human foreskin fibroblasts hff cell line  (Pasteur Institute)

Journal: Molecules

Article Title: From Ethnobotany to Biotechnology: Wound Healing and Anti-Inflammatory Properties of Sedum telephium L. In Vitro Cultures

doi: 10.3390/molecules29112472

Figure Lengend Snippet: Panel ( A ). Wound healing in HFF1 cells treated for 24 h with 1% vol / vol S. telephium juices or bFGF (10 ng/mL). Representative images are reported. Images were acquired using an optical microscope equipped with a camera. Scale bar = 100 µm. Panel ( B ). The distance between cells at the edges of the scratch was measured using the software ImageJ (version 1.54h) and expressed as the percentage of closure of the area compared with cells incubated with the vehicle. Data are reported as mean ± SE of two independent experiments, each performed in triplicate. * denotes p < 0.05 vs. vehicle-treated cells; a denotes p < 0.05 vs. C1-J-treated cells, S1-J-treated cells, S2-J-treated cells, and bFGF-treated cells.

Article Snippet: The human foreskin fibroblasts 1 (HFF1) cell line was purchased from the American Type Culture Collection (ATCC, LGC Standards S.r.l., Sesto San Giovanni, Italy) and routinely cultured in DMEM supplemented with 1% ( vol / vol ) penicillin and streptomycin, 2 mM L-glutamine, and 10% ( vol / vol ) heat-inactivated FBS (all purchased from Life Technologies).

Techniques: Microscopy, Software, Incubation