low passage hacat keratinocyte cell line  (ATCC)

Journal: Gels

Article Title: Titanium-Integrated Magnetic Silica Aerogels via Microfluidic Synthesis for Pesticide Removal from Water

doi: 10.3390/gels12040309

Figure Lengend Snippet: Comprehensive evaluation of the biological effects induced by the AG-Ti@Fe 3 O 4 -SA composite aerogel and Fe 3 O 4 -SA nanoparticles in HaCaT ( a , b ) and HEK293 ( c , d ) cells after 48 h of exposure. Biocompatibility was assessed by measuring cell viability (MTT assay), LDH release, and nitric oxide (NO) production, while oxidative stress was evaluated by quantifying reduced glutathione (GSH), malondialdehyde (MDA), and intracellular reactive oxygen species (ROS). The results are expressed as mean ± standard deviation of three independent experiments and presented relative to controls. Statistical significance is indicated as ** p < 0.01 and *** p < 0.001 vs. control, and ## p < 0.01 vs. Fe 3 O 4 -SA nanoparticles.

Article Snippet: The HaCaT human keratinocyte cell line was purchased from Cell Lines Service (CLS, Germany, cat. no. 300493).

Techniques: MTT Assay, Standard Deviation, Control

Journal: Gels

Article Title: Titanium-Integrated Magnetic Silica Aerogels via Microfluidic Synthesis for Pesticide Removal from Water

doi: 10.3390/gels12040309

Figure Lengend Snippet: Representative fluorescence images of HaCaT and HEK293 cells after 48 h incubation with the AG-Ti@Fe 3 O 4 -SA composite aerogel and Fe 3 O 4 -SA nanoparticles: ( a ) Live/dead staining was performed using calcein-AM (green fluorescence showing viable cells) and ethidium homodimer-1 (red fluorescence showing non-viable cells). ( b ) The actin cytoskeleton was visualized by FITC–phalloidin staining (green fluorescence), which highlighted F-actin. Scale bar: 50 µm for all images.

Article Snippet: The HaCaT human keratinocyte cell line was purchased from Cell Lines Service (CLS, Germany, cat. no. 300493).

Techniques: Fluorescence, Incubation, Staining