Journal: Frontiers in Immunology
Article Title: Brucella suis Δ mapB outer membrane vesicles as an acellular vaccine against systemic and mucosal B. suis infection
doi: 10.3389/fimmu.2024.1501791
Figure Lengend Snippet: Serum antibodies’ functionality. Mice (n=5/group) were i.m. immunized with wt OMVs (20 µg), Δ mapB OMVs (20 µg), or saline solution (SS) at 0 and 30 days. Blood samples were collected at 23 and 42 days. Antibodies’ capacity to neutralize B. suis 1330 adhesion and invasion to A549 epithelial cells line was performed. Total bacteria adhered to the cell monolayer (A) and internalized bacteria (B) were determined. The ability of anti-OMVs antibodies to facilitate bacterial opsonophagocytosis was also determined. B. suis were preincubated with immune serum as described and added to macrophages for 1 h, then intracellular bacteria were determined for CFU counting (C) . Results are expressed as log CFU/ml. **p<0.01, *p<0.05 vs SS. Results are expressed as mean ± SD of duplicate measurements from three independent.
Article Snippet: After incubation, the bacterial suspension was used to infect a confluent monolayer of A549 human lung epithelial cells (ATCC CCL-185) in 96-well plates (5 x 10 4 cells/well) at a multiplicity of infection (MOI) of 100 for 1 hour at 37°C in 5% CO 2 .
Techniques: Saline, Bacteria