human glioblastoma multiforme gbm cell lines u87mg  (ATCC)

Journal: Pharmaceuticals

Article Title: N-Glycoside of Indolo[2,3- a ]pyrrolo[3,4- c ]carbazole LCS1269 Exerts Anti-Glioblastoma Effects by G2 Cell Cycle Arrest and CDK1 Activity Modulation: Molecular Docking Studies, Biological Investigations, and ADMET Prediction

doi: 10.3390/ph17121642

Figure Lengend Snippet: Inhibitory effect of LCS1269 on cell viability of human glioblastoma cell lines and patient-derived glioblastoma cell cultures. ( A – C ) The viability of three glioblastoma cell lines treated with the indicated concentrations of LCS1269 for 72 h. ( D ) IC 50 values of LCS1269 for U87, U251, and T98G cell lines. ( E – G ) LCS1269 treatment for 72 h reduces the viability of three patient-derived glioblastoma cell cultures in a dose-dependent manner. ( H ) IC 50 values of LCS1269 for Gbl1, Gbl2, and Gbl3 cell cultures. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 as compared with the untreated cells.

Article Snippet: The human glioblastoma multiforme (GBM) cell lines U87, U251, and T98G were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Derivative Assay

Journal: Pharmaceuticals

Article Title: N-Glycoside of Indolo[2,3- a ]pyrrolo[3,4- c ]carbazole LCS1269 Exerts Anti-Glioblastoma Effects by G2 Cell Cycle Arrest and CDK1 Activity Modulation: Molecular Docking Studies, Biological Investigations, and ADMET Prediction

doi: 10.3390/ph17121642

Figure Lengend Snippet: Inhibitory effect of conventional anti-glioblastoma drug temozolomide on cell viability of human glioblastoma cell lines and patient-derived glioblastoma cell cultures and therapeutic potency comparison of temozolomide and LCS1269. ( A – C ) The viability of three glioblastoma cell lines treated with the indicated concentrations of temozolomide for 72 h. ( D ) IC 50 values comparison of temozolomide and LCS1269 for U87, U251, and T98G cell lines. ( E – G ) temozolomide treatment for 72 h reduces the viability of three patient-derived glioblastoma cell cultures in a dose-dependent manner. ( H ) IC 50 values comparison of temozolomide and LCS1269 for Gbl1, Gbl2, and Gbl3 cell cultures. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 as compared with the untreated cells.

Article Snippet: The human glioblastoma multiforme (GBM) cell lines U87, U251, and T98G were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Derivative Assay, Comparison

Journal: Pharmaceuticals

Article Title: N-Glycoside of Indolo[2,3- a ]pyrrolo[3,4- c ]carbazole LCS1269 Exerts Anti-Glioblastoma Effects by G2 Cell Cycle Arrest and CDK1 Activity Modulation: Molecular Docking Studies, Biological Investigations, and ADMET Prediction

doi: 10.3390/ph17121642

Figure Lengend Snippet: Antitumor efficacy of LCS1269 against xenografts in U87-bearing nude mice ( n = 5). ( A ) Representative image of xenograft-bearing animals ( upper panel ) and extirpated tumors ( lower panel ) from control group. ( B ) Representative image of xenograft-bearing animals ( upper panel ) and extirpated tumors ( lower panel ) from LCS1269-treated group. ( C ) Body weight changes in nude mice from control and LCS1269-treated groups. ( D ) Changes in U87 xenograft volumes in control and LCS1269-treated groups of nude mice. Data are presented as mean ± SD.

Article Snippet: The human glioblastoma multiforme (GBM) cell lines U87, U251, and T98G were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Control

Journal: Pharmaceuticals

Article Title: N-Glycoside of Indolo[2,3- a ]pyrrolo[3,4- c ]carbazole LCS1269 Exerts Anti-Glioblastoma Effects by G2 Cell Cycle Arrest and CDK1 Activity Modulation: Molecular Docking Studies, Biological Investigations, and ADMET Prediction

doi: 10.3390/ph17121642

Figure Lengend Snippet: LCS1269 promotes G2 cell cycle block in glioblastoma cell lines. ( A ) Influence of LCS1269 different concentrations treatment (0.5, 1, and 2.5 µM) for 24 h on cell cycle progression using flow cytometry. ( B ) Quantification of cell cycle phase distribution after LCS1269 treatment for 24 h. ( C ) U87, U251, and T98G cells were treated with LCS1269 for 24 h, stained with Hoechst 33258 and visualized using fluorescence microscopy (scale bar = 10 µm). ( D ) Quantitative real-time PCR data of Aurora-B gene expression in glioblastoma cell lines treated with LCS1269 in the indicated concentration for 24 h. ( E ) Western blot analysis of p-Histone H3 (Ser10) protein level after LCS1269 treatment for 24 h. β-Actin served as a loading control. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 as compared with the untreated cells.

Article Snippet: The human glioblastoma multiforme (GBM) cell lines U87, U251, and T98G were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Blocking Assay, Flow Cytometry, Staining, Fluorescence, Microscopy, Real-time Polymerase Chain Reaction, Expressing, Concentration Assay, Western Blot, Control

Journal: Pharmaceuticals

Article Title: N-Glycoside of Indolo[2,3- a ]pyrrolo[3,4- c ]carbazole LCS1269 Exerts Anti-Glioblastoma Effects by G2 Cell Cycle Arrest and CDK1 Activity Modulation: Molecular Docking Studies, Biological Investigations, and ADMET Prediction

doi: 10.3390/ph17121642

Figure Lengend Snippet: LCS1269 partially affects CDK1 activity by regulation of Wee1/Myt1 and FOXM1/Plk1 signaling pathways, and p21 up-regulation. Western blot analysis of CDK1, p-CDK1, cyclin B1, Myt1, p-Wee1, Cdc25C, p-Cdc25C, p21, p27, FOXM1, Plk1, and p-Plk1 protein levels in U87, U251, and T98G cells treated with LCS1269 in indicated concentrations for 24 h. β-Actin served as a loading control.

Article Snippet: The human glioblastoma multiforme (GBM) cell lines U87, U251, and T98G were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activity Assay, Western Blot, Control

Journal: Frontiers in Oncology

Article Title: Combinatorial delivery of CPI444 and vatalanib loaded on PEGylated graphene oxide as an effective nanoformulation to target glioblastoma multiforme: In vitro evaluation

doi: 10.3389/fonc.2022.953098

Figure Lengend Snippet: Cell viability studies and cellular uptake of PEGylated graphene oxide. (A) CPI444 and vatalanib cell cytotoxicity were calculated in GBM U87 cells. Cells were treated with drugs individually along with different concentrations for 48 h. After 48 h, IC 50 values were calculated. (B) Cytotoxicity analyses of GO and GO-PEG were performed in GBM U87 cells. The PEGylated graphene oxide carrier did not show significant cytotoxicity in the GBM cell line up to 20 µg/ml. (C) In the GBM U87 cell line IC 50 value of CPI444 + vatalanib@GO-PEG is ~14 µM. (D) To confirm the cellular internalization of PEGylated GO nanoparticles in the GBM cells, we conducted a FACS study after 24 h of incubation with different concentrations of PEGylated graphene oxide nanoparticles; MFI (Mean Fluorescence Intensity) in arbitrary units (AU); **** p < 0.0001 and *** p < 0.001.

Article Snippet: Glioblastoma multiforme (Human) cancer cell line GBM U87 was cultured in Minimum Essential Media (MEM) containing 10% FBS and 1% antibiotic (penicillin/streptomycin) solution in a humidified cell culture incubator (Galaxy 170R, Eppendorf) containing 5% CO 2 at 37°C.

Techniques: Incubation, Fluorescence

Journal: Frontiers in Oncology

Article Title: Combinatorial delivery of CPI444 and vatalanib loaded on PEGylated graphene oxide as an effective nanoformulation to target glioblastoma multiforme: In vitro evaluation

doi: 10.3389/fonc.2022.953098

Figure Lengend Snippet: CPI444 and vatalanib-loaded GO-PEG affect the proliferation and stemness properties of GBM U87 cells. (A) Cellular calcium levels were estimated by FACS analysis using Fluo-4AM dye under different drug treatment conditions. (B) Colony forming assay of GBM U87 treated with untreated control, GO-PEG, CPI444 + vatalanib and CPI444 and vatalanib conjugated with GO-PEG. (C) Quantitation of colony forming assay, %RAU, relative absorbance units in percentage. (D) Heatmap depicting the expression levels of human pluripotent stem cell protein array for pluripotency and differentiation markers upon treatment with CPI444 and vatalanib loaded on GO-PEG with appropriate controls. MFI (Mean Fluorescence Intensity) in arbitrary units (AU); **** p < 0.0001 and * p < 0.05.

Article Snippet: Glioblastoma multiforme (Human) cancer cell line GBM U87 was cultured in Minimum Essential Media (MEM) containing 10% FBS and 1% antibiotic (penicillin/streptomycin) solution in a humidified cell culture incubator (Galaxy 170R, Eppendorf) containing 5% CO 2 at 37°C.

Techniques: Quantitation Assay, Expressing, Protein Array, Fluorescence

Journal: Frontiers in Oncology

Article Title: Combinatorial delivery of CPI444 and vatalanib loaded on PEGylated graphene oxide as an effective nanoformulation to target glioblastoma multiforme: In vitro evaluation

doi: 10.3389/fonc.2022.953098

Figure Lengend Snippet: CPI444 and vatalanib@GO-PEG hindered the cellular migration and invasive behavior of GBM U87 cells. (A) CD24 expression levels in GBM U87, quantitated by FACS analysis after treatment with CPI444 and vatalanib-loaded GO-PEG and other combinations. (B, C) Boyden chamber analysis (Transwell migration assay) was performed for cell migration properties. The combination of nanoconjugate CPI444 and vatalanib-treated cells shows smaller numbers of migrated cells compared to control. ( D) The adhesive property of GBM cells was significantly reduced after the treatment with the GO-PEG conjugated combination of CPI444 and vatalanib. RAU%, Relative absorbance units in percentage. ( E) Invadopodia causing dark gelatin degradation patches (indicated by white arrows) were detected by the gelatin degradation assay under different treatment conditions. **** p < 0.0001, *** p < 0.001, and ** p < 0.01.

Article Snippet: Glioblastoma multiforme (Human) cancer cell line GBM U87 was cultured in Minimum Essential Media (MEM) containing 10% FBS and 1% antibiotic (penicillin/streptomycin) solution in a humidified cell culture incubator (Galaxy 170R, Eppendorf) containing 5% CO 2 at 37°C.

Techniques: Migration, Expressing, Transwell Migration Assay, Degradation Assay

Journal: Frontiers in Oncology

Article Title: Combinatorial delivery of CPI444 and vatalanib loaded on PEGylated graphene oxide as an effective nanoformulation to target glioblastoma multiforme: In vitro evaluation

doi: 10.3389/fonc.2022.953098

Figure Lengend Snippet: Treatment of CPI444 and vatalanib-loaded GO-PEG inhibits the angiogenic potential and causes apoptosis of GBM U87. (A–C) A tube formation assay was performed for angiogenesis analysis in GBM cells. GBM U87 cells showed a smaller number of nodes and branches compared to control cells. (D–F) Western blot analysis of Bcl2, intact PARP, cleaved PARP. β-actin was used as an internal control. The Western blot shown is a representative of three independent experiments. **** p < 0.0001, *** p < 0.001, and ** p < 0.01.

Article Snippet: Glioblastoma multiforme (Human) cancer cell line GBM U87 was cultured in Minimum Essential Media (MEM) containing 10% FBS and 1% antibiotic (penicillin/streptomycin) solution in a humidified cell culture incubator (Galaxy 170R, Eppendorf) containing 5% CO 2 at 37°C.

Techniques: Tube Formation Assay, Western Blot