human gnrh r sirna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher human gnrh r sirna
    Validation of the detection of <t>GnRH-R</t> in immunoblots. A. Representative immunoblots of GnRH-R detection (upper panel) after 48 h transfection with the Human cDNA clone pCMV6-XL5/GNRH-R in 16HBE14o − (1) and CFBE41o − (2) cells. pCMV6-XL5 empty plasmid was used as a control. B. The densitometric analysis after normalization by G3PDH expression and comparison with the controls, indicate that the GnRH-R expression in significantly increased, (n = 5). C. Representative immunoblots of GnRH-R detection after 72 h transfection with a siGENOME individual duplex targeting GnRH-R in 16HBE14o − (1) and CFBE41o − (2) cells. siGENOME Non-Targeting was used as control. A decreased expression of GnRH-R is observed in both cell types. D. The densitometric analysis after normalization by G3PDH expression and comparison with the controls, indicate that the GnRH-R expression is significantly decreased in 16HBE14o − (1) and CFBE41o − (2) cells (n = 7) in the presence of <t>siRNA.</t>
    Human Gnrh R Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human gnrh r sirna/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human gnrh r sirna - by Bioz Stars, 2020-09
    86/100 stars

    Related Products / Commonly Used Together

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    Images

    1) Product Images from "Improvement of Chloride Transport Defect by Gonadotropin-Releasing Hormone (GnRH) in Cystic Fibrosis Epithelial Cells"

    Article Title: Improvement of Chloride Transport Defect by Gonadotropin-Releasing Hormone (GnRH) in Cystic Fibrosis Epithelial Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0088964

    Validation of the detection of GnRH-R in immunoblots. A. Representative immunoblots of GnRH-R detection (upper panel) after 48 h transfection with the Human cDNA clone pCMV6-XL5/GNRH-R in 16HBE14o − (1) and CFBE41o − (2) cells. pCMV6-XL5 empty plasmid was used as a control. B. The densitometric analysis after normalization by G3PDH expression and comparison with the controls, indicate that the GnRH-R expression in significantly increased, (n = 5). C. Representative immunoblots of GnRH-R detection after 72 h transfection with a siGENOME individual duplex targeting GnRH-R in 16HBE14o − (1) and CFBE41o − (2) cells. siGENOME Non-Targeting was used as control. A decreased expression of GnRH-R is observed in both cell types. D. The densitometric analysis after normalization by G3PDH expression and comparison with the controls, indicate that the GnRH-R expression is significantly decreased in 16HBE14o − (1) and CFBE41o − (2) cells (n = 7) in the presence of siRNA.
    Figure Legend Snippet: Validation of the detection of GnRH-R in immunoblots. A. Representative immunoblots of GnRH-R detection (upper panel) after 48 h transfection with the Human cDNA clone pCMV6-XL5/GNRH-R in 16HBE14o − (1) and CFBE41o − (2) cells. pCMV6-XL5 empty plasmid was used as a control. B. The densitometric analysis after normalization by G3PDH expression and comparison with the controls, indicate that the GnRH-R expression in significantly increased, (n = 5). C. Representative immunoblots of GnRH-R detection after 72 h transfection with a siGENOME individual duplex targeting GnRH-R in 16HBE14o − (1) and CFBE41o − (2) cells. siGENOME Non-Targeting was used as control. A decreased expression of GnRH-R is observed in both cell types. D. The densitometric analysis after normalization by G3PDH expression and comparison with the controls, indicate that the GnRH-R expression is significantly decreased in 16HBE14o − (1) and CFBE41o − (2) cells (n = 7) in the presence of siRNA.

    Techniques Used: Western Blot, Transfection, Plasmid Preparation, Expressing

    Related Articles

    Transfection:

    Article Title: Improvement of Chloride Transport Defect by Gonadotropin-Releasing Hormone (GnRH) in Cystic Fibrosis Epithelial Cells
    Article Snippet: .. Transfection For GnRH-R overexpression and inhibition, cells were transfected using Lipofectamine 2000 (Life Technologies Corporation, Carlsbad, CA, USA), according to the manufacturer’s instructions, with either the human cDNA clone pCMV6-XL5/GNRH-R (OriGene Technologies Inc., Rockville, MD, USA) or the human GnRH-R siRNA (5′-GGAAUUUGGUAUUGGUUUG-3′, siGENOME individual duplex (Thermo Fisher Scientific Inc., Waltham, MA, USA). .. The scrambled control siRNA was siGENOME Non-Targeting (Thermo Fisher Scientific Inc.).

    Over Expression:

    Article Title: Improvement of Chloride Transport Defect by Gonadotropin-Releasing Hormone (GnRH) in Cystic Fibrosis Epithelial Cells
    Article Snippet: .. Transfection For GnRH-R overexpression and inhibition, cells were transfected using Lipofectamine 2000 (Life Technologies Corporation, Carlsbad, CA, USA), according to the manufacturer’s instructions, with either the human cDNA clone pCMV6-XL5/GNRH-R (OriGene Technologies Inc., Rockville, MD, USA) or the human GnRH-R siRNA (5′-GGAAUUUGGUAUUGGUUUG-3′, siGENOME individual duplex (Thermo Fisher Scientific Inc., Waltham, MA, USA). .. The scrambled control siRNA was siGENOME Non-Targeting (Thermo Fisher Scientific Inc.).

    Inhibition:

    Article Title: Improvement of Chloride Transport Defect by Gonadotropin-Releasing Hormone (GnRH) in Cystic Fibrosis Epithelial Cells
    Article Snippet: .. Transfection For GnRH-R overexpression and inhibition, cells were transfected using Lipofectamine 2000 (Life Technologies Corporation, Carlsbad, CA, USA), according to the manufacturer’s instructions, with either the human cDNA clone pCMV6-XL5/GNRH-R (OriGene Technologies Inc., Rockville, MD, USA) or the human GnRH-R siRNA (5′-GGAAUUUGGUAUUGGUUUG-3′, siGENOME individual duplex (Thermo Fisher Scientific Inc., Waltham, MA, USA). .. The scrambled control siRNA was siGENOME Non-Targeting (Thermo Fisher Scientific Inc.).

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    Thermo Fisher human gnrh r sirna
    Validation of the detection of <t>GnRH-R</t> in immunoblots. A. Representative immunoblots of GnRH-R detection (upper panel) after 48 h transfection with the Human cDNA clone pCMV6-XL5/GNRH-R in 16HBE14o − (1) and CFBE41o − (2) cells. pCMV6-XL5 empty plasmid was used as a control. B. The densitometric analysis after normalization by G3PDH expression and comparison with the controls, indicate that the GnRH-R expression in significantly increased, (n = 5). C. Representative immunoblots of GnRH-R detection after 72 h transfection with a siGENOME individual duplex targeting GnRH-R in 16HBE14o − (1) and CFBE41o − (2) cells. siGENOME Non-Targeting was used as control. A decreased expression of GnRH-R is observed in both cell types. D. The densitometric analysis after normalization by G3PDH expression and comparison with the controls, indicate that the GnRH-R expression is significantly decreased in 16HBE14o − (1) and CFBE41o − (2) cells (n = 7) in the presence of <t>siRNA.</t>
    Human Gnrh R Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human gnrh r sirna/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human gnrh r sirna - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

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    Validation of the detection of GnRH-R in immunoblots. A. Representative immunoblots of GnRH-R detection (upper panel) after 48 h transfection with the Human cDNA clone pCMV6-XL5/GNRH-R in 16HBE14o − (1) and CFBE41o − (2) cells. pCMV6-XL5 empty plasmid was used as a control. B. The densitometric analysis after normalization by G3PDH expression and comparison with the controls, indicate that the GnRH-R expression in significantly increased, (n = 5). C. Representative immunoblots of GnRH-R detection after 72 h transfection with a siGENOME individual duplex targeting GnRH-R in 16HBE14o − (1) and CFBE41o − (2) cells. siGENOME Non-Targeting was used as control. A decreased expression of GnRH-R is observed in both cell types. D. The densitometric analysis after normalization by G3PDH expression and comparison with the controls, indicate that the GnRH-R expression is significantly decreased in 16HBE14o − (1) and CFBE41o − (2) cells (n = 7) in the presence of siRNA.

    Journal: PLoS ONE

    Article Title: Improvement of Chloride Transport Defect by Gonadotropin-Releasing Hormone (GnRH) in Cystic Fibrosis Epithelial Cells

    doi: 10.1371/journal.pone.0088964

    Figure Lengend Snippet: Validation of the detection of GnRH-R in immunoblots. A. Representative immunoblots of GnRH-R detection (upper panel) after 48 h transfection with the Human cDNA clone pCMV6-XL5/GNRH-R in 16HBE14o − (1) and CFBE41o − (2) cells. pCMV6-XL5 empty plasmid was used as a control. B. The densitometric analysis after normalization by G3PDH expression and comparison with the controls, indicate that the GnRH-R expression in significantly increased, (n = 5). C. Representative immunoblots of GnRH-R detection after 72 h transfection with a siGENOME individual duplex targeting GnRH-R in 16HBE14o − (1) and CFBE41o − (2) cells. siGENOME Non-Targeting was used as control. A decreased expression of GnRH-R is observed in both cell types. D. The densitometric analysis after normalization by G3PDH expression and comparison with the controls, indicate that the GnRH-R expression is significantly decreased in 16HBE14o − (1) and CFBE41o − (2) cells (n = 7) in the presence of siRNA.

    Article Snippet: Transfection For GnRH-R overexpression and inhibition, cells were transfected using Lipofectamine 2000 (Life Technologies Corporation, Carlsbad, CA, USA), according to the manufacturer’s instructions, with either the human cDNA clone pCMV6-XL5/GNRH-R (OriGene Technologies Inc., Rockville, MD, USA) or the human GnRH-R siRNA (5′-GGAAUUUGGUAUUGGUUUG-3′, siGENOME individual duplex (Thermo Fisher Scientific Inc., Waltham, MA, USA).

    Techniques: Western Blot, Transfection, Plasmid Preparation, Expressing