor was added to the medium for an additional 24 hr. After 24 hr, the cells were doubly labeled for fibrillin-1 (green; first panels) and fibrillin-2 (red; second panels). Superimposition of both labels is shown in the third panels. DAPI for nuclear staining is shown in the fourth panels. Bar = 50 μm.

    Techniques Used: Cell Culture, Irradiation, Labeling, Staining

    UV-B irradiation increases the level of MMP-2 via p38 activation. Human nonpigmented ciliary epithelial cells were cultured for 24 hr and then the cells pretreated with SB203580 (p38 inhibitor) were irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, the cells were cultured for another 12 and 24 hr under serum-free culture conditions. ( A ) Cell/matrix layers were collected 10 min after UV irradiation and analyzed by Western blot against phosphor-p38. The level of phosphor-p38 treated with SB203580 was inhibited at the same level as the control (0 mJ/cm 2 ). ( B ) Immunofluorescence showed that phosphor-p38 was labeled in the cells at 150 mJ/cm 2 , but not in SB203580-treated cells. Cell/matrix layers were collected at 12 ( C ) and 24 ( D ) hours after UV irradiation and analyzed by ELISA. The active MMP-2 in the SB203580-treated cells was suppressed compared with the SB203580-untreated vehicle cells. The values for each control sample (0 mJ/cm 2 ) without SB203580 were arbitrarily assigned a value of 1. The results represent the mean ± standard deviation of four independent determinations. ** P < 0.05 versus control.
    Figure Legend Snippet: UV-B irradiation increases the level of MMP-2 via p38 activation. Human nonpigmented ciliary epithelial cells were cultured for 24 hr and then the cells pretreated with SB203580 (p38 inhibitor) were irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, the cells were cultured for another 12 and 24 hr under serum-free culture conditions. ( A ) Cell/matrix layers were collected 10 min after UV irradiation and analyzed by Western blot against phosphor-p38. The level of phosphor-p38 treated with SB203580 was inhibited at the same level as the control (0 mJ/cm 2 ). ( B ) Immunofluorescence showed that phosphor-p38 was labeled in the cells at 150 mJ/cm 2 , but not in SB203580-treated cells. Cell/matrix layers were collected at 12 ( C ) and 24 ( D ) hours after UV irradiation and analyzed by ELISA. The active MMP-2 in the SB203580-treated cells was suppressed compared with the SB203580-untreated vehicle cells. The values for each control sample (0 mJ/cm 2 ) without SB203580 were arbitrarily assigned a value of 1. The results represent the mean ± standard deviation of four independent determinations. ** P < 0.05 versus control.

    Techniques Used: Irradiation, Activation Assay, Cell Culture, Western Blot, Immunofluorescence, Labeling, Enzyme-linked Immunosorbent Assay, Standard Deviation

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    Quickzyme human mmp 2 elisa kit
    UV-B irradiation <t>increased</t> <t>matrix</t> <t>metalloproteinase-2</t> <t>(MMP-2)</t> in cell lysates. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, the cells were cultured for another 24 hr under the same culture conditions. Next, cell/matrix layers were collected at 12 ( A ) and 24 ( B ) hr after UV irradiation and analyzed by ELISA. The level of active MMP-2 increased depending on the irradiation level of UV-B. ( A ) The levels of total and active MMP-2 at 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). ( A ) The levels of total and active MMP-2 at 100 and 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). ( B ) The level of active MMP-2 at 100 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). The amounts of total and active MMP-2 at 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). The values for each control sample (0 mJ/cm 2 ) were arbitrarily assigned a value of 1. The results represent the mean ± standard deviation of four independent determinations. ** P < 0.05 versus control.
    Human Mmp 2 Elisa Kit, supplied by Quickzyme, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human mmp 2 elisa kit/product/Quickzyme
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human mmp 2 elisa kit - by Bioz Stars, 2023-11
    86/100 stars
    		  Buy from Supplier

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    UV-B irradiation increased matrix metalloproteinase-2 (MMP-2) in cell lysates. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, the cells were cultured for another 24 hr under the same culture conditions. Next, cell/matrix layers were collected at 12 ( A ) and 24 ( B ) hr after UV irradiation and analyzed by ELISA. The level of active MMP-2 increased depending on the irradiation level of UV-B. ( A ) The levels of total and active MMP-2 at 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). ( A ) The levels of total and active MMP-2 at 100 and 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). ( B ) The level of active MMP-2 at 100 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). The amounts of total and active MMP-2 at 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). The values for each control sample (0 mJ/cm 2 ) were arbitrarily assigned a value of 1. The results represent the mean ± standard deviation of four independent determinations. ** P < 0.05 versus control.

    Journal: Acta Histochemica et Cytochemica

    Article Title: Matrix Metalloproteinase-2 Activated by Ultraviolet-B Degrades Human Ciliary Zonules In Vitro

    doi: 10.1267/ahc.20-00021

    Figure Lengend Snippet: UV-B irradiation increased matrix metalloproteinase-2 (MMP-2) in cell lysates. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, the cells were cultured for another 24 hr under the same culture conditions. Next, cell/matrix layers were collected at 12 ( A ) and 24 ( B ) hr after UV irradiation and analyzed by ELISA. The level of active MMP-2 increased depending on the irradiation level of UV-B. ( A ) The levels of total and active MMP-2 at 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). ( A ) The levels of total and active MMP-2 at 100 and 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). ( B ) The level of active MMP-2 at 100 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). The amounts of total and active MMP-2 at 150 mJ/cm 2 were significantly increased compared to the control (0 mJ/cm 2 ). The values for each control sample (0 mJ/cm 2 ) were arbitrarily assigned a value of 1. The results represent the mean ± standard deviation of four independent determinations. ** P < 0.05 versus control.

    Article Snippet: A human MMP-2 ELISA kit (QuickZyme Biosciences, Leiden, Netherland) was used to measure the protein level of both active-type MMP-2 and total MMP-2 in the cell/matrix of the culture.

    Techniques: Irradiation, Cell Culture, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Inhibitory effect by addition of MMP-2 inhibitor. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, 1.5 μM MMP-2 inhibitor was added to the medium for an additional 24 hr. After 24 hr, the cells were simultaneously labeled for fibrillin-1 (green; first panels) and fibrillin-2 (red; second panels). Superimposition of both labels is shown in the third panels. DAPI for nuclear staining is shown in the fourth panels. Bar = 50 μm.

    Journal: Acta Histochemica et Cytochemica

    Article Title: Matrix Metalloproteinase-2 Activated by Ultraviolet-B Degrades Human Ciliary Zonules In Vitro

    doi: 10.1267/ahc.20-00021

    Figure Lengend Snippet: Inhibitory effect by addition of MMP-2 inhibitor. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, 1.5 μM MMP-2 inhibitor was added to the medium for an additional 24 hr. After 24 hr, the cells were simultaneously labeled for fibrillin-1 (green; first panels) and fibrillin-2 (red; second panels). Superimposition of both labels is shown in the third panels. DAPI for nuclear staining is shown in the fourth panels. Bar = 50 μm.

    Article Snippet: A human MMP-2 ELISA kit (QuickZyme Biosciences, Leiden, Netherland) was used to measure the protein level of both active-type MMP-2 and total MMP-2 in the cell/matrix of the culture.

    Techniques: Cell Culture, Irradiation, Labeling, Staining

    Inhibitory effect by addition of MMP-2/MMP-9 inhibitor. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, 50 nM MMP-2/MMP-9 inhibitor was added to the medium for an additional 24 hr. After 24 hr, the cells were doubly labeled for fibrillin-1 (green; first panels) and fibrillin-2 (red; second panels). Superimposition of both labels is shown in the third panels. DAPI for nuclear staining is shown in the fourth panels. Bar = 50 μm.

    Journal: Acta Histochemica et Cytochemica

    Article Title: Matrix Metalloproteinase-2 Activated by Ultraviolet-B Degrades Human Ciliary Zonules In Vitro

    doi: 10.1267/ahc.20-00021

    Figure Lengend Snippet: Inhibitory effect by addition of MMP-2/MMP-9 inhibitor. Human nonpigmented ciliary epithelial cells were cultured for 4 weeks and then irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, 50 nM MMP-2/MMP-9 inhibitor was added to the medium for an additional 24 hr. After 24 hr, the cells were doubly labeled for fibrillin-1 (green; first panels) and fibrillin-2 (red; second panels). Superimposition of both labels is shown in the third panels. DAPI for nuclear staining is shown in the fourth panels. Bar = 50 μm.

    Article Snippet: A human MMP-2 ELISA kit (QuickZyme Biosciences, Leiden, Netherland) was used to measure the protein level of both active-type MMP-2 and total MMP-2 in the cell/matrix of the culture.

    Techniques: Cell Culture, Irradiation, Labeling, Staining

    UV-B irradiation increases the level of MMP-2 via p38 activation. Human nonpigmented ciliary epithelial cells were cultured for 24 hr and then the cells pretreated with SB203580 (p38 inhibitor) were irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, the cells were cultured for another 12 and 24 hr under serum-free culture conditions. ( A ) Cell/matrix layers were collected 10 min after UV irradiation and analyzed by Western blot against phosphor-p38. The level of phosphor-p38 treated with SB203580 was inhibited at the same level as the control (0 mJ/cm 2 ). ( B ) Immunofluorescence showed that phosphor-p38 was labeled in the cells at 150 mJ/cm 2 , but not in SB203580-treated cells. Cell/matrix layers were collected at 12 ( C ) and 24 ( D ) hours after UV irradiation and analyzed by ELISA. The active MMP-2 in the SB203580-treated cells was suppressed compared with the SB203580-untreated vehicle cells. The values for each control sample (0 mJ/cm 2 ) without SB203580 were arbitrarily assigned a value of 1. The results represent the mean ± standard deviation of four independent determinations. ** P < 0.05 versus control.

    Journal: Acta Histochemica et Cytochemica

    Article Title: Matrix Metalloproteinase-2 Activated by Ultraviolet-B Degrades Human Ciliary Zonules In Vitro

    doi: 10.1267/ahc.20-00021

    Figure Lengend Snippet: UV-B irradiation increases the level of MMP-2 via p38 activation. Human nonpigmented ciliary epithelial cells were cultured for 24 hr and then the cells pretreated with SB203580 (p38 inhibitor) were irradiated with UV-B at levels of 0, 50, 100, and 150 mJ/cm 2 . Then, the cells were cultured for another 12 and 24 hr under serum-free culture conditions. ( A ) Cell/matrix layers were collected 10 min after UV irradiation and analyzed by Western blot against phosphor-p38. The level of phosphor-p38 treated with SB203580 was inhibited at the same level as the control (0 mJ/cm 2 ). ( B ) Immunofluorescence showed that phosphor-p38 was labeled in the cells at 150 mJ/cm 2 , but not in SB203580-treated cells. Cell/matrix layers were collected at 12 ( C ) and 24 ( D ) hours after UV irradiation and analyzed by ELISA. The active MMP-2 in the SB203580-treated cells was suppressed compared with the SB203580-untreated vehicle cells. The values for each control sample (0 mJ/cm 2 ) without SB203580 were arbitrarily assigned a value of 1. The results represent the mean ± standard deviation of four independent determinations. ** P < 0.05 versus control.

    Article Snippet: A human MMP-2 ELISA kit (QuickZyme Biosciences, Leiden, Netherland) was used to measure the protein level of both active-type MMP-2 and total MMP-2 in the cell/matrix of the culture.

    Techniques: Irradiation, Activation Assay, Cell Culture, Western Blot, Immunofluorescence, Labeling, Enzyme-linked Immunosorbent Assay, Standard Deviation