hffα human foreskin fibroblasts  (ATCC)


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    ATCC hffα human foreskin fibroblasts
    ( A, B ) MRC-5 (A) or HF99/7 (B) <t>fibroblasts</t> were nucleofected with US10-specific or non-targeting siRNA 24 hr prior to mock treatment or infection at an MOI of 5 with HCMV ΔUS2-6 mutant BAC2 or with AD169varL. At 48 h p.i., HLA-I surface expression was measured by flow cytometry using antibodies as indicated. Fold change by US10 was calculated as the ratio of the median fluorescence intensity (MFI) of cells treated with US10 siRNA compared to NT-treated cells. Dots represent individual values and bars mean values ± SEM from three independent experiments (biological replicates). Significance was calculated using one-way paired ANOVA followed by Dunnett’s multiple comparison test. ( C, D ) <t>HFFα</t> ( C ) or HFF99/7 ( D ) fibroblasts were treated and infected as in ( A, B ). At 24 h p.i., the fibroblasts were co-cultured for 5 hr with HLA-B*07:02pp65-specific polyclonal CD8+ T-cells gained from PBMCs (peripheral blood mononuclear cells) (C) or with an HLA-B*08IE1-specific CD8+ T-cell clone at an E/T ratio of 3:1 ( C ) and 5:1 ( D ), respectively. Activation of CD8+ T-cells was determined by intracellular IFNγ and TNFα stain. The percentage of IFNγ- or TNFα-expressing CD8+ T-cells was measured and the fold change by US10 siRNA was calculated. Dots represent individual values from two ( C ) and four ( D ) independent experiments (biological replicates). Co-culturing for ( D ) took place 24 (black dots) or 72 hr p.i. (gray dots). Representative dot plots are shown in .
    Hffα Human Foreskin Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Multimodal HLA-I genotype regulation by human cytomegalovirus US10 and resulting surface patterning"

    Article Title: Multimodal HLA-I genotype regulation by human cytomegalovirus US10 and resulting surface patterning

    Journal: eLife

    doi: 10.7554/eLife.85560

    ( A, B ) MRC-5 (A) or HF99/7 (B) fibroblasts were nucleofected with US10-specific or non-targeting siRNA 24 hr prior to mock treatment or infection at an MOI of 5 with HCMV ΔUS2-6 mutant BAC2 or with AD169varL. At 48 h p.i., HLA-I surface expression was measured by flow cytometry using antibodies as indicated. Fold change by US10 was calculated as the ratio of the median fluorescence intensity (MFI) of cells treated with US10 siRNA compared to NT-treated cells. Dots represent individual values and bars mean values ± SEM from three independent experiments (biological replicates). Significance was calculated using one-way paired ANOVA followed by Dunnett’s multiple comparison test. ( C, D ) HFFα ( C ) or HFF99/7 ( D ) fibroblasts were treated and infected as in ( A, B ). At 24 h p.i., the fibroblasts were co-cultured for 5 hr with HLA-B*07:02pp65-specific polyclonal CD8+ T-cells gained from PBMCs (peripheral blood mononuclear cells) (C) or with an HLA-B*08IE1-specific CD8+ T-cell clone at an E/T ratio of 3:1 ( C ) and 5:1 ( D ), respectively. Activation of CD8+ T-cells was determined by intracellular IFNγ and TNFα stain. The percentage of IFNγ- or TNFα-expressing CD8+ T-cells was measured and the fold change by US10 siRNA was calculated. Dots represent individual values from two ( C ) and four ( D ) independent experiments (biological replicates). Co-culturing for ( D ) took place 24 (black dots) or 72 hr p.i. (gray dots). Representative dot plots are shown in .
    Figure Legend Snippet: ( A, B ) MRC-5 (A) or HF99/7 (B) fibroblasts were nucleofected with US10-specific or non-targeting siRNA 24 hr prior to mock treatment or infection at an MOI of 5 with HCMV ΔUS2-6 mutant BAC2 or with AD169varL. At 48 h p.i., HLA-I surface expression was measured by flow cytometry using antibodies as indicated. Fold change by US10 was calculated as the ratio of the median fluorescence intensity (MFI) of cells treated with US10 siRNA compared to NT-treated cells. Dots represent individual values and bars mean values ± SEM from three independent experiments (biological replicates). Significance was calculated using one-way paired ANOVA followed by Dunnett’s multiple comparison test. ( C, D ) HFFα ( C ) or HFF99/7 ( D ) fibroblasts were treated and infected as in ( A, B ). At 24 h p.i., the fibroblasts were co-cultured for 5 hr with HLA-B*07:02pp65-specific polyclonal CD8+ T-cells gained from PBMCs (peripheral blood mononuclear cells) (C) or with an HLA-B*08IE1-specific CD8+ T-cell clone at an E/T ratio of 3:1 ( C ) and 5:1 ( D ), respectively. Activation of CD8+ T-cells was determined by intracellular IFNγ and TNFα stain. The percentage of IFNγ- or TNFα-expressing CD8+ T-cells was measured and the fold change by US10 siRNA was calculated. Dots represent individual values from two ( C ) and four ( D ) independent experiments (biological replicates). Co-culturing for ( D ) took place 24 (black dots) or 72 hr p.i. (gray dots). Representative dot plots are shown in .

    Techniques Used: Infection, Mutagenesis, Expressing, Flow Cytometry, Fluorescence, Comparison, Cell Culture, Activation Assay, Staining

    human foreskin fibroblast  (ATCC)


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    ATCC human foreskin fibroblast
    Human Foreskin Fibroblast, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human foreskin fibroblasts  (ATCC)


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    ATCC human foreskin fibroblasts
    Human Foreskin Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human foreskin fibroblasts  (ATCC)


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    ATCC human foreskin fibroblasts
    Human Foreskin Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human foreskin fibroblasts  (ATCC)


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    ATCC human foreskin fibroblasts
    Human Foreskin Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human foreskin fibroblasts  (ATCC)


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    ATCC human foreskin fibroblasts
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    human foreskin fibroblasts 1 hff1 cell line  (ATCC)


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    ATCC human foreskin fibroblasts 1 hff1 cell line
    Panel ( A ). Wound healing in <t>HFF1</t> cells treated for 24 h with 1% vol / vol S. telephium juices or bFGF (10 ng/mL). Representative images are reported. Images were acquired using an optical microscope equipped with a camera. Scale bar = 100 µm. Panel ( B ). The distance between cells at the edges of the scratch was measured using the software ImageJ (version 1.54h) and expressed as the percentage of closure of the area compared with cells incubated with the vehicle. Data are reported as mean ± SE of two independent experiments, each performed in triplicate. * denotes p < 0.05 vs. vehicle-treated cells; a denotes p < 0.05 vs. C1-J-treated cells, S1-J-treated cells, S2-J-treated cells, and bFGF-treated cells.
    Human Foreskin Fibroblasts 1 Hff1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "From Ethnobotany to Biotechnology: Wound Healing and Anti-Inflammatory Properties of Sedum telephium L. In Vitro Cultures"

    Article Title: From Ethnobotany to Biotechnology: Wound Healing and Anti-Inflammatory Properties of Sedum telephium L. In Vitro Cultures

    Journal: Molecules

    doi: 10.3390/molecules29112472

    Panel ( A ). Wound healing in HFF1 cells treated for 24 h with 1% vol / vol S. telephium juices or bFGF (10 ng/mL). Representative images are reported. Images were acquired using an optical microscope equipped with a camera. Scale bar = 100 µm. Panel ( B ). The distance between cells at the edges of the scratch was measured using the software ImageJ (version 1.54h) and expressed as the percentage of closure of the area compared with cells incubated with the vehicle. Data are reported as mean ± SE of two independent experiments, each performed in triplicate. * denotes p < 0.05 vs. vehicle-treated cells; a denotes p < 0.05 vs. C1-J-treated cells, S1-J-treated cells, S2-J-treated cells, and bFGF-treated cells.
    Figure Legend Snippet: Panel ( A ). Wound healing in HFF1 cells treated for 24 h with 1% vol / vol S. telephium juices or bFGF (10 ng/mL). Representative images are reported. Images were acquired using an optical microscope equipped with a camera. Scale bar = 100 µm. Panel ( B ). The distance between cells at the edges of the scratch was measured using the software ImageJ (version 1.54h) and expressed as the percentage of closure of the area compared with cells incubated with the vehicle. Data are reported as mean ± SE of two independent experiments, each performed in triplicate. * denotes p < 0.05 vs. vehicle-treated cells; a denotes p < 0.05 vs. C1-J-treated cells, S1-J-treated cells, S2-J-treated cells, and bFGF-treated cells.

    Techniques Used: Microscopy, Software, Incubation

    human foreskin fibroblasts  (ATCC)


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    ATCC human foreskin fibroblasts
    Ovarian cancer cell-conditioning medium (OCC CM) induces the phenoconversion of normal skin <t>fibroblasts</t> to CAFs. ( A ) Skin fibroblasts and HDFa and HFF-1 cell homogenates were analyzed by means of Western blotting for the expression of the CAF-markers FAP and α-SMA. The filter was probed with GAPDH as a loading control. ( B , C ) Skin fibroblasts were cultured with control medium (Co) or OCC CM collected from four different cancer cell lines (SKOV3, OVCAR3, OAW42, and Kuramochi) for 48 h. ( B ) Cells were fixed and stained for α-SMA (red) and FAP (green). The images were acquired with a fluorescence microscope and are representative of different fields per each condition. The average fluorescent integrated signal was determined using the ImageJ tool as described in the . Scale bar = 20 μm; magnification = 63×. ( C ) Cell homogenates were analyzed by means of Western blotting for the expression of FAP and α-SMA. The filter was probed with GAPDH as a loading control. The Western blotting is representative of three replicates, and densitometric data are reported in the graphs. Statistical analysis was performed using GraphPad Prism 5.0 software. Bonferroni’s multiple comparison test after one-way ANOVA analysis (unpaired, two-tailed) was employed. Significance was considered as follows: **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05.
    Human Foreskin Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Ovarian Cancer Cell-Conditioning Medium Induces Cancer-Associated Fibroblast Phenoconversion through Glucose-Dependent Inhibition of Autophagy"

    Article Title: Ovarian Cancer Cell-Conditioning Medium Induces Cancer-Associated Fibroblast Phenoconversion through Glucose-Dependent Inhibition of Autophagy

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25115691

    Ovarian cancer cell-conditioning medium (OCC CM) induces the phenoconversion of normal skin fibroblasts to CAFs. ( A ) Skin fibroblasts and HDFa and HFF-1 cell homogenates were analyzed by means of Western blotting for the expression of the CAF-markers FAP and α-SMA. The filter was probed with GAPDH as a loading control. ( B , C ) Skin fibroblasts were cultured with control medium (Co) or OCC CM collected from four different cancer cell lines (SKOV3, OVCAR3, OAW42, and Kuramochi) for 48 h. ( B ) Cells were fixed and stained for α-SMA (red) and FAP (green). The images were acquired with a fluorescence microscope and are representative of different fields per each condition. The average fluorescent integrated signal was determined using the ImageJ tool as described in the . Scale bar = 20 μm; magnification = 63×. ( C ) Cell homogenates were analyzed by means of Western blotting for the expression of FAP and α-SMA. The filter was probed with GAPDH as a loading control. The Western blotting is representative of three replicates, and densitometric data are reported in the graphs. Statistical analysis was performed using GraphPad Prism 5.0 software. Bonferroni’s multiple comparison test after one-way ANOVA analysis (unpaired, two-tailed) was employed. Significance was considered as follows: **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05.
    Figure Legend Snippet: Ovarian cancer cell-conditioning medium (OCC CM) induces the phenoconversion of normal skin fibroblasts to CAFs. ( A ) Skin fibroblasts and HDFa and HFF-1 cell homogenates were analyzed by means of Western blotting for the expression of the CAF-markers FAP and α-SMA. The filter was probed with GAPDH as a loading control. ( B , C ) Skin fibroblasts were cultured with control medium (Co) or OCC CM collected from four different cancer cell lines (SKOV3, OVCAR3, OAW42, and Kuramochi) for 48 h. ( B ) Cells were fixed and stained for α-SMA (red) and FAP (green). The images were acquired with a fluorescence microscope and are representative of different fields per each condition. The average fluorescent integrated signal was determined using the ImageJ tool as described in the . Scale bar = 20 μm; magnification = 63×. ( C ) Cell homogenates were analyzed by means of Western blotting for the expression of FAP and α-SMA. The filter was probed with GAPDH as a loading control. The Western blotting is representative of three replicates, and densitometric data are reported in the graphs. Statistical analysis was performed using GraphPad Prism 5.0 software. Bonferroni’s multiple comparison test after one-way ANOVA analysis (unpaired, two-tailed) was employed. Significance was considered as follows: **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05.

    Techniques Used: Western Blot, Expressing, Cell Culture, Staining, Fluorescence, Microscopy, Software, Comparison, Two Tailed Test

    Skin fibroblasts, HDFa, and HFF-1 differ in their basal autophagy. ( A ) Skin fibroblasts, HDFa, and HFF-1 were cultured in the absence/presence of 30 µM chloroquine (ClQ) for 8 h. Cell homogenates were analyzed by means of Western blotting for the expression of the autophagic markers p62 and LC3. The filter was probed with GAPDH as a loading control. ( B – D ) The three fibroblast cell models were cultured with control medium (Co) or OCC CM collected from four different cancer cell lines (SKOV3, OVCAR3, OAW42, and Kuramochi) for 48 h, as previously mentioned. Cell homogenates from skin fibroblasts ( B ), HDFa ( C ), and HFF-1 ( D ) were analyzed by means of Western blotting for the expression of p62 and LC3. The filters were probed with β-actin as a loading control. Autophagy flux was monitored by the conversion rate of the cytosolic immature form LC3-I to the lipidated mature form LC3-II and by p62 levels. The densitometric data of the triplicates are reported in the graphs. Statistical analysis was performed using GraphPad Prism 5.0 software. Bonferroni’s multiple comparison test after one-way ANOVA analysis (unpaired, two-tailed) was employed. Significance was considered as follows: *** p < 0.001; ** p < 0.01; * p < 0.05.
    Figure Legend Snippet: Skin fibroblasts, HDFa, and HFF-1 differ in their basal autophagy. ( A ) Skin fibroblasts, HDFa, and HFF-1 were cultured in the absence/presence of 30 µM chloroquine (ClQ) for 8 h. Cell homogenates were analyzed by means of Western blotting for the expression of the autophagic markers p62 and LC3. The filter was probed with GAPDH as a loading control. ( B – D ) The three fibroblast cell models were cultured with control medium (Co) or OCC CM collected from four different cancer cell lines (SKOV3, OVCAR3, OAW42, and Kuramochi) for 48 h, as previously mentioned. Cell homogenates from skin fibroblasts ( B ), HDFa ( C ), and HFF-1 ( D ) were analyzed by means of Western blotting for the expression of p62 and LC3. The filters were probed with β-actin as a loading control. Autophagy flux was monitored by the conversion rate of the cytosolic immature form LC3-I to the lipidated mature form LC3-II and by p62 levels. The densitometric data of the triplicates are reported in the graphs. Statistical analysis was performed using GraphPad Prism 5.0 software. Bonferroni’s multiple comparison test after one-way ANOVA analysis (unpaired, two-tailed) was employed. Significance was considered as follows: *** p < 0.001; ** p < 0.01; * p < 0.05.

    Techniques Used: Cell Culture, Western Blot, Expressing, Software, Comparison, Two Tailed Test

    human foreskin fibroblasts  (ATCC)


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    ATCC human foreskin fibroblasts
    Human Foreskin Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human foreskin fibroblast cell hff  (ATCC)


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    ATCC human foreskin fibroblast cell hff
    Human Foreskin Fibroblast Cell Hff, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC hffα human foreskin fibroblasts
    ( A, B ) MRC-5 (A) or HF99/7 (B) <t>fibroblasts</t> were nucleofected with US10-specific or non-targeting siRNA 24 hr prior to mock treatment or infection at an MOI of 5 with HCMV ΔUS2-6 mutant BAC2 or with AD169varL. At 48 h p.i., HLA-I surface expression was measured by flow cytometry using antibodies as indicated. Fold change by US10 was calculated as the ratio of the median fluorescence intensity (MFI) of cells treated with US10 siRNA compared to NT-treated cells. Dots represent individual values and bars mean values ± SEM from three independent experiments (biological replicates). Significance was calculated using one-way paired ANOVA followed by Dunnett’s multiple comparison test. ( C, D ) <t>HFFα</t> ( C ) or HFF99/7 ( D ) fibroblasts were treated and infected as in ( A, B ). At 24 h p.i., the fibroblasts were co-cultured for 5 hr with HLA-B*07:02pp65-specific polyclonal CD8+ T-cells gained from PBMCs (peripheral blood mononuclear cells) (C) or with an HLA-B*08IE1-specific CD8+ T-cell clone at an E/T ratio of 3:1 ( C ) and 5:1 ( D ), respectively. Activation of CD8+ T-cells was determined by intracellular IFNγ and TNFα stain. The percentage of IFNγ- or TNFα-expressing CD8+ T-cells was measured and the fold change by US10 siRNA was calculated. Dots represent individual values from two ( C ) and four ( D ) independent experiments (biological replicates). Co-culturing for ( D ) took place 24 (black dots) or 72 hr p.i. (gray dots). Representative dot plots are shown in .
    Hffα Human Foreskin Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hffα human foreskin fibroblasts/product/ATCC
    Average 86 stars, based on 1 article reviews
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    ATCC human foreskin fibroblast
    ( A, B ) MRC-5 (A) or HF99/7 (B) <t>fibroblasts</t> were nucleofected with US10-specific or non-targeting siRNA 24 hr prior to mock treatment or infection at an MOI of 5 with HCMV ΔUS2-6 mutant BAC2 or with AD169varL. At 48 h p.i., HLA-I surface expression was measured by flow cytometry using antibodies as indicated. Fold change by US10 was calculated as the ratio of the median fluorescence intensity (MFI) of cells treated with US10 siRNA compared to NT-treated cells. Dots represent individual values and bars mean values ± SEM from three independent experiments (biological replicates). Significance was calculated using one-way paired ANOVA followed by Dunnett’s multiple comparison test. ( C, D ) <t>HFFα</t> ( C ) or HFF99/7 ( D ) fibroblasts were treated and infected as in ( A, B ). At 24 h p.i., the fibroblasts were co-cultured for 5 hr with HLA-B*07:02pp65-specific polyclonal CD8+ T-cells gained from PBMCs (peripheral blood mononuclear cells) (C) or with an HLA-B*08IE1-specific CD8+ T-cell clone at an E/T ratio of 3:1 ( C ) and 5:1 ( D ), respectively. Activation of CD8+ T-cells was determined by intracellular IFNγ and TNFα stain. The percentage of IFNγ- or TNFα-expressing CD8+ T-cells was measured and the fold change by US10 siRNA was calculated. Dots represent individual values from two ( C ) and four ( D ) independent experiments (biological replicates). Co-culturing for ( D ) took place 24 (black dots) or 72 hr p.i. (gray dots). Representative dot plots are shown in .
    Human Foreskin Fibroblast, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human foreskin fibroblast/product/ATCC
    Average 86 stars, based on 1 article reviews
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    ATCC human foreskin fibroblasts
    ( A, B ) MRC-5 (A) or HF99/7 (B) <t>fibroblasts</t> were nucleofected with US10-specific or non-targeting siRNA 24 hr prior to mock treatment or infection at an MOI of 5 with HCMV ΔUS2-6 mutant BAC2 or with AD169varL. At 48 h p.i., HLA-I surface expression was measured by flow cytometry using antibodies as indicated. Fold change by US10 was calculated as the ratio of the median fluorescence intensity (MFI) of cells treated with US10 siRNA compared to NT-treated cells. Dots represent individual values and bars mean values ± SEM from three independent experiments (biological replicates). Significance was calculated using one-way paired ANOVA followed by Dunnett’s multiple comparison test. ( C, D ) <t>HFFα</t> ( C ) or HFF99/7 ( D ) fibroblasts were treated and infected as in ( A, B ). At 24 h p.i., the fibroblasts were co-cultured for 5 hr with HLA-B*07:02pp65-specific polyclonal CD8+ T-cells gained from PBMCs (peripheral blood mononuclear cells) (C) or with an HLA-B*08IE1-specific CD8+ T-cell clone at an E/T ratio of 3:1 ( C ) and 5:1 ( D ), respectively. Activation of CD8+ T-cells was determined by intracellular IFNγ and TNFα stain. The percentage of IFNγ- or TNFα-expressing CD8+ T-cells was measured and the fold change by US10 siRNA was calculated. Dots represent individual values from two ( C ) and four ( D ) independent experiments (biological replicates). Co-culturing for ( D ) took place 24 (black dots) or 72 hr p.i. (gray dots). Representative dot plots are shown in .
    Human Foreskin Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human foreskin fibroblasts/product/ATCC
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    ATCC human foreskin fibroblasts 1 hff1 cell line
    Panel ( A ). Wound healing in <t>HFF1</t> cells treated for 24 h with 1% vol / vol S. telephium juices or bFGF (10 ng/mL). Representative images are reported. Images were acquired using an optical microscope equipped with a camera. Scale bar = 100 µm. Panel ( B ). The distance between cells at the edges of the scratch was measured using the software ImageJ (version 1.54h) and expressed as the percentage of closure of the area compared with cells incubated with the vehicle. Data are reported as mean ± SE of two independent experiments, each performed in triplicate. * denotes p < 0.05 vs. vehicle-treated cells; a denotes p < 0.05 vs. C1-J-treated cells, S1-J-treated cells, S2-J-treated cells, and bFGF-treated cells.
    Human Foreskin Fibroblasts 1 Hff1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human foreskin fibroblasts 1 hff1 cell line/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human foreskin fibroblasts 1 hff1 cell line - by Bioz Stars, 2024-06
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    ATCC human foreskin fibroblast cell hff
    Panel ( A ). Wound healing in <t>HFF1</t> cells treated for 24 h with 1% vol / vol S. telephium juices or bFGF (10 ng/mL). Representative images are reported. Images were acquired using an optical microscope equipped with a camera. Scale bar = 100 µm. Panel ( B ). The distance between cells at the edges of the scratch was measured using the software ImageJ (version 1.54h) and expressed as the percentage of closure of the area compared with cells incubated with the vehicle. Data are reported as mean ± SE of two independent experiments, each performed in triplicate. * denotes p < 0.05 vs. vehicle-treated cells; a denotes p < 0.05 vs. C1-J-treated cells, S1-J-treated cells, S2-J-treated cells, and bFGF-treated cells.
    Human Foreskin Fibroblast Cell Hff, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human foreskin fibroblast cell hff/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human foreskin fibroblast cell hff - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    Image Search Results


    ( A, B ) MRC-5 (A) or HF99/7 (B) fibroblasts were nucleofected with US10-specific or non-targeting siRNA 24 hr prior to mock treatment or infection at an MOI of 5 with HCMV ΔUS2-6 mutant BAC2 or with AD169varL. At 48 h p.i., HLA-I surface expression was measured by flow cytometry using antibodies as indicated. Fold change by US10 was calculated as the ratio of the median fluorescence intensity (MFI) of cells treated with US10 siRNA compared to NT-treated cells. Dots represent individual values and bars mean values ± SEM from three independent experiments (biological replicates). Significance was calculated using one-way paired ANOVA followed by Dunnett’s multiple comparison test. ( C, D ) HFFα ( C ) or HFF99/7 ( D ) fibroblasts were treated and infected as in ( A, B ). At 24 h p.i., the fibroblasts were co-cultured for 5 hr with HLA-B*07:02pp65-specific polyclonal CD8+ T-cells gained from PBMCs (peripheral blood mononuclear cells) (C) or with an HLA-B*08IE1-specific CD8+ T-cell clone at an E/T ratio of 3:1 ( C ) and 5:1 ( D ), respectively. Activation of CD8+ T-cells was determined by intracellular IFNγ and TNFα stain. The percentage of IFNγ- or TNFα-expressing CD8+ T-cells was measured and the fold change by US10 siRNA was calculated. Dots represent individual values from two ( C ) and four ( D ) independent experiments (biological replicates). Co-culturing for ( D ) took place 24 (black dots) or 72 hr p.i. (gray dots). Representative dot plots are shown in .

    Journal: eLife

    Article Title: Multimodal HLA-I genotype regulation by human cytomegalovirus US10 and resulting surface patterning

    doi: 10.7554/eLife.85560

    Figure Lengend Snippet: ( A, B ) MRC-5 (A) or HF99/7 (B) fibroblasts were nucleofected with US10-specific or non-targeting siRNA 24 hr prior to mock treatment or infection at an MOI of 5 with HCMV ΔUS2-6 mutant BAC2 or with AD169varL. At 48 h p.i., HLA-I surface expression was measured by flow cytometry using antibodies as indicated. Fold change by US10 was calculated as the ratio of the median fluorescence intensity (MFI) of cells treated with US10 siRNA compared to NT-treated cells. Dots represent individual values and bars mean values ± SEM from three independent experiments (biological replicates). Significance was calculated using one-way paired ANOVA followed by Dunnett’s multiple comparison test. ( C, D ) HFFα ( C ) or HFF99/7 ( D ) fibroblasts were treated and infected as in ( A, B ). At 24 h p.i., the fibroblasts were co-cultured for 5 hr with HLA-B*07:02pp65-specific polyclonal CD8+ T-cells gained from PBMCs (peripheral blood mononuclear cells) (C) or with an HLA-B*08IE1-specific CD8+ T-cell clone at an E/T ratio of 3:1 ( C ) and 5:1 ( D ), respectively. Activation of CD8+ T-cells was determined by intracellular IFNγ and TNFα stain. The percentage of IFNγ- or TNFα-expressing CD8+ T-cells was measured and the fold change by US10 siRNA was calculated. Dots represent individual values from two ( C ) and four ( D ) independent experiments (biological replicates). Co-culturing for ( D ) took place 24 (black dots) or 72 hr p.i. (gray dots). Representative dot plots are shown in .

    Article Snippet: HeLa cells (human, ATCC CCL-2), MRC-5 fibroblasts (human, ATCC CCL-171), BJ-5ta (hTERT; human, ATCC-CRL-4001), and HFFα human foreskin fibroblasts (a kind gift from Dieter Neumann-Haefelin and Valeria Kapper-Falcone, Institute of Virology, Medical Center University of Freiburg, Freiburg, Germany) were grown in DMEM (Life Technologies) supplemented with 10% (v/v) FCS (Biochrom, Sigma-Aldrich or PAN-Biotech) and 1% (v/v) penicillin/streptomycin (Life Technologies, stock: 5000 U/mL) at 37°C and 5% CO 2 .

    Techniques: Infection, Mutagenesis, Expressing, Flow Cytometry, Fluorescence, Comparison, Cell Culture, Activation Assay, Staining

    Panel ( A ). Wound healing in HFF1 cells treated for 24 h with 1% vol / vol S. telephium juices or bFGF (10 ng/mL). Representative images are reported. Images were acquired using an optical microscope equipped with a camera. Scale bar = 100 µm. Panel ( B ). The distance between cells at the edges of the scratch was measured using the software ImageJ (version 1.54h) and expressed as the percentage of closure of the area compared with cells incubated with the vehicle. Data are reported as mean ± SE of two independent experiments, each performed in triplicate. * denotes p < 0.05 vs. vehicle-treated cells; a denotes p < 0.05 vs. C1-J-treated cells, S1-J-treated cells, S2-J-treated cells, and bFGF-treated cells.

    Journal: Molecules

    Article Title: From Ethnobotany to Biotechnology: Wound Healing and Anti-Inflammatory Properties of Sedum telephium L. In Vitro Cultures

    doi: 10.3390/molecules29112472

    Figure Lengend Snippet: Panel ( A ). Wound healing in HFF1 cells treated for 24 h with 1% vol / vol S. telephium juices or bFGF (10 ng/mL). Representative images are reported. Images were acquired using an optical microscope equipped with a camera. Scale bar = 100 µm. Panel ( B ). The distance between cells at the edges of the scratch was measured using the software ImageJ (version 1.54h) and expressed as the percentage of closure of the area compared with cells incubated with the vehicle. Data are reported as mean ± SE of two independent experiments, each performed in triplicate. * denotes p < 0.05 vs. vehicle-treated cells; a denotes p < 0.05 vs. C1-J-treated cells, S1-J-treated cells, S2-J-treated cells, and bFGF-treated cells.

    Article Snippet: The human foreskin fibroblasts 1 (HFF1) cell line was purchased from the American Type Culture Collection (ATCC, LGC Standards S.r.l., Sesto San Giovanni, Italy) and routinely cultured in DMEM supplemented with 1% ( vol / vol ) penicillin and streptomycin, 2 mM L-glutamine, and 10% ( vol / vol ) heat-inactivated FBS (all purchased from Life Technologies).

    Techniques: Microscopy, Software, Incubation