human mmp 2 elisa kit  (Millipore)

 
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    Name:
    Human MMP 2 ELISA Kit
    Description:
    The Human MMP 2 ELISA Enzyme Linked Immunosorbent Assay kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of human MMP 2 in serum plasma cell culture supernatants and urine
    Catalog Number:
    rab0365
    Price:
    None
    Applications:
    Human MMP-2 ELISA Kit has been used in ELISA (enzyme-linked immunosorbent assay).
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    Structured Review

    Millipore human mmp 2 elisa kit
    Human MMP 2 ELISA Kit
    The Human MMP 2 ELISA Enzyme Linked Immunosorbent Assay kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of human MMP 2 in serum plasma cell culture supernatants and urine
    https://www.bioz.com/result/human mmp 2 elisa kit/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human mmp 2 elisa kit - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Detection of early pancreatic ductal adenocarcinoma using thrombospondin-2 and CA19-9 blood markers
    Article Snippet: Therefore, more stringent analysis verified THBS2 sequence in cancer pooled sample. .. ELISA kits for human MMP2 (Millipore), human MMP10 (Ray Biotech), and human Thrombospondin-2 Quantikine (DTSP20, R & D systems) were used as described by the manufacturers’ instructions. ..

    Article Title: Overexpression of miR‐455‐5p affects retinol (vitamin A) absorption by downregulating STRA6 in a nitrofen‐induced CDH with lung hypoplasia rat model, et al. Overexpression of miR‐455‐5p affects retinol (vitamin A) absorption by downregulating STRA6 in a nitrofen‐induced CDH with lung hypoplasia rat model
    Article Snippet: The fetal rats underwent thoracotomy and were dissected under sterile conditions using a Nikon SMZ100 stereomicroscope. .. The left lungs of the fetal rats were collected and immediately placed into cryopreservation tubes, which were frozen on dry ice and stored in liquid nitrogen for total RNA extraction and an enzyme‐linked immunosorbent assay (ELISA). .. Specimens used for morphological analysis or immunofluorescence histochemistry experiments were placed in 10% paraformaldehyde (Beijing Leagene Biotech Co, Ltd, China) for preservation.

    Article Title: Transforming growth factor-?2 increases extracellular matrix proteins in optic nerve head cells via activation of the Smad signaling pathway
    Article Snippet: ELISA immunoassay for fibronectin The conditioned mediums obtained from three ONH astrocytes cell lines and three LC cell lines were centrifuged at 2000 rpm to remove cellular debris. .. A total 50 ul of the conditioned medium was diluted to 150 ul with a dilution buffer, and soluble fibronectin was quantified using a commercially available enzyme-linked immunosorbent assay (ELISA) kit (cat # ECM 300; Chemicon International, Temecula, CA). .. The amounts of soluble fibronectin (ug/ml) were plotted for each treatment using GraphPadPrism 5.

    Article Title: Active Matrix Metalloprotease-9 Is Associated with the Collagen Capsule Surrounding the Madurella mycetomatis Grain in Mycetoma
    Article Snippet: Mayer's hematoxylin (Sigma, Zwijndrecht, The Netherlands) was used for counterstaining. .. ELISA Absolute serum levels of MMP-2 and MMP-9 in serum of M. mycetomatis infected patients (n = 36) and healthy controls (n = 36) were determined utilizing Human MMP-2 and Human MMP-9 enzyme-linked immunosorbent assay (ELISA) kits (cat#: RAB0365, Sigma-Aldrich, Zwijndrecht, The Netherlands; and cat#: KHC3061, Invitrogen, Breda, The Netherlands). .. Human TIMP-1 ELISA kit (Cat#: OK-0163, Assay Biotechnology, Breda, The Netherlands) was used to assess the serum level of TIMP-1 in both study populations (n = 44 for both populations).

    Article Title: Resistin promotes tumor metastasis by down-regulation of miR-519d through the AMPK/p38 signaling pathway in human chondrosarcoma cells
    Article Snippet: ON-TARGETplus MMP2, p38, and control siRNAs were purchased from Dharmacon Research (Lafayette, CO). .. AMPK inhibitors (Ara A and compound C), p38 inhibitor (SB203580), MMP-2 inhibitor, and human MMP-2 ELISA kits were purchased from Calbiochem (San Diego, CA). .. Recombinant human resistin was purchased from PeproTech (Rocky Hill, NJ).

    Article Title: IL-33 enhances glioma cell migration and invasion by upregulation of MMP2 and MMP9 via the ST2-NF-κB pathway
    Article Snippet: Enzyme-linked immunosorbent assay for the determination of MMP2 and MMP9 Cell supernatant was centrifuged at 12,000 g for 15 min at 4°C. .. MMP2 ELISA kits (RAB0365; Sigma, USA) and MMP9 ELISA kits (RAB0372; Sigma, Nanjing, China) were used to measure the levels of protein secretion of MMP2 and MMP9 by glioma cells, respectively, according to the manufacturer's instructions. ..

    RNA Extraction:

    Article Title: Overexpression of miR‐455‐5p affects retinol (vitamin A) absorption by downregulating STRA6 in a nitrofen‐induced CDH with lung hypoplasia rat model, et al. Overexpression of miR‐455‐5p affects retinol (vitamin A) absorption by downregulating STRA6 in a nitrofen‐induced CDH with lung hypoplasia rat model
    Article Snippet: The fetal rats underwent thoracotomy and were dissected under sterile conditions using a Nikon SMZ100 stereomicroscope. .. The left lungs of the fetal rats were collected and immediately placed into cryopreservation tubes, which were frozen on dry ice and stored in liquid nitrogen for total RNA extraction and an enzyme‐linked immunosorbent assay (ELISA). .. Specimens used for morphological analysis or immunofluorescence histochemistry experiments were placed in 10% paraformaldehyde (Beijing Leagene Biotech Co, Ltd, China) for preservation.

    Infection:

    Article Title: Active Matrix Metalloprotease-9 Is Associated with the Collagen Capsule Surrounding the Madurella mycetomatis Grain in Mycetoma
    Article Snippet: Mayer's hematoxylin (Sigma, Zwijndrecht, The Netherlands) was used for counterstaining. .. ELISA Absolute serum levels of MMP-2 and MMP-9 in serum of M. mycetomatis infected patients (n = 36) and healthy controls (n = 36) were determined utilizing Human MMP-2 and Human MMP-9 enzyme-linked immunosorbent assay (ELISA) kits (cat#: RAB0365, Sigma-Aldrich, Zwijndrecht, The Netherlands; and cat#: KHC3061, Invitrogen, Breda, The Netherlands). .. Human TIMP-1 ELISA kit (Cat#: OK-0163, Assay Biotechnology, Breda, The Netherlands) was used to assess the serum level of TIMP-1 in both study populations (n = 44 for both populations).

    other:

    Article Title: Multidrug-Resistant Pseudomonas aeruginosa Evokes Differential Inflammatory Responses in Human Microglial and Retinal Pigment Epithelial Cells
    Article Snippet: The minimum detectable dose of Human MMP-2 was determined to be 3500 pg/mL, and MMP-9 was determined to be 10 pg/mL.

    Acetylene Reduction Assay:

    Article Title: Resistin promotes tumor metastasis by down-regulation of miR-519d through the AMPK/p38 signaling pathway in human chondrosarcoma cells
    Article Snippet: ON-TARGETplus MMP2, p38, and control siRNAs were purchased from Dharmacon Research (Lafayette, CO). .. AMPK inhibitors (Ara A and compound C), p38 inhibitor (SB203580), MMP-2 inhibitor, and human MMP-2 ELISA kits were purchased from Calbiochem (San Diego, CA). .. Recombinant human resistin was purchased from PeproTech (Rocky Hill, NJ).

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  • 93
    Millipore mmp 2 elisa kit
    SMase D binding and cell death of human keratinocytes induced by Loxosceles venom. HaCaT cells (5 × 10 4 /well) were treated with 10 μg of L. laeta venom, preincubated or not with each inhibitor to evaluate cell death, binding of the SMase D to the keratinocyte cell membrane and secretion of <t>MMP-2</t> and MMP-9. Cell death was evaluated after 72 h of incubation by the MTT method. The ability of the toxins to bind to the surface of keratinocytes was analysed by flow cytometry using a horse polyclonal serum against L. intermedia SMases D. Detection of MMP-2 and 9 was performed using Calbiochem MMP- 2 and 9 <t>ELISA</t> Kits. Data are represented as mean ± SEM of duplicates representative of three independent experiments. Statistically analysed by One-Way ANOVA followed by Tukey HSD test. (*)Significant difference in relation to the buffer ( p
    Mmp 2 Elisa Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmp 2 elisa kit/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mmp 2 elisa kit - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    99
    Millipore elisa kits
    Phase 1 validation studies and THBS2 expression in PDAC and other human tumors (A) AUC analysis of blinded <t>ELISA</t> data for the proteinsMMP2, <t>MMP10,</t> and THBS2 in plasma samples from 10 patients with PDAC at various stages of disease compared to 10 healthy controls. (B) Boxplots of THBS2 mRNA expression measured in various human tumors (sample sizes in parentheses) assessed by RNA-seq. Tumors are sorted in order of decreasing median expression of THBS2 mRNA. Of the pancreatic cancer samples from the TGCA database (n=179), we analyzed only PDAC (n=134). All expression values are log2(RSEM values =1) transformed.
    Elisa Kits, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kits/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kits - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

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    SMase D binding and cell death of human keratinocytes induced by Loxosceles venom. HaCaT cells (5 × 10 4 /well) were treated with 10 μg of L. laeta venom, preincubated or not with each inhibitor to evaluate cell death, binding of the SMase D to the keratinocyte cell membrane and secretion of MMP-2 and MMP-9. Cell death was evaluated after 72 h of incubation by the MTT method. The ability of the toxins to bind to the surface of keratinocytes was analysed by flow cytometry using a horse polyclonal serum against L. intermedia SMases D. Detection of MMP-2 and 9 was performed using Calbiochem MMP- 2 and 9 ELISA Kits. Data are represented as mean ± SEM of duplicates representative of three independent experiments. Statistically analysed by One-Way ANOVA followed by Tukey HSD test. (*)Significant difference in relation to the buffer ( p

    Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

    Article Title: Targeting Loxosceles spider Sphingomyelinase D with small-molecule inhibitors as a potential therapeutic approach for loxoscelism

    doi: 10.1080/14756366.2018.1546698

    Figure Lengend Snippet: SMase D binding and cell death of human keratinocytes induced by Loxosceles venom. HaCaT cells (5 × 10 4 /well) were treated with 10 μg of L. laeta venom, preincubated or not with each inhibitor to evaluate cell death, binding of the SMase D to the keratinocyte cell membrane and secretion of MMP-2 and MMP-9. Cell death was evaluated after 72 h of incubation by the MTT method. The ability of the toxins to bind to the surface of keratinocytes was analysed by flow cytometry using a horse polyclonal serum against L. intermedia SMases D. Detection of MMP-2 and 9 was performed using Calbiochem MMP- 2 and 9 ELISA Kits. Data are represented as mean ± SEM of duplicates representative of three independent experiments. Statistically analysed by One-Way ANOVA followed by Tukey HSD test. (*)Significant difference in relation to the buffer ( p

    Article Snippet: Supernatants of the keratinocyte cultures, treated for 72 h with venom in the presence or absence of inhibitors (5 μg), were centrifuged at 405 g for 10 min, and tested for the presence of MMP-2 and MMP-9, using the MMP-2 ELISA Kit (QIA63-1EA) and MMP-9 ELISA Kit (QIA56-1EA), according to the manufacturer’s instructions (Calbiochem®, USA).

    Techniques: Binding Assay, Incubation, MTT Assay, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay

    SMase D binding and cell death of human keratinocytes induced by Loxosceles venom. HaCaT cells (5 × 10 4 /well) were treated with 10 μg of L. laeta venom, preincubated or not with each inhibitor to evaluate cell death, binding of the SMase D to the keratinocyte cell membrane and secretion of MMP-2 and MMP-9. Cell death was evaluated after 72 h of incubation by the MTT method. The ability of the toxins to bind to the surface of keratinocytes was analysed by flow cytometry using a horse polyclonal serum against L. intermedia SMases D. Detection of MMP-2 and 9 was performed using Calbiochem MMP- 2 and 9 ELISA Kits. Data are represented as mean ± SEM of duplicates representative of three independent experiments. Statistically analysed by One-Way ANOVA followed by Tukey HSD test. (*)Significant difference in relation to the buffer ( p

    Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

    Article Title: Targeting Loxosceles spider Sphingomyelinase D with small-molecule inhibitors as a potential therapeutic approach for loxoscelism

    doi: 10.1080/14756366.2018.1546698

    Figure Lengend Snippet: SMase D binding and cell death of human keratinocytes induced by Loxosceles venom. HaCaT cells (5 × 10 4 /well) were treated with 10 μg of L. laeta venom, preincubated or not with each inhibitor to evaluate cell death, binding of the SMase D to the keratinocyte cell membrane and secretion of MMP-2 and MMP-9. Cell death was evaluated after 72 h of incubation by the MTT method. The ability of the toxins to bind to the surface of keratinocytes was analysed by flow cytometry using a horse polyclonal serum against L. intermedia SMases D. Detection of MMP-2 and 9 was performed using Calbiochem MMP- 2 and 9 ELISA Kits. Data are represented as mean ± SEM of duplicates representative of three independent experiments. Statistically analysed by One-Way ANOVA followed by Tukey HSD test. (*)Significant difference in relation to the buffer ( p

    Article Snippet: MMPs dosage in the culture supernatants from human keratinocytes Supernatants of the keratinocyte cultures, treated for 72 h with venom in the presence or absence of inhibitors (5 μg), were centrifuged at 405g for 10 min, and tested for the presence of MMP-2 and MMP-9, using the MMP-2 ELISA Kit (QIA63-1EA) and MMP-9 ELISA Kit (QIA56-1EA), according to the manufacturer’s instructions (Calbiochem®, USA).

    Techniques: Binding Assay, Incubation, MTT Assay, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay

    Phase 1 validation studies and THBS2 expression in PDAC and other human tumors (A) AUC analysis of blinded ELISA data for the proteinsMMP2, MMP10, and THBS2 in plasma samples from 10 patients with PDAC at various stages of disease compared to 10 healthy controls. (B) Boxplots of THBS2 mRNA expression measured in various human tumors (sample sizes in parentheses) assessed by RNA-seq. Tumors are sorted in order of decreasing median expression of THBS2 mRNA. Of the pancreatic cancer samples from the TGCA database (n=179), we analyzed only PDAC (n=134). All expression values are log2(RSEM values =1) transformed.

    Journal: Science translational medicine

    Article Title: Detection of early pancreatic ductal adenocarcinoma using thrombospondin-2 and CA19-9 blood markers

    doi: 10.1126/scitranslmed.aah5583

    Figure Lengend Snippet: Phase 1 validation studies and THBS2 expression in PDAC and other human tumors (A) AUC analysis of blinded ELISA data for the proteinsMMP2, MMP10, and THBS2 in plasma samples from 10 patients with PDAC at various stages of disease compared to 10 healthy controls. (B) Boxplots of THBS2 mRNA expression measured in various human tumors (sample sizes in parentheses) assessed by RNA-seq. Tumors are sorted in order of decreasing median expression of THBS2 mRNA. Of the pancreatic cancer samples from the TGCA database (n=179), we analyzed only PDAC (n=134). All expression values are log2(RSEM values =1) transformed.

    Article Snippet: ELISA kits for human MMP2 (Millipore), human MMP10 (Ray Biotech), and human Thrombospondin-2 Quantikine (DTSP20, R & D systems) were used as described by the manufacturers’ instructions.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, RNA Sequencing Assay, Transformation Assay

    CHME-3 and ARPE-19 cells were challenged with S- PA and MDR- PA , with a MOI of 10:1. At indicated time points, the cells were collected and processed for RNA isolation; cDNA synthesis followed by RT-qPCR. The bar graphs show that there was no significant expression of MMP-2 ( A , D ) or tissue inhibitor of metalloproteinases (TIMP)-1 ( B , E ) in either CHME-3 or ARPE-19 cells. ( C , F ) Significantly elevated expression of MMP-9 was observed after infection of CHME-3 and ARPE-19 cells with MDR- PA and S- PA strains. The data are shown as the mean ± SE from three sets of independent experiments; ** p

    Journal: Microorganisms

    Article Title: Multidrug-Resistant Pseudomonas aeruginosa Evokes Differential Inflammatory Responses in Human Microglial and Retinal Pigment Epithelial Cells

    doi: 10.3390/microorganisms8050735

    Figure Lengend Snippet: CHME-3 and ARPE-19 cells were challenged with S- PA and MDR- PA , with a MOI of 10:1. At indicated time points, the cells were collected and processed for RNA isolation; cDNA synthesis followed by RT-qPCR. The bar graphs show that there was no significant expression of MMP-2 ( A , D ) or tissue inhibitor of metalloproteinases (TIMP)-1 ( B , E ) in either CHME-3 or ARPE-19 cells. ( C , F ) Significantly elevated expression of MMP-9 was observed after infection of CHME-3 and ARPE-19 cells with MDR- PA and S- PA strains. The data are shown as the mean ± SE from three sets of independent experiments; ** p

    Article Snippet: The minimum detectable dose of Human MMP-2 was determined to be 3500 pg/mL, and MMP-9 was determined to be 10 pg/mL.

    Techniques: Isolation, Quantitative RT-PCR, Expressing, Infection