Review



human lung adenocarcinoma epithelial cell line a549  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC human lung adenocarcinoma epithelial cell line a549
    a Fluorescence spectral variations of 1AggI ( c = 0.80 × 10 –5 M) in cell culture medium with 0.05% DMSO. b The corresponding kinetic profiles of the conversion from 1AggI to 1AggII by monitoring the emission intensities at 550 and 600 nm. λ ex = 405 nm. CLSM images of the spontaneous transformation of 1AggI to 1AggII in <t>A549</t> cells at ( c – e ) red channel ( λ ex = 405 nm, λ em = 600–700 nm) and ( f – h ) green channel ( λ ex = 405 nm, λ em = 500–599 nm) for 0, 15, and 30 min. Scale bar: 40 μm. Cell imaging was repeated at least three times with similar results.
    Human Lung Adenocarcinoma Epithelial Cell Line A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human lung adenocarcinoma epithelial cell line a549/product/ATCC
    Average 99 stars, based on 1 article reviews
    human lung adenocarcinoma epithelial cell line a549 - by Bioz Stars, 2025-02
    99/100 stars

    Images

    1) Product Images from "A coopetition-driven strategy of parallel/perpendicular aromatic stacking enabling metastable supramolecular polymerization"

    Article Title: A coopetition-driven strategy of parallel/perpendicular aromatic stacking enabling metastable supramolecular polymerization

    Journal: Nature Communications

    doi: 10.1038/s41467-024-55106-z

    a Fluorescence spectral variations of 1AggI ( c = 0.80 × 10 –5 M) in cell culture medium with 0.05% DMSO. b The corresponding kinetic profiles of the conversion from 1AggI to 1AggII by monitoring the emission intensities at 550 and 600 nm. λ ex = 405 nm. CLSM images of the spontaneous transformation of 1AggI to 1AggII in A549 cells at ( c – e ) red channel ( λ ex = 405 nm, λ em = 600–700 nm) and ( f – h ) green channel ( λ ex = 405 nm, λ em = 500–599 nm) for 0, 15, and 30 min. Scale bar: 40 μm. Cell imaging was repeated at least three times with similar results.
    Figure Legend Snippet: a Fluorescence spectral variations of 1AggI ( c = 0.80 × 10 –5 M) in cell culture medium with 0.05% DMSO. b The corresponding kinetic profiles of the conversion from 1AggI to 1AggII by monitoring the emission intensities at 550 and 600 nm. λ ex = 405 nm. CLSM images of the spontaneous transformation of 1AggI to 1AggII in A549 cells at ( c – e ) red channel ( λ ex = 405 nm, λ em = 600–700 nm) and ( f – h ) green channel ( λ ex = 405 nm, λ em = 500–599 nm) for 0, 15, and 30 min. Scale bar: 40 μm. Cell imaging was repeated at least three times with similar results.

    Techniques Used: Fluorescence, Cell Culture, Transformation Assay, Imaging



    Similar Products

    99
    ATCC human lung adenocarcinoma epithelial cell line a549
    a Fluorescence spectral variations of 1AggI ( c = 0.80 × 10 –5 M) in cell culture medium with 0.05% DMSO. b The corresponding kinetic profiles of the conversion from 1AggI to 1AggII by monitoring the emission intensities at 550 and 600 nm. λ ex = 405 nm. CLSM images of the spontaneous transformation of 1AggI to 1AggII in <t>A549</t> cells at ( c – e ) red channel ( λ ex = 405 nm, λ em = 600–700 nm) and ( f – h ) green channel ( λ ex = 405 nm, λ em = 500–599 nm) for 0, 15, and 30 min. Scale bar: 40 μm. Cell imaging was repeated at least three times with similar results.
    Human Lung Adenocarcinoma Epithelial Cell Line A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human lung adenocarcinoma epithelial cell line a549/product/ATCC
    Average 99 stars, based on 1 article reviews
    human lung adenocarcinoma epithelial cell line a549 - by Bioz Stars, 2025-02
    99/100 stars
      Buy from Supplier

    99
    ATCC a549 human lung adenocarcinoma epithelial cell line
    1,2-NQ-induced genes are related to inflammatory responses in <t>A549</t> cells. ( A ) Volcano plot showing the differentially expressed genes (DEGs) between 1,2-NQ-treated and dimethyl sulfoxide (DMSO)-treated (control) A549 cells. The upregulated genes are shown in red (FDR < 0.01, log 2 -fold change > 0.58), the downregulated genes are shown in blue (FDR < 0.01, log 2 -fold change < −0.58), and unaffected genes are shown in gray. ( B , C ) Gene set enrichment analysis (GSEA) of Hallmark gene sets in A549 cells after 1,2-NQ treatment versus control DMSO. Enrichment plots for ( B ) HALLMARK_E2F_TARGETS and ( C ) HALLMARK_INFLAMMATORY_RESPONSE. ( D , E ) Dot plots showing the results of KEGG pathway enrichment analysis performed for ( D ) upregulated genes and ( E ) downregulated genes in A549 cells after 1,2-NQ treatment versus the DMSO control. NES; normalized enrichment score, FDR; false discovery rate, 1,2-NQ; 1,2-naphthoquinone.
    A549 Human Lung Adenocarcinoma Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a549 human lung adenocarcinoma epithelial cell line/product/ATCC
    Average 99 stars, based on 1 article reviews
    a549 human lung adenocarcinoma epithelial cell line - by Bioz Stars, 2025-02
    99/100 stars
      Buy from Supplier

    99
    ATCC human lung adenocarcinoma a549 epithelial cell line
    Allicin enhanced the proliferation and inhibited the apoptosis of LPS-treated <t>A549</t> cells. (A) CCK-8 assays were performed for measuring the cytotoxicity of LPS in A549 cells. (B) The viability of A549 cells treated with different concentrations of allicin (0, 5, 10, 20, and 40 μg/mL) following exposure to LPS was determined by CCK-8 assays. (C) The proliferation of A549 cells treated with 20 μg/mL allicin following exposure to LPS was determined by EdU assays (magnification: 200 × ). (D) The apoptosis of A549 cells treated with 20 μg/mL allicin following exposure to LPS was measured by flow cytometry. The expression of claudin-4 mRNA (E) and protein levels (F) were determined by qRT-PCR and western blotting, respectively. ∗∗∗ P < 0.001 vs. control; ## P < 0.01, ### P < 0.001 vs. LPS. The original Western blot bands are shown in in the supplemental file.
    Human Lung Adenocarcinoma A549 Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human lung adenocarcinoma a549 epithelial cell line/product/ATCC
    Average 99 stars, based on 1 article reviews
    human lung adenocarcinoma a549 epithelial cell line - by Bioz Stars, 2025-02
    99/100 stars
      Buy from Supplier

    86
    Thermo Fisher human lung adenocarcinoma epithelial cell line a549
    Allicin enhanced the proliferation and inhibited the apoptosis of LPS-treated <t>A549</t> cells. (A) CCK-8 assays were performed for measuring the cytotoxicity of LPS in A549 cells. (B) The viability of A549 cells treated with different concentrations of allicin (0, 5, 10, 20, and 40 μg/mL) following exposure to LPS was determined by CCK-8 assays. (C) The proliferation of A549 cells treated with 20 μg/mL allicin following exposure to LPS was determined by EdU assays (magnification: 200 × ). (D) The apoptosis of A549 cells treated with 20 μg/mL allicin following exposure to LPS was measured by flow cytometry. The expression of claudin-4 mRNA (E) and protein levels (F) were determined by qRT-PCR and western blotting, respectively. ∗∗∗ P < 0.001 vs. control; ## P < 0.01, ### P < 0.001 vs. LPS. The original Western blot bands are shown in in the supplemental file.
    Human Lung Adenocarcinoma Epithelial Cell Line A549, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human lung adenocarcinoma epithelial cell line a549/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    human lung adenocarcinoma epithelial cell line a549 - by Bioz Stars, 2025-02
    86/100 stars
      Buy from Supplier

    99
    ATCC human lung adenocarcinoma alveolar epithelial cell line a549
    Allicin enhanced the proliferation and inhibited the apoptosis of LPS-treated <t>A549</t> cells. (A) CCK-8 assays were performed for measuring the cytotoxicity of LPS in A549 cells. (B) The viability of A549 cells treated with different concentrations of allicin (0, 5, 10, 20, and 40 μg/mL) following exposure to LPS was determined by CCK-8 assays. (C) The proliferation of A549 cells treated with 20 μg/mL allicin following exposure to LPS was determined by EdU assays (magnification: 200 × ). (D) The apoptosis of A549 cells treated with 20 μg/mL allicin following exposure to LPS was measured by flow cytometry. The expression of claudin-4 mRNA (E) and protein levels (F) were determined by qRT-PCR and western blotting, respectively. ∗∗∗ P < 0.001 vs. control; ## P < 0.01, ### P < 0.001 vs. LPS. The original Western blot bands are shown in in the supplemental file.
    Human Lung Adenocarcinoma Alveolar Epithelial Cell Line A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human lung adenocarcinoma alveolar epithelial cell line a549/product/ATCC
    Average 99 stars, based on 1 article reviews
    human lung adenocarcinoma alveolar epithelial cell line a549 - by Bioz Stars, 2025-02
    99/100 stars
      Buy from Supplier

    86
    Thermo Fisher transfection human lung adenocarcinoma epithelial cell line a549
    Allicin enhanced the proliferation and inhibited the apoptosis of LPS-treated <t>A549</t> cells. (A) CCK-8 assays were performed for measuring the cytotoxicity of LPS in A549 cells. (B) The viability of A549 cells treated with different concentrations of allicin (0, 5, 10, 20, and 40 μg/mL) following exposure to LPS was determined by CCK-8 assays. (C) The proliferation of A549 cells treated with 20 μg/mL allicin following exposure to LPS was determined by EdU assays (magnification: 200 × ). (D) The apoptosis of A549 cells treated with 20 μg/mL allicin following exposure to LPS was measured by flow cytometry. The expression of claudin-4 mRNA (E) and protein levels (F) were determined by qRT-PCR and western blotting, respectively. ∗∗∗ P < 0.001 vs. control; ## P < 0.01, ### P < 0.001 vs. LPS. The original Western blot bands are shown in in the supplemental file.
    Transfection Human Lung Adenocarcinoma Epithelial Cell Line A549, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfection human lung adenocarcinoma epithelial cell line a549/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    transfection human lung adenocarcinoma epithelial cell line a549 - by Bioz Stars, 2025-02
    86/100 stars
      Buy from Supplier

    Image Search Results


    a Fluorescence spectral variations of 1AggI ( c = 0.80 × 10 –5 M) in cell culture medium with 0.05% DMSO. b The corresponding kinetic profiles of the conversion from 1AggI to 1AggII by monitoring the emission intensities at 550 and 600 nm. λ ex = 405 nm. CLSM images of the spontaneous transformation of 1AggI to 1AggII in A549 cells at ( c – e ) red channel ( λ ex = 405 nm, λ em = 600–700 nm) and ( f – h ) green channel ( λ ex = 405 nm, λ em = 500–599 nm) for 0, 15, and 30 min. Scale bar: 40 μm. Cell imaging was repeated at least three times with similar results.

    Journal: Nature Communications

    Article Title: A coopetition-driven strategy of parallel/perpendicular aromatic stacking enabling metastable supramolecular polymerization

    doi: 10.1038/s41467-024-55106-z

    Figure Lengend Snippet: a Fluorescence spectral variations of 1AggI ( c = 0.80 × 10 –5 M) in cell culture medium with 0.05% DMSO. b The corresponding kinetic profiles of the conversion from 1AggI to 1AggII by monitoring the emission intensities at 550 and 600 nm. λ ex = 405 nm. CLSM images of the spontaneous transformation of 1AggI to 1AggII in A549 cells at ( c – e ) red channel ( λ ex = 405 nm, λ em = 600–700 nm) and ( f – h ) green channel ( λ ex = 405 nm, λ em = 500–599 nm) for 0, 15, and 30 min. Scale bar: 40 μm. Cell imaging was repeated at least three times with similar results.

    Article Snippet: The human lung adenocarcinoma epithelial cell line A549 (male, CCL-185) was obtained from the Chinese Academy of Science Cell Bank, and B16-F10 cell line (ATCC, CRL-6475) was purchased from Wuhan Pricella Biotechnology Co., Ltd.

    Techniques: Fluorescence, Cell Culture, Transformation Assay, Imaging

    1,2-NQ-induced genes are related to inflammatory responses in A549 cells. ( A ) Volcano plot showing the differentially expressed genes (DEGs) between 1,2-NQ-treated and dimethyl sulfoxide (DMSO)-treated (control) A549 cells. The upregulated genes are shown in red (FDR < 0.01, log 2 -fold change > 0.58), the downregulated genes are shown in blue (FDR < 0.01, log 2 -fold change < −0.58), and unaffected genes are shown in gray. ( B , C ) Gene set enrichment analysis (GSEA) of Hallmark gene sets in A549 cells after 1,2-NQ treatment versus control DMSO. Enrichment plots for ( B ) HALLMARK_E2F_TARGETS and ( C ) HALLMARK_INFLAMMATORY_RESPONSE. ( D , E ) Dot plots showing the results of KEGG pathway enrichment analysis performed for ( D ) upregulated genes and ( E ) downregulated genes in A549 cells after 1,2-NQ treatment versus the DMSO control. NES; normalized enrichment score, FDR; false discovery rate, 1,2-NQ; 1,2-naphthoquinone.

    Journal: International Journal of Molecular Sciences

    Article Title: Epigenetic Regulation of CXC Chemokine Expression by Environmental Electrophiles Through DNA Methyltransferase Inhibition

    doi: 10.3390/ijms252111592

    Figure Lengend Snippet: 1,2-NQ-induced genes are related to inflammatory responses in A549 cells. ( A ) Volcano plot showing the differentially expressed genes (DEGs) between 1,2-NQ-treated and dimethyl sulfoxide (DMSO)-treated (control) A549 cells. The upregulated genes are shown in red (FDR < 0.01, log 2 -fold change > 0.58), the downregulated genes are shown in blue (FDR < 0.01, log 2 -fold change < −0.58), and unaffected genes are shown in gray. ( B , C ) Gene set enrichment analysis (GSEA) of Hallmark gene sets in A549 cells after 1,2-NQ treatment versus control DMSO. Enrichment plots for ( B ) HALLMARK_E2F_TARGETS and ( C ) HALLMARK_INFLAMMATORY_RESPONSE. ( D , E ) Dot plots showing the results of KEGG pathway enrichment analysis performed for ( D ) upregulated genes and ( E ) downregulated genes in A549 cells after 1,2-NQ treatment versus the DMSO control. NES; normalized enrichment score, FDR; false discovery rate, 1,2-NQ; 1,2-naphthoquinone.

    Article Snippet: A549 human lung adenocarcinoma epithelial cell line was obtained from ATCC (CCL-185) and HEK293T human embryonic kidney cell line was obtained from ATCC (CRL-3216).

    Techniques: Control

    Treatment with 1,2-NQ or the DNMT inhibitor 5-aza upregulated the expression of CXC chemokines in A549 cells. ( A – D ) A549 cells were exposed to 10 μM 1,2-NQ or 10 μM 5-aza for the indicated times, and total RNA was extracted. RT–qPCR analysis of ( A ) CXCL1 , ( B ) CXCL3 , ( C ) CXCL5 , and ( D ) CXCL8 expression. Control DMSO is shown in white, 1,2-NQ is shown in gray, and 5-aza is shown in black. The data are expressed as the means ± SEMs. n = 3, N.S.: not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001 versus control DMSO at the same timepoint. Statistical analyses were performed via one-way ANOVA with Tukey’s multiple comparison test. 1,2-NQ; 1,2-naphthoquinone, 5-Aza; 5-aza-2′-deoxycytidine.

    Journal: International Journal of Molecular Sciences

    Article Title: Epigenetic Regulation of CXC Chemokine Expression by Environmental Electrophiles Through DNA Methyltransferase Inhibition

    doi: 10.3390/ijms252111592

    Figure Lengend Snippet: Treatment with 1,2-NQ or the DNMT inhibitor 5-aza upregulated the expression of CXC chemokines in A549 cells. ( A – D ) A549 cells were exposed to 10 μM 1,2-NQ or 10 μM 5-aza for the indicated times, and total RNA was extracted. RT–qPCR analysis of ( A ) CXCL1 , ( B ) CXCL3 , ( C ) CXCL5 , and ( D ) CXCL8 expression. Control DMSO is shown in white, 1,2-NQ is shown in gray, and 5-aza is shown in black. The data are expressed as the means ± SEMs. n = 3, N.S.: not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001 versus control DMSO at the same timepoint. Statistical analyses were performed via one-way ANOVA with Tukey’s multiple comparison test. 1,2-NQ; 1,2-naphthoquinone, 5-Aza; 5-aza-2′-deoxycytidine.

    Article Snippet: A549 human lung adenocarcinoma epithelial cell line was obtained from ATCC (CCL-185) and HEK293T human embryonic kidney cell line was obtained from ATCC (CRL-3216).

    Techniques: Expressing, Quantitative RT-PCR, Control, Comparison

    1,2-NQ induces demethylation of the CXCL8 enhancer region in A549 cells. ( A ) Schematic diagram of the bisulfite target regions. Two different bisulfite targets, −253 to +22 (designated Region 1 within the promoter region) and +35,331 to +35,796 (designated Region 2 within the enhancer region) are indicated. ( B , C ) A549 cells were exposed to 10 μM 1,2-NQ for 72 h, and DNA was extracted for bisulfite sequencing. Methylation levels in ( B ) Region 1 and ( C ) Region 2. The open circles represent demethylated CpG sites, and the closed circles represent methylated CpG sites. ( D ) Methylation levels at the upper cytosine in Region 2 (+35,542). The open bars represent demethylated CpG sites, and the closed bars represent methylated CpG sites. n = 46 (DMSO), n = 56 (1,2-NQ), * p < 0.05, versus control DMSO. Statistical analyses were performed using a two-tailed Fisher’s exact test.

    Journal: International Journal of Molecular Sciences

    Article Title: Epigenetic Regulation of CXC Chemokine Expression by Environmental Electrophiles Through DNA Methyltransferase Inhibition

    doi: 10.3390/ijms252111592

    Figure Lengend Snippet: 1,2-NQ induces demethylation of the CXCL8 enhancer region in A549 cells. ( A ) Schematic diagram of the bisulfite target regions. Two different bisulfite targets, −253 to +22 (designated Region 1 within the promoter region) and +35,331 to +35,796 (designated Region 2 within the enhancer region) are indicated. ( B , C ) A549 cells were exposed to 10 μM 1,2-NQ for 72 h, and DNA was extracted for bisulfite sequencing. Methylation levels in ( B ) Region 1 and ( C ) Region 2. The open circles represent demethylated CpG sites, and the closed circles represent methylated CpG sites. ( D ) Methylation levels at the upper cytosine in Region 2 (+35,542). The open bars represent demethylated CpG sites, and the closed bars represent methylated CpG sites. n = 46 (DMSO), n = 56 (1,2-NQ), * p < 0.05, versus control DMSO. Statistical analyses were performed using a two-tailed Fisher’s exact test.

    Article Snippet: A549 human lung adenocarcinoma epithelial cell line was obtained from ATCC (CCL-185) and HEK293T human embryonic kidney cell line was obtained from ATCC (CRL-3216).

    Techniques: Methylation Sequencing, Methylation, Control, Two Tailed Test

    1,2-NQ promotes p65 recruitment and activates CXCL8 enhancer activity in A549 cells. ( A ) Schematic diagram of a ChIP target region near CXCL8. The ChIP target region, +35,536 to +35,647 (designated ChIP-1 within the enhancer region) is indicated. ( B ) Chromatin from A549 cells exposed to 10 μM 1,2-NQ for 72 h was precipitated with a p65 antibody or control IgG. Binding of p65 to the ChIP-1 region. The data are expressed as the means ± SEMs. n = 3, **** p < 0.0001 versus control DMSO. Statistical analyses were performed via one-way ANOVA with Dunnett’s multiple comparison test. ( C ) Schematic illustration of the used luciferase reporter. ( D ) HEK293T cells were transiently transfected with the luciferase reporter and DNMT3B and exposed to 10 μM 1,2-NQ for 72 h, followed by the luciferase reporter assay. The data are expressed as the means ± SEMs. n = 3, * p < 0.05 versus control DMSO. Statistical analyses were performed using unpaired two-tailed Student’s t tests. 1,2-NQ; 1,2-naphthoquinone.

    Journal: International Journal of Molecular Sciences

    Article Title: Epigenetic Regulation of CXC Chemokine Expression by Environmental Electrophiles Through DNA Methyltransferase Inhibition

    doi: 10.3390/ijms252111592

    Figure Lengend Snippet: 1,2-NQ promotes p65 recruitment and activates CXCL8 enhancer activity in A549 cells. ( A ) Schematic diagram of a ChIP target region near CXCL8. The ChIP target region, +35,536 to +35,647 (designated ChIP-1 within the enhancer region) is indicated. ( B ) Chromatin from A549 cells exposed to 10 μM 1,2-NQ for 72 h was precipitated with a p65 antibody or control IgG. Binding of p65 to the ChIP-1 region. The data are expressed as the means ± SEMs. n = 3, **** p < 0.0001 versus control DMSO. Statistical analyses were performed via one-way ANOVA with Dunnett’s multiple comparison test. ( C ) Schematic illustration of the used luciferase reporter. ( D ) HEK293T cells were transiently transfected with the luciferase reporter and DNMT3B and exposed to 10 μM 1,2-NQ for 72 h, followed by the luciferase reporter assay. The data are expressed as the means ± SEMs. n = 3, * p < 0.05 versus control DMSO. Statistical analyses were performed using unpaired two-tailed Student’s t tests. 1,2-NQ; 1,2-naphthoquinone.

    Article Snippet: A549 human lung adenocarcinoma epithelial cell line was obtained from ATCC (CCL-185) and HEK293T human embryonic kidney cell line was obtained from ATCC (CRL-3216).

    Techniques: Activity Assay, Control, Binding Assay, Comparison, Luciferase, Transfection, Reporter Assay, Two Tailed Test

    1,2-NQ promotes cell proliferation via autocrine CXCL8 signaling in A549 cells. ( A – E ) Cell proliferation was detected using the WST-8 assay. ( A – C ) A549 cells were incubated in serum-free medium for 24 h and then exposed to the indicated concentrations of 1,2-NQ for ( A ) 24, ( B ) 48, or ( C ) 72 h. ( D ) A549 cells were incubated in serum-free medium for 20 h and then exposed to the indicated concentrations of SCH-527123 (a CXCR 1/2 antagonist) for 4 h. The cells were treated with 5 μM 1,2-NQ for 72 h. ( E ) A549 cells were incubated in serum-free medium for 20 h and treated with 100 μM SCH-527123 or DMSO for 4 h. The cells were treated with 5 μM 1,2-NQ for 72 h. The data are expressed as the means ± SEMs. n = 3, N.S.: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus ( A – C ) 0 μM 1,2-NQ or ( D , E ) 5 μM 1,2-NQ alone. Statistical analyses were performed via one-way ANOVA with Dunnett’s multiple comparison test. 1,2-NQ; 1,2-naphthoquinone, SCH; SCH-527123.

    Journal: International Journal of Molecular Sciences

    Article Title: Epigenetic Regulation of CXC Chemokine Expression by Environmental Electrophiles Through DNA Methyltransferase Inhibition

    doi: 10.3390/ijms252111592

    Figure Lengend Snippet: 1,2-NQ promotes cell proliferation via autocrine CXCL8 signaling in A549 cells. ( A – E ) Cell proliferation was detected using the WST-8 assay. ( A – C ) A549 cells were incubated in serum-free medium for 24 h and then exposed to the indicated concentrations of 1,2-NQ for ( A ) 24, ( B ) 48, or ( C ) 72 h. ( D ) A549 cells were incubated in serum-free medium for 20 h and then exposed to the indicated concentrations of SCH-527123 (a CXCR 1/2 antagonist) for 4 h. The cells were treated with 5 μM 1,2-NQ for 72 h. ( E ) A549 cells were incubated in serum-free medium for 20 h and treated with 100 μM SCH-527123 or DMSO for 4 h. The cells were treated with 5 μM 1,2-NQ for 72 h. The data are expressed as the means ± SEMs. n = 3, N.S.: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus ( A – C ) 0 μM 1,2-NQ or ( D , E ) 5 μM 1,2-NQ alone. Statistical analyses were performed via one-way ANOVA with Dunnett’s multiple comparison test. 1,2-NQ; 1,2-naphthoquinone, SCH; SCH-527123.

    Article Snippet: A549 human lung adenocarcinoma epithelial cell line was obtained from ATCC (CCL-185) and HEK293T human embryonic kidney cell line was obtained from ATCC (CRL-3216).

    Techniques: Incubation, Comparison

    Allicin enhanced the proliferation and inhibited the apoptosis of LPS-treated A549 cells. (A) CCK-8 assays were performed for measuring the cytotoxicity of LPS in A549 cells. (B) The viability of A549 cells treated with different concentrations of allicin (0, 5, 10, 20, and 40 μg/mL) following exposure to LPS was determined by CCK-8 assays. (C) The proliferation of A549 cells treated with 20 μg/mL allicin following exposure to LPS was determined by EdU assays (magnification: 200 × ). (D) The apoptosis of A549 cells treated with 20 μg/mL allicin following exposure to LPS was measured by flow cytometry. The expression of claudin-4 mRNA (E) and protein levels (F) were determined by qRT-PCR and western blotting, respectively. ∗∗∗ P < 0.001 vs. control; ## P < 0.01, ### P < 0.001 vs. LPS. The original Western blot bands are shown in in the supplemental file.

    Journal: Heliyon

    Article Title: Role of miR-455-3p in the alleviation of LPS-induced acute lung injury by allicin

    doi: 10.1016/j.heliyon.2024.e39338

    Figure Lengend Snippet: Allicin enhanced the proliferation and inhibited the apoptosis of LPS-treated A549 cells. (A) CCK-8 assays were performed for measuring the cytotoxicity of LPS in A549 cells. (B) The viability of A549 cells treated with different concentrations of allicin (0, 5, 10, 20, and 40 μg/mL) following exposure to LPS was determined by CCK-8 assays. (C) The proliferation of A549 cells treated with 20 μg/mL allicin following exposure to LPS was determined by EdU assays (magnification: 200 × ). (D) The apoptosis of A549 cells treated with 20 μg/mL allicin following exposure to LPS was measured by flow cytometry. The expression of claudin-4 mRNA (E) and protein levels (F) were determined by qRT-PCR and western blotting, respectively. ∗∗∗ P < 0.001 vs. control; ## P < 0.01, ### P < 0.001 vs. LPS. The original Western blot bands are shown in in the supplemental file.

    Article Snippet: The human lung adenocarcinoma A549 epithelial cell line was purchased from American Type Culture Collection (ATCC; Manassas, VA, USA) and cultured in Dulbecco's modified Eagle medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 % fetal bovine serum (FBS, Thermo Fisher Scientific) at 37 °C in a humidified atmosphere of 5 % CO 2 and 95 % air.

    Techniques: CCK-8 Assay, Flow Cytometry, Expressing, Quantitative RT-PCR, Western Blot, Control

    Claudin-4 is a direct target of miR-455. (A) The predicted binding sequence of miR-455 in the 3′-UTR of claudin-4. (B) The activity of luciferase was determined by dual-luciferase reporter assays. (C) The viability of A549 cells following transfection with NC, miR-455 mimics, or miR-455 inhibitors was determined by CCK-8 assays. (D) The proliferation of A549 cells following transfection was determined by EdU assays (magnification: 200 × ). (E) The apoptosis of A549 cells following transfection was determined by flow cytometry. The expression of claudin-4 mRNA (F) and protein levels of claudin-4 (G) following the transfection A549 cells was determined by qRT-PCR and western blotting, respectively. (H) The expression of miR-455 following the transfection of A549 cells was determined by qRT-PCR. (I) Expression of miR-455 in A549 cells treated with allicin following LPS exposure. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. NC; ## P < 0.01, ### P < 0.001 vs. NC. The original Western blot bands are shown in in the supplemental file.

    Journal: Heliyon

    Article Title: Role of miR-455-3p in the alleviation of LPS-induced acute lung injury by allicin

    doi: 10.1016/j.heliyon.2024.e39338

    Figure Lengend Snippet: Claudin-4 is a direct target of miR-455. (A) The predicted binding sequence of miR-455 in the 3′-UTR of claudin-4. (B) The activity of luciferase was determined by dual-luciferase reporter assays. (C) The viability of A549 cells following transfection with NC, miR-455 mimics, or miR-455 inhibitors was determined by CCK-8 assays. (D) The proliferation of A549 cells following transfection was determined by EdU assays (magnification: 200 × ). (E) The apoptosis of A549 cells following transfection was determined by flow cytometry. The expression of claudin-4 mRNA (F) and protein levels of claudin-4 (G) following the transfection A549 cells was determined by qRT-PCR and western blotting, respectively. (H) The expression of miR-455 following the transfection of A549 cells was determined by qRT-PCR. (I) Expression of miR-455 in A549 cells treated with allicin following LPS exposure. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. NC; ## P < 0.01, ### P < 0.001 vs. NC. The original Western blot bands are shown in in the supplemental file.

    Article Snippet: The human lung adenocarcinoma A549 epithelial cell line was purchased from American Type Culture Collection (ATCC; Manassas, VA, USA) and cultured in Dulbecco's modified Eagle medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 % fetal bovine serum (FBS, Thermo Fisher Scientific) at 37 °C in a humidified atmosphere of 5 % CO 2 and 95 % air.

    Techniques: Binding Assay, Sequencing, Activity Assay, Luciferase, Transfection, CCK-8 Assay, Flow Cytometry, Expressing, Quantitative RT-PCR, Western Blot

    MiR-455 overexpression partially reversed the protective effect of allicin on LPS-treated A549 cells. (A) The viability of A549 cells was measured by CCK-8 assays. (B) The proliferation of A549 cells was detected by EdU assays (magnification: 200 × ). The expression of claudin-4 mRNA (C) and protein levels (D) in A549 cells were determined by RT-PCR and western blotting, respectively. (E) The expression of miR-455 in A549 cells was determined by qRT-PCR. ∗∗∗ P < 0.001 vs. NS; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. ALI. The original Western blot bands are shown in in the supplemental file.

    Journal: Heliyon

    Article Title: Role of miR-455-3p in the alleviation of LPS-induced acute lung injury by allicin

    doi: 10.1016/j.heliyon.2024.e39338

    Figure Lengend Snippet: MiR-455 overexpression partially reversed the protective effect of allicin on LPS-treated A549 cells. (A) The viability of A549 cells was measured by CCK-8 assays. (B) The proliferation of A549 cells was detected by EdU assays (magnification: 200 × ). The expression of claudin-4 mRNA (C) and protein levels (D) in A549 cells were determined by RT-PCR and western blotting, respectively. (E) The expression of miR-455 in A549 cells was determined by qRT-PCR. ∗∗∗ P < 0.001 vs. NS; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. ALI. The original Western blot bands are shown in in the supplemental file.

    Article Snippet: The human lung adenocarcinoma A549 epithelial cell line was purchased from American Type Culture Collection (ATCC; Manassas, VA, USA) and cultured in Dulbecco's modified Eagle medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 % fetal bovine serum (FBS, Thermo Fisher Scientific) at 37 °C in a humidified atmosphere of 5 % CO 2 and 95 % air.

    Techniques: Over Expression, CCK-8 Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Quantitative RT-PCR