Journal: Scientific Reports

Article Title: Pan-cancer analysis identifies ADAM12 as a prognostic biomarker and indicator of immune infiltration in glioma

doi: 10.1038/s41598-025-90121-0

Figure Lengend Snippet: The expression levels of ADAM12 in various tumors. ( A ) ADAM12 gene expression in Breast invasive carcinoma (BRCA), Glioblastoma-Lower Grade Glioma(GBMLGG), Bladder Cancer (BLCA), Esophageal Carcinoma (ESCA), Kidney Chromophobe (KICH),Kidney Clear Cell Carcinoma (KIRC), Kidney Papillary Cell Carcinoma (KIRP), Ovarian Cancer (OV), Lung Adenocarcinoma (LUAD), Lung Squamous Cell Carcinoma (LUSC), Adrenocortical Cancer (ACC) using GEPIA2. ( B – L ) The correlation between ADAM12 expression and different cancer stages/histological grades of tumors. ( B ) GBMLGG, ( C ) GBM, ( D ) BRCA, ( E ) KIRC, ( F ) KIRP, ( G ) KICH, ( H ) BLCA, ( I ) LIAD, ( J ) LIHC, ( K ) ACC, ( L ) OV.

Article Snippet: Human glioblastoma multiforme (GBM) cell lines (U87MG and U251MG) were sourced from Procell Life Science & Technology Co., Ltd (Wuhan, China) and verified via short tandem repeat (STR) profiling.

Techniques: Expressing

Journal: Pharmaceuticals

Article Title: N-Glycoside of Indolo[2,3- a ]pyrrolo[3,4- c ]carbazole LCS1269 Exerts Anti-Glioblastoma Effects by G2 Cell Cycle Arrest and CDK1 Activity Modulation: Molecular Docking Studies, Biological Investigations, and ADMET Prediction

doi: 10.3390/ph17121642

Figure Lengend Snippet: Inhibitory effect of LCS1269 on cell viability of human glioblastoma cell lines and patient-derived glioblastoma cell cultures. ( A – C ) The viability of three glioblastoma cell lines treated with the indicated concentrations of LCS1269 for 72 h. ( D ) IC 50 values of LCS1269 for U87, U251, and T98G cell lines. ( E – G ) LCS1269 treatment for 72 h reduces the viability of three patient-derived glioblastoma cell cultures in a dose-dependent manner. ( H ) IC 50 values of LCS1269 for Gbl1, Gbl2, and Gbl3 cell cultures. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 as compared with the untreated cells.

Article Snippet: The human glioblastoma multiforme (GBM) cell lines U87, U251, and T98G were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Derivative Assay

Journal: Pharmaceuticals

Article Title: N-Glycoside of Indolo[2,3- a ]pyrrolo[3,4- c ]carbazole LCS1269 Exerts Anti-Glioblastoma Effects by G2 Cell Cycle Arrest and CDK1 Activity Modulation: Molecular Docking Studies, Biological Investigations, and ADMET Prediction

doi: 10.3390/ph17121642

Figure Lengend Snippet: Inhibitory effect of conventional anti-glioblastoma drug temozolomide on cell viability of human glioblastoma cell lines and patient-derived glioblastoma cell cultures and therapeutic potency comparison of temozolomide and LCS1269. ( A – C ) The viability of three glioblastoma cell lines treated with the indicated concentrations of temozolomide for 72 h. ( D ) IC 50 values comparison of temozolomide and LCS1269 for U87, U251, and T98G cell lines. ( E – G ) temozolomide treatment for 72 h reduces the viability of three patient-derived glioblastoma cell cultures in a dose-dependent manner. ( H ) IC 50 values comparison of temozolomide and LCS1269 for Gbl1, Gbl2, and Gbl3 cell cultures. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 as compared with the untreated cells.

Article Snippet: The human glioblastoma multiforme (GBM) cell lines U87, U251, and T98G were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Derivative Assay, Comparison

Journal: Pharmaceuticals

Article Title: N-Glycoside of Indolo[2,3- a ]pyrrolo[3,4- c ]carbazole LCS1269 Exerts Anti-Glioblastoma Effects by G2 Cell Cycle Arrest and CDK1 Activity Modulation: Molecular Docking Studies, Biological Investigations, and ADMET Prediction

doi: 10.3390/ph17121642

Figure Lengend Snippet: Antitumor efficacy of LCS1269 against xenografts in U87-bearing nude mice ( n = 5). ( A ) Representative image of xenograft-bearing animals ( upper panel ) and extirpated tumors ( lower panel ) from control group. ( B ) Representative image of xenograft-bearing animals ( upper panel ) and extirpated tumors ( lower panel ) from LCS1269-treated group. ( C ) Body weight changes in nude mice from control and LCS1269-treated groups. ( D ) Changes in U87 xenograft volumes in control and LCS1269-treated groups of nude mice. Data are presented as mean ± SD.

Article Snippet: The human glioblastoma multiforme (GBM) cell lines U87, U251, and T98G were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Control

Journal: Pharmaceuticals

Article Title: N-Glycoside of Indolo[2,3- a ]pyrrolo[3,4- c ]carbazole LCS1269 Exerts Anti-Glioblastoma Effects by G2 Cell Cycle Arrest and CDK1 Activity Modulation: Molecular Docking Studies, Biological Investigations, and ADMET Prediction

doi: 10.3390/ph17121642

Figure Lengend Snippet: LCS1269 promotes G2 cell cycle block in glioblastoma cell lines. ( A ) Influence of LCS1269 different concentrations treatment (0.5, 1, and 2.5 µM) for 24 h on cell cycle progression using flow cytometry. ( B ) Quantification of cell cycle phase distribution after LCS1269 treatment for 24 h. ( C ) U87, U251, and T98G cells were treated with LCS1269 for 24 h, stained with Hoechst 33258 and visualized using fluorescence microscopy (scale bar = 10 µm). ( D ) Quantitative real-time PCR data of Aurora-B gene expression in glioblastoma cell lines treated with LCS1269 in the indicated concentration for 24 h. ( E ) Western blot analysis of p-Histone H3 (Ser10) protein level after LCS1269 treatment for 24 h. β-Actin served as a loading control. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 as compared with the untreated cells.

Article Snippet: The human glioblastoma multiforme (GBM) cell lines U87, U251, and T98G were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Blocking Assay, Flow Cytometry, Staining, Fluorescence, Microscopy, Real-time Polymerase Chain Reaction, Expressing, Concentration Assay, Western Blot, Control

Journal: Pharmaceuticals

Article Title: N-Glycoside of Indolo[2,3- a ]pyrrolo[3,4- c ]carbazole LCS1269 Exerts Anti-Glioblastoma Effects by G2 Cell Cycle Arrest and CDK1 Activity Modulation: Molecular Docking Studies, Biological Investigations, and ADMET Prediction

doi: 10.3390/ph17121642

Figure Lengend Snippet: LCS1269 partially affects CDK1 activity by regulation of Wee1/Myt1 and FOXM1/Plk1 signaling pathways, and p21 up-regulation. Western blot analysis of CDK1, p-CDK1, cyclin B1, Myt1, p-Wee1, Cdc25C, p-Cdc25C, p21, p27, FOXM1, Plk1, and p-Plk1 protein levels in U87, U251, and T98G cells treated with LCS1269 in indicated concentrations for 24 h. β-Actin served as a loading control.

Article Snippet: The human glioblastoma multiforme (GBM) cell lines U87, U251, and T98G were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activity Assay, Western Blot, Control